Determination of IgG avidity in BALB/c mice experimentally infected with toxocara canis

Detalhes bibliográficos
Autor(a) principal: Schoenardie, Elizandra Roselaine
Data de Publicação: 2014
Outros Autores: Scaini, Carlos James, Avila, Luciana Farias da Costa de, Sperotto, Rita Leal, Borsuk, Sibele, Felicetti, Cristiane Pires Dias, Pepe, Michele, Berne, Maria Elisabeth Aires
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FURG (RI FURG)
Texto Completo: http://repositorio.furg.br/handle/1/7065
Resumo: Toxocariasis is a zoonotic disease in that IgM titers can remain high for long periods making difficult to determine the stage of the disease. The aim of this study is to investigate the applicability of indirect ELISA, associated with urea, to discriminate between the acute and chronic toxocariasis. IgG avidity was evaluated in 25 BALB/c mice experimentally infected with 1000 Toxocara canis eggs. Blood samples were collected, and sera treated with 6 M urea and assayed by ELISA every two weeks. The percent IgG avidity was determined using the mean absorbance of sera treated with urea, divided by the mean absorbance of untreated sera. In the first 15 days post-inoculation, was observed a low percentage, between 7.25 and 27.5%, IgG avidity, characteristic of an acute infection. After 60 days of infection, all the mice showed between 31.4 and 58% IgG avidity, indicating a chronic infection.
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spelling Determination of IgG avidity in BALB/c mice experimentally infected with toxocara canisDeterminação da avidez de IgG em camundongos BALB/c experimentalmente infectados com Toxocara canisVisceral larva migransToxocariasisAcute and chronic infectionsELISALarva migrans visceralToxocaríaseInfecção aguda e crônicaToxocariasis is a zoonotic disease in that IgM titers can remain high for long periods making difficult to determine the stage of the disease. The aim of this study is to investigate the applicability of indirect ELISA, associated with urea, to discriminate between the acute and chronic toxocariasis. IgG avidity was evaluated in 25 BALB/c mice experimentally infected with 1000 Toxocara canis eggs. Blood samples were collected, and sera treated with 6 M urea and assayed by ELISA every two weeks. The percent IgG avidity was determined using the mean absorbance of sera treated with urea, divided by the mean absorbance of untreated sera. In the first 15 days post-inoculation, was observed a low percentage, between 7.25 and 27.5%, IgG avidity, characteristic of an acute infection. After 60 days of infection, all the mice showed between 31.4 and 58% IgG avidity, indicating a chronic infection.A toxocaríase é uma zoonose na qual os títulos de IgM podem permanecer elevados por longos períodos, tornando difícil a determinação do estágio em que a doença se encontra. O objetivo deste estudo foi investigar a aplicabilidade de um teste indireto de ELISA, associado com ureia, para fazer a discriminação entre as fases aguda e crônica da toxocaríase. A avidez de IgG foi avaliada em 25 camundongos BALB/c experimentalmente infectados com 1000 ovos embrionados de Toxocara canis. A cada duas semanas, amostras de sangue foram coletadas, o soro tratado com ureia 6M e realizado o ensaio pela técnica de ELISA. O percentual de avidez de IgG foi determinado, usando-se a média das absorbâncias dos soros tratados com ureia dividida pela média das absorbâncias dos soros não tratados. Nos primeiros 15 dias pós-inoculação, foi observado um baixo percentual de avidez de IgG, entre 7,25 e 27,5%, característico da fase aguda da infecção. Após 60 dias de infecção, todos apresentaram avidez de IgG entre 31,4 e 58%, indicando a fase crônica da infecção.2017-02-11T00:20:16Z2017-02-11T00:20:16Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfSCHOENARDIE, Elizandra Roselaine et al. Determination of IgG avidity in BALB/c mice experimentally infected with toxocara canis. Revista Brasileira de Parasitologia Veterinária, v. 23, p. 403-406, 2014. Disponível em: <http://www.scielo.br/pdf/rbpv/v23n3/0103-846X-rbpv-23-03-403.pdf>. Acesso em: 04 fev. 2017.1984-2961http://repositorio.furg.br/handle/1/706510.1590/S1984-29612014060engSchoenardie, Elizandra RoselaineScaini, Carlos JamesAvila, Luciana Farias da Costa deSperotto, Rita LealBorsuk, SibeleFelicetti, Cristiane Pires DiasPepe, MicheleBerne, Maria Elisabeth Airesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURG2023-08-18T14:39:02Zoai:repositorio.furg.br:1/7065Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2023-08-18T14:39:02Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false
