Investigation of copper binding to hydrosoluble proteins of an amphipod
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FURG (RI FURG) |
Texto Completo: | http://repositorio.furg.br/handle/1/1935 |
Resumo: | This study investigated the body copper accumulation in the amphipod Gammarus sp.. Groups of copper-binding proteins were also investigated considering their molecular weights. Regarding copper accumulation, a saturation type kinetics was observed with Km and Vmax values of 0.48 mg Cu.L–1 and 713.3 μg Cu.g–1 dw, respectively. Size exclusion chromatography (5 to 150 KDa) associated with ICP-MS showed that most of copper-binding proteins in Gammarus sp. have molecular weights <12 KDa, probably corresponding to metallothioneins. |
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Investigation of copper binding to hydrosoluble proteins of an amphipodInvestigação da ligação do cobre às proteínas hidrossolúveis de um anfípodoCopper accumulationGammarus sp.ICPMSMetallothioneinsSize exclusion chromatographyAcumulação de cobreCromatografia de exclusão por tamanhoMetalotioneínasThis study investigated the body copper accumulation in the amphipod Gammarus sp.. Groups of copper-binding proteins were also investigated considering their molecular weights. Regarding copper accumulation, a saturation type kinetics was observed with Km and Vmax values of 0.48 mg Cu.L–1 and 713.3 μg Cu.g–1 dw, respectively. Size exclusion chromatography (5 to 150 KDa) associated with ICP-MS showed that most of copper-binding proteins in Gammarus sp. have molecular weights <12 KDa, probably corresponding to metallothioneins.Este estudo investigou a acumulação corporal do cobre no anfípodo Gammarus sp.. Grupos de proteínas ligadas ao cobre foram também investigadas considerando seu peso molecular. Em relação à acumulação de cobre, foi observada uma sinética do tipo saturação com valores de Km e Vmax de 0,48 mg Cu.L–1 e 713,3 μg Cu.g–1 ps, respectivamente. A cromatografia de exclusão por tamanho (5 a 150 KDa) associada ao ICP-MS, mostrou que a maioria das proteínas ligadas ao cobre em Gammarus sp. têm peso molecular <12 KDa, provavelmente correspondendo às metalotioneínas.2012-03-17T05:03:40Z2012-03-17T05:03:40Z2009info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfPINHO, Grasiela Lopes Leães et al. Investigation of copper binding to hydrosoluble proteins of an amphipod. Journal Of The Brazilian Society Of Ecotoxicology, v. 4, n. 1-3, p. 83-86, 2009. Disponível em: <http://ecotoxbrasil.org.br/index.php?option=com_rokdownloads&view=folder&Itemid=53&limitstart=10>. Acesso em: 07 mar. 2012.1809-8401http://repositorio.furg.br/handle/1/1935engPinho, Grasiela Lopes LeãesSaint-Louis, RichardBianchini, AdaltoPelletier, Émilieninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURG2012-03-17T05:03:40Zoai:repositorio.furg.br:1/1935Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2012-03-17T05:03:40Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false |
dc.title.none.fl_str_mv |
Investigation of copper binding to hydrosoluble proteins of an amphipod Investigação da ligação do cobre às proteínas hidrossolúveis de um anfípodo |
title |
Investigation of copper binding to hydrosoluble proteins of an amphipod |
spellingShingle |
Investigation of copper binding to hydrosoluble proteins of an amphipod Pinho, Grasiela Lopes Leães Copper accumulation Gammarus sp. ICPMS Metallothioneins Size exclusion chromatography Acumulação de cobre Cromatografia de exclusão por tamanho Metalotioneínas |
title_short |
Investigation of copper binding to hydrosoluble proteins of an amphipod |
title_full |
Investigation of copper binding to hydrosoluble proteins of an amphipod |
title_fullStr |
Investigation of copper binding to hydrosoluble proteins of an amphipod |
title_full_unstemmed |
Investigation of copper binding to hydrosoluble proteins of an amphipod |
title_sort |
Investigation of copper binding to hydrosoluble proteins of an amphipod |
author |
Pinho, Grasiela Lopes Leães |
author_facet |
Pinho, Grasiela Lopes Leães Saint-Louis, Richard Bianchini, Adalto Pelletier, Émilien |
author_role |
author |
author2 |
Saint-Louis, Richard Bianchini, Adalto Pelletier, Émilien |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Pinho, Grasiela Lopes Leães Saint-Louis, Richard Bianchini, Adalto Pelletier, Émilien |
dc.subject.por.fl_str_mv |
Copper accumulation Gammarus sp. ICPMS Metallothioneins Size exclusion chromatography Acumulação de cobre Cromatografia de exclusão por tamanho Metalotioneínas |
topic |
Copper accumulation Gammarus sp. ICPMS Metallothioneins Size exclusion chromatography Acumulação de cobre Cromatografia de exclusão por tamanho Metalotioneínas |
description |
This study investigated the body copper accumulation in the amphipod Gammarus sp.. Groups of copper-binding proteins were also investigated considering their molecular weights. Regarding copper accumulation, a saturation type kinetics was observed with Km and Vmax values of 0.48 mg Cu.L–1 and 713.3 μg Cu.g–1 dw, respectively. Size exclusion chromatography (5 to 150 KDa) associated with ICP-MS showed that most of copper-binding proteins in Gammarus sp. have molecular weights <12 KDa, probably corresponding to metallothioneins. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009 2012-03-17T05:03:40Z 2012-03-17T05:03:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
PINHO, Grasiela Lopes Leães et al. Investigation of copper binding to hydrosoluble proteins of an amphipod. Journal Of The Brazilian Society Of Ecotoxicology, v. 4, n. 1-3, p. 83-86, 2009. Disponível em: <http://ecotoxbrasil.org.br/index.php?option=com_rokdownloads&view=folder&Itemid=53&limitstart=10>. Acesso em: 07 mar. 2012. 1809-8401 http://repositorio.furg.br/handle/1/1935 |
identifier_str_mv |
PINHO, Grasiela Lopes Leães et al. Investigation of copper binding to hydrosoluble proteins of an amphipod. Journal Of The Brazilian Society Of Ecotoxicology, v. 4, n. 1-3, p. 83-86, 2009. Disponível em: <http://ecotoxbrasil.org.br/index.php?option=com_rokdownloads&view=folder&Itemid=53&limitstart=10>. Acesso em: 07 mar. 2012. 1809-8401 |
url |
http://repositorio.furg.br/handle/1/1935 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FURG (RI FURG) instname:Universidade Federal do Rio Grande (FURG) instacron:FURG |
instname_str |
Universidade Federal do Rio Grande (FURG) |
instacron_str |
FURG |
institution |
FURG |
reponame_str |
Repositório Institucional da FURG (RI FURG) |
collection |
Repositório Institucional da FURG (RI FURG) |
repository.name.fl_str_mv |
Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG) |
repository.mail.fl_str_mv |
|
_version_ |
1807384393755394048 |