Effectiveness of mechanical brushing with different denture cleansing agents in reducing in vitro Candida albicans biofilm viability
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Journal |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402012000500013 |
Resumo: | The adhesion of Candida albicans to surfaces is the prerequisite for occurrence of denture stomatitis, a common disease diagnosed among denture wearers. A routine of denture cleansing is essential to prevent biofilm formation and the onset of this infection. The aim of this study was to investigate the effectiveness of combining brushing and cleansing agents in killing C. albicans biofilm. Disks of acrylic resin were made, sterilized, and inoculated with C. albicans (10(7) cfu/mL). After incubation (37°C/48 h), specimens were randomly assigned to 10 experimental groups (n=9): 5 subjected to brushing with distilled water or cleansing agents - dentifrice slurry, 2% chlorhexidine gluconate (CHX), 1% sodium hypochlorite (NaOCl), and Polident fresh cleanse® (combined method) - and 4 exposed to the cleansing agents without brushing (immersion). Non-cleansed specimens were used as positive controls. The viability of cells was evaluated by XTT reduction method. Results were analyzed by Mann-Whitney and Kruskal-Wallis tests (α=0.05). The combined method was significantly more effective (p<0.0001) in reducing biofilm viability than the immersion. Brushing with CHX and NaOCl resulted in 100% removal of the biofilm. Immersion in the agents reduced significantly (p<0.0001) the biofilm viability, with CHX being the most effective (p<0.0001). The use of the combined method of brushing with cleansing agents is an effective method to reduce C. albicans biofilm, being CHX and NaOCl the most effective solutions. |
id |
FUNORP-1_bd666b23dd64ddaa508aadced2463a11 |
---|---|
oai_identifier_str |
oai:scielo:S0103-64402012000500013 |
network_acronym_str |
FUNORP-1 |
network_name_str |
Brazilian Dental Journal |
repository_id_str |
|
spelling |
Effectiveness of mechanical brushing with different denture cleansing agents in reducing in vitro Candida albicans biofilm viabilityBiofilmsCandida albicansbrushingdisinfectantsacrylic resinsdenture cleansersoral hygieneThe adhesion of Candida albicans to surfaces is the prerequisite for occurrence of denture stomatitis, a common disease diagnosed among denture wearers. A routine of denture cleansing is essential to prevent biofilm formation and the onset of this infection. The aim of this study was to investigate the effectiveness of combining brushing and cleansing agents in killing C. albicans biofilm. Disks of acrylic resin were made, sterilized, and inoculated with C. albicans (10(7) cfu/mL). After incubation (37°C/48 h), specimens were randomly assigned to 10 experimental groups (n=9): 5 subjected to brushing with distilled water or cleansing agents - dentifrice slurry, 2% chlorhexidine gluconate (CHX), 1% sodium hypochlorite (NaOCl), and Polident fresh cleanse® (combined method) - and 4 exposed to the cleansing agents without brushing (immersion). Non-cleansed specimens were used as positive controls. The viability of cells was evaluated by XTT reduction method. Results were analyzed by Mann-Whitney and Kruskal-Wallis tests (α=0.05). The combined method was significantly more effective (p<0.0001) in reducing biofilm viability than the immersion. Brushing with CHX and NaOCl resulted in 100% removal of the biofilm. Immersion in the agents reduced significantly (p<0.0001) the biofilm viability, with CHX being the most effective (p<0.0001). The use of the combined method of brushing with cleansing agents is an effective method to reduce C. albicans biofilm, being CHX and NaOCl the most effective solutions.Fundação Odontológica de Ribeirão Preto2012-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402012000500013Brazilian Dental Journal v.23 n.5 2012reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/S0103-64402012000500013info:eu-repo/semantics/openAccessPellizzaro,DelisePolyzois,GregoryMachado,Ana LuciaGiampaolo,Eunice TeresinhaSanitá,Paula VolpatoVergani,Carlos Eduardoeng2013-01-08T00:00:00Zoai:scielo:S0103-64402012000500013Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2013-01-08T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false |
dc.title.none.fl_str_mv |
Effectiveness of mechanical brushing with different denture cleansing agents in reducing in vitro Candida albicans biofilm viability |
title |
Effectiveness of mechanical brushing with different denture cleansing agents in reducing in vitro Candida albicans biofilm viability |
spellingShingle |
Effectiveness of mechanical brushing with different denture cleansing agents in reducing in vitro Candida albicans biofilm viability Pellizzaro,Delise Biofilms Candida albicans brushing disinfectants acrylic resins denture cleansers oral hygiene |
title_short |
Effectiveness of mechanical brushing with different denture cleansing agents in reducing in vitro Candida albicans biofilm viability |
title_full |
Effectiveness of mechanical brushing with different denture cleansing agents in reducing in vitro Candida albicans biofilm viability |
title_fullStr |
Effectiveness of mechanical brushing with different denture cleansing agents in reducing in vitro Candida albicans biofilm viability |
title_full_unstemmed |
Effectiveness of mechanical brushing with different denture cleansing agents in reducing in vitro Candida albicans biofilm viability |
title_sort |
Effectiveness of mechanical brushing with different denture cleansing agents in reducing in vitro Candida albicans biofilm viability |
author |
Pellizzaro,Delise |
author_facet |
Pellizzaro,Delise Polyzois,Gregory Machado,Ana Lucia Giampaolo,Eunice Teresinha Sanitá,Paula Volpato Vergani,Carlos Eduardo |
author_role |
author |
author2 |
Polyzois,Gregory Machado,Ana Lucia Giampaolo,Eunice Teresinha Sanitá,Paula Volpato Vergani,Carlos Eduardo |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Pellizzaro,Delise Polyzois,Gregory Machado,Ana Lucia Giampaolo,Eunice Teresinha Sanitá,Paula Volpato Vergani,Carlos Eduardo |
dc.subject.por.fl_str_mv |
Biofilms Candida albicans brushing disinfectants acrylic resins denture cleansers oral hygiene |
topic |
Biofilms Candida albicans brushing disinfectants acrylic resins denture cleansers oral hygiene |
description |
The adhesion of Candida albicans to surfaces is the prerequisite for occurrence of denture stomatitis, a common disease diagnosed among denture wearers. A routine of denture cleansing is essential to prevent biofilm formation and the onset of this infection. The aim of this study was to investigate the effectiveness of combining brushing and cleansing agents in killing C. albicans biofilm. Disks of acrylic resin were made, sterilized, and inoculated with C. albicans (10(7) cfu/mL). After incubation (37°C/48 h), specimens were randomly assigned to 10 experimental groups (n=9): 5 subjected to brushing with distilled water or cleansing agents - dentifrice slurry, 2% chlorhexidine gluconate (CHX), 1% sodium hypochlorite (NaOCl), and Polident fresh cleanse® (combined method) - and 4 exposed to the cleansing agents without brushing (immersion). Non-cleansed specimens were used as positive controls. The viability of cells was evaluated by XTT reduction method. Results were analyzed by Mann-Whitney and Kruskal-Wallis tests (α=0.05). The combined method was significantly more effective (p<0.0001) in reducing biofilm viability than the immersion. Brushing with CHX and NaOCl resulted in 100% removal of the biofilm. Immersion in the agents reduced significantly (p<0.0001) the biofilm viability, with CHX being the most effective (p<0.0001). The use of the combined method of brushing with cleansing agents is an effective method to reduce C. albicans biofilm, being CHX and NaOCl the most effective solutions. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402012000500013 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402012000500013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-64402012000500013 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
dc.source.none.fl_str_mv |
Brazilian Dental Journal v.23 n.5 2012 reponame:Brazilian Dental Journal instname:Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP |
instname_str |
Fundação Odontológica de Ribeirão Preto (FUNORP) |
instacron_str |
FUNORP |
institution |
FUNORP |
reponame_str |
Brazilian Dental Journal |
collection |
Brazilian Dental Journal |
repository.name.fl_str_mv |
Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP) |
repository.mail.fl_str_mv |
bdj@forp.usp.br||sergio@fosjc.unesp.br |
_version_ |
1754204092258844672 |