Inoculum production and evaluation of temperature and leaf wetness for Passalora sojina inoculation in soybean
Main Author: | |
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Publication Date: | 2018 |
Other Authors: | , , , |
Format: | Article |
Language: | eng |
Source: | Summa phytopathologica (Online) |
Download full: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052018000200122 |
Summary: | ABSTRACT The objective of this study was to develop a methodology for inoculum production and inoculation of Passalora sojina in soybean to assess the reaction to frogeye leaf spot. Thus, sporulation of fungal races was quantified in five substrates under two light regimes. The temperature and the leaf wetness period that resulted in greater infection efficiency were also determined. Petri dishes containing the culture media Oat Flour Agar (OFA), Tomato Juice (FTJ), V8 agar juice (V8), Infant Food (IF) and Potato Sucrose Agar (PSA) plus isolates of P. sojina races 23, 24 and 25 were subjected to 12-h photoperiod and 24-h continuous dark. Inoculated plants were incubated at temperatures of 15, 20, 25, 30 and 35 ºC and leaf wetness periods of 12, 24, 36, 48 and 72 hours. Experimental design was completely randomized with five replicates. There was interaction among isolate, culture medium and photoperiod. The highest sporulation (conidia/cm2) was obtained in the culture media FTJ (race 23) and V8 (races 24 and 25) under 12-h photoperiod. The optimum temperature for the disease development was 27oC under 72 hours of continuous leaf wetness. |
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Inoculum production and evaluation of temperature and leaf wetness for Passalora sojina inoculation in soybeansporulationfrogeye leaf spotleaf wetnessinfection critical periodABSTRACT The objective of this study was to develop a methodology for inoculum production and inoculation of Passalora sojina in soybean to assess the reaction to frogeye leaf spot. Thus, sporulation of fungal races was quantified in five substrates under two light regimes. The temperature and the leaf wetness period that resulted in greater infection efficiency were also determined. Petri dishes containing the culture media Oat Flour Agar (OFA), Tomato Juice (FTJ), V8 agar juice (V8), Infant Food (IF) and Potato Sucrose Agar (PSA) plus isolates of P. sojina races 23, 24 and 25 were subjected to 12-h photoperiod and 24-h continuous dark. Inoculated plants were incubated at temperatures of 15, 20, 25, 30 and 35 ºC and leaf wetness periods of 12, 24, 36, 48 and 72 hours. Experimental design was completely randomized with five replicates. There was interaction among isolate, culture medium and photoperiod. The highest sporulation (conidia/cm2) was obtained in the culture media FTJ (race 23) and V8 (races 24 and 25) under 12-h photoperiod. The optimum temperature for the disease development was 27oC under 72 hours of continuous leaf wetness.Grupo Paulista de Fitopatologia2018-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052018000200122Summa Phytopathologica v.44 n.2 2018reponame:Summa phytopathologica (Online)instname:Grupo Paulista de Fitopatologiainstacron:GPF10.1590/0100-5405/168573info:eu-repo/semantics/openAccessCamera,Juliane NicolodiDeuner,Carolina CardosoGhissi,Valéria CecíliaReis,Erlei MeloKoefender,Janaeng2018-06-07T00:00:00Zoai:scielo:S0100-54052018000200122Revistahttp://www.scielo.br/sphttps://old.scielo.br/oai/scielo-oai.phpsumma@fca.unesp.br1980-54540100-5405opendoar:2018-06-07T00:00Summa phytopathologica (Online) - Grupo Paulista de Fitopatologiafalse |
dc.title.none.fl_str_mv |
Inoculum production and evaluation of temperature and leaf wetness for Passalora sojina inoculation in soybean |
title |
Inoculum production and evaluation of temperature and leaf wetness for Passalora sojina inoculation in soybean |
spellingShingle |
Inoculum production and evaluation of temperature and leaf wetness for Passalora sojina inoculation in soybean Camera,Juliane Nicolodi sporulation frogeye leaf spot leaf wetness infection critical period |
title_short |
Inoculum production and evaluation of temperature and leaf wetness for Passalora sojina inoculation in soybean |
title_full |
Inoculum production and evaluation of temperature and leaf wetness for Passalora sojina inoculation in soybean |
title_fullStr |
Inoculum production and evaluation of temperature and leaf wetness for Passalora sojina inoculation in soybean |
title_full_unstemmed |
Inoculum production and evaluation of temperature and leaf wetness for Passalora sojina inoculation in soybean |
title_sort |
Inoculum production and evaluation of temperature and leaf wetness for Passalora sojina inoculation in soybean |
author |
Camera,Juliane Nicolodi |
author_facet |
Camera,Juliane Nicolodi Deuner,Carolina Cardoso Ghissi,Valéria Cecília Reis,Erlei Melo Koefender,Jana |
author_role |
author |
author2 |
Deuner,Carolina Cardoso Ghissi,Valéria Cecília Reis,Erlei Melo Koefender,Jana |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Camera,Juliane Nicolodi Deuner,Carolina Cardoso Ghissi,Valéria Cecília Reis,Erlei Melo Koefender,Jana |
dc.subject.por.fl_str_mv |
sporulation frogeye leaf spot leaf wetness infection critical period |
topic |
sporulation frogeye leaf spot leaf wetness infection critical period |
description |
ABSTRACT The objective of this study was to develop a methodology for inoculum production and inoculation of Passalora sojina in soybean to assess the reaction to frogeye leaf spot. Thus, sporulation of fungal races was quantified in five substrates under two light regimes. The temperature and the leaf wetness period that resulted in greater infection efficiency were also determined. Petri dishes containing the culture media Oat Flour Agar (OFA), Tomato Juice (FTJ), V8 agar juice (V8), Infant Food (IF) and Potato Sucrose Agar (PSA) plus isolates of P. sojina races 23, 24 and 25 were subjected to 12-h photoperiod and 24-h continuous dark. Inoculated plants were incubated at temperatures of 15, 20, 25, 30 and 35 ºC and leaf wetness periods of 12, 24, 36, 48 and 72 hours. Experimental design was completely randomized with five replicates. There was interaction among isolate, culture medium and photoperiod. The highest sporulation (conidia/cm2) was obtained in the culture media FTJ (race 23) and V8 (races 24 and 25) under 12-h photoperiod. The optimum temperature for the disease development was 27oC under 72 hours of continuous leaf wetness. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052018000200122 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052018000200122 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0100-5405/168573 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Grupo Paulista de Fitopatologia |
publisher.none.fl_str_mv |
Grupo Paulista de Fitopatologia |
dc.source.none.fl_str_mv |
Summa Phytopathologica v.44 n.2 2018 reponame:Summa phytopathologica (Online) instname:Grupo Paulista de Fitopatologia instacron:GPF |
instname_str |
Grupo Paulista de Fitopatologia |
instacron_str |
GPF |
institution |
GPF |
reponame_str |
Summa phytopathologica (Online) |
collection |
Summa phytopathologica (Online) |
repository.name.fl_str_mv |
Summa phytopathologica (Online) - Grupo Paulista de Fitopatologia |
repository.mail.fl_str_mv |
summa@fca.unesp.br |
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1754193419126702080 |