Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi

Detalhes bibliográficos
Autor(a) principal: Ferreira, Ana Beatriz Monteiro
Data de Publicação: 2019
Outros Autores: Fischer, Ivan Herman, Leite, Luís Garrigós, Padovani, Carlos Roberto [UNESP], Bueno, César Júnior
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/1808-1657000592017
http://hdl.handle.net/11449/213213
Resumo: Extracellular enzymes are involved in the fungal pathogenesis in plants. Currently, culture media, data analyses, and data report related to extracellular enzymes produced in vitro conditions are different and therefore, lack standardization. This work aimed to compare the culture media cited on the literature (normal) with the potato-dextrose-agar (PDA) medium combined with a specific compound to produce extracellular enzymes through three soilborne phytopathogenic fungi (F. solani f. sp. passiflorae, S. rolfsii, and R. solani AG-4 HGI), as well as to analyze and report enzyme data based on five different criteria. The assay was randomized, with three factors (culture media, isolates, and enzymes) and six repetitions. The studied enzymes were amylase (AM), carboxymethylcellulase (CMCase), lipase (LP), laccase (LC), catalase (CT), and gelatinase (GT). The normal media detected more enzymes and was more precise compared to the PDA medium plus specific compound. The criteria that calculated the area of the circular crown of AM, CMCase, LP, and LC and measured the intensity (0 = absence, up to 4 = intense) of CT and GT adopting note scale were the best to evaluate and report the results of the enzymes. We suggest the normal media culture to study enzyme production, as well as the criteria mentioned to assess and report the data related to enzyme activities.
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spelling Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungiMeios de cultura para detectar e critérios para avaliar e divulgar atividade de enzimas extracelulares produzidas por fungos fitopatogênicosenzymessoilborne phytopathogenic fungimethodologyenzimasfungos fitopatogênicos habitantes de solometodologiaExtracellular enzymes are involved in the fungal pathogenesis in plants. Currently, culture media, data analyses, and data report related to extracellular enzymes produced in vitro conditions are different and therefore, lack standardization. This work aimed to compare the culture media cited on the literature (normal) with the potato-dextrose-agar (PDA) medium combined with a specific compound to produce extracellular enzymes through three soilborne phytopathogenic fungi (F. solani f. sp. passiflorae, S. rolfsii, and R. solani AG-4 HGI), as well as to analyze and report enzyme data based on five different criteria. The assay was randomized, with three factors (culture media, isolates, and enzymes) and six repetitions. The studied enzymes were amylase (AM), carboxymethylcellulase (CMCase), lipase (LP), laccase (LC), catalase (CT), and gelatinase (GT). The normal media detected more enzymes and was more precise compared to the PDA medium plus specific compound. The criteria that calculated the area of the circular crown of AM, CMCase, LP, and LC and measured the intensity (0 = absence, up to 4 = intense) of CT and GT adopting note scale were the best to evaluate and report the results of the enzymes. We suggest the normal media culture to study enzyme production, as well as the criteria mentioned to assess and report the data related to enzyme activities.As enzimas extracelulares estão envolvidas na patogênese de fungos em plantas. Atualmente, não há uma padronização de meios de cultura, formas de analisar e divulgar os dados de enzimas extracelulares produzidas em condições in vitro. Assim, o presente trabalho objetivou comparar os meios de cultura específicos relatados na literatura (normal) com o meio de batata-dextrose-ágar mais adição do substrato específico para produção de enzimas extracelulares por três diferentes fungos fitopatogênicos habitantes de solo (Fusarium solani f. sp. passiflorae, Sclerotium rolfsii e Rhizoctonia solani AG-4 HGI), bem como avaliar os dados das enzimas por cinco critérios diferentes. O delineamento experimental adotado foi o inteiramente ao acaso, em esquema de três fatores (meios, isolados e enzimas), com seis repetições. As enzimas investigadas foram amilase, carboximetilcelulase, lipase, lacase, catalase e gelatinase. Os meios normais detectaram mais enzimas, e essa detecção foi mais precisa em comparação com os meios de batata-dextrose-ágar mais o substrato específico. Os critérios que calcularam a área da coroa circular para as enzimas amilase, carboximetilcelulase, lipase e lacase e adotaram a escala de notas para medir a intensidade (0=ausência até 4=intensa) de catalase e gelatinase foram os melhores para avaliar e divulgar os resultados das enzimas. Assim, sugere-se padronizar os meios normais para estudos de produção de enzimas, bem como os critérios citados para avaliar e divulgar os dados das atividades das referidas enzimas.Instituto