Role of N-acetylcysteine on fibrosis and oxidative stress in cirrhotic rats

Detalhes bibliográficos
Autor(a) principal: Pereira-Filho,Gustavo
Data de Publicação: 2008
Outros Autores: Ferreira,Clarissa, Schwengber,Alex, Marroni,Cláudio, Zettler,Cláudio, Marroni,Norma
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Arquivos de gastroenterologia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-28032008000200013
Resumo: BACKGROUND: Hepatic cirrhosis is the final stage of liver dysfunction, characterized by diffuse fibrosis which is the main response to the liver injury. The inhalatory carbon tetrachloride is an effective experimental model that triggers cirrhosis and allows to obtain histological and physiological modifications similar to the one seen in humans. AIM: To investigate the effects of N-acetylcysteine (NAC) on the fibrosis and oxidative stress in the liver of cirrhotic rats, analyzing liver function tests, lipoperoxidation, activity of glutathione peroxidase enzyme, collagen quantification, histopathology, as well as the nitric oxide role. METHODS: The animals were randomly in three experimentals groups: control (CO); cirrhotic (CCl4) and CCl4 + NAC. Evaluate the lipid peroxidation, the glutathione peroxidase enzyme, the collagen and the expression of inducible nitric oxide synthase (iNOS). RESULTS: The cirrhotic group treated with N-acetylcysteine showed trough the histological analysis and collagen quantification lower degrees of fibrosis. This group has also shown less damage to the cellular membranes, less decrease on the glutathione peroxidase levels and less expression of inducible nitric oxide synthase when matched with the cirrhotic group without treatment. CONCLUSION: N-acetylcysteine seams to offer protection against hepatic fibrosis and oxidative stress in cirrhotic rat livers.
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spelling Role of N-acetylcysteine on fibrosis and oxidative stress in cirrhotic ratsLiver cirrhosis, experimentalFibrosisOxidative stressCarbon tetrachlorideAcetylcysteineRatsBACKGROUND: Hepatic cirrhosis is the final stage of liver dysfunction, characterized by diffuse fibrosis which is the main response to the liver injury. The inhalatory carbon tetrachloride is an effective experimental model that triggers cirrhosis and allows to obtain histological and physiological modifications similar to the one seen in humans. AIM: To investigate the effects of N-acetylcysteine (NAC) on the fibrosis and oxidative stress in the liver of cirrhotic rats, analyzing liver function tests, lipoperoxidation, activity of glutathione peroxidase enzyme, collagen quantification, histopathology, as well as the nitric oxide role. METHODS: The animals were randomly in three experimentals groups: control (CO); cirrhotic (CCl4) and CCl4 + NAC. Evaluate the lipid peroxidation, the glutathione peroxidase enzyme, the collagen and the expression of inducible nitric oxide synthase (iNOS). RESULTS: The cirrhotic group treated with N-acetylcysteine showed trough the histological analysis and collagen quantification lower degrees of fibrosis. This group has also shown less damage to the cellular membranes, less decrease on the glutathione peroxidase levels and less expression of inducible nitric oxide synthase when matched with the cirrhotic group without treatment. CONCLUSION: N-acetylcysteine seams to offer protection against hepatic fibrosis and oxidative stress in cirrhotic rat livers.Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia e Outras Especialidades - IBEPEGE. 2008-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-28032008000200013Arquivos de Gastroenterologia v.45 n.2 2008reponame:Arquivos de gastroenterologia (Online)instname:Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologiainstacron:IBEPEGE10.1590/S0004-28032008000200013info:eu-repo/semantics/openAccessPereira-Filho,GustavoFerreira,ClarissaSchwengber,AlexMarroni,CláudioZettler,CláudioMarroni,Normaeng2008-07-07T00:00:00Zoai:scielo:S0004-28032008000200013Revistahttp://www.scielo.br/aghttps://old.scielo.br/oai/scielo-oai.php||secretariaarqgastr@hospitaligesp.com.br1678-42190004-2803opendoar:2008-07-07T00:00Arquivos de gastroenterologia (Online) - Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologiafalse
dc.title.none.fl_str_mv Role of N-acetylcysteine on fibrosis and oxidative stress in cirrhotic rats
title Role of N-acetylcysteine on fibrosis and oxidative stress in cirrhotic rats
spellingShingle Role of N-acetylcysteine on fibrosis and oxidative stress in cirrhotic rats
Pereira-Filho,Gustavo
Liver cirrhosis, experimental
Fibrosis
Oxidative stress
Carbon tetrachloride
Acetylcysteine
Rats
title_short Role of N-acetylcysteine on fibrosis and oxidative stress in cirrhotic rats
title_full Role of N-acetylcysteine on fibrosis and oxidative stress in cirrhotic rats
title_fullStr Role of N-acetylcysteine on fibrosis and oxidative stress in cirrhotic rats
title_full_unstemmed Role of N-acetylcysteine on fibrosis and oxidative stress in cirrhotic rats
title_sort Role of N-acetylcysteine on fibrosis and oxidative stress in cirrhotic rats
author Pereira-Filho,Gustavo
author_facet Pereira-Filho,Gustavo
Ferreira,Clarissa
Schwengber,Alex
Marroni,Cláudio
Zettler,Cláudio
Marroni,Norma
author_role author
author2 Ferreira,Clarissa
Schwengber,Alex
Marroni,Cláudio
Zettler,Cláudio
Marroni,Norma
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Pereira-Filho,Gustavo
Ferreira,Clarissa
Schwengber,Alex
Marroni,Cláudio
Zettler,Cláudio
Marroni,Norma
dc.subject.por.fl_str_mv Liver cirrhosis, experimental
Fibrosis
Oxidative stress
Carbon tetrachloride
Acetylcysteine
Rats
topic Liver cirrhosis, experimental
Fibrosis
Oxidative stress
Carbon tetrachloride
Acetylcysteine
Rats
description BACKGROUND: Hepatic cirrhosis is the final stage of liver dysfunction, characterized by diffuse fibrosis which is the main response to the liver injury. The inhalatory carbon tetrachloride is an effective experimental model that triggers cirrhosis and allows to obtain histological and physiological modifications similar to the one seen in humans. AIM: To investigate the effects of N-acetylcysteine (NAC) on the fibrosis and oxidative stress in the liver of cirrhotic rats, analyzing liver function tests, lipoperoxidation, activity of glutathione peroxidase enzyme, collagen quantification, histopathology, as well as the nitric oxide role. METHODS: The animals were randomly in three experimentals groups: control (CO); cirrhotic (CCl4) and CCl4 + NAC. Evaluate the lipid peroxidation, the glutathione peroxidase enzyme, the collagen and the expression of inducible nitric oxide synthase (iNOS). RESULTS: The cirrhotic group treated with N-acetylcysteine showed trough the histological analysis and collagen quantification lower degrees of fibrosis. This group has also shown less damage to the cellular membranes, less decrease on the glutathione peroxidase levels and less expression of inducible nitric oxide synthase when matched with the cirrhotic group without treatment. CONCLUSION: N-acetylcysteine seams to offer protection against hepatic fibrosis and oxidative stress in cirrhotic rat livers.
publishDate 2008
dc.date.none.fl_str_mv 2008-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-28032008000200013
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-28032008000200013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0004-28032008000200013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia e Outras Especialidades - IBEPEGE.
publisher.none.fl_str_mv Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia e Outras Especialidades - IBEPEGE.
dc.source.none.fl_str_mv Arquivos de Gastroenterologia v.45 n.2 2008
reponame:Arquivos de gastroenterologia (Online)
instname:Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia
instacron:IBEPEGE
instname_str Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia
instacron_str IBEPEGE
institution IBEPEGE
reponame_str Arquivos de gastroenterologia (Online)
collection Arquivos de gastroenterologia (Online)
repository.name.fl_str_mv Arquivos de gastroenterologia (Online) - Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia
repository.mail.fl_str_mv ||secretariaarqgastr@hospitaligesp.com.br
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