EFEITO DA SUPLEMENTAÇÃO DE DIFERENTES DOSES DE WHEY PROTEINS ASSOCIADAS AO TREINAMENTO RESISTIDO POR DOZE SEMANAS SOBRE A EXPRESSÃO GÊNICA DE MTOR, MURF-1 E MAFBX EM RATOS WISTAR MACHOS.

Detalhes bibliográficos
Autor(a) principal: MARQUES, Raphael Furtado
Data de Publicação: 2018
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFMA
Texto Completo: https://tedebc.ufma.br/jspui/handle/tede/tede/3038
Resumo: Objective: Verify the effect of supplementation of 2, 4 and 6g/kg/day doses of Whey proteins associated with the 12 week resistance training in gene expression in the path of synthesis and protein degradation of the skeletal muscle of male Wistar rats. Materials and Methods: A study of 60 male Wistar rats with initial age of 60 days and body mass of 250g to 350g, randomly distributed in eight groups, one being a control and three interventional sedentary, identified by the dosage value of the supplement WP (2, 4 and 6g/kg/day), a resistance training control and three more trained groups and with whey proteins at doses (2, 4 and 6g/kg/day). The protocol lasted 12 weeks with daily supplementation and resistance training three times a week. The gene expressions of MTOR, MURF-1 and MAFBX in the white portion of the gastrocnemius muscle through the Polimerase Chain Reaction Real Time method (PCR-RT), muscle strength every two weeks and gastrocnemius muscle mass and mean area of the muscle cell were evaluated. Results: Gene expression of MTOR mRNA in the gastrocnemius muscle in the TC group was greater than the W2, W4 and W6 groups but did not present a significant statistical difference between groups C, TW2, TW4 and TW6. Gene expression of MURF-1 mRNA was lower in all treatment groups compared to the control group and was not different in the comparison between doses of whey proteins and/or resistance training. The gene expression of MAFBX mRNA in all groups was lower when compared to the sedentary control group. In addition, the gene expression of MAFBX mRNA in the TW6 group was greater than the W2, W4, W6, TC, TW2 and TW4 groups. Muscle strength increased in all trained groups, but whey proteins had a potentiating effect on this result. In addition, relative muscle mass was higher in the trained group and supplemented with 6g/kg/day. Discussion: Whey protein supplementation had a positive effect on the decrease in the expression of proteins related to protein degradation, which could lead to a positive balance of protein synthesis, and resistance training provided greater activation of the synthesis pathway. The lower expression of these markers of protein degradation is an important indication for a positive balance of protein synthesis in relation to protein degradation, since the reduction of the degradation pathways itself may lead to an increase in muscle hypertrophy. Nevertheless, this condition caused an increase in the relative mass of the gastrocnemius in the TW6 group and the muscle cell area did not have significant statistical difference between the groups. Conclusion: Whey proteins at doses of 2, 4 or 6 g / kg / day in sedentary rats did not provoke an increase in MTOR mRNA expression but caused a decrease in the expression of MURF-1 mRNA and MAFBX mRNA. TR increased MTOR mRNA expression, but whey protein supplementation did not potentiate this effect. The dose of 6g / kg / day of whey proteins caused a greater increase in the relative mass of the gastrocnemius muscle in rats that underwent resistance training. In addition, whey proteins had a potentiating effect on the strength of the animals that underwent resistance training.
