Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building.

Detalhes bibliográficos
Autor(a) principal: David, S
Data de Publicação: 2012
Outros Autores: Sutre, A, Sanca, A, Mané, A, Henriques, V, Portugal, C, Sancho, L, Cardoso, A, Paixão, E, Duarte, E, Leite, C, Salem, J, Antunes, A
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.10/990
Resumo: The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair rpoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings.
id RCAP_157b20b1a949de23f4c075d555ac6605
oai_identifier_str oai:repositorio.hff.min-saude.pt:10400.10/990
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building.TuberculoseTuberculosisClinical laboratory techniquesMycobacterium tuberculosisThe diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair rpoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings.International Union against Tuberculosis and Lung DiseaseRepositório do Hospital Prof. Doutor Fernando FonsecaDavid, SSutre, ASanca, AMané, AHenriques, VPortugal, CSancho, LCardoso, APaixão, EDuarte, ELeite, CSalem, JAntunes, A2013-08-12T16:52:13Z2012-01-01T00:00:00Z2012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.10/990engInt J Tuberc Lung Dis. 2012 Nov;16(11):1535-71027-3719metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-20T15:51:48Zoai:repositorio.hff.min-saude.pt:10400.10/990Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:52:10.209842Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building.
title Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building.
spellingShingle Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building.
David, S
Tuberculose
Tuberculosis
Clinical laboratory techniques
Mycobacterium tuberculosis
title_short Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building.
title_full Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building.
title_fullStr Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building.
title_full_unstemmed Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building.
title_sort Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building.
author David, S
author_facet David, S
Sutre, A
Sanca, A
Mané, A
Henriques, V
Portugal, C
Sancho, L
Cardoso, A
Paixão, E
Duarte, E
Leite, C
Salem, J
Antunes, A
author_role author
author2 Sutre, A
Sanca, A
Mané, A
Henriques, V
Portugal, C
Sancho, L
Cardoso, A
Paixão, E
Duarte, E
Leite, C
Salem, J
Antunes, A
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório do Hospital Prof. Doutor Fernando Fonseca
dc.contributor.author.fl_str_mv David, S
Sutre, A
Sanca, A
Mané, A
Henriques, V
Portugal, C
Sancho, L
Cardoso, A
Paixão, E
Duarte, E
Leite, C
Salem, J
Antunes, A
dc.subject.por.fl_str_mv Tuberculose
Tuberculosis
Clinical laboratory techniques
Mycobacterium tuberculosis
topic Tuberculose
Tuberculosis
Clinical laboratory techniques
Mycobacterium tuberculosis
description The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair rpoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings.
publishDate 2012
dc.date.none.fl_str_mv 2012-01-01T00:00:00Z
2012-01-01T00:00:00Z
2013-08-12T16:52:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.10/990
url http://hdl.handle.net/10400.10/990
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Int J Tuberc Lung Dis. 2012 Nov;16(11):1535-7
1027-3719
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv International Union against Tuberculosis and Lung Disease
publisher.none.fl_str_mv International Union against Tuberculosis and Lung Disease
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1817551683263135744