Induction and growth pattern of callus from Piper permucronatum leaves

Detalhes bibliográficos
Autor(a) principal: SANTOS,M.R.A.
Data de Publicação: 2016
Outros Autores: GUIMARÃES,M.C.M., PAZ,E.S., MAGALHÃES,G.M.O., SOUZA,C.A., SMOZINSKI,C.V., NOGUEIRA,W.O.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista brasileira de plantas medicinais (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-05722016000100142
Resumo: ABSTRACT Piper permucronatum is a perennial shrub, a medicinal plant native to the Amazon Rainforest. Traditionally, the tea of its leaves is used to combat menstrual and intestinal cramps, stomach pain, digestive problems, diarrhea, hemorrhage, and nausea. Its leaf’s essential oil is effective against Aedes aegypti larvae; its flavones and flavanones have a fungicidal effect against Clamidosporium cladosporioides and C. sphaerospermum; its hexanic extract is effective against Leishmania amazonensis. The objective of this study was to provide a protocol for callus induction from P. permucronatum leaves and an identification of the callus growth pattern, focusing on the deceleration phase, when the callus cells must be subcultured into liquid medium in order to produce a cell suspension cultures. Leaf explants were inoculated in a solid MS medium supplemented with factorial combinations of 2,4-D, BA, NAA and GA3. Callus formation was evaluated weekly until the 49th day. Subsequently, new explants were inoculated at the hormonal combination that resulted in the highest callus cell proliferation and, every seven days during a period of 70 days, samples were dried and weighed to determine the callus growth pattern. NAA and GA3 were not effective for callus induction. Combinations of 2,4-D and BA resulted in callus induction and proliferation. The highest percentage of callus induction was observed with the combination of 4.52 µM 2,4-D and 4.44 µM BA. The calluses thereby produced were friable and whitish. The callus growth pattern followed a sigmoid shape. The deceleration phase started on the 56th day of culture.
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spelling Induction and growth pattern of callus from Piper permucronatum leavesPiperaceaegrowth regulatorscallogenesisABSTRACT Piper permucronatum is a perennial shrub, a medicinal plant native to the Amazon Rainforest. Traditionally, the tea of its leaves is used to combat menstrual and intestinal cramps, stomach pain, digestive problems, diarrhea, hemorrhage, and nausea. Its leaf’s essential oil is effective against Aedes aegypti larvae; its flavones and flavanones have a fungicidal effect against Clamidosporium cladosporioides and C. sphaerospermum; its hexanic extract is effective against Leishmania amazonensis. The objective of this study was to provide a protocol for callus induction from P. permucronatum leaves and an identification of the callus growth pattern, focusing on the deceleration phase, when the callus cells must be subcultured into liquid medium in order to produce a cell suspension cultures. Leaf explants were inoculated in a solid MS medium supplemented with factorial combinations of 2,4-D, BA, NAA and GA3. Callus formation was evaluated weekly until the 49th day. Subsequently, new explants were inoculated at the hormonal combination that resulted in the highest callus cell proliferation and, every seven days during a period of 70 days, samples were dried and weighed to determine the callus growth pattern. NAA and GA3 were not effective for callus induction. Combinations of 2,4-D and BA resulted in callus induction and proliferation. The highest percentage of callus induction was observed with the combination of 4.52 µM 2,4-D and 4.44 µM BA. The calluses thereby produced were friable and whitish. The callus growth pattern followed a sigmoid shape. The deceleration phase started on the 56th day of culture.Sociedade Brasileira de Plantas Medicinais2016-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-05722016000100142Revista Brasileira de Plantas Medicinais v.18 n.1 2016reponame:Revista brasileira de plantas medicinais (Online)instname:Universidade Estadual Paulista (UNESP)instacron:SBPM10.1590/1983-084X/15_098info:eu-repo/semantics/openAccessSANTOS,M.R.A.GUIMARÃES,M.C.M.PAZ,E.S.MAGALHÃES,G.M.O.SOUZA,C.A.SMOZINSKI,C.V.NOGUEIRA,W.O.eng2016-05-03T00:00:00Zoai:scielo:S1516-05722016000100142Revistahttp://www.scielo.br/scielo.php?script=sci_serial&pid=1516-0572&lng=en&nrm=isoPUBhttps://old.scielo.br/oai/scielo-oai.php||rbpm.sbpm@gmail.com1983-084X1516-0572opendoar:2016-05-03T00:00Revista brasileira de plantas medicinais (Online) - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Induction and growth pattern of callus from Piper permucronatum leaves
title Induction and growth pattern of callus from Piper permucronatum leaves
spellingShingle Induction and growth pattern of callus from Piper permucronatum leaves
SANTOS,M.R.A.