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spelling NAVARRO, Francisco484.828.209-82http://lattes.cnpq.br/0628582355687773NAVARRO, Antônio Coppi475.316.739-91http://lattes.cnpq.br/7269812755382405NAVARRO, Francisco484.828.209-82http://lattes.cnpq.br/0628582355687773AMORIM, Carlos Eduardo Neveshttp://lattes.cnpq.br/0109987596093206DIBAI FILHO, Almir Vieirahttp://lattes.cnpq.br/1144110354316280VENEROSO, Christiano Eduardohttp://lattes.cnpq.br/0537304422291084NAVARRO, Antonio Coppihttp://lattes.cnpq.br/7269812755382405044.937.273-14http://lattes.cnpq.br/1218329130529941MARQUES, Raphael Furtado2020-02-07T14:01:00Z2018-08-31MARQUES, Raphael Furtado. Efeito da suplementação de diferentes doses de whey proteins associadas ao treinamento resistido por doze semanas sobre a expressão gênica de mtor, murf-1 e mafbx em ratos wistar machos.. 2018. 121 f.. Dissertação( Programa de Pós-Graduacao em Educação Física) - Universidade Federal do Maranhão, São Luís, 2018.https://tedebc.ufma.br/jspui/handle/tede/tede/3038Objective: Verify the effect of supplementation of 2, 4 and 6g/kg/day doses of Whey proteins associated with the 12 week resistance training in gene expression in the path of synthesis and protein degradation of the skeletal muscle of male Wistar rats. Materials and Methods: A study of 60 male Wistar rats with initial age of 60 days and body mass of 250g to 350g, randomly distributed in eight groups, one being a control and three interventional sedentary, identified by the dosage value of the supplement WP (2, 4 and 6g/kg/day), a resistance training control and three more trained groups and with whey proteins at doses (2, 4 and 6g/kg/day). The protocol lasted 12 weeks with daily supplementation and resistance training three times a week. The gene expressions of MTOR, MURF-1 and MAFBX in the white portion of the gastrocnemius muscle through the Polimerase Chain Reaction Real Time method (PCR-RT), muscle strength every two weeks and gastrocnemius muscle mass and mean area of the muscle cell were evaluated. Results: Gene expression of MTOR mRNA in the gastrocnemius muscle in the TC group was greater than the W2, W4 and W6 groups but did not present a significant statistical difference between groups C, TW2, TW4 and TW6. Gene expression of MURF-1 mRNA was lower in all treatment groups compared to the control group and was not different in the comparison between doses of whey proteins and/or resistance training. The gene expression of MAFBX mRNA in all groups was lower when compared to the sedentary control group. In addition, the gene expression of MAFBX mRNA in the TW6 group was greater than the W2, W4, W6, TC, TW2 and TW4 groups. Muscle strength increased in all trained groups, but whey proteins had a potentiating effect on this result. In addition, relative muscle mass was higher in the trained group and supplemented with 6g/kg/day. Discussion: Whey protein supplementation had a positive effect on the decrease in the expression of proteins related to protein degradation, which could lead to a positive balance of protein synthesis, and resistance training provided greater activation of the synthesis pathway. The lower expression of these markers of protein degradation is an important indication for a positive balance of protein synthesis in relation to protein degradation, since the reduction of the degradation pathways itself may lead to an increase in muscle hypertrophy. Nevertheless, this condition caused