Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Arquivos do instituto biológico (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572019000100209 |
Resumo: | ABSTRACT: Extracellular enzymes are involved in the fungal pathogenesis in plants. Currently, culture media, data analyses, and data report related to extracellular enzymes produced in vitro conditions are different and therefore, lack standardization. This work aimed to compare the culture media cited on the literature (normal) with the potato-dextrose-agar (PDA) medium combined with a specific compound to produce extracellular enzymes through three soilborne phytopathogenic fungi (F. solani f. sp. passiflorae, S. rolfsii, and R. solani AG-4 HGI), as well as to analyze and report enzyme data based on five different criteria. The assay was randomized, with three factors (culture media, isolates, and enzymes) and six repetitions. The studied enzymes were amylase (AM), carboxymethylcellulase (CMCase), lipase (LP), laccase (LC), catalase (CT), and gelatinase (GT). The normal media detected more enzymes and was more precise compared to the PDA medium plus specific compound. The criteria that calculated the area of the circular crown of AM, CMCase, LP, and LC and measured the intensity (0 = absence, up to 4 = intense) of CT and GT adopting note scale were the best to evaluate and report the results of the enzymes. We suggest the normal media culture to study enzyme production, as well as the criteria mentioned to assess and report the data related to enzyme activities. |
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Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungienzymessoilborne phytopathogenic fungimethodologyABSTRACT: Extracellular enzymes are involved in the fungal pathogenesis in plants. Currently, culture media, data analyses, and data report related to extracellular enzymes produced in vitro conditions are different and therefore, lack standardization. This work aimed to compare the culture media cited on the literature (normal) with the potato-dextrose-agar (PDA) medium combined with a specific compound to produce extracellular enzymes through three soilborne phytopathogenic fungi (F. solani f. sp. passiflorae, S. rolfsii, and R. solani AG-4 HGI), as well as to analyze and report enzyme data based on five different criteria. The assay was randomized, with three factors (culture media, isolates, and enzymes) and six repetitions. The studied enzymes were amylase (AM), carboxymethylcellulase (CMCase), lipase (LP), laccase (LC), catalase (CT), and gelatinase (GT). The normal media detected more enzymes and was more precise compared to the PDA medium plus specific compound. The criteria that calculated the area of the circular crown of AM, CMCase, LP, and LC and measured the intensity (0 = absence, up to 4 = intense) of CT and GT adopting note scale were the best to evaluate and report the results of the enzymes. We suggest the normal media culture to study enzyme production, as well as the criteria mentioned to assess and report the data related to enzyme activities.Instituto Biológico2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572019000100209Arquivos do Instituto Biológico v.86 2019reponame:Arquivos do instituto biológico (Online)instname:Instituto Biológico (IB)instacron:IBIO10.1590/1808-1657000592017info:eu-repo/semantics/openAccessFerreira,Ana Beatriz MonteiroFischer,Ivan HermanLeite,Luís GarrigósPadovani,Carlos RobertoBueno,César Júnioreng2019-07-24T00:00:00Zoai:scielo:S1808-16572019000100209Revistahttp://www.biologico.sp.gov.br/arquivos_bio.phphttps://old.scielo.br/oai/scielo-oai.php||arquivos@biologico.sp.gov.br1808-16570020-3653opendoar:2019-07-24T00:00Arquivos do instituto biológico (Online) - Instituto Biológico (IB)false |
dc.title.none.fl_str_mv |
Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi |
title |
Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi |
spellingShingle |
Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi Ferreira,Ana Beatriz Monteiro enzymes soilborne phytopathogenic fungi methodology |
title_short |
Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi |
title_full |
Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi |
title_fullStr |
Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi |
title_full_unstemmed |
Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi |
title_sort |
Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi |
author |
Ferreira,Ana Beatriz Monteiro |
author_facet |
Ferreira,Ana Beatriz Monteiro Fischer,Ivan Herman Leite,Luís Garrigós Padovani,Carlos Roberto Bueno,César Júnior |
author_role |
author |
author2 |
Fischer,Ivan Herman Leite,Luís Garrigós Padovani,Carlos Roberto Bueno,César Júnior |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Ferreira,Ana Beatriz Monteiro Fischer,Ivan Herman Leite,Luís Garrigós Padovani,Carlos Roberto Bueno,César Júnior |
dc.subject.por.fl_str_mv |
enzymes soilborne phytopathogenic fungi methodology |
topic |
enzymes soilborne phytopathogenic fungi methodology |
description |
ABSTRACT: Extracellular enzymes are involved in the fungal pathogenesis in plants. Currently, culture media, data analyses, and data report related to extracellular enzymes produced in vitro conditions are different and therefore, lack standardization. This work aimed to compare the culture media cited on the literature (normal) with the potato-dextrose-agar (PDA) medium combined with a specific compound to produce extracellular enzymes through three soilborne phytopathogenic fungi (F. solani f. sp. passiflorae, S. rolfsii, and R. solani AG-4 HGI), as well as to analyze and report enzyme data based on five different criteria. The assay was randomized, with three factors (culture media, isolates, and enzymes) and six repetitions. The studied enzymes were amylase (AM), carboxymethylcellulase (CMCase), lipase (LP), laccase (LC), catalase (CT), and gelatinase (GT). The normal media detected more enzymes and was more precise compared to the PDA medium plus specific compound. The criteria that calculated the area of the circular crown of AM, CMCase, LP, and LC and measured the intensity (0 = absence, up to 4 = intense) of CT and GT adopting note scale were the best to evaluate and report the results of the enzymes. We suggest the normal media culture to study enzyme production, as well as the criteria mentioned to assess and report the data related to enzyme activities. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572019000100209 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572019000100209 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1808-1657000592017 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Biológico |
publisher.none.fl_str_mv |
Instituto Biológico |
dc.source.none.fl_str_mv |
Arquivos do Instituto Biológico v.86 2019 reponame:Arquivos do instituto biológico (Online) instname:Instituto Biológico (IB) instacron:IBIO |
instname_str |
Instituto Biológico (IB) |
instacron_str |
IBIO |
institution |
IBIO |
reponame_str |
Arquivos do instituto biológico (Online) |
collection |
Arquivos do instituto biológico (Online) |
repository.name.fl_str_mv |
Arquivos do instituto biológico (Online) - Instituto Biológico (IB) |
repository.mail.fl_str_mv |
||arquivos@biologico.sp.gov.br |
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1754193670355025920 |