In vitro culture of zygotic embryos and seeds of Caesalpinia ferrea Martius

Detalhes bibliográficos
Autor(a) principal: Silva,Daniel da
Data de Publicação: 2018
Outros Autores: Imakawa,Angela Maria, Costa,Suely de Souza, Sampaio,Paulo de Tarso Barbosa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Hoehnea
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2236-89062018000400663
Resumo: ABSTRACT The aim of this study was to evaluate the in vitro germination of zygotic embryos and seeds of Caesalpinia ferrea Martius and the morphogenetic responses of the explants to different concentrations of growth regulators. Seeds and zygotic embryos were inoculated in MS culture medium and kept in a growth room at a temperature of 25 ± 2 ºC for 16 hours of photoperiod for 30 days. The seeds had a higher in vitro germination rate than the explants from zygotic embryos. However, zygotic embryos in MS medium supplemented with 0.9 mg L-1 BAP had the highest percentage of regeneration (50%), number of shoots (3.25), buds (2.85) and leaves (3.15), multiplication rate (27.75), and length of shoots (1.96 cm). The in vitro culture of zygotic embryos and seeds made possible the multiplication of a higher number of healthy seedlings. Thus, it can be used as an alternative technique for the propagation of this species.
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spelling In vitro culture of zygotic embryos and seeds of Caesalpinia ferrea Martiusauxinscytokininsironwoodmorphogenesistissue cultureABSTRACT The aim of this study was to evaluate the in vitro germination of zygotic embryos and seeds of Caesalpinia ferrea Martius and the morphogenetic responses of the explants to different concentrations of growth regulators. Seeds and zygotic embryos were inoculated in MS culture medium and kept in a growth room at a temperature of 25 ± 2 ºC for 16 hours of photoperiod for 30 days. The seeds had a higher in vitro germination rate than the explants from zygotic embryos. However, zygotic embryos in MS medium supplemented with 0.9 mg L-1 BAP had the highest percentage of regeneration (50%), number of shoots (3.25), buds (2.85) and leaves (3.15), multiplication rate (27.75), and length of shoots (1.96 cm). The in vitro culture of zygotic embryos and seeds made possible the multiplication of a higher number of healthy seedlings. Thus, it can be used as an alternative technique for the propagation of this species.Instituto de Pesquisas Ambientais2018-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2236-89062018000400663Hoehnea v.45 n.4 2018reponame:Hoehneainstname:Instituto de Botânica (IBT)instacron:IBT10.1590/2236-8906-65/2018info:eu-repo/semantics/openAccessSilva,Daniel daImakawa,Angela MariaCosta,Suely de SouzaSampaio,Paulo de Tarso Barbosaeng2019-01-14T00:00:00Zoai:scielo:S2236-89062018000400663Revistahttp://www.ibot.sp.gov.br/publicacoes/hoehnea/sobre_hoehnea.phpPUBhttps://old.scielo.br/oai/scielo-oai.php||hoehneaibt@gmail.com2236-89060073-2877opendoar:2019-01-14T00:00Hoehnea - Instituto de Botânica (IBT)false
dc.title.none.fl_str_mv In vitro culture of zygotic embryos and seeds of Caesalpinia ferrea Martius
title In vitro culture of zygotic embryos and seeds of Caesalpinia ferrea Martius
spellingShingle In vitro culture of zygotic embryos and seeds of Caesalpinia ferrea Martius
Silva,Daniel da
auxins
cytokinins
ironwood
morphogenesis
tissue culture
title_short In vitro culture of zygotic embryos and seeds of Caesalpinia ferrea Martius
title_full In vitro culture of zygotic embryos and seeds of Caesalpinia ferrea Martius
title_fullStr In vitro culture of zygotic embryos and seeds of Caesalpinia ferrea Martius
title_full_unstemmed In vitro culture of zygotic embryos and seeds of Caesalpinia ferrea Martius
title_sort In vitro culture of zygotic embryos and seeds of Caesalpinia ferrea Martius
author Silva,Daniel da
author_facet Silva,Daniel da
Imakawa,Angela Maria
Costa,Suely de Souza
Sampaio,Paulo de Tarso Barbosa
author_role author
author2 Imakawa,Angela Maria
Costa,Suely de Souza
Sampaio,Paulo de Tarso Barbosa
author2_role author
author
author
dc.contributor.author.fl_str_mv Silva,Daniel da
Imakawa,Angela Maria
Costa,Suely de Souza
Sampaio,Paulo de Tarso Barbosa
dc.subject.por.fl_str_mv auxins
cytokinins
ironwood
morphogenesis
tissue culture
topic auxins
cytokinins
ironwood
morphogenesis
tissue culture
description ABSTRACT The aim of this study was to evaluate the in vitro germination of zygotic embryos and seeds of Caesalpinia ferrea Martius and the morphogenetic responses of the explants to different concentrations of growth regulators. Seeds and zygotic embryos were inoculated in MS culture medium and kept in a growth room at a temperature of 25 ± 2 ºC for 16 hours of photoperiod for 30 days. The seeds had a higher in vitro germination rate than the explants from zygotic embryos. However, zygotic embryos in MS medium supplemented with 0.9 mg L-1 BAP had the highest percentage of regeneration (50%), number of shoots (3.25), buds (2.85) and leaves (3.15), multiplication rate (27.75), and length of shoots (1.96 cm). The in vitro culture of zygotic embryos and seeds made possible the multiplication of a higher number of healthy seedlings. Thus, it can be used as an alternative technique for the propagation of this species.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2236-89062018000400663
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2236-89062018000400663
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/2236-8906-65/2018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Pesquisas Ambientais
publisher.none.fl_str_mv Instituto de Pesquisas Ambientais
dc.source.none.fl_str_mv Hoehnea v.45 n.4 2018
reponame:Hoehnea
instname:Instituto de Botânica (IBT)
instacron:IBT
instname_str Instituto de Botânica (IBT)
instacron_str IBT
institution IBT
reponame_str Hoehnea
collection Hoehnea
repository.name.fl_str_mv Hoehnea - Instituto de Botânica (IBT)
repository.mail.fl_str_mv ||hoehneaibt@gmail.com
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