BiológicoPRDTA Centro OesteUniversidade Estadual Paulista “Julio de Mesquita Filho”, Instituto de BiociênciasUniversidade Estadual Paulista “Julio de Mesquita Filho”, Instituto de BiociênciasInstituto BiológicoInstituto BiológicoPRDTA Centro OesteUniversidade Estadual Paulista (Unesp)Ferreira, Ana Beatriz MonteiroFischer, Ivan HermanLeite, Luís GarrigósPadovani, Carlos Roberto [UNESP]Bueno, César Júnior2021-07-14T10:51:49Z2021-07-14T10:51:49Z2019-07-29info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article-application/pdfhttp://dx.doi.org/10.1590/1808-1657000592017Arquivos do Instituto Biológico. Instituto Biológico, v. 86, p. -, 2019.0020-36531808-1657http://hdl.handle.net/11449/21321310.1590/1808-1657000592017S1808-16572019000100209S1808-16572019000100209.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArquivos do Instituto Biológicoinfo:eu-repo/semantics/openAccess2024-01-02T06:18:50Zoai:repositorio.unesp.br:11449/213213Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:55:33.619003Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi
Meios de cultura para detectar e critérios para avaliar e divulgar atividade de enzimas extracelulares produzidas por fungos fitopatogênicos
title Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi
spellingShingle Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi
Ferreira, Ana Beatriz Monteiro
enzymes
soilborne phytopathogenic fungi
methodology
enzimas
fungos fitopatogênicos habitantes de solo
metodologia
title_short Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi
title_full Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi
title_fullStr Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi
title_full_unstemmed Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi
title_sort Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi
author Ferreira, Ana Beatriz Monteiro
author_facet Ferreira, Ana Beatriz Monteiro
Fischer, Ivan Herman
Leite, Luís Garrigós
Padovani, Carlos Roberto [UNESP]
Bueno, César Júnior
author_role author
author2 Fischer, Ivan Herman
Leite, Luís Garrigós
Padovani, Carlos Roberto [UNESP]
Bueno, César Júnior
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Instituto Biológico
PRDTA Centro Oeste
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Ferreira, Ana Beatriz Monteiro
Fischer, Ivan Herman
Leite, Luís Garrigós
Padovani, Carlos Roberto [UNESP]
Bueno, César Júnior
dc.subject.por.fl_str_mv enzymes
soilborne phytopathogenic fungi
methodology
enzimas
fungos fitopatogênicos habitantes de solo
metodologia
topic enzymes
soilborne phytopathogenic fungi
methodology
enzimas
fungos fitopatogênicos habitantes de solo
metodologia
description Extracellular enzymes are involved in the fungal pathogenesis in plants. Currently, culture media, data analyses, and data report related to extracellular enzymes produced in vitro conditions are different and therefore, lack standardization. This work aimed to compare the culture media cited on the literature (normal) with the potato-dextrose-agar (PDA) medium combined with a specific compound to produce extracellular enzymes through three soilborne phytopathogenic fungi (F. solani f. sp. passiflorae, S. rolfsii, and R. solani AG-4 HGI), as well as to analyze and report enzyme data based on five different criteria. The assay was randomized, with three factors (culture media, isolates, and enzymes) and six repetitions. The studied enzymes were amylase (AM), carboxymethylcellulase (CMCase), lipase (LP), laccase (LC), catalase (CT), and gelatinase (GT). The normal media detected more enzymes and was more precise compared to the PDA medium plus specific compound. The criteria that calculated the area of the circular crown of AM, CMCase, LP, and LC and measured the intensity (0 = absence, up to 4 = intense) of CT and GT adopting note scale were the best to evaluate and report the results of the enzymes. We suggest the normal media culture to study enzyme production, as well as the criteria mentioned to assess and report the data related to enzyme activities.
publishDate 2019
dc.date.none.fl_str_mv 2019-07-29
2021-07-14T10:51:49Z
2021-07-14T10:51:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1808-1657000592017
Arquivos do Instituto Biológico. Instituto Biológico, v. 86, p. -, 2019.
0020-3653
1808-1657
http://hdl.handle.net/11449/213213
10.1590/1808-1657000592017
S1808-16572019000100209
S1808-16572019000100209.pdf
url http://dx.doi.org/10.1590/1808-1657000592017
http://hdl.handle.net/11449/213213
identifier_str_mv Arquivos do Instituto Biológico. Instituto Biológico, v. 86, p. -, 2019.
0020-3653
1808-1657
10.1590/1808-1657000592017
S1808-16572019000100209
S1808-16572019000100209.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arquivos do Instituto Biológico
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv -
application/pdf
dc.publisher.none.fl_str_mv Instituto Biológico
publisher.none.fl_str_mv Instituto Biológico
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129373746757632

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