Callus induction and betacyanin quantification by HPLC/MS-MS in Alternanthera brasiliana (L.) Kuntze

Detalhes bibliográficos
Autor(a) principal: Reis,Andressa
Data de Publicação: 2017
Outros Autores: Kleinowski,Alítcia Moraes, Klein,Fátima Rosane Schuquel, Souza,Renata Trevizan Telles de, Amarante,Luciano do, Braga,Eugenia Jacira Bolacel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Hoehnea
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2236-89062017000100090
Resumo: ABSTRACT The objective of this study was to establish a protocol for callus induction and betacyanin production in plants of Alternanthera brasiliana. Explants of A. brasiliana and five combinations of cytokinin and auxin were used for callus induction. Calli were transferred to a Betacyanin Induction Medium (MIB), composed of MS, with 0.5 mg L-1 of thidiazuron (TDZ) and 1 mg L-1 of naphthaleneacetic acid (NAA), and kept in the light for 45 days. The aspect and intensity of pigments were assessed and total betacyanins were quantified in high performance liquid chromatography (HPLC). The combination of internodal segments and a medium containing equilibrium concentrations of auxins and cytokinins was the most efficient metod to induce calli and increased production of betacyanins. The presence of amaranthine in calli of A. brasiliana justifies its medical use and the consequent need for future studies for the large-scale production of this molecule.
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spelling Callus induction and betacyanin quantification by HPLC/MS-MS in Alternanthera brasiliana (L.) Kuntzein vitro growthmedicinal plantsphyto-regulatorspigmentsABSTRACT The objective of this study was to establish a protocol for callus induction and betacyanin production in plants of Alternanthera brasiliana. Explants of A. brasiliana and five combinations of cytokinin and auxin were used for callus induction. Calli were transferred to a Betacyanin Induction Medium (MIB), composed of MS, with 0.5 mg L-1 of thidiazuron (TDZ) and 1 mg L-1 of naphthaleneacetic acid (NAA), and kept in the light for 45 days. The aspect and intensity of pigments were assessed and total betacyanins were quantified in high performance liquid chromatography (HPLC). The combination of internodal segments and a medium containing equilibrium concentrations of auxins and cytokinins was the most efficient metod to induce calli and increased production of betacyanins. The presence of amaranthine in calli of A. brasiliana justifies its medical use and the consequent need for future studies for the large-scale production of this molecule.Instituto de Pesquisas Ambientais2017-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2236-89062017000100090Hoehnea v.44 n.1 2017reponame:Hoehneainstname:Instituto de Botânica (IBT)instacron:IBT10.1590/2236-8906-75/2016info:eu-repo/semantics/openAccessReis,AndressaKleinowski,Alítcia MoraesKlein,Fátima Rosane SchuquelSouza,Renata Trevizan Telles deAmarante,Luciano doBraga,Eugenia Jacira Bolaceleng2017-03-17T00:00:00Zoai:scielo:S2236-89062017000100090Revistahttp://www.ibot.sp.gov.br/publicacoes/hoehnea/sobre_hoehnea.phpPUBhttps://old.scielo.br/oai/scielo-oai.php||hoehneaibt@gmail.com2236-89060073-2877opendoar:2017-03-17T00:00Hoehnea - Instituto de Botânica (IBT)false
dc.title.none.fl_str_mv Callus induction and betacyanin quantification by HPLC/MS-MS in Alternanthera brasiliana (L.) Kuntze
title Callus induction and betacyanin quantification by HPLC/MS-MS in Alternanthera brasiliana (L.) Kuntze
spellingShingle Callus induction and betacyanin quantification by HPLC/MS-MS in Alternanthera brasiliana (L.) Kuntze
Reis,Andressa
in vitro growth
medicinal plants
phyto-regulators
pigments
title_short Callus induction and betacyanin quantification by HPLC/MS-MS in Alternanthera brasiliana (L.) Kuntze
title_full Callus induction and betacyanin quantification by HPLC/MS-MS in Alternanthera brasiliana (L.) Kuntze
title_fullStr Callus induction and betacyanin quantification by HPLC/MS-MS in Alternanthera brasiliana (L.) Kuntze
title_full_unstemmed Callus induction and betacyanin quantification by HPLC/MS-MS in Alternanthera brasiliana (L.) Kuntze
title_sort Callus induction and betacyanin quantification by HPLC/MS-MS in Alternanthera brasiliana (L.) Kuntze
author Reis,Andressa
author_facet Reis,Andressa
Kleinowski,Alítcia Moraes
Klein,Fátima Rosane Schuquel
Souza,Renata Trevizan Telles de
Amarante,Luciano do
Braga,Eugenia Jacira Bolacel
author_role author
author2 Kleinowski,Alítcia Moraes
Klein,Fátima Rosane Schuquel
Souza,Renata Trevizan Telles de
Amarante,Luciano do
Braga,Eugenia Jacira Bolacel
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Reis,Andressa
Kleinowski,Alítcia Moraes
Klein,Fátima Rosane Schuquel
Souza,Renata Trevizan Telles de
Amarante,Luciano do
Braga,Eugenia Jacira Bolacel
dc.subject.por.fl_str_mv in vitro growth
medicinal plants
phyto-regulators
pigments
topic in vitro growth
medicinal plants
phyto-regulators
pigments
description ABSTRACT The objective of this study was to establish a protocol for callus induction and betacyanin production in plants of Alternanthera brasiliana. Explants of A. brasiliana and five combinations of cytokinin and auxin were used for callus induction. Calli were transferred to a Betacyanin Induction Medium (MIB), composed of MS, with 0.5 mg L-1 of thidiazuron (TDZ) and 1 mg L-1 of naphthaleneacetic acid (NAA), and kept in the light for 45 days. The aspect and intensity of pigments were assessed and total betacyanins were quantified in high performance liquid chromatography (HPLC). The combination of internodal segments and a medium containing equilibrium concentrations of auxins and cytokinins was the most efficient metod to induce calli and increased production of betacyanins. The presence of amaranthine in calli of A. brasiliana justifies its medical use and the consequent need for future studies for the large-scale production of this molecule.
publishDate 2017
dc.date.none.fl_str_mv 2017-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2236-89062017000100090
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2236-89062017000100090
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/2236-8906-75/2016
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Pesquisas Ambientais
publisher.none.fl_str_mv Instituto de Pesquisas Ambientais
dc.source.none.fl_str_mv Hoehnea v.44 n.1 2017
reponame:Hoehnea
instname:Instituto de Botânica (IBT)
instacron:IBT
instname_str Instituto de Botânica (IBT)
instacron_str IBT
institution IBT
reponame_str Hoehnea
collection Hoehnea
repository.name.fl_str_mv Hoehnea - Instituto de Botânica (IBT)
repository.mail.fl_str_mv ||hoehneaibt@gmail.com
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