Qualidade higiênico-sanitária de formulações ministradas a neonatos

• Autor(a) principal: Nienov, Ana Talita
• Data de Publicação: 2009
• Outros Autores: Macedo, Maíra Bidart de, Felix, Carolina Rodrigues, Soares, Daniela Fernandes Ramos, Moreira, Ângela Nunes, Silva, Pedro Eduardo Almeida da
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da FURG (RI FURG)
• Texto Completo: http://repositorio.furg.br/handle/1/3151
• topic: Infant formula; Contamination; Antimicrobial resistance; Fórmula infantil; Contaminación; Resistencia antimicrobiana; Contaminação; Resistência antimicrobiana

Premature babies hospitalized at the Neonatal Intensive Care Units, particularly those with low weight and that are not allowed to be normally fed, often need nutritional support of Infant Formulas (IF). These formulas, when contaminated, can be the source of nosocomial infections, the most probable way of contamination being the inadequate handling and storage of these formulas. Evaluate the Hygienic-Sanitary quality and the susceptibility profi le of the bacteria isolated from Ifs prepared in the lactary in the city of Rio Grande-RS to antimicrobial agents. IF samples were collected during 6 months by hospital employees working in the morning (Group A)and afternoon (group B) shifts, totalizing 72 analyzed samples. The Most Probable Number method (MPN/ml) was utilized to measure the amount of thermo-resistant and total coliforms. For mesophilic microorganisms the number of Colony-forming Units (CFU/ml) was counted. Also, samples were cultured in Blood and MacConkey Agar media and the isolates were identifi ed by 16S rRNA sequencing and submitted to antibiogram by the Kirby-Bauer method. The samples from groups A and B presented, respectively, 88.9% and 55.5% of contamination by total coliforms and 61.1% e 38.8% by thermo-resistant coliforms. Concerning the count of mesophilic microorganisms, groups A and B presented, respectively, 69.4% and 52.7% of the samples with results above the allowed limit. The identifi ed microorganisms were Escherichia coli, Acinetobacter spp., Pseudomonas spp. and Enterobacter spp. The antimicrobial susceptibility test showed that all isolates presented resistance to at least three tested antimicrobials and that all of the isolates were resistant to tetracycline and one of them was also resistant to a carbapenem (imipinem).