an increase in the relative mass of the gastrocnemius in the TW6 group and the muscle cell area did not have significant statistical difference between the groups. Conclusion: Whey proteins at doses of 2, 4 or 6 g / kg / day in sedentary rats did not provoke an increase in MTOR mRNA expression but caused a decrease in the expression of MURF-1 mRNA and MAFBX mRNA. TR increased MTOR mRNA expression, but whey protein supplementation did not potentiate this effect. The dose of 6g / kg / day of whey proteins caused a greater increase in the relative mass of the gastrocnemius muscle in rats that underwent resistance training. In addition, whey proteins had a potentiating effect on the strength of the animals that underwent resistance training.Objetivo: Verificar o efeito da suplementação de 2, 4 e 6g/kg/dia doses de Whey proteins associadas ao Treinamento resistido de 12 semanas na expressão gênica em vias de síntese e degradação proteica do músculo esquelético de ratos machos Wistar. Materiais e Métodos: Estudo realizado com 60 ratos machos da linhagem Wistar com idade inicial de 60 dias e massa corporal de 250g a 350g, distribuídos aleatoriamente em oito grupos, sendo um controle e três de intervenção sedentários, identificados pelo valor da dosagem do suplemento WP (2, 4 e 6g/kg/dia) e um controle treinamento resistido com mais três grupos treinados e com whey proteins nas doses (2, 4 e 6g/kg/dia). O protocolo teve duração de 12 semanas com suplementação diária e treinamento resistido três vezes por semana. Foi avaliada a expressão gênica de MTOR, MURF-1 e MAFBX na porção branca do músculo gastrocnêmio através do método Polimerase chain Reaction real time (PCR-RT), a foça muscular a cada duas semanas e a massa do músculo gastrocnêmio e média da área da célula muscular. Resultados: A expressão gênica de MTOR mRNA no músculo gastrocnêmio no grupo TC foi maior do que os grupos W2, W4 e W6 mas não apresentou diferença estatística significativa entre grupos C, TW2, TW4 e TW6. A expressão gênica de MURF-1 mRNA foi menor em todos os grupos de tratamento, comparados ao grupo controle, e não foi diferente na comparação entre as doses de whey proteins e/ou treinamento resistido. A expressão gênica de MAFBX mRNA em todos os grupos foi menor quando comparados ao grupo controle sedentário. Além disso, a expressão gênica de MAFBX mRNA no grupo TW6, foi maior do que os grupos W2, W4, W6, TC, TW2 e TW4. A força muscular aumentou em todos os grupos treinados, porém a whey proteins teve efeito potencializador nesse resultado. Além disso, a massa muscular relativa foi maior no grupo treinado e suplementado com 6g/kg/dia. Discussão: A suplementação com whey proteins teve efeito positivo sobre a diminuição da expressão de proteínas relacionadas à degradação proteica, o que pode ocasionar em balanço positivo da síntese proteica, e o treinamento resistido proporcionou maior ativação da via de síntese. A menor expressão desses marcadores de degração proteica é um importate indicativo para um balanço positivo da síntese proteica em relação à degradação proteica, haja vista que a própria diminuição das vias de degradação pode provocar um aumento na hipertrofia muscular. Apesar disso, essa condição provocou aumento da massa da relativa do gastrocnêmio no grupo TW6 e a área da célula muscular não teve diferença significativa estatística entre os gupos. Conclusão: Whey proteins com doses de 2, 4 ou 6 g/kg/dia em ratos sedentários não provocou