Gene expression of MTOR, MURF-1 and MAFBX in wistar rats supplemented with whey proteins

• Autor(a) principal: Macêdo, Marcos Roberto Campos de
• Data de Publicação: 2023
• Outros Autores: Navarro, Antonio Coppi, Marques, Raphael Furtado, Silva, Melaine Mont´Alverne Lawall, Vidal, Flavia Castello Branco, Silva, Alanna Joselle Santiago, Amorim, Carlos Eduardo Neves, Navarro, Francisco
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Revista brasileira de obesidade, nutrição e emagrecimento
• Texto Completo: https://www.rbone.com.br/index.php/rbone/article/view/2151
• topic: Whey Proteins; MTOR; MURF-1; MAFBX; Gastrocnemius; Proteína de suero; MTOR; MURF-1; MAFBX; Gastrocnemio; Proteine del siero di latte; MTOR; MURF-1; MAFBX; Gastrocnemio; Whey Proteins; MTOR; MURF-1; MAFBX; Gastrocnêmio; Whey Proteins; MTOR; MURF-1; MAFBX; Gastrocnêmio

To verify the effect of supplementation of different doses of Whey Proteins for 12 weeks on the gene expression of MTOR, MURF-1, MAFBX and on the alteration of the cellular diameter of the gastrocnemius muscle. Sample: 38 Wistar rats and supplemented for 12 weeks. The white portion of the right gastrocnemius muscle was embedded in Rna Latter solution and stored at -80 ° C and subjected to real-time polymerase chain reaction analysis. The left gastrocnemius muscle was intended for histological analysis. Gene expression of MTOR mRNA had lower statistical significance in the groups of Whey 2, Whey 4, Whey 6 than in the control group, in the order of 100%, 97%, 96%, respectively. Likewise, the gene expression of MURF-1 mRNA had lower statistical significance in the groups of Whey 2, Whey 4, Whey 6 than the control group, in the order of 65%, 75%, 84%, respectively. In line, the gene expression MAFBX mRNA had lower statistical significance in the groups Whey 2, Whey 4, Whey 6 in relation to the control group, in the order of 99%, 97%, 99%, respectively. There was no statistical difference in the cellular area of the gastrocnemius muscle between the groups. Therefore, supplementation of Whey Proteins at doses of 2, 4, 6 g / kg / day for 12 weeks in sedentary animals was not able to increase the gene expression of MTOR mRNA, however, it was able to reduce the gene expression of MURF -1 mRNA, MAFBX mRNA.