Determination of IgG avidity in BALB/c mice experimentally infected with Toxocara canis

Detalhes bibliográficos
Autor(a) principal: Schoenardie,Elizandra Roselaine
Data de Publicação: 2014
Outros Autores: Scaini,Carlos James, Avila,Luciana Farias da Costa de, Sperotto,Rita Leal, Borsuk,Sibele, Felicetti,Cristine Dias Pires, Pepe,Michele, Berne,Maria Elisabeth Aires
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Parasitologia Veterinária (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612014000300403
Resumo: Toxocariasis is a zoonotic disease in that IgM titers can remain high for long periods making difficult to determine the stage of the disease. The aim of this study is to investigate the applicability of indirect ELISA, associated with urea, to discriminate between the acute and chronic toxocariasis. IgG avidity was evaluated in 25 BALB/c mice experimentally infected with 1000 Toxocara canis eggs. Blood samples were collected, and sera treated with 6 M urea and assayed by ELISA every two weeks. The percent IgG avidity was determined using the mean absorbance of sera treated with urea, divided by the mean absorbance of untreated sera. In the first 15 days post-inoculation, was observed a low percentage, between 7.25 and 27.5%, IgG avidity, characteristic of an acute infection. After 60 days of infection, all the mice showed between 31.4 and 58% IgG avidity, indicating a chronic infection.
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spelling Determination of IgG avidity in BALB/c mice experimentally infected with Toxocara canisVisceral larva migranstoxocariasisacute and chronic infectionsELISAToxocariasis is a zoonotic disease in that IgM titers can remain high for long periods making difficult to determine the stage of the disease. The aim of this study is to investigate the applicability of indirect ELISA, associated with urea, to discriminate between the acute and chronic toxocariasis. IgG avidity was evaluated in 25 BALB/c mice experimentally infected with 1000 Toxocara canis eggs. Blood samples were collected, and sera treated with 6 M urea and assayed by ELISA every two weeks. The percent IgG avidity was determined using the mean absorbance of sera treated with urea, divided by the mean absorbance of untreated sera. In the first 15 days post-inoculation, was observed a low percentage, between 7.25 and 27.5%, IgG avidity, characteristic of an acute infection. After 60 days of infection, all the mice showed between 31.4 and 58% IgG avidity, indicating a chronic infection.Colégio Brasileiro de Parasitologia Veterinária2014-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612014000300403Revista Brasileira de Parasitologia Veterinária v.23 n.3 2014reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/S1984-29612014060info:eu-repo/semantics/openAccessSchoenardie,Elizandra RoselaineScaini,Carlos JamesAvila,Luciana Farias da Costa deSperotto,Rita LealBorsuk,SibeleFelicetti,Cristine Dias PiresPepe,MicheleBerne,Maria Elisabeth Aireseng2015-09-23T00:00:00Zoai:scielo:S1984-29612014000300403Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2015-09-23T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false
dc.title.none.fl_str_mv Determination of IgG avidity in BALB/c mice experimentally infected with Toxocara canis
title Determination of IgG avidity in BALB/c mice experimentally infected with Toxocara canis
spellingShingle Determination of IgG avidity in BALB/c mice experimentally infected with Toxocara canis
Schoenardie,Elizandra Roselaine
Visceral larva migrans
toxocariasis
acute and chronic infections
ELISA
title_short Determination of IgG avidity in BALB/c mice experimentally infected with Toxocara canis
title_full Determination of IgG avidity in BALB/c mice experimentally infected with Toxocara canis
title_fullStr Determination of IgG avidity in BALB/c mice experimentally infected with Toxocara canis
title_full_unstemmed Determination of IgG avidity in BALB/c mice experimentally infected with Toxocara canis
title_sort Determination of IgG avidity in BALB/c mice experimentally infected with Toxocara canis
author Schoenardie,Elizandra Roselaine
author_facet Schoenardie,Elizandra Roselaine
Scaini,Carlos James
Avila,Luciana Farias da Costa de
Sperotto,Rita Leal
Borsuk,Sibele
Felicetti,Cristine Dias Pires
Pepe,Michele
Berne,Maria Elisabeth Aires
author_role author
author2 Scaini,Carlos James
Avila,Luciana Farias da Costa de
Sperotto,Rita Leal
Borsuk,Sibele
Felicetti,Cristine Dias Pires
Pepe,Michele
Berne,Maria Elisabeth Aires
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Schoenardie,Elizandra Roselaine
Scaini,Carlos James
Avila,Luciana Farias da Costa de
Sperotto,Rita Leal
Borsuk,Sibele
Felicetti,Cristine Dias Pires
Pepe,Michele
Berne,Maria Elisabeth Aires
dc.subject.por.fl_str_mv Visceral larva migrans
toxocariasis
acute and chronic infections
ELISA
topic Visceral larva migrans
toxocariasis
acute and chronic infections
ELISA
description Toxocariasis is a zoonotic disease in that IgM titers can remain high for long periods making difficult to determine the stage of the disease. The aim of this study is to investigate the applicability of indirect ELISA, associated with urea, to discriminate between the acute and chronic toxocariasis. IgG avidity was evaluated in 25 BALB/c mice experimentally infected with 1000 Toxocara canis eggs. Blood samples were collected, and sera treated with 6 M urea and assayed by ELISA every two weeks. The percent IgG avidity was determined using the mean absorbance of sera treated with urea, divided by the mean absorbance of untreated sera. In the first 15 days post-inoculation, was observed a low percentage, between 7.25 and 27.5%, IgG avidity, characteristic of an acute infection. After 60 days of infection, all the mice showed between 31.4 and 58% IgG avidity, indicating a chronic infection.
publishDate 2014
dc.date.none.fl_str_mv 2014-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612014000300403
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612014000300403
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1984-29612014060
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
dc.source.none.fl_str_mv Revista Brasileira de Parasitologia Veterinária v.23 n.3 2014
reponame:Revista Brasileira de Parasitologia Veterinária (Online)
instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)
instacron:CBPV
instname_str Colégio Brasileiro de Parasitologia Veterinária (CBPV)
instacron_str CBPV
institution CBPV
reponame_str Revista Brasileira de Parasitologia Veterinária (Online)
collection Revista Brasileira de Parasitologia Veterinária (Online)
repository.name.fl_str_mv Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)
repository.mail.fl_str_mv ||zacariascbpv@fcav.unesp.br
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