Prevalence of Lettuce mosaic virus - common strain on three lettuce producing areas from São Paulo State
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Summa phytopathologica (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052008000200009 |
Resumo: | LMV is one of the most important pathogens of lettuce worldwide. Based on their ability to overcome the resistance genes mo1¹ and mo1² in lettuce, isolates can be divided in two types: LMV-Most, which can infect and are seed-borne in cultivars containing the mo1 gene and LMV-Common, which do not cause symptoms on these cultivars and are seed transmitted only in susceptible cultivars. To evaluate the occurrence of these two types of LMV isolates, a survey was carried out during 2002-2005 in three lettuce production areas from São Paulo State. Total RNA was used for the diagnosis of LMV isolates by RT-PCR using universal primers for the variable N-terminus of the capsid protein, in the 3' end of the genome. Positives samples were analyzed by a second RT-PCR using specifics primers for LMV-Most isolates designed to amplify a fragment from the central region (CI-VPg) of the genome. A total of 1362 samples showing mosaic symptoms were collected and 504 (37.29 %) were positives for LMV. On susceptible lettuce cultivars, LMV-Common was prevalent (77.3%). LMV-Most was found frequently associated with tolerant (mo1¹) lettuce cultivars. Susceptible cultivars correspond today for most of the area of lettuce production. So, despite the ability of LMV-Most isolates to overcome the resistance provided by the recessive mo1¹ gene, they are not prevalent in the conditions of São Paulo State. |
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Prevalence of Lettuce mosaic virus - common strain on three lettuce producing areas from São Paulo StatePotyvirusLMVRT-PCRdetectionresistance breakingLMV is one of the most important pathogens of lettuce worldwide. Based on their ability to overcome the resistance genes mo1¹ and mo1² in lettuce, isolates can be divided in two types: LMV-Most, which can infect and are seed-borne in cultivars containing the mo1 gene and LMV-Common, which do not cause symptoms on these cultivars and are seed transmitted only in susceptible cultivars. To evaluate the occurrence of these two types of LMV isolates, a survey was carried out during 2002-2005 in three lettuce production areas from São Paulo State. Total RNA was used for the diagnosis of LMV isolates by RT-PCR using universal primers for the variable N-terminus of the capsid protein, in the 3' end of the genome. Positives samples were analyzed by a second RT-PCR using specifics primers for LMV-Most isolates designed to amplify a fragment from the central region (CI-VPg) of the genome. A total of 1362 samples showing mosaic symptoms were collected and 504 (37.29 %) were positives for LMV. On susceptible lettuce cultivars, LMV-Common was prevalent (77.3%). LMV-Most was found frequently associated with tolerant (mo1¹) lettuce cultivars. Susceptible cultivars correspond today for most of the area of lettuce production. So, despite the ability of LMV-Most isolates to overcome the resistance provided by the recessive mo1¹ gene, they are not prevalent in the conditions of São Paulo State.Grupo Paulista de Fitopatologia2008-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052008000200009Summa Phytopathologica v.34 n.2 2008reponame:Summa phytopathologica (Online)instname:Grupo Paulista de Fitopatologiainstacron:GPF10.1590/S0100-54052008000200009info:eu-repo/semantics/openAccessFirmino,Ana CarolinaKrause-Sakate,RenatePavan,Marcelo AgenorSilva,Norberto daHanai,Sérgio MinoruAnbo,Roberto HirotoNietzsche,ThomasLe Gall,Oliviereng2008-07-31T00:00:00Zoai:scielo:S0100-54052008000200009Revistahttp://www.scielo.br/sphttps://old.scielo.br/oai/scielo-oai.phpsumma@fca.unesp.br1980-54540100-5405opendoar:2008-07-31T00:00Summa phytopathologica (Online) - Grupo Paulista de Fitopatologiafalse |
dc.title.none.fl_str_mv |
