Evaluation of the Analgesic Effect of Aqueous Extract of Brugmansia suaveolens Flower in Mice: Possible Mechanism Involved
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FURG (RI FURG) |
Texto Completo: | http://repositorio.furg.br/handle/1/430 |
Resumo: | The study was conducted to test the aqueous extract of Brugmansia suaveolens (AEBs) flowers for their antinociceptive effects in mice. In the hot plate test, a significant increase in reaction time for two doses of AEBs at 60, 90, 120, and 150 min after treatment was noted. Pretreatment of animals with naloxone (5 mg/kg, intraperitoneally [IP]) left the antinociceptive effect of AEBs at a dose of 100 mg/kg unaffected at 60, 90, 120, and 150 min after treatment and at a dose of 300 mg/kg at 30 min but not at 90, 120, and 150 min. In the writhing test, the AEBs significantly inhibited acetic acid–induced abdominal constriction and was equally potent at both doses. Pretreatment with naloxone (5 mg/kg, IP) left the antinociceptive effect of both doses of AEBs unaffected. Pretreatment with NG-nitro-L-arginine methyl ester (L-NAME; 20 mg/kg, IP) caused a significant change in the number of abdominal constrictions but did not change the antinociceptive effect of AEBs. Pretreatment of animals with methylene blue also did not change the effect of AEBs on the number of writhing movements in mice. Flumazenil (5 mg/kg, IP) antagonized the antinociceptive effects of diazepam and also reversed the antinociceptive effect of AEBs. AEBs showed a depressant effect on the central nervous system, and the treatment of mice with pentobarbital combined with AEBs increased the animals’ sleeping time in a dose-dependent manner. These results suggest that the antinociceptive activity of AEBs may be related in part to benzodiazepine receptors, although peripheral mechanisms cannot be excluded |
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Baisch, Ana Luiza MuccilloParker, Alexander GarciaCardoso, Gianni PerazaVaz, Marta Regina CezarSoares, Maria Cristina Flores2011-04-14T23:11:33Z2011-04-14T23:11:33Z2010BAISCH, Ana Luiza Muccillo et al. Evaluation of the Analgesic Effect of Aqueous Extract of Brugmansia suaveolens Flower in Mice: Possible Mechanism Involved. Biological Research for Nursing. v. 11, n. 4, p. 345-350, 2010. Disponível em: <http://brn.sagepub.com/content/11/4/345>. Acesso em: 07 abr. 2011.1552-4175http://repositorio.furg.br/handle/1/430The study was conducted to test the aqueous extract of Brugmansia suaveolens (AEBs) flowers for their antinociceptive effects in mice. In the hot plate test, a significant increase in reaction time for two doses of AEBs at 60, 90, 120, and 150 min after treatment was noted. Pretreatment of animals with naloxone (5 mg/kg, intraperitoneally [IP]) left the antinociceptive effect of AEBs at a dose of 100 mg/kg unaffected at 60, 90, 120, and 150 min after treatment and at a dose of 300 mg/kg at 30 min but not at 90, 120, and 150 min. In the writhing test, the AEBs significantly inhibited acetic acid–induced abdominal constriction and was equally potent at both doses. Pretreatment with naloxone (5 mg/kg, IP) left the antinociceptive effect of both doses of AEBs unaffected. Pretreatment with NG-nitro-L-arginine methyl ester (L-NAME; 20 mg/kg, IP) caused a significant change in the number of abdominal constrictions but did not change the antinociceptive effect of AEBs. Pretreatment of animals with methylene blue also did not change the effect of AEBs on the number of writhing movements in mice. Flumazenil (5 mg/kg, IP) antagonized the antinociceptive effects of diazepam and also reversed the antinociceptive effect of AEBs. AEBs showed a depressant effect on the central nervous system, and the treatment of mice with pentobarbital combined with AEBs increased the animals’ sleeping time in a dose-dependent manner. These results suggest that the antinociceptive activity of AEBs may be related in part to benzodiazepine receptors, although peripheral mechanisms cannot be excludedengBrugmansia suaveolensPain measurementHot plate testAbdominal constrictionSleep testEvaluation of the Analgesic Effect of Aqueous Extract of Brugmansia suaveolens Flower in Mice: Possible Mechanism Involvedinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURGORIGINAL"Evaluation of the Analgesic Effect of Aqueous"Evaluation of the Analgesic Effect of Aqueousapplication/pdf158924https://repositorio.furg.br/bitstream/1/430/1/%22Evaluation%20of%20the%20Analgesic%20Effect%20of%20Aqueousa0596cfaba2104174eeae854559efe7aMD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-81724https://repositorio.furg.br/bitstream/1/430/2/license.txt5b92b9704b4f13242d70e45ddef35a68MD52open access1/4302011-10-31 14:50:17.942open accessoai:repositorio.furg.br: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Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2011-10-31T16:50:17Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false |
