Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building.
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.10/990 |
Resumo: | The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair rpoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
spelling |
Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building.TuberculoseTuberculosisClinical laboratory techniquesMycobacterium tuberculosisThe diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair rpoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings.International Union against Tuberculosis and Lung DiseaseRepositório do Hospital Prof. Doutor Fernando FonsecaDavid, SSutre, ASanca, AMané, AHenriques, VPortugal, CSancho, LCardoso, APaixão, EDuarte, ELeite, CSalem, JAntunes, A2013-08-12T16:52:13Z2012-01-01T00:00:00Z2012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.10/990engInt J Tuberc Lung Dis. 2012 Nov;16(11):1535-71027-3719metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-20T15:51:48Zoai:repositorio.hff.min-saude.pt:10400.10/990Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:52:10.209842Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building. |
title |
Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building. |
spellingShingle |
Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building. David, S Tuberculose Tuberculosis Clinical laboratory techniques Mycobacterium tuberculosis |
title_short |
Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building. |
title_full |
Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building. |
title_fullStr |
Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building. |
title_full_unstemmed |
Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building. |
title_sort |
Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building. |
author |
David, S |
author_facet |
David, S Sutre, A Sanca, A Mané, A Henriques, V Portugal, C Sancho, L Cardoso, A Paixão, E Duarte, E Leite, C Salem, J Antunes, A |
author_role |
author |
author2 |
Sutre, A Sanca, A Mané, A Henriques, V Portugal, C Sancho, L Cardoso, A Paixão, E Duarte, E Leite, C Salem, J Antunes, A |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório do Hospital Prof. Doutor Fernando Fonseca |
dc.contributor.author.fl_str_mv |
David, S Sutre, A Sanca, A Mané, A Henriques, V Portugal, C Sancho, L Cardoso, A Paixão, E Duarte, E Leite, C Salem, J Antunes, A |
dc.subject.por.fl_str_mv |
Tuberculose Tuberculosis Clinical laboratory techniques Mycobacterium tuberculosis |
topic |
Tuberculose Tuberculosis Clinical laboratory techniques Mycobacterium tuberculosis |
description |
The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair rpoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01T00:00:00Z 2012-01-01T00:00:00Z 2013-08-12T16:52:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.10/990 |
url |
http://hdl.handle.net/10400.10/990 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Int J Tuberc Lung Dis. 2012 Nov;16(11):1535-7 1027-3719 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
International Union against Tuberculosis and Lung Disease |
publisher.none.fl_str_mv |
International Union against Tuberculosis and Lung Disease |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1817551683263135744 |