aumento na expressão de MTOR mRNA mas provocou diminuição na expressão de MURF-1 mRNA e MAFBX mRNA. O TR aumentou a expressão de MTOR mRNA, porém a suplementação de whey proteins não potencializou esse efeito. A dose de 6g/kg/dia de whey proteins provocou maior aumento na massa relativa do músculo gastrocnêmio em ratos que realizaram treinamento resistido. Ademais, whey proteins teve efeito potencializador no aumento da força dos animais que realizaram treinamento resistido.Submitted by Maria Aparecida (cidazen@gmail.com) on 2020-02-07T14:01:00Z No. of bitstreams: 1 Raphael F. Marques.pdf: 2335229 bytes, checksum: c909ba5c99efa0d938833fda2efd850b (MD5)Made available in DSpace on 2020-02-07T14:01:00Z (GMT). No. of bitstreams: 1 Raphael F. Marques.pdf: 2335229 bytes, checksum: c909ba5c99efa0d938833fda2efd850b (MD5) Previous issue date: 2018-08-31CAPESapplication/pdfporUniversidade Federal do MaranhãoPROGRAMA DE PÓS-GRADUACAO EM EDUCAÇÃO FÍSICAUFMABrasilDEPARTAMENTO DE EDUCAÇÃO FÍSICA/CCBSWhey Proteins;Treinamento Resistido;MTOR;MUFR-1;MAFBXWhey Proteins;Resistance Training;MTOR;MUFR-1;MAFBXEducação FísicaEFEITO DA SUPLEMENTAÇÃO DE DIFERENTES DOSES DE WHEY PROTEINS ASSOCIADAS AO TREINAMENTO RESISTIDO POR DOZE SEMANAS SOBRE A EXPRESSÃO GÊNICA DE MTOR, MURF-1 E MAFBX EM RATOS WISTAR MACHOS.EFFECT OF THE SUPPLEMENTATION OF DIFFERENT ASSOCIATED WHEY PROTEINS THE TRAINING RESISTED FOR TWELVE WEEKS ON THE GENE EXPRESSION OF MTOR, MURF-1 AND MAFBX IN MALE WISTAR RATS.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFMAinstname:Universidade Federal do Maranhão (UFMA)instacron:UFMAORIGINALRaphael F. Marques.pdfRaphael F. 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dc.title.por.fl_str_mv EFEITO DA SUPLEMENTAÇÃO DE DIFERENTES DOSES DE WHEY PROTEINS ASSOCIADAS AO TREINAMENTO RESISTIDO POR DOZE SEMANAS SOBRE A EXPRESSÃO GÊNICA DE MTOR, MURF-1 E MAFBX EM RATOS WISTAR MACHOS.
dc.title.alternative.eng.fl_str_mv EFFECT OF THE SUPPLEMENTATION OF DIFFERENT ASSOCIATED WHEY PROTEINS THE TRAINING RESISTED FOR TWELVE WEEKS ON THE GENE EXPRESSION OF MTOR, MURF-1 AND MAFBX IN MALE WISTAR RATS.
title EFEITO DA SUPLEMENTAÇÃO DE DIFERENTES DOSES DE WHEY PROTEINS ASSOCIADAS AO TREINAMENTO RESISTIDO POR DOZE SEMANAS SOBRE A EXPRESSÃO GÊNICA DE MTOR, MURF-1 E MAFBX EM RATOS WISTAR MACHOS.
spellingShingle EFEITO DA SUPLEMENTAÇÃO DE DIFERENTES DOSES DE WHEY PROTEINS ASSOCIADAS AO TREINAMENTO RESISTIDO POR DOZE SEMANAS SOBRE A EXPRESSÃO GÊNICA DE MTOR, MURF-1 E MAFBX EM RATOS WISTAR MACHOS.
MARQUES, Raphael Furtado
Whey Proteins;
Treinamento Resistido;
MTOR;
MUFR-1;
MAFBX
Whey Proteins;
Resistance Training;
MTOR;
MUFR-1;
MAFBX
Educação Física
title_short EFEITO DA SUPLEMENTAÇÃO DE DIFERENTES DOSES DE WHEY PROTEINS ASSOCIADAS AO TREINAMENTO RESISTIDO POR DOZE SEMANAS SOBRE A EXPRESSÃO GÊNICA DE MTOR, MURF-1 E MAFBX EM RATOS WISTAR MACHOS.
title_full EFEITO DA SUPLEMENTAÇÃO DE DIFERENTES DOSES DE WHEY PROTEINS ASSOCIADAS AO TREINAMENTO RESISTIDO POR DOZE SEMANAS SOBRE A EXPRESSÃO GÊNICA DE MTOR, MURF-1 E MAFBX EM RATOS WISTAR MACHOS.
title_fullStr EFEITO DA SUPLEMENTAÇÃO DE DIFERENTES DOSES DE WHEY PROTEINS ASSOCIADAS AO TREINAMENTO RESISTIDO POR DOZE SEMANAS SOBRE A EXPRESSÃO GÊNICA DE MTOR, MURF-1 E MAFBX EM RATOS WISTAR MACHOS.
title_full_unstemmed EFEITO DA SUPLEMENTAÇÃO DE DIFERENTES DOSES DE WHEY PROTEINS ASSOCIADAS AO TREINAMENTO RESISTIDO POR DOZE SEMANAS SOBRE A EXPRESSÃO GÊNICA DE MTOR, MURF-1 E MAFBX EM RATOS WISTAR MACHOS.