dc.title.pt_BR.fl_str_mv |
Evaluation of the Analgesic Effect of Aqueous Extract of Brugmansia suaveolens Flower in Mice: Possible Mechanism Involved |
title |
Evaluation of the Analgesic Effect of Aqueous Extract of Brugmansia suaveolens Flower in Mice: Possible Mechanism Involved |
spellingShingle |
Evaluation of the Analgesic Effect of Aqueous Extract of Brugmansia suaveolens Flower in Mice: Possible Mechanism Involved Baisch, Ana Luiza Muccillo Brugmansia suaveolens Pain measurement Hot plate test Abdominal constriction Sleep test |
title_short |
Evaluation of the Analgesic Effect of Aqueous Extract of Brugmansia suaveolens Flower in Mice: Possible Mechanism Involved |
title_full |
Evaluation of the Analgesic Effect of Aqueous Extract of Brugmansia suaveolens Flower in Mice: Possible Mechanism Involved |
title_fullStr |
Evaluation of the Analgesic Effect of Aqueous Extract of Brugmansia suaveolens Flower in Mice: Possible Mechanism Involved |
title_full_unstemmed |
Evaluation of the Analgesic Effect of Aqueous Extract of Brugmansia suaveolens Flower in Mice: Possible Mechanism Involved |
title_sort |
Evaluation of the Analgesic Effect of Aqueous Extract of Brugmansia suaveolens Flower in Mice: Possible Mechanism Involved |
author |
Baisch, Ana Luiza Muccillo |
author_facet |
Baisch, Ana Luiza Muccillo Parker, Alexander Garcia Cardoso, Gianni Peraza Vaz, Marta Regina Cezar Soares, Maria Cristina Flores |
author_role |
author |
author2 |
Parker, Alexander Garcia Cardoso, Gianni Peraza Vaz, Marta Regina Cezar Soares, Maria Cristina Flores |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Baisch, Ana Luiza Muccillo Parker, Alexander Garcia Cardoso, Gianni Peraza Vaz, Marta Regina Cezar Soares, Maria Cristina Flores |
dc.subject.por.fl_str_mv |
Brugmansia suaveolens Pain measurement Hot plate test Abdominal constriction Sleep test |
topic |
Brugmansia suaveolens Pain measurement Hot plate test Abdominal constriction Sleep test |
description |
The study was conducted to test the aqueous extract of Brugmansia suaveolens (AEBs) flowers for their antinociceptive effects in mice. In the hot plate test, a significant increase in reaction time for two doses of AEBs at 60, 90, 120, and 150 min after treatment was noted. Pretreatment of animals with naloxone (5 mg/kg, intraperitoneally [IP]) left the antinociceptive effect of AEBs at a dose of 100 mg/kg unaffected at 60, 90, 120, and 150 min after treatment and at a dose of 300 mg/kg at 30 min but not at 90, 120, and 150 min. In the writhing test, the AEBs significantly inhibited acetic acid–induced abdominal constriction and was equally potent at both doses. Pretreatment with naloxone (5 mg/kg, IP) left the antinociceptive effect of both doses of AEBs unaffected. Pretreatment with NG-nitro-L-arginine methyl ester (L-NAME; 20 mg/kg, IP) caused a significant change in the number of abdominal constrictions but did not change the antinociceptive effect of AEBs. Pretreatment of animals with methylene blue also did not change the effect of AEBs on the number of writhing movements in mice. Flumazenil (5 mg/kg, IP) antagonized the antinociceptive effects of diazepam and also reversed the antinociceptive effect of AEBs. AEBs showed a depressant effect on the central nervous system, and the treatment of mice with pentobarbital combined with AEBs increased the animals’ sleeping time in a dose-dependent manner. These results suggest that the antinociceptive activity of AEBs may be related in part to benzodiazepine receptors, although peripheral mechanisms cannot be excluded |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010 |
dc.date.accessioned.fl_str_mv |
2011-04-14T23:11:33Z |
dc.date.available.fl_str_mv |
2011-04-14T23:11:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BAISCH, Ana Luiza Muccillo et al. Evaluation of the Analgesic Effect of Aqueous Extract of Brugmansia suaveolens Flower in Mice: Possible Mechanism Involved. Biological Research for Nursing. v. 11, n. 4, p. 345-350, 2010. Disponível em: <http://brn.sagepub.com/content/11/4/345>. Acesso em: 07 abr. 2011. |
dc.identifier.uri.fl_str_mv |
http://repositorio.furg.br/handle/1/430 |
dc.identifier.issn.none.fl_str_mv |
1552-4175 |
identifier_str_mv |
BAISCH, Ana Luiza Muccillo et al. Evaluation of the Analgesic Effect of Aqueous Extract of Brugmansia suaveolens Flower in Mice: Possible Mechanism Involved. Biological Research for Nursing. v. 11, n. 4, p. 345-350, 2010. Disponível em: <http://brn.sagepub.com/content/11/4/345>. Acesso em: 07 abr. 2011. 1552-4175 |
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http://repositorio.furg.br/handle/1/430 |
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eng |
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eng |
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