Piperaceae
growth regulators
callogenesis
title_short Induction and growth pattern of callus from Piper permucronatum leaves
title_full Induction and growth pattern of callus from Piper permucronatum leaves
title_fullStr Induction and growth pattern of callus from Piper permucronatum leaves
title_full_unstemmed Induction and growth pattern of callus from Piper permucronatum leaves
title_sort Induction and growth pattern of callus from Piper permucronatum leaves
author SANTOS,M.R.A.
author_facet SANTOS,M.R.A.
GUIMARÃES,M.C.M.
PAZ,E.S.
MAGALHÃES,G.M.O.
SOUZA,C.A.
SMOZINSKI,C.V.
NOGUEIRA,W.O.
author_role author
author2 GUIMARÃES,M.C.M.
PAZ,E.S.
MAGALHÃES,G.M.O.
SOUZA,C.A.
SMOZINSKI,C.V.
NOGUEIRA,W.O.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv SANTOS,M.R.A.
GUIMARÃES,M.C.M.
PAZ,E.S.
MAGALHÃES,G.M.O.
SOUZA,C.A.
SMOZINSKI,C.V.
NOGUEIRA,W.O.
dc.subject.por.fl_str_mv Piperaceae
growth regulators
callogenesis
topic Piperaceae
growth regulators
callogenesis
description ABSTRACT Piper permucronatum is a perennial shrub, a medicinal plant native to the Amazon Rainforest. Traditionally, the tea of its leaves is used to combat menstrual and intestinal cramps, stomach pain, digestive problems, diarrhea, hemorrhage, and nausea. Its leaf’s essential oil is effective against Aedes aegypti larvae; its flavones and flavanones have a fungicidal effect against Clamidosporium cladosporioides and C. sphaerospermum; its hexanic extract is effective against Leishmania amazonensis. The objective of this study was to provide a protocol for callus induction from P. permucronatum leaves and an identification of the callus growth pattern, focusing on the deceleration phase, when the callus cells must be subcultured into liquid medium in order to produce a cell suspension cultures. Leaf explants were inoculated in a solid MS medium supplemented with factorial combinations of 2,4-D, BA, NAA and GA3. Callus formation was evaluated weekly until the 49th day. Subsequently, new explants were inoculated at the hormonal combination that resulted in the highest callus cell proliferation and, every seven days during a period of 70 days, samples were dried and weighed to determine the callus growth pattern. NAA and GA3 were not effective for callus induction. Combinations of 2,4-D and BA resulted in callus induction and proliferation. The highest percentage of callus induction was observed with the combination of 4.52 µM 2,4-D and 4.44 µM BA. The calluses thereby produced were friable and whitish. The callus growth pattern followed a sigmoid shape. The deceleration phase started on the 56th day of culture.
publishDate 2016
dc.date.none.fl_str_mv 2016-03-01
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dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv 10.1590/1983-084X/15_098
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Plantas Medicinais
publisher.none.fl_str_mv Sociedade Brasileira de Plantas Medicinais
dc.source.none.fl_str_mv Revista Brasileira de Plantas Medicinais v.18 n.1 2