title_sort EFEITO DA SUPLEMENTAÇÃO DE DIFERENTES DOSES DE WHEY PROTEINS ASSOCIADAS AO TREINAMENTO RESISTIDO POR DOZE SEMANAS SOBRE A EXPRESSÃO GÊNICA DE MTOR, MURF-1 E MAFBX EM RATOS WISTAR MACHOS.
author MARQUES, Raphael Furtado
author_facet MARQUES, Raphael Furtado
author_role author
dc.contributor.advisor1.fl_str_mv NAVARRO, Francisco
dc.contributor.advisor1ID.fl_str_mv 484.828.209-82
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/0628582355687773
dc.contributor.advisor-co1.fl_str_mv NAVARRO, Antônio Coppi
dc.contributor.advisor-co1ID.fl_str_mv 475.316.739-91
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/7269812755382405
dc.contributor.referee1.fl_str_mv NAVARRO, Francisco
dc.contributor.referee1ID.fl_str_mv 484.828.209-82
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/0628582355687773
dc.contributor.referee2.fl_str_mv AMORIM, Carlos Eduardo Neves
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/0109987596093206
dc.contributor.referee3.fl_str_mv DIBAI FILHO, Almir Vieira
dc.contributor.referee3Lattes.fl_str_mv http://lattes.cnpq.br/1144110354316280
dc.contributor.referee4.fl_str_mv VENEROSO, Christiano Eduardo
dc.contributor.referee4Lattes.fl_str_mv http://lattes.cnpq.br/0537304422291084
dc.contributor.referee5.fl_str_mv NAVARRO, Antonio Coppi
dc.contributor.referee5Lattes.fl_str_mv http://lattes.cnpq.br/7269812755382405
dc.contributor.authorID.fl_str_mv 044.937.273-14
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/1218329130529941
dc.contributor.author.fl_str_mv MARQUES, Raphael Furtado
contributor_str_mv NAVARRO, Francisco
NAVARRO, Antônio Coppi
NAVARRO, Francisco
AMORIM, Carlos Eduardo Neves
DIBAI FILHO, Almir Vieira
VENEROSO, Christiano Eduardo
NAVARRO, Antonio Coppi
dc.subject.por.fl_str_mv Whey Proteins;
Treinamento Resistido;
MTOR;
MUFR-1;
MAFBX
topic Whey Proteins;
Treinamento Resistido;
MTOR;
MUFR-1;
MAFBX
Whey Proteins;
Resistance Training;
MTOR;
MUFR-1;
MAFBX
Educação Física
dc.subject.eng.fl_str_mv Whey Proteins;
Resistance Training;
MTOR;
MUFR-1;
MAFBX
dc.subject.cnpq.fl_str_mv Educação Física
description Objective: Verify the effect of supplementation of 2, 4 and 6g/kg/day doses of Whey proteins associated with the 12 week resistance training in gene expression in the path of synthesis and protein degradation of the skeletal muscle of male Wistar rats. Materials and Methods: A study of 60 male Wistar rats with initial age of 60 days and body mass of 250g to 350g, randomly distributed in eight groups, one being a control and three interventional sedentary, identified by the dosage value of the supplement WP (2, 4 and 6g/kg/day), a resistance training control and three more trained groups and with whey proteins at doses (2, 4 and 6g/kg/day). The protocol lasted 12 weeks with daily supplementation and resistance training three times a week. The gene expressions of MTOR, MURF-1 and MAFBX in the white portion of the gastrocnemius muscle through the Polimerase Chain Reaction Real Time method (PCR-RT), muscle strength every two weeks and gastrocnemius muscle mass and mean area of the muscle cell were evaluated. Results: Gene expression of MTOR mRNA in the gastrocnemius muscle in the TC group was greater than the W2, W4 and W6 groups but did not present a significant statistical difference between groups C, TW2, TW4 and TW6. Gene expression of MURF-1 mRNA was lower in all treatment groups compared to the control group and was not different in the comparison between doses of whey proteins and/or resistance training. The gene expression of MAFBX mRNA in all groups was lower when compared to the sedentary control group. In addition, the gene expression of MAFBX mRNA in the TW6 