Prevalence of Lettuce mosaic virus - common strain on three lettuce producing areas from São Paulo State |
title |
Prevalence of Lettuce mosaic virus - common strain on three lettuce producing areas from São Paulo State |
spellingShingle |
Prevalence of Lettuce mosaic virus - common strain on three lettuce producing areas from São Paulo State Firmino,Ana Carolina Potyvirus LMV RT-PCR detection resistance breaking |
title_short |
Prevalence of Lettuce mosaic virus - common strain on three lettuce producing areas from São Paulo State |
title_full |
Prevalence of Lettuce mosaic virus - common strain on three lettuce producing areas from São Paulo State |
title_fullStr |
Prevalence of Lettuce mosaic virus - common strain on three lettuce producing areas from São Paulo State |
title_full_unstemmed |
Prevalence of Lettuce mosaic virus - common strain on three lettuce producing areas from São Paulo State |
title_sort |
Prevalence of Lettuce mosaic virus - common strain on three lettuce producing areas from São Paulo State |
author |
Firmino,Ana Carolina |
author_facet |
Firmino,Ana Carolina Krause-Sakate,Renate Pavan,Marcelo Agenor Silva,Norberto da Hanai,Sérgio Minoru Anbo,Roberto Hiroto Nietzsche,Thomas Le Gall,Olivier |
author_role |
author |
author2 |
Krause-Sakate,Renate Pavan,Marcelo Agenor Silva,Norberto da Hanai,Sérgio Minoru Anbo,Roberto Hiroto Nietzsche,Thomas Le Gall,Olivier |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Firmino,Ana Carolina Krause-Sakate,Renate Pavan,Marcelo Agenor Silva,Norberto da Hanai,Sérgio Minoru Anbo,Roberto Hiroto Nietzsche,Thomas Le Gall,Olivier |
dc.subject.por.fl_str_mv |
Potyvirus LMV RT-PCR detection resistance breaking |
topic |
Potyvirus LMV RT-PCR detection resistance breaking |
description |
LMV is one of the most important pathogens of lettuce worldwide. Based on their ability to overcome the resistance genes mo1¹ and mo1² in lettuce, isolates can be divided in two types: LMV-Most, which can infect and are seed-borne in cultivars containing the mo1 gene and LMV-Common, which do not cause symptoms on these cultivars and are seed transmitted only in susceptible cultivars. To evaluate the occurrence of these two types of LMV isolates, a survey was carried out during 2002-2005 in three lettuce production areas from São Paulo State. Total RNA was used for the diagnosis of LMV isolates by RT-PCR using universal primers for the variable N-terminus of the capsid protein, in the 3' end of the genome. Positives samples were analyzed by a second RT-PCR using specifics primers for LMV-Most isolates designed to amplify a fragment from the central region (CI-VPg) of the genome. A total of 1362 samples showing mosaic symptoms were collected and 504 (37.29 %) were positives for LMV. On susceptible lettuce cultivars, LMV-Common was prevalent (77.3%). LMV-Most was found frequently associated with tolerant (mo1¹) lettuce cultivars. Susceptible cultivars correspond today for most of the area of lettuce production. So, despite the ability of LMV-Most isolates to overcome the resistance provided by the recessive mo1¹ gene, they are not prevalent in the conditions of São Paulo State. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052008000200009 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052008000200009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-54052008000200009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Grupo Paulista de Fitopatologia |
publisher.none.fl_str_mv |
Grupo Paulista de Fitopatologia |
dc.source.none.fl_str_mv |
Summa Phytopathologica v.34 n.2 2008 reponame:Summa phytopathologica (Online) instname:Grupo Paulista de Fitopatologia instacron:GPF |
instname_str |
Grupo Paulista de Fitopatologia |
instacron_str |
GPF |
institution |
GPF |
reponame_str |
Summa phytopathologica (Online) |
collection |
Summa phytopathologica (Online) |
repository.name.fl_str_mv |
Summa phytopathologica (Online) - Grupo Paulista de Fitopatologia |
repository.mail.fl_str_mv |
summa@fca.unesp.br |
_version_ |
1754193415889747968 |