Investigation of copper binding to hydrosoluble proteins of an amphipod

Detalhes bibliográficos
Autor(a) principal: Pinho, Grasiela Lopes Leães
Data de Publicação: 2009
Outros Autores: Saint-Louis, Richard, Bianchini, Adalto, Pelletier, Émilien
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FURG (RI FURG)
Texto Completo: http://repositorio.furg.br/handle/1/1935
Resumo: This study investigated the body copper accumulation in the amphipod Gammarus sp.. Groups of copper-binding proteins were also investigated considering their molecular weights. Regarding copper accumulation, a saturation type kinetics was observed with Km and Vmax values of 0.48 mg Cu.L–1 and 713.3 μg Cu.g–1 dw, respectively. Size exclusion chromatography (5 to 150 KDa) associated with ICP-MS showed that most of copper-binding proteins in Gammarus sp. have molecular weights <12 KDa, probably corresponding to metallothioneins.
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spelling Pinho, Grasiela Lopes LeãesSaint-Louis, RichardBianchini, AdaltoPelletier, Émilien2012-03-17T05:03:40Z2012-03-17T05:03:40Z2009PINHO, Grasiela Lopes Leães et al. Investigation of copper binding to hydrosoluble proteins of an amphipod. Journal Of The Brazilian Society Of Ecotoxicology, v. 4, n. 1-3, p. 83-86, 2009. Disponível em: <http://ecotoxbrasil.org.br/index.php?option=com_rokdownloads&view=folder&Itemid=53&limitstart=10>. Acesso em: 07 mar. 2012.1809-8401http://repositorio.furg.br/handle/1/1935This study investigated the body copper accumulation in the amphipod Gammarus sp.. Groups of copper-binding proteins were also investigated considering their molecular weights. Regarding copper accumulation, a saturation type kinetics was observed with Km and Vmax values of 0.48 mg Cu.L–1 and 713.3 μg Cu.g–1 dw, respectively. Size exclusion chromatography (5 to 150 KDa) associated with ICP-MS showed that most of copper-binding proteins in Gammarus sp. have molecular weights <12 KDa, probably corresponding to metallothioneins.Este estudo investigou a acumulação corporal do cobre no anfípodo Gammarus sp.. Grupos de proteínas ligadas ao cobre foram também investigadas considerando seu peso molecular. Em relação à acumulação de cobre, foi observada uma sinética do tipo saturação com valores de Km e Vmax de 0,48 mg Cu.L–1 e 713,3 μg Cu.g–1 ps, respectivamente. A cromatografia de exclusão por tamanho (5 a 150 KDa) associada ao ICP-MS, mostrou que a maioria das proteínas ligadas ao cobre em Gammarus sp. têm peso molecular <12 KDa, provavelmente correspondendo às metalotioneínas.engCopper accumulationGammarus sp.ICPMSMetallothioneinsSize exclusion chromatographyAcumulação de cobreCromatografia de exclusão por tamanhoMetalotioneínasInvestigation of copper binding to hydrosoluble proteins of an amphipodInvestigação da ligação do cobre às proteínas hidrossolúveis de um anfípodoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURGORIGINALInvestigation of Copper Binding to.pdfInvestigation of Copper Binding to.pdfapplication/pdf1009329https://repositorio.furg.br/bitstream/1/1935/1/Investigation%20of%20Copper%20Binding%20to.pdfc4854f75b053aacc58f64e121f302cdeMD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-81724https://repositorio.furg.br/bitstream/1/1935/2/license.txt5b92b9704b4f13242d70e45ddef35a68MD52open access1/19352012-03-17 02:03:40.542open accessoai:repositorio.furg.br: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Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2012-03-17T05:03:40Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false
dc.title.pt_BR.fl_str_mv Investigation of copper binding to hydrosoluble proteins of an amphipod
dc.title.alternative.pt_BR.fl_str_mv Investigação da ligação do cobre às proteínas hidrossolúveis de um anfípodo
title Investigation of copper binding to hydrosoluble proteins of an amphipod
spellingShingle Investigation of copper binding to hydrosoluble proteins of an amphipod
Pinho, Grasiela Lopes Leães
Copper accumulation
Gammarus sp.