group was greater than the W2, W4, W6, TC, TW2 and TW4 groups. Muscle strength increased in all trained groups, but whey proteins had a potentiating effect on this result. In addition, relative muscle mass was higher in the trained group and supplemented with 6g/kg/day. Discussion: Whey protein supplementation had a positive effect on the decrease in the expression of proteins related to protein degradation, which could lead to a positive balance of protein synthesis, and resistance training provided greater activation of the synthesis pathway. The lower expression of these markers of protein degradation is an important indication for a positive balance of protein synthesis in relation to protein degradation, since the reduction of the degradation pathways itself may lead to an increase in muscle hypertrophy. Nevertheless, this condition caused an increase in the relative mass of the gastrocnemius in the TW6 group and the muscle cell area did not have significant statistical difference between the groups. Conclusion: Whey proteins at doses of 2, 4 or 6 g / kg / day in sedentary rats did not provoke an increase in MTOR mRNA expression but caused a decrease in the expression of MURF-1 mRNA and MAFBX mRNA. TR increased MTOR mRNA expression, but whey protein supplementation did not potentiate this effect. The dose of 6g / kg / day of whey proteins caused a greater increase in the relative mass of the gastrocnemius muscle in rats that underwent resistance training. In addition, whey proteins had a potentiating effect on the strength of the animals that underwent resistance training.
publishDate 2018
dc.date.issued.fl_str_mv 2018-08-31
dc.date.accessioned.fl_str_mv 2020-02-07T14:01:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.citation.fl_str_mv MARQUES, Raphael Furtado. Efeito da suplementação de diferentes doses de whey proteins associadas ao treinamento resistido por doze semanas sobre a expressão gênica de mtor, murf-1 e mafbx em ratos wistar machos.. 2018. 121 f.. Dissertação( Programa de Pós-Graduacao em Educação Física) - Universidade Federal do Maranhão, São Luís, 2018.
dc.identifier.uri.fl_str_mv https://tedebc.ufma.br/jspui/handle/tede/tede/3038
identifier_str_mv MARQUES, Raphael Furtado. Efeito da suplementação de diferentes doses de whey proteins associadas ao treinamento resistido por doze semanas sobre a expressão gênica de mtor, murf-1 e mafbx em ratos wistar machos.. 2018. 121 f.. Dissertação( Programa de Pós-Graduacao em Educação Física) - Universidade Federal do Maranhão, São Luís, 2018.
url https://tedebc.ufma.br/jspui/handle/tede/tede/3038
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Universidade Federal do Maranhão
dc.publisher.program.fl_str_mv PROGRAMA DE PÓS-GRADUACAO EM EDUCAÇÃO FÍSICA
dc.publisher.initials.fl_str_mv UFMA
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv DEPARTAMENTO DE EDUCAÇÃO FÍSICA/CCBS
publisher.none.fl_str_mv Universidade Federal do Maranhão
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UFMA
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instacron:UFMA
instname_str Universidade Federal do Maranhão (UFMA)
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reponame_str Biblioteca Digital de Teses e Dissertações da UFMA
collection Biblioteca Digital de Teses e Dissertações da UFMA
bitstream.url.fl_str_mv http://tedebc.ufma.br:8080/bitstream/tede/3038/2/Raphael+F.+Marques.pdf
http://tedebc.ufma.br:8080/bitstream/tede/3038/1/license.txt
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repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da UFMA - Universidade Federal do Maranhão (UFMA)
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