The laboratory as a tool to qualify tuberculosis diagnosis

Detalhes bibliográficos
Autor(a) principal: Honscha, Gunther
Data de Publicação: 2008
Outros Autores: Groll, Andrea Von, Valença, Mariana Soares, Soares, Daniela Fernandes Ramos, Sanchotene, Karine Ortiz, Scaini, Carlos James, Ribeiro, Marta Osório, Silva, Pedro Eduardo Almeida da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FURG (RI FURG)
Texto Completo: http://repositorio.furg.br/handle/1/2594
Resumo: OBJECTIVES: To evaluate the performance of laboratory diagnosis of tuberculosis, clinical samples underwent culture, species identification and drug susceptibility testing (DST). METHODS: A total of 554 samples from 269 patients were tested for smear microscopy using Kinyoun stain. Culture was performed in Ogawa-Kudoh medium and species identification was performed using the IS6110 amplified region. DST for rifampicin, isoniazid (INH) and streptomycin were carried out using the Resazurin assay. RESULTS: Cultures augmented the number of cases diagnosed by 22.1%, IS6110 amplification identified all Mycobacterium tuberculosis strains thus isolated and DST detected three strains resistant to INH and one multidrug-resistant strain. CONCLUSION: Simultaneous use of different techniques enhanced culture yield, species identification and detection of drug resistance even in a laboratory with limited facilities.
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spelling The laboratory as a tool to qualify tuberculosis diagnosisTuberculosisCultureMycobacterium tuberculosisDiagnosisOBJECTIVES: To evaluate the performance of laboratory diagnosis of tuberculosis, clinical samples underwent culture, species identification and drug susceptibility testing (DST). METHODS: A total of 554 samples from 269 patients were tested for smear microscopy using Kinyoun stain. Culture was performed in Ogawa-Kudoh medium and species identification was performed using the IS6110 amplified region. DST for rifampicin, isoniazid (INH) and streptomycin were carried out using the Resazurin assay. RESULTS: Cultures augmented the number of cases diagnosed by 22.1%, IS6110 amplification identified all Mycobacterium tuberculosis strains thus isolated and DST detected three strains resistant to INH and one multidrug-resistant strain. CONCLUSION: Simultaneous use of different techniques enhanced culture yield, species identification and detection of drug resistance even in a laboratory with limited facilities.OBJECTIFS : Pour évaluer les performances du diagnostic de laboratoire de la tuberculose, des échantillons cliniques ont été soumis à la culture, à l’identification de l’espèce et aux tests de sensibilité (DST). MÉTHODES : 554 échantillons provenant de 269 patients ont été testés par examen microscopique des frottis après coloration de Kinyoun. Les cultures ont été faites sur milieu d’Ogawa-Kudoh et l’identification des espèces a utilisé la région amplifiée IS6110. Les tests de sensibilité à la rifampicine, l’isoniazide (INH) et la streptomycine ont été menés par la technique de Resazurine. RÉSULTATS : Les cultures ont augmenté le nombre de cas diagnostiqués de 22,1%, alors que l’amplification d’IS6110 a identifié chaque souche isolée de Mycobacterium tuberculosis et que les DST ont détecté trois souches résistantes à INH et une souche multirésistante. CONCLUSION : L’utilisation simultanée de différentes techniques a augmenté le rendement des cultures, l’identification des espèces et la détection de la résistance aux médicaments, même dans un laboratoire ayant des ressources limitées.OBJETIVOS : Con el propósito de evaluar la eficacia del diagnóstico de laboratorio de la tuberculosis, se sometió una serie de muestras clínicas a cultivo, identificación de especie y pruebas de sensibilidad a los medicamentos. MÉTODOS : Se analizaron 554 muestras provenientes de 269 pacientes mediante examen microscópico del esputo con coloración de Kinyoun, cultivos en medio OgawaKudoh e identificación de especies por amplificación de la región IS6110. Las pruebas de sensibilidad a rifampicina, isoniazida (INH) y estreptomicina se realizaron con el ensayo de azul de Alamar (Resazurin). RESULTADOS : Los cultivos aumentaron un 22,1% el número de casos diagnosticados y con la amplificación de la región IS6110 se identificaron todas las cepas de Mycobacterium tuberculosis así aisladas ; mediante las pruebas de sensibilidad se detectaron tres cepas resistentes a INH y una cepa multidrogorresistente. CONCLUSIÓN : La aplicación simultánea de diferentes técnicas aumentó la eficacia de los cultivos, la identificación de especies y las pruebas de farmacorresistencia, aún en un