dc.title.none.fl_str_mv Determination of IgG avidity in BALB/c mice experimentally infected with toxocara canis
Determinação da avidez de IgG em camundongos BALB/c experimentalmente infectados com Toxocara canis
title Determination of IgG avidity in BALB/c mice experimentally infected with toxocara canis
spellingShingle Determination of IgG avidity in BALB/c mice experimentally infected with toxocara canis
Schoenardie, Elizandra Roselaine
Visceral larva migrans
Toxocariasis
Acute and chronic infections
ELISA
Larva migrans visceral
Toxocaríase
Infecção aguda e crônica
title_short Determination of IgG avidity in BALB/c mice experimentally infected with toxocara canis
title_full Determination of IgG avidity in BALB/c mice experimentally infected with toxocara canis
title_fullStr Determination of IgG avidity in BALB/c mice experimentally infected with toxocara canis
title_full_unstemmed Determination of IgG avidity in BALB/c mice experimentally infected with toxocara canis
title_sort Determination of IgG avidity in BALB/c mice experimentally infected with toxocara canis
author Schoenardie, Elizandra Roselaine
author_facet Schoenardie, Elizandra Roselaine
Scaini, Carlos James
Avila, Luciana Farias da Costa de
Sperotto, Rita Leal
Borsuk, Sibele
Felicetti, Cristiane Pires Dias
Pepe, Michele
Berne, Maria Elisabeth Aires
author_role author
author2 Scaini, Carlos James
Avila, Luciana Farias da Costa de
Sperotto, Rita Leal
Borsuk, Sibele
Felicetti, Cristiane Pires Dias
Pepe, Michele
Berne, Maria Elisabeth Aires
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Schoenardie, Elizandra Roselaine
Scaini, Carlos James
Avila, Luciana Farias da Costa de
Sperotto, Rita Leal
Borsuk, Sibele
Felicetti, Cristiane Pires Dias
Pepe, Michele
Berne, Maria Elisabeth Aires
dc.subject.por.fl_str_mv Visceral larva migrans
Toxocariasis
Acute and chronic infections
ELISA
Larva migrans visceral
Toxocaríase
Infecção aguda e crônica
topic Visceral larva migrans
Toxocariasis
Acute and chronic infections
ELISA
Larva migrans visceral
Toxocaríase
Infecção aguda e crônica
description Toxocariasis is a zoonotic disease in that IgM titers can remain high for long periods making difficult to determine the stage of the disease. The aim of this study is to investigate the applicability of indirect ELISA, associated with urea, to discriminate between the acute and chronic toxocariasis. IgG avidity was evaluated in 25 BALB/c mice experimentally infected with 1000 Toxocara canis eggs. Blood samples were collected, and sera treated with 6 M urea and assayed by ELISA every two weeks. The percent IgG avidity was determined using the mean absorbance of sera treated with urea, divided by the mean absorbance of untreated sera. In the first 15 days post-inoculation, was observed a low percentage, between 7.25 and 27.5%, IgG avidity, characteristic of an acute infection. After 60 days of infection, all the mice showed between 31.4 and 58% IgG avidity, indicating a chronic infection.
publishDate 2014
dc.date.none.fl_str_mv 2014
2017-02-11T00:20:16Z
2017-02-11T00:20:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv SCHOENARDIE, Elizandra Roselaine et al. Determination of IgG avidity in BALB/c mice experimentally infected with toxocara canis. Revista Brasileira de Parasitologia Veterinária, v. 23, p. 403-406, 2014. Disponível em: <http://www.scielo.br/pdf/rbpv/v23n3/0103-846X-rbpv-23-03-403.pdf>. Acesso em: 04 fev. 2017.
1984-2961
http://repositorio.furg.br/handle/1/7065
10.1590/S1984-29612014060
identifier_str_mv SCHOENARDIE, Elizandra Roselaine et al. Determination of IgG avidity in BALB/c mice experimentally infected with toxocara canis. Revista Brasileira de Parasitologia Veterinária, v. 23, p. 403-406, 2014. Disponível em: <http://www.scielo.br/pdf/rbpv/v23n3/0103-846X-rbpv-23-03-403.pdf>. Acesso em: 04 fev. 2017.
1984-2961
10.1590/S1984-29612014060
url http://repositorio.furg.br/handle/1/7065
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da FURG (RI FURG)
instname:Universidade Federal do Rio Grande (FURG)
instacron:FURG
instname_str Universidade Federal do Rio Grande (FURG)
instacron_str FURG
institution FURG
reponame_str Repositório Institucional da FURG (RI FURG)
collection Repositório Institucional da FURG (RI FURG)
repository.name.fl_str_mv Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)
repository.mail.fl_str_mv
_version_ 1822808178139594752

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