Induction of defense enzymes and control of anthracnose in cucumber by Corymbia citriodora aqueous extract

• Autor(a) principal: Franzener,Gilmar
• Data de Publicação: 2018
• Outros Autores: Schwan-Estrada,Kátia Regina Freitas, Moura,Gabriela Silva, Kuhn,Odair José, Stangarlin,José Renato
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Summa phytopathologica (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052018000100010
• topic: Resistance induction; alternative control; medicinal plant

ABSTRACT Secondary compounds of medicinal plants can activate defense mechanisms in plants against pathogens. The aqueous extract (AE) of Corymbia citriodora has shown that activity, but there is scarce information about the involved mechanisms of action. Therefore, this study aimed to evaluate the effect of AE on the induction of defense enzymes and protection of cucumber from Colletotrichum lagenarium. Thus, the AE, autoclaved or not, was evaluated for its capability of protecting and inducing peroxidases, polyphenoloxidases, chitinases and β-1.3-glucanases, and phenylalanine ammonia-lyase. Distilled water and acibenzolar-S-methyl (50 mg a.i. L-1) were used as controls. The effect of AE at the concentrations of 1, 5, 10, 15 and 20% was also evaluated, as well as its local and systemic effect, and the effect of one or two applications. Pathogen inoculation or sample collection for determination of enzyme activity was performed at 72 hours after treatments. The AE reduced the severity of C. lagenarium and induced peroxidases and β-1.3-glucanases by 37.6, 67.2 and 122.7%, respectively. There was a reduction in the disease severity and an increase in peroxidases from the concentration of 5% AE. The latter showed only local effect and greater reduction in severity when two applications were performed. These results suggest the effect of AE in inducing resistance in cucumber and inducing peroxidases and β-1.3-glucanases.