laboratorio con medios limitados.2012-09-26T23:41:17Z2012-09-26T23:41:17Z2008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfHONSCHA, Gunther et al. The laboratory as a tool to qualify tuberculosis diagnosis. The International Journal of Tuberculosis and Lung Disease, v. 12, n. 2, p. 218-220, 2008. Disponível em: <http://tbevidence.org/documents/rescentre/sop/studies/Ogawa-Kudoh%20method%20for%20TB/3.pdf>. Acesso em: 18 set. 2012.http://repositorio.furg.br/handle/1/2594engHonscha, GuntherGroll, Andrea VonValença, Mariana SoaresSoares, Daniela Fernandes RamosSanchotene, Karine OrtizScaini, Carlos JamesRibeiro, Marta OsórioSilva, Pedro Eduardo Almeida dainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURG2019-12-04T01:16:59Zoai:repositorio.furg.br:1/2594Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2019-12-04T01:16:59Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false
dc.title.none.fl_str_mv The laboratory as a tool to qualify tuberculosis diagnosis
title The laboratory as a tool to qualify tuberculosis diagnosis
spellingShingle The laboratory as a tool to qualify tuberculosis diagnosis
Honscha, Gunther
Tuberculosis
Culture
Mycobacterium tuberculosis
Diagnosis
title_short The laboratory as a tool to qualify tuberculosis diagnosis
title_full The laboratory as a tool to qualify tuberculosis diagnosis
title_fullStr The laboratory as a tool to qualify tuberculosis diagnosis
title_full_unstemmed The laboratory as a tool to qualify tuberculosis diagnosis
title_sort The laboratory as a tool to qualify tuberculosis diagnosis
author Honscha, Gunther
author_facet Honscha, Gunther
Groll, Andrea Von
Valença, Mariana Soares
Soares, Daniela Fernandes Ramos
Sanchotene, Karine Ortiz
Scaini, Carlos James
Ribeiro, Marta Osório
Silva, Pedro Eduardo Almeida da
author_role author
author2 Groll, Andrea Von
Valença, Mariana Soares
Soares, Daniela Fernandes Ramos
Sanchotene, Karine Ortiz
Scaini, Carlos James
Ribeiro, Marta Osório
Silva, Pedro Eduardo Almeida da
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Honscha, Gunther
Groll, Andrea Von
Valença, Mariana Soares
Soares, Daniela Fernandes Ramos
Sanchotene, Karine Ortiz
Scaini, Carlos James
Ribeiro, Marta Osório
Silva, Pedro Eduardo Almeida da
dc.subject.por.fl_str_mv Tuberculosis
Culture
Mycobacterium tuberculosis
Diagnosis
topic Tuberculosis
Culture
Mycobacterium tuberculosis
Diagnosis
description OBJECTIVES: To evaluate the performance of laboratory diagnosis of tuberculosis, clinical samples underwent culture, species identification and drug susceptibility testing (DST). METHODS: A total of 554 samples from 269 patients were tested for smear microscopy using Kinyoun stain. Culture was performed in Ogawa-Kudoh medium and species identification was performed using the IS6110 amplified region. DST for rifampicin, isoniazid (INH) and streptomycin were carried out using the Resazurin assay. RESULTS: Cultures augmented the number of cases diagnosed by 22.1%, IS6110 amplification identified all Mycobacterium tuberculosis strains thus isolated and DST detected three strains resistant to INH and one multidrug-resistant strain. CONCLUSION: Simultaneous use of different techniques enhanced culture yield, species identification and detection of drug resistance even in a laboratory with limited facilities.
publishDate 2008
dc.date.none.fl_str_mv 2008
2012-09-26T23:41:17Z
2012-09-26T23:41:17Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv HONSCHA, Gunther et al. The laboratory as a tool to qualify tuberculosis diagnosis. The International Journal of Tuberculosis and Lung Disease, v. 12, n. 2, p. 218-220, 2008. Disponível em: <http://tbevidence.org/documents/rescentre/sop/studies/Ogawa-Kudoh%20method%20for%20TB/3.pdf>. Acesso em: 18 set. 2012.
http://repositorio.furg.br/handle/1/2594
identifier_str_mv HONSCHA, Gunther et al. The laboratory as a tool to qualify tuberculosis diagnosis. The International Journal of Tuberculosis and Lung Disease, v. 12, n. 2, p. 218-220, 2008. Disponível em: <http://tbevidence.org/documents/rescentre/sop/studies/Ogawa-Kudoh%20method%20for%20TB/3.pdf>. Acesso em: 18 set. 2012.
url http://repositorio.furg.br/handle/1/2594
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
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instacron:FURG
instname_str Universidade Federal do Rio Grande (FURG)
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institution FURG
reponame_str Repositório Institucional da FURG (RI FURG)
collection Repositório Institucional da FURG (RI FURG)
repository.name.fl_str_mv Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)
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