PROTEIN ISOLATE OF RICE FLOUR: CHARACTERIZATION E FUNCTIONAL PROPERTIES

Detalhes bibliográficos
Autor(a) principal: Latorres, Juliana Machado
Data de Publicação: 2016
Outros Autores: Paiva, Ana Raisa, Gonzales, Bruna Araújo, Mellado, Myriam Salas
Tipo de documento: Artigo
Idioma: por
Título da fonte: Vetor (Online)
Texto Completo: https://periodicos.furg.br/vetor/article/view/2240
Resumo: The aim of this study was to isolate proteins from rice flour using an enzymatic method with the enzyme Termamyl®. The functional properties of solubility, water and oil holding capacity, emulsifying capacity, foam property and "in vitro" digestibility in flour and isolate were determined. The protein isolate was obtained after the reaction with the enzyme Termamyl® during 90 minutes at 90 ºC. The slurry was then filtered and dried at 35 ºC during 24 hours, obtaining 91.2 % of protein (dry basis) in the isolate. The values of the functional properties presented by the rice flour were: 17.8 % solubility at pH 10 and 6.2 % at pH 5; 1.13 mL oil/g of protein of emulsifying capacity; 2.46 g/g of sample of water holding capacity; oil holding capacity of 1.61 g/g of sample, and "in vitro" digestibility of 57.6 %. The protein isolate presented the following values: solubility of 7.4 % at pH 11 and 1.1 % at pH of 4.5; emulsifying capacity of 3 mL oil/g of protein, water holding capacity of 5.4 g/g of sample and oil holding capacity of 6.3 g/g of sample and "in vitro" digestibility of 83.6 %. All the isolate functional properties values, except the solubility, were better than the raw material values.
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spelling PROTEIN ISOLATE OF RICE FLOUR: CHARACTERIZATION E FUNCTIONAL PROPERTIESIsolado protéico de farinha de arroz: caracterização e propriedades funcionaisEngenhariasCiências AgráriasAlimentosThe aim of this study was to isolate proteins from rice flour using an enzymatic method with the enzyme Termamyl®. The functional properties of solubility, water and oil holding capacity, emulsifying capacity, foam property and "in vitro" digestibility in flour and isolate were determined. The protein isolate was obtained after the reaction with the enzyme Termamyl® during 90 minutes at 90 ºC. The slurry was then filtered and dried at 35 ºC during 24 hours, obtaining 91.2 % of protein (dry basis) in the isolate. The values of the functional properties presented by the rice flour were: 17.8 % solubility at pH 10 and 6.2 % at pH 5; 1.13 mL oil/g of protein of emulsifying capacity; 2.46 g/g of sample of water holding capacity; oil holding capacity of 1.61 g/g of sample, and "in vitro" digestibility of 57.6 %. The protein isolate presented the following values: solubility of 7.4 % at pH 11 and 1.1 % at pH of 4.5; emulsifying capacity of 3 mL oil/g of protein, water holding capacity of 5.4 g/g of sample and oil holding capacity of 6.3 g/g of sample and "in vitro" digestibility of 83.6 %. All the isolate functional properties values, except the solubility, were better than the raw material values.O presente trabalho teve como objetivo isolar as proteínas da farinha de arroz pelo método enzimático, utilizando a enzima Termamyl®. Foram determinadas as propriedades funcionais de solubilidade, capacidade de retenção de água e óleo, capacidade emulsificante, propriedades espumantes e digestibilidade “in vitro” na farinha e no isolado protéico de arroz. O isolado protéico foi obtido após a reação com a enzima Termamyl® durante 90 minutos a 97°C e posterior separação das proteínas por filtração e secagem a 35 °C por 24 horas obtendo um teor de proteína de 91,2 % em base seca, no isolado. Os valores das propriedades funcionais da farinha de arroz foram: solubilidade de 17,8 % em pH 10 e 6,2 % em pH 5; capacidade emulsificante de 1,13 mL de óleo/g de proteína, capacidade de absorção de água de 2,46 g/g de amostra, capacidade de retenção de óleo de 1,61 g/g amostra e digestibilidade “in vitro” da proteína de 57,6 %. O isolado protéico apresentou solubilidade de 7,4 % em pH 11 e 1,1% em pH 4,5; capacidade emulsificante de 3mL de óleo/g de proteína, capacidade de absorção de água de 5,4 g/g de amostra, capacidade de retenção de óleo de 6,3 g/g amostra e digestibilidade “in vitro” da proteína de 83, 6 %. Todas as propriedades funcionais, exceto a solubilidade, foram melhoradas no isolado, quando comparado com a matéria-prima.Universidade Federal do Rio Grande2016-09-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.furg.br/vetor/article/view/2240VETOR - Journal of Exact Sciences and Engineering; Vol. 24 No. 1 (2014); 53-65VETOR - Revista de Ciências Exatas e Engenharias; v. 24 n. 1 (2014); 53-652358-34520102-7352reponame:Vetor (Online)instname:Universidade Federal do Rio Grande (FURG)instacron:FURGporhttps://periodicos.furg.br/vetor/article/view/2240/3803Copyright (c) 2016 VETOR - Revista de Ciências Exatas e Engenhariasinfo:eu-repo/semantics/openAccessLatorres, Juliana MachadoPaiva, Ana RaisaGonzales, Bruna AraújoMellado, Myriam Salas2016-09-23T00:56:36Zoai:periodicos.furg.br:article/2240Revistahttps://periodicos.furg.br/vetorPUBhttps://periodicos.furg.br/vetor/oaigmplatt@furg.br2358-34520102-7352opendoar:2016-09-23T00:56:36Vetor (Online) - Universidade Federal do Rio Grande (FURG)false
dc.title.none.fl_str_mv PROTEIN ISOLATE OF RICE FLOUR: CHARACTERIZATION E FUNCTIONAL PROPERTIES
Isolado protéico de farinha de arroz: caracterização e propriedades funcionais
title PROTEIN ISOLATE OF RICE FLOUR: CHARACTERIZATION E FUNCTIONAL PROPERTIES
spellingShingle PROTEIN ISOLATE OF RICE FLOUR: CHARACTERIZATION E FUNCTIONAL PROPERTIES
Latorres, Juliana Machado
Engenharias
Ciências Agrárias
Alimentos
title_short PROTEIN ISOLATE OF RICE FLOUR: CHARACTERIZATION E FUNCTIONAL PROPERTIES
title_full PROTEIN ISOLATE OF RICE FLOUR: CHARACTERIZATION E FUNCTIONAL PROPERTIES
title_fullStr PROTEIN ISOLATE OF RICE FLOUR: CHARACTERIZATION E FUNCTIONAL PROPERTIES
title_full_unstemmed PROTEIN ISOLATE OF RICE FLOUR: CHARACTERIZATION E FUNCTIONAL PROPERTIES
title_sort PROTEIN ISOLATE OF RICE FLOUR: CHARACTERIZATION E FUNCTIONAL PROPERTIES
author Latorres, Juliana Machado
author_facet Latorres, Juliana Machado
Paiva, Ana Raisa
Gonzales, Bruna Araújo
Mellado, Myriam Salas
author_role author
author2 Paiva, Ana Raisa
Gonzales, Bruna Araújo
Mellado, Myriam Salas
author2_role author
author
author
dc.contributor.author.fl_str_mv Latorres, Juliana Machado
Paiva, Ana Raisa
Gonzales, Bruna Araújo
Mellado, Myriam Salas
dc.subject.por.fl_str_mv Engenharias
Ciências Agrárias
Alimentos
topic Engenharias
Ciências Agrárias
Alimentos
description The aim of this study was to isolate proteins from rice flour using an enzymatic method with the enzyme Termamyl®. The functional properties of solubility, water and oil holding capacity, emulsifying capacity, foam property and "in vitro" digestibility in flour and isolate were determined. The protein isolate was obtained after the reaction with the enzyme Termamyl® during 90 minutes at 90 ºC. The slurry was then filtered and dried at 35 ºC during 24 hours, obtaining 91.2 % of protein (dry basis) in the isolate. The values of the functional properties presented by the rice flour were: 17.8 % solubility at pH 10 and 6.2 % at pH 5; 1.13 mL oil/g of protein of emulsifying capacity; 2.46 g/g of sample of water holding capacity; oil holding capacity of 1.61 g/g of sample, and "in vitro" digestibility of 57.6 %. The protein isolate presented the following values: solubility of 7.4 % at pH 11 and 1.1 % at pH of 4.5; emulsifying capacity of 3 mL oil/g of protein, water holding capacity of 5.4 g/g of sample and oil holding capacity of 6.3 g/g of sample and "in vitro" digestibility of 83.6 %. All the isolate functional properties values, except the solubility, were better than the raw material values.
publishDate 2016
dc.date.none.fl_str_mv 2016-09-22
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.furg.br/vetor/article/view/2240
url https://periodicos.furg.br/vetor/article/view/2240
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.furg.br/vetor/article/view/2240/3803
dc.rights.driver.fl_str_mv Copyright (c) 2016 VETOR - Revista de Ciências Exatas e Engenharias
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2016 VETOR - Revista de Ciências Exatas e Engenharias
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal do Rio Grande
publisher.none.fl_str_mv Universidade Federal do Rio Grande
dc.source.none.fl_str_mv VETOR - Journal of Exact Sciences and Engineering; Vol. 24 No. 1 (2014); 53-65
VETOR - Revista de Ciências Exatas e Engenharias; v. 24 n. 1 (2014); 53-65
2358-3452
0102-7352
reponame:Vetor (Online)
instname:Universidade Federal do Rio Grande (FURG)
instacron:FURG
instname_str Universidade Federal do Rio Grande (FURG)
instacron_str FURG
institution FURG
reponame_str Vetor (Online)
collection Vetor (Online)
repository.name.fl_str_mv Vetor (Online) - Universidade Federal do Rio Grande (FURG)
repository.mail.fl_str_mv gmplatt@furg.br
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