Differentiation of Colletotrichum gloeosporioides isolates by using total proteins and esterase electrophoretic patterns and extracellular enzymes production

Detalhes bibliográficos
Autor(a) principal: Assis,Tereza Cristina de
Data de Publicação: 2010
Outros Autores: Menezes,Maria, Andrade,Domingos Eduardo Guimarães Tavares de, Coelho,Rildo Sartori Barbosa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Summa phytopathologica (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052010000200006
Resumo: Isolates of Colletotrichum gloeosporioides (ISO-1, ISO-2, ISO-3, ISO-4, ISO-5 and ISO-6), the causal agent of anthracnose disease on mango fruits, were characterized by electrophoretic patterns of total proteins and esterase in polyacrylamida gel, and also, by production of extracellular enzymes on specific solid substrate. The electrophoretic analysis showed variation in number, intensity of coloration and position of the bands in the gel at each studied system tested. In contrast to the monomorphic behavior to total proteins, high esterase polymorfism was observed indicating difference among isolates. All isolates showed the activity of extracellular enzymes such as amylase, lipase, and protease with some variation among them. The proteolitic activity seemed to be more accentuated than the two other enzymes studied.
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spelling Differentiation of Colletotrichum gloeosporioides isolates by using total proteins and esterase electrophoretic patterns and extracellular enzymes productionMangifera indicaproteinsisoenzymeshydrolytic activityanthracnoseIsolates of Colletotrichum gloeosporioides (ISO-1, ISO-2, ISO-3, ISO-4, ISO-5 and ISO-6), the causal agent of anthracnose disease on mango fruits, were characterized by electrophoretic patterns of total proteins and esterase in polyacrylamida gel, and also, by production of extracellular enzymes on specific solid substrate. The electrophoretic analysis showed variation in number, intensity of coloration and position of the bands in the gel at each studied system tested. In contrast to the monomorphic behavior to total proteins, high esterase polymorfism was observed indicating difference among isolates. All isolates showed the activity of extracellular enzymes such as amylase, lipase, and protease with some variation among them. The proteolitic activity seemed to be more accentuated than the two other enzymes studied.Grupo Paulista de Fitopatologia2010-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052010000200006Summa Phytopathologica v.36 n.2 2010reponame:Summa phytopathologica (Online)instname:Grupo Paulista de Fitopatologiainstacron:GPF10.1590/S0100-54052010000200006info:eu-repo/semantics/openAccessAssis,Tereza Cristina deMenezes,MariaAndrade,Domingos Eduardo Guimarães Tavares deCoelho,Rildo Sartori Barbosaeng2010-07-20T00:00:00Zoai:scielo:S0100-54052010000200006Revistahttp://www.scielo.br/sphttps://old.scielo.br/oai/scielo-oai.phpsumma@fca.unesp.br1980-54540100-5405opendoar:2010-07-20T00:00Summa phytopathologica (Online) - Grupo Paulista de Fitopatologiafalse
dc.title.none.fl_str_mv Differentiation of Colletotrichum gloeosporioides isolates by using total proteins and esterase electrophoretic patterns and extracellular enzymes production
title Differentiation of Colletotrichum gloeosporioides isolates by using total proteins and esterase electrophoretic patterns and extracellular enzymes production
spellingShingle Differentiation of Colletotrichum gloeosporioides isolates by using total proteins and esterase electrophoretic patterns and extracellular enzymes production
Assis,Tereza Cristina de
Mangifera indica
proteins
isoenzymes
hydrolytic activity
anthracnose
title_short Differentiation of Colletotrichum gloeosporioides isolates by using total proteins and esterase electrophoretic patterns and extracellular enzymes production
title_full Differentiation of Colletotrichum gloeosporioides isolates by using total proteins and esterase electrophoretic patterns and extracellular enzymes production
title_fullStr Differentiation of Colletotrichum gloeosporioides isolates by using total proteins and esterase electrophoretic patterns and extracellular enzymes production
title_full_unstemmed Differentiation of Colletotrichum gloeosporioides isolates by using total proteins and esterase electrophoretic patterns and extracellular enzymes production
title_sort Differentiation of Colletotrichum gloeosporioides isolates by using total proteins and esterase electrophoretic patterns and extracellular enzymes production
author Assis,Tereza Cristina de
author_facet Assis,Tereza Cristina de
Menezes,Maria
Andrade,Domingos Eduardo Guimarães Tavares de
Coelho,Rildo Sartori Barbosa
author_role author
author2 Menezes,Maria
Andrade,Domingos Eduardo Guimarães Tavares de
Coelho,Rildo Sartori Barbosa
author2_role author
author
author
dc.contributor.author.fl_str_mv Assis,Tereza Cristina de
Menezes,Maria
Andrade,Domingos Eduardo Guimarães Tavares de
Coelho,Rildo Sartori Barbosa
dc.subject.por.fl_str_mv Mangifera indica
proteins
isoenzymes
hydrolytic activity
anthracnose
topic Mangifera indica
proteins
isoenzymes
hydrolytic activity
anthracnose
description Isolates of Colletotrichum gloeosporioides (ISO-1, ISO-2, ISO-3, ISO-4, ISO-5 and ISO-6), the causal agent of anthracnose disease on mango fruits, were characterized by electrophoretic patterns of total proteins and esterase in polyacrylamida gel, and also, by production of extracellular enzymes on specific solid substrate. The electrophoretic analysis showed variation in number, intensity of coloration and position of the bands in the gel at each studied system tested. In contrast to the monomorphic behavior to total proteins, high esterase polymorfism was observed indicating difference among isolates. All isolates showed the activity of extracellular enzymes such as amylase, lipase, and protease with some variation among them. The proteolitic activity seemed to be more accentuated than the two other enzymes studied.
publishDate 2010
dc.date.none.fl_str_mv 2010-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052010000200006
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052010000200006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-54052010000200006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Grupo Paulista de Fitopatologia
publisher.none.fl_str_mv Grupo Paulista de Fitopatologia
dc.source.none.fl_str_mv Summa Phytopathologica v.36 n.2 2010
reponame:Summa phytopathologica (Online)
instname:Grupo Paulista de Fitopatologia
instacron:GPF
instname_str Grupo Paulista de Fitopatologia
instacron_str GPF
institution GPF
reponame_str Summa phytopathologica (Online)
collection Summa phytopathologica (Online)
repository.name.fl_str_mv Summa phytopathologica (Online) - Grupo Paulista de Fitopatologia
repository.mail.fl_str_mv summa@fca.unesp.br
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