Somatic embryogenesis, organogenesis and callus growth kinetics of flax (Linum usitatissimum L.)
Autor(a) principal: | |
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Data de Publicação: | 1996 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/3005 |
Resumo: | The effects of plant growth regulators (PGR) on calli induction, morphogenesis and somatic embryogenesis of flax were studied. The organogenic and callus formation capacity were assessed for different types of source explants. Root and shoot explants were equally good material for calli production but the former produced calli without shoot regeneration capacity. Under the experimental conditions tested, 2,4-dichlorophenoxyacetic acid (2,4-D) + zeatin (ZEA) was the most efficient PGR combination on calli induction and biomass production. The calli were green but with no rhizogenic capacity. On the contrary, and at similar concentrations, indole-3-butyric acid (IBA) + kinetin (KIN) induced white or pale green friable calli with a good root regeneration capacity (60%). A factorial experiment with different combinations of 2,4-D + ZEA + gibberellic acid (GA3) levels revealed that the direction of explant differentiation was determined by specific PGR interactions and concentrations. The results from these experiments revealed that the morphogenetic pathway (shoot versus root differentiation) can be manipulated on flax explants by raising the 2,4-D level from 0.05 to 3.2 mg l-1 in the induction medium. The induction and development of somatic embryos from flax explants was possible in a range of 2,4-D+ZEA concentrations surrounding 0.4 mg l-1 2,4-D and 1.6 mg l-1 ZEA, the most efficient phytohormonal combination. |
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Somatic embryogenesis, organogenesis and callus growth kinetics of flax (Linum usitatissimum L.)AuxinsCytokininsIn vitro cultureLinum usitatissimumSomatic embryosThe effects of plant growth regulators (PGR) on calli induction, morphogenesis and somatic embryogenesis of flax were studied. The organogenic and callus formation capacity were assessed for different types of source explants. Root and shoot explants were equally good material for calli production but the former produced calli without shoot regeneration capacity. Under the experimental conditions tested, 2,4-dichlorophenoxyacetic acid (2,4-D) + zeatin (ZEA) was the most efficient PGR combination on calli induction and biomass production. The calli were green but with no rhizogenic capacity. On the contrary, and at similar concentrations, indole-3-butyric acid (IBA) + kinetin (KIN) induced white or pale green friable calli with a good root regeneration capacity (60%). A factorial experiment with different combinations of 2,4-D + ZEA + gibberellic acid (GA3) levels revealed that the direction of explant differentiation was determined by specific PGR interactions and concentrations. The results from these experiments revealed that the morphogenetic pathway (shoot versus root differentiation) can be manipulated on flax explants by raising the 2,4-D level from 0.05 to 3.2 mg l-1 in the induction medium. The induction and development of somatic embryos from flax explants was possible in a range of 2,4-D+ZEA concentrations surrounding 0.4 mg l-1 2,4-D and 1.6 mg l-1 ZEA, the most efficient phytohormonal combination.Junta Nacional de Investigação Científica e Tecnológia (JNICT) - BD/2771/93.KluwerUniversidade do MinhoCunha, AnaFerreira, Manuel Fernandes19961996-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/3005eng"Plant Cell, Tissue and Organ Culture". ISSN 0167-6857. 47:1 (1996) 1-8.0167-6857http://www.ingentaconnect.com/content/klu/ticu/1996/00000047/00000001;jsessionid=atlp2ngbqb52q.victoriainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T06:32:47Zoai:repositorium.sdum.uminho.pt:1822/3005Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T06:32:47Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Somatic embryogenesis, organogenesis and callus growth kinetics of flax (Linum usitatissimum L.) |
title |
Somatic embryogenesis, organogenesis and callus growth kinetics of flax (Linum usitatissimum L.) |
spellingShingle |
Somatic embryogenesis, organogenesis and callus growth kinetics of flax (Linum usitatissimum L.) Cunha, Ana Auxins Cytokinins In vitro culture Linum usitatissimum Somatic embryos |
title_short |
Somatic embryogenesis, organogenesis and callus growth kinetics of flax (Linum usitatissimum L.) |
title_full |
Somatic embryogenesis, organogenesis and callus growth kinetics of flax (Linum usitatissimum L.) |
title_fullStr |
Somatic embryogenesis, organogenesis and callus growth kinetics of flax (Linum usitatissimum L.) |
title_full_unstemmed |
Somatic embryogenesis, organogenesis and callus growth kinetics of flax (Linum usitatissimum L.) |
title_sort |
Somatic embryogenesis, organogenesis and callus growth kinetics of flax (Linum usitatissimum L.) |
author |
Cunha, Ana |
author_facet |
Cunha, Ana Ferreira, Manuel Fernandes |
author_role |
author |
author2 |
Ferreira, Manuel Fernandes |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Cunha, Ana Ferreira, Manuel Fernandes |
dc.subject.por.fl_str_mv |
Auxins Cytokinins In vitro culture Linum usitatissimum Somatic embryos |
topic |
Auxins Cytokinins In vitro culture Linum usitatissimum Somatic embryos |
description |
The effects of plant growth regulators (PGR) on calli induction, morphogenesis and somatic embryogenesis of flax were studied. The organogenic and callus formation capacity were assessed for different types of source explants. Root and shoot explants were equally good material for calli production but the former produced calli without shoot regeneration capacity. Under the experimental conditions tested, 2,4-dichlorophenoxyacetic acid (2,4-D) + zeatin (ZEA) was the most efficient PGR combination on calli induction and biomass production. The calli were green but with no rhizogenic capacity. On the contrary, and at similar concentrations, indole-3-butyric acid (IBA) + kinetin (KIN) induced white or pale green friable calli with a good root regeneration capacity (60%). A factorial experiment with different combinations of 2,4-D + ZEA + gibberellic acid (GA3) levels revealed that the direction of explant differentiation was determined by specific PGR interactions and concentrations. The results from these experiments revealed that the morphogenetic pathway (shoot versus root differentiation) can be manipulated on flax explants by raising the 2,4-D level from 0.05 to 3.2 mg l-1 in the induction medium. The induction and development of somatic embryos from flax explants was possible in a range of 2,4-D+ZEA concentrations surrounding 0.4 mg l-1 2,4-D and 1.6 mg l-1 ZEA, the most efficient phytohormonal combination. |
publishDate |
1996 |
dc.date.none.fl_str_mv |
1996 1996-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/3005 |
url |
http://hdl.handle.net/1822/3005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Plant Cell, Tissue and Organ Culture". ISSN 0167-6857. 47:1 (1996) 1-8. 0167-6857 http://www.ingentaconnect.com/content/klu/ticu/1996/00000047/00000001;jsessionid=atlp2ngbqb52q.victoria |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Kluwer |
publisher.none.fl_str_mv |
Kluwer |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817545008913317888 |