Relationship between serum transforming growth factor ß1 and liver collagen content in rats treated with carbon tetrachloride

Detalhes bibliográficos
Autor(a) principal: Oliveira,Fernanda dos Santos
Data de Publicação: 2012
Outros Autores: Silveira,Themis Reverbel da, Matte,Ursula
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Arquivos de gastroenterologia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-28032012000300013
Resumo: CONTEXT: Transforming Growth Factor ß1 (TGFß1) plays a fundamental role in fibrogenesis, although its importance as a biomarker of liver disease is still matter of debate. OBJECTIVE: Quantify serum TGFß1 and its association to liver collagen content in rats exposed to Carbon Tetrachloride (CCl4). METHODS: Rats were submitted to a fibrosis model using CCl4 and sacrificed after 6, 10, 12 and 16 weeks of treatment. Serum levels of TGFß1 were measured by ELISA and collagen content was defined by morphometric analysis. RESULTS: Serum levels of TGFß1 increased between 6 and 10 weeks, whereas collagen density increased between 12 and 16 weeks. A negative correlation was observed between liver collagen deposition and serum levels of TGFß1 (r = -0. 48; P<0. 05). CONCLUSION: Serum levels of TGFß1 were inversely proportional to collagen intensity in cirrhotic livers of rats exposed to CCl4, thus suggesting a limited use as biomarker in advanced liver disease.
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spelling Relationship between serum transforming growth factor ß1 and liver collagen content in rats treated with carbon tetrachlorideLiver cirrhosis, experimentalTransforming growth factor beta 1CollagenCarbon TetrachlorideRatsCONTEXT: Transforming Growth Factor ß1 (TGFß1) plays a fundamental role in fibrogenesis, although its importance as a biomarker of liver disease is still matter of debate. OBJECTIVE: Quantify serum TGFß1 and its association to liver collagen content in rats exposed to Carbon Tetrachloride (CCl4). METHODS: Rats were submitted to a fibrosis model using CCl4 and sacrificed after 6, 10, 12 and 16 weeks of treatment. Serum levels of TGFß1 were measured by ELISA and collagen content was defined by morphometric analysis. RESULTS: Serum levels of TGFß1 increased between 6 and 10 weeks, whereas collagen density increased between 12 and 16 weeks. A negative correlation was observed between liver collagen deposition and serum levels of TGFß1 (r = -0. 48; P<0. 05). CONCLUSION: Serum levels of TGFß1 were inversely proportional to collagen intensity in cirrhotic livers of rats exposed to CCl4, thus suggesting a limited use as biomarker in advanced liver disease.Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia e Outras Especialidades - IBEPEGE. 2012-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-28032012000300013Arquivos de Gastroenterologia v.49 n.3 2012reponame:Arquivos de gastroenterologia (Online)instname:Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologiainstacron:IBEPEGE10.1590/S0004-28032012000300013info:eu-repo/semantics/openAccessOliveira,Fernanda dos SantosSilveira,Themis Reverbel daMatte,Ursulaeng2012-09-21T00:00:00Zoai:scielo:S0004-28032012000300013Revistahttp://www.scielo.br/aghttps://old.scielo.br/oai/scielo-oai.php||secretariaarqgastr@hospitaligesp.com.br1678-42190004-2803opendoar:2012-09-21T00:00Arquivos de gastroenterologia (Online) - Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologiafalse
dc.title.none.fl_str_mv Relationship between serum transforming growth factor ß1 and liver collagen content in rats treated with carbon tetrachloride
title Relationship between serum transforming growth factor ß1 and liver collagen content in rats treated with carbon tetrachloride
spellingShingle Relationship between serum transforming growth factor ß1 and liver collagen content in rats treated with carbon tetrachloride
Oliveira,Fernanda dos Santos
Liver cirrhosis, experimental
Transforming growth factor beta 1
Collagen
Carbon Tetrachloride
Rats
title_short Relationship between serum transforming growth factor ß1 and liver collagen content in rats treated with carbon tetrachloride
title_full Relationship between serum transforming growth factor ß1 and liver collagen content in rats treated with carbon tetrachloride
title_fullStr Relationship between serum transforming growth factor ß1 and liver collagen content in rats treated with carbon tetrachloride
title_full_unstemmed Relationship between serum transforming growth factor ß1 and liver collagen content in rats treated with carbon tetrachloride
title_sort Relationship between serum transforming growth factor ß1 and liver collagen content in rats treated with carbon tetrachloride
author Oliveira,Fernanda dos Santos
author_facet Oliveira,Fernanda dos Santos
Silveira,Themis Reverbel da
Matte,Ursula
author_role author
author2 Silveira,Themis Reverbel da
Matte,Ursula
author2_role author
author
dc.contributor.author.fl_str_mv Oliveira,Fernanda dos Santos
Silveira,Themis Reverbel da
Matte,Ursula
dc.subject.por.fl_str_mv Liver cirrhosis, experimental
Transforming growth factor beta 1
Collagen
Carbon Tetrachloride
Rats
topic Liver cirrhosis, experimental
Transforming growth factor beta 1
Collagen
Carbon Tetrachloride
Rats
description CONTEXT: Transforming Growth Factor ß1 (TGFß1) plays a fundamental role in fibrogenesis, although its importance as a biomarker of liver disease is still matter of debate. OBJECTIVE: Quantify serum TGFß1 and its association to liver collagen content in rats exposed to Carbon Tetrachloride (CCl4). METHODS: Rats were submitted to a fibrosis model using CCl4 and sacrificed after 6, 10, 12 and 16 weeks of treatment. Serum levels of TGFß1 were measured by ELISA and collagen content was defined by morphometric analysis. RESULTS: Serum levels of TGFß1 increased between 6 and 10 weeks, whereas collagen density increased between 12 and 16 weeks. A negative correlation was observed between liver collagen deposition and serum levels of TGFß1 (r = -0. 48; P<0. 05). CONCLUSION: Serum levels of TGFß1 were inversely proportional to collagen intensity in cirrhotic livers of rats exposed to CCl4, thus suggesting a limited use as biomarker in advanced liver disease.
publishDate 2012
dc.date.none.fl_str_mv 2012-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-28032012000300013
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-28032012000300013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0004-28032012000300013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia e Outras Especialidades - IBEPEGE.
publisher.none.fl_str_mv Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia e Outras Especialidades - IBEPEGE.
dc.source.none.fl_str_mv Arquivos de Gastroenterologia v.49 n.3 2012
reponame:Arquivos de gastroenterologia (Online)
instname:Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia
instacron:IBEPEGE
instname_str Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia
instacron_str IBEPEGE
institution IBEPEGE
reponame_str Arquivos de gastroenterologia (Online)
collection Arquivos de gastroenterologia (Online)
repository.name.fl_str_mv Arquivos de gastroenterologia (Online) - Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia
repository.mail.fl_str_mv ||secretariaarqgastr@hospitaligesp.com.br
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