ICPMS
Metallothioneins
Size exclusion chromatography
Acumulação de cobre
Cromatografia de exclusão por tamanho
Metalotioneínas
title_short Investigation of copper binding to hydrosoluble proteins of an amphipod
title_full Investigation of copper binding to hydrosoluble proteins of an amphipod
title_fullStr Investigation of copper binding to hydrosoluble proteins of an amphipod
title_full_unstemmed Investigation of copper binding to hydrosoluble proteins of an amphipod
title_sort Investigation of copper binding to hydrosoluble proteins of an amphipod
author Pinho, Grasiela Lopes Leães
author_facet Pinho, Grasiela Lopes Leães
Saint-Louis, Richard
Bianchini, Adalto
Pelletier, Émilien
author_role author
author2 Saint-Louis, Richard
Bianchini, Adalto
Pelletier, Émilien
author2_role author
author
author
dc.contributor.author.fl_str_mv Pinho, Grasiela Lopes Leães
Saint-Louis, Richard
Bianchini, Adalto
Pelletier, Émilien
dc.subject.por.fl_str_mv Copper accumulation
Gammarus sp.
ICPMS
Metallothioneins
Size exclusion chromatography
Acumulação de cobre
Cromatografia de exclusão por tamanho
Metalotioneínas
topic Copper accumulation
Gammarus sp.
ICPMS
Metallothioneins
Size exclusion chromatography
Acumulação de cobre
Cromatografia de exclusão por tamanho
Metalotioneínas
description This study investigated the body copper accumulation in the amphipod Gammarus sp.. Groups of copper-binding proteins were also investigated considering their molecular weights. Regarding copper accumulation, a saturation type kinetics was observed with Km and Vmax values of 0.48 mg Cu.L–1 and 713.3 μg Cu.g–1 dw, respectively. Size exclusion chromatography (5 to 150 KDa) associated with ICP-MS showed that most of copper-binding proteins in Gammarus sp. have molecular weights <12 KDa, probably corresponding to metallothioneins.
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2012-03-17T05:03:40Z
dc.date.available.fl_str_mv 2012-03-17T05:03:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PINHO, Grasiela Lopes Leães et al. Investigation of copper binding to hydrosoluble proteins of an amphipod. Journal Of The Brazilian Society Of Ecotoxicology, v. 4, n. 1-3, p. 83-86, 2009. Disponível em: <http://ecotoxbrasil.org.br/index.php?option=com_rokdownloads&view=folder&Itemid=53&limitstart=10>. Acesso em: 07 mar. 2012.
dc.identifier.uri.fl_str_mv http://repositorio.furg.br/handle/1/1935
dc.identifier.issn.none.fl_str_mv 1809-8401
identifier_str_mv PINHO, Grasiela Lopes Leães et al. Investigation of copper binding to hydrosoluble proteins of an amphipod. Journal Of The Brazilian Society Of Ecotoxicology, v. 4, n. 1-3, p. 83-86, 2009. Disponível em: <http://ecotoxbrasil.org.br/index.php?option=com_rokdownloads&view=folder&Itemid=53&limitstart=10>. Acesso em: 07 mar. 2012.
1809-8401
url http://repositorio.furg.br/handle/1/1935
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FURG (RI FURG)
instname:Universidade Federal do Rio Grande (FURG)
instacron:FURG
instname_str Universidade Federal do Rio Grande (FURG)
instacron_str FURG
institution FURG
reponame_str Repositório Institucional da FURG (RI FURG)
collection Repositório Institucional da FURG (RI FURG)
bitstream.url.fl_str_mv https://repositorio.furg.br/bitstream/1/1935/1/Investigation%20of%20Copper%20Binding%20to.pdf
https://repositorio.furg.br/bitstream/1/1935/2/license.txt
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