Design Strategies for Integrated b-Galactosidase Purification Processes
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FURG (RI FURG) |
Texto Completo: | http://repositorio.furg.br/handle/1/4633 |
Resumo: | The operational conditions for an aqueous two-phase system (ATPS) for b-galactosidase purification were optimized and applied to the design of a purification strategy as an alternative to the primary purification steps. The ATPS proved to be suitable for the recovery and primary enzyme purification. The purification process design developed by ATPS, diafiltration, ion exchange, and diafiltration/ultrafiltration was successful, yielding a more than tenfold purification. The purification strategy design resulted in a powerful integrated purification and recovery process, an evidence of the potential for a scale-up of the b-galactosidase purification process. |
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Design Strategies for Integrated b-Galactosidase Purification ProcessesB-GalactosidasePurificationSeparation techniquesTwo-phase systemThe operational conditions for an aqueous two-phase system (ATPS) for b-galactosidase purification were optimized and applied to the design of a purification strategy as an alternative to the primary purification steps. The ATPS proved to be suitable for the recovery and primary enzyme purification. The purification process design developed by ATPS, diafiltration, ion exchange, and diafiltration/ultrafiltration was successful, yielding a more than tenfold purification. The purification strategy design resulted in a powerful integrated purification and recovery process, an evidence of the potential for a scale-up of the b-galactosidase purification process.2014-10-12T14:04:32Z2014-10-12T14:04:32Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfLEMES, Ailton Cesar et al. Design Strategies for Integrated b-Galactosidase Purification Processes. Chem. Eng. Technol., v. 37, n. 10, p. 1–9, 2014. Disponível em: <http://onlinelibrary.wiley.com/doi/10.1002/ceat.201300433/abstract>. Acesso em: 09 set. 2014.1521-4125http://repositorio.furg.br/handle/1/463310.1002/ceat.201300433engLemes, Ailton CesarMachado, Juliana RibeiroBrites, Marcela LazzareDi Luccio, MarcoKalil, Susana Julianoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURG2014-10-12T14:04:32Zoai:repositorio.furg.br:1/4633Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2014-10-12T14:04:32Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false |
dc.title.none.fl_str_mv |
Design Strategies for Integrated b-Galactosidase Purification Processes |
title |
Design Strategies for Integrated b-Galactosidase Purification Processes |
spellingShingle |
Design Strategies for Integrated b-Galactosidase Purification Processes Lemes, Ailton Cesar B-Galactosidase Purification Separation techniques Two-phase system |
title_short |
Design Strategies for Integrated b-Galactosidase Purification Processes |
title_full |
Design Strategies for Integrated b-Galactosidase Purification Processes |
title_fullStr |
Design Strategies for Integrated b-Galactosidase Purification Processes |
title_full_unstemmed |
Design Strategies for Integrated b-Galactosidase Purification Processes |
title_sort |
Design Strategies for Integrated b-Galactosidase Purification Processes |
author |
Lemes, Ailton Cesar |
author_facet |
Lemes, Ailton Cesar Machado, Juliana Ribeiro Brites, Marcela Lazzare Di Luccio, Marco Kalil, Susana Juliano |
author_role |
author |
author2 |
Machado, Juliana Ribeiro Brites, Marcela Lazzare Di Luccio, Marco Kalil, Susana Juliano |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Lemes, Ailton Cesar Machado, Juliana Ribeiro Brites, Marcela Lazzare Di Luccio, Marco Kalil, Susana Juliano |
dc.subject.por.fl_str_mv |
B-Galactosidase Purification Separation techniques Two-phase system |
topic |
B-Galactosidase Purification Separation techniques Two-phase system |
description |
The operational conditions for an aqueous two-phase system (ATPS) for b-galactosidase purification were optimized and applied to the design of a purification strategy as an alternative to the primary purification steps. The ATPS proved to be suitable for the recovery and primary enzyme purification. The purification process design developed by ATPS, diafiltration, ion exchange, and diafiltration/ultrafiltration was successful, yielding a more than tenfold purification. The purification strategy design resulted in a powerful integrated purification and recovery process, an evidence of the potential for a scale-up of the b-galactosidase purification process. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-10-12T14:04:32Z 2014-10-12T14:04:32Z 2014 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
LEMES, Ailton Cesar et al. Design Strategies for Integrated b-Galactosidase Purification Processes. Chem. Eng. Technol., v. 37, n. 10, p. 1–9, 2014. Disponível em: <http://onlinelibrary.wiley.com/doi/10.1002/ceat.201300433/abstract>. Acesso em: 09 set. 2014. 1521-4125 http://repositorio.furg.br/handle/1/4633 10.1002/ceat.201300433 |
identifier_str_mv |
LEMES, Ailton Cesar et al. Design Strategies for Integrated b-Galactosidase Purification Processes. Chem. Eng. Technol., v. 37, n. 10, p. 1–9, 2014. Disponível em: <http://onlinelibrary.wiley.com/doi/10.1002/ceat.201300433/abstract>. Acesso em: 09 set. 2014. 1521-4125 10.1002/ceat.201300433 |
url |
http://repositorio.furg.br/handle/1/4633 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FURG (RI FURG) instname:Universidade Federal do Rio Grande (FURG) instacron:FURG |
instname_str |
Universidade Federal do Rio Grande (FURG) |
instacron_str |
FURG |
institution |
FURG |
reponame_str |
Repositório Institucional da FURG (RI FURG) |
collection |
Repositório Institucional da FURG (RI FURG) |
repository.name.fl_str_mv |
Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG) |
repository.mail.fl_str_mv |
|
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1813187236377657344 |