Design Strategies for Integrated b-Galactosidase Purification Processes

Detalhes bibliográficos
Autor(a) principal: Lemes, Ailton Cesar
Data de Publicação: 2014
Outros Autores: Machado, Juliana Ribeiro, Brites, Marcela Lazzare, Di Luccio, Marco, Kalil, Susana Juliano
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FURG (RI FURG)
Texto Completo: http://repositorio.furg.br/handle/1/4633
Resumo: The operational conditions for an aqueous two-phase system (ATPS) for b-galactosidase purification were optimized and applied to the design of a purification strategy as an alternative to the primary purification steps. The ATPS proved to be suitable for the recovery and primary enzyme purification. The purification process design developed by ATPS, diafiltration, ion exchange, and diafiltration/ultrafiltration was successful, yielding a more than tenfold purification. The purification strategy design resulted in a powerful integrated purification and recovery process, an evidence of the potential for a scale-up of the b-galactosidase purification process.
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spelling Design Strategies for Integrated b-Galactosidase Purification ProcessesB-GalactosidasePurificationSeparation techniquesTwo-phase systemThe operational conditions for an aqueous two-phase system (ATPS) for b-galactosidase purification were optimized and applied to the design of a purification strategy as an alternative to the primary purification steps. The ATPS proved to be suitable for the recovery and primary enzyme purification. The purification process design developed by ATPS, diafiltration, ion exchange, and diafiltration/ultrafiltration was successful, yielding a more than tenfold purification. The purification strategy design resulted in a powerful integrated purification and recovery process, an evidence of the potential for a scale-up of the b-galactosidase purification process.2014-10-12T14:04:32Z2014-10-12T14:04:32Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfLEMES, Ailton Cesar et al. Design Strategies for Integrated b-Galactosidase Purification Processes. Chem. Eng. Technol., v. 37, n. 10, p. 1–9, 2014. Disponível em: <http://onlinelibrary.wiley.com/doi/10.1002/ceat.201300433/abstract>. Acesso em: 09 set. 2014.1521-4125http://repositorio.furg.br/handle/1/463310.1002/ceat.201300433engLemes, Ailton CesarMachado, Juliana RibeiroBrites, Marcela LazzareDi Luccio, MarcoKalil, Susana Julianoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURG2014-10-12T14:04:32Zoai:repositorio.furg.br:1/4633Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2014-10-12T14:04:32Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false
dc.title.none.fl_str_mv Design Strategies for Integrated b-Galactosidase Purification Processes
title Design Strategies for Integrated b-Galactosidase Purification Processes
spellingShingle Design Strategies for Integrated b-Galactosidase Purification Processes
Lemes, Ailton Cesar
B-Galactosidase
Purification
Separation techniques
Two-phase system
title_short Design Strategies for Integrated b-Galactosidase Purification Processes
title_full Design Strategies for Integrated b-Galactosidase Purification Processes
title_fullStr Design Strategies for Integrated b-Galactosidase Purification Processes
title_full_unstemmed Design Strategies for Integrated b-Galactosidase Purification Processes
title_sort Design Strategies for Integrated b-Galactosidase Purification Processes
author Lemes, Ailton Cesar
author_facet Lemes, Ailton Cesar
Machado, Juliana Ribeiro
Brites, Marcela Lazzare
Di Luccio, Marco
Kalil, Susana Juliano
author_role author
author2 Machado, Juliana Ribeiro
Brites, Marcela Lazzare
Di Luccio, Marco
Kalil, Susana Juliano
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Lemes, Ailton Cesar
Machado, Juliana Ribeiro
Brites, Marcela Lazzare
Di Luccio, Marco
Kalil, Susana Juliano
dc.subject.por.fl_str_mv B-Galactosidase
Purification
Separation techniques
Two-phase system
topic B-Galactosidase
Purification
Separation techniques
Two-phase system
description The operational conditions for an aqueous two-phase system (ATPS) for b-galactosidase purification were optimized and applied to the design of a purification strategy as an alternative to the primary purification steps. The ATPS proved to be suitable for the recovery and primary enzyme purification. The purification process design developed by ATPS, diafiltration, ion exchange, and diafiltration/ultrafiltration was successful, yielding a more than tenfold purification. The purification strategy design resulted in a powerful integrated purification and recovery process, an evidence of the potential for a scale-up of the b-galactosidase purification process.
publishDate 2014
dc.date.none.fl_str_mv 2014-10-12T14:04:32Z
2014-10-12T14:04:32Z
2014
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv LEMES, Ailton Cesar et al. Design Strategies for Integrated b-Galactosidase Purification Processes. Chem. Eng. Technol., v. 37, n. 10, p. 1–9, 2014. Disponível em: <http://onlinelibrary.wiley.com/doi/10.1002/ceat.201300433/abstract>. Acesso em: 09 set. 2014.
1521-4125
http://repositorio.furg.br/handle/1/4633
10.1002/ceat.201300433
identifier_str_mv LEMES, Ailton Cesar et al. Design Strategies for Integrated b-Galactosidase Purification Processes. Chem. Eng. Technol., v. 37, n. 10, p. 1–9, 2014. Disponível em: <http://onlinelibrary.wiley.com/doi/10.1002/ceat.201300433/abstract>. Acesso em: 09 set. 2014.
1521-4125
10.1002/ceat.201300433
url http://repositorio.furg.br/handle/1/4633
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da FURG (RI FURG)
instname:Universidade Federal do Rio Grande (FURG)
instacron:FURG
instname_str Universidade Federal do Rio Grande (FURG)
instacron_str FURG
institution FURG
reponame_str Repositório Institucional da FURG (RI FURG)
collection Repositório Institucional da FURG (RI FURG)
repository.name.fl_str_mv Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)
repository.mail.fl_str_mv
_version_ 1807384365234126848

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