Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi

Detalhes bibliográficos
Autor(a) principal: Ferreira,Ana Beatriz Monteiro
Data de Publicação: 2019
Outros Autores: Fischer,Ivan Herman, Leite,Luís Garrigós, Padovani,Carlos Roberto, Bueno,César Júnior
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Arquivos do instituto biológico (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572019000100209
Resumo: ABSTRACT: Extracellular enzymes are involved in the fungal pathogenesis in plants. Currently, culture media, data analyses, and data report related to extracellular enzymes produced in vitro conditions are different and therefore, lack standardization. This work aimed to compare the culture media cited on the literature (normal) with the potato-dextrose-agar (PDA) medium combined with a specific compound to produce extracellular enzymes through three soilborne phytopathogenic fungi (F. solani f. sp. passiflorae, S. rolfsii, and R. solani AG-4 HGI), as well as to analyze and report enzyme data based on five different criteria. The assay was randomized, with three factors (culture media, isolates, and enzymes) and six repetitions. The studied enzymes were amylase (AM), carboxymethylcellulase (CMCase), lipase (LP), laccase (LC), catalase (CT), and gelatinase (GT). The normal media detected more enzymes and was more precise compared to the PDA medium plus specific compound. The criteria that calculated the area of the circular crown of AM, CMCase, LP, and LC and measured the intensity (0 = absence, up to 4 = intense) of CT and GT adopting note scale were the best to evaluate and report the results of the enzymes. We suggest the normal media culture to study enzyme production, as well as the criteria mentioned to assess and report the data related to enzyme activities.
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spelling Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungienzymessoilborne phytopathogenic fungimethodologyABSTRACT: Extracellular enzymes are involved in the fungal pathogenesis in plants. Currently, culture media, data analyses, and data report related to extracellular enzymes produced in vitro conditions are different and therefore, lack standardization. This work aimed to compare the culture media cited on the literature (normal) with the potato-dextrose-agar (PDA) medium combined with a specific compound to produce extracellular enzymes through three soilborne phytopathogenic fungi (F. solani f. sp. passiflorae, S. rolfsii, and R. solani AG-4 HGI), as well as to analyze and report enzyme data based on five different criteria. The assay was randomized, with three factors (culture media, isolates, and enzymes) and six repetitions. The studied enzymes were amylase (AM), carboxymethylcellulase (CMCase), lipase (LP), laccase (LC), catalase (CT), and gelatinase (GT). The normal media detected more enzymes and was more precise compared to the PDA medium plus specific compound. The criteria that calculated the area of the circular crown of AM, CMCase, LP, and LC and measured the intensity (0 = absence, up to 4 = intense) of CT and GT adopting note scale were the best to evaluate and report the results of the enzymes. We suggest the normal media culture to study enzyme production, as well as the criteria mentioned to assess and report the data related to enzyme activities.Instituto Biológico2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572019000100209Arquivos do Instituto Biológico v.86 2019reponame:Arquivos do instituto biológico (Online)instname:Instituto Biológico (IB)instacron:IBIO10.1590/1808-1657000592017info:eu-repo/semantics/openAccessFerreira,Ana Beatriz MonteiroFischer,Ivan HermanLeite,Luís GarrigósPadovani,Carlos RobertoBueno,César Júnioreng2019-07-24T00:00:00Zoai:scielo:S1808-16572019000100209Revistahttp://www.biologico.sp.gov.br/arquivos_bio.phphttps://old.scielo.br/oai/scielo-oai.php||arquivos@biologico.sp.gov.br1808-16570020-3653opendoar:2019-07-24T00:00Arquivos do instituto biológico (Online) - Instituto Biológico (IB)false
dc.title.none.fl_str_mv Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi
title Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi
spellingShingle Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi
Ferreira,Ana Beatriz Monteiro
enzymes
soilborne phytopathogenic fungi
methodology
title_short Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi
title_full Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi
title_fullStr Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi
title_full_unstemmed Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi
title_sort Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi
author Ferreira,Ana Beatriz Monteiro
author_facet Ferreira,Ana Beatriz Monteiro
Fischer,Ivan Herman
Leite,Luís Garrigós
Padovani,Carlos Roberto
Bueno,César Júnior
author_role author
author2 Fischer,Ivan Herman
Leite,Luís Garrigós
Padovani,Carlos Roberto
Bueno,César Júnior
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Ferreira,Ana Beatriz Monteiro
Fischer,Ivan Herman
Leite,Luís Garrigós
Padovani,Carlos Roberto
Bueno,César Júnior
dc.subject.por.fl_str_mv enzymes
soilborne phytopathogenic fungi
methodology
topic enzymes
soilborne phytopathogenic fungi
methodology
description ABSTRACT: Extracellular enzymes are involved in the fungal pathogenesis in plants. Currently, culture media, data analyses, and data report related to extracellular enzymes produced in vitro conditions are different and therefore, lack standardization. This work aimed to compare the culture media cited on the literature (normal) with the potato-dextrose-agar (PDA) medium combined with a specific compound to produce extracellular enzymes through three soilborne phytopathogenic fungi (F. solani f. sp. passiflorae, S. rolfsii, and R. solani AG-4 HGI), as well as to analyze and report enzyme data based on five different criteria. The assay was randomized, with three factors (culture media, isolates, and enzymes) and six repetitions. The studied enzymes were amylase (AM), carboxymethylcellulase (CMCase), lipase (LP), laccase (LC), catalase (CT), and gelatinase (GT). The normal media detected more enzymes and was more precise compared to the PDA medium plus specific compound. The criteria that calculated the area of the circular crown of AM, CMCase, LP, and LC and measured the intensity (0 = absence, up to 4 = intense) of CT and GT adopting note scale were the best to evaluate and report the results of the enzymes. We suggest the normal media culture to study enzyme production, as well as the criteria mentioned to assess and report the data related to enzyme activities.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572019000100209
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572019000100209
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1808-1657000592017
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Biológico
publisher.none.fl_str_mv Instituto Biológico
dc.source.none.fl_str_mv Arquivos do Instituto Biológico v.86 2019
reponame:Arquivos do instituto biológico (Online)
instname:Instituto Biológico (IB)
instacron:IBIO
instname_str Instituto Biológico (IB)
instacron_str IBIO
institution IBIO
reponame_str Arquivos do instituto biológico (Online)
collection Arquivos do instituto biológico (Online)
repository.name.fl_str_mv Arquivos do instituto biológico (Online) - Instituto Biológico (IB)
repository.mail.fl_str_mv ||arquivos@biologico.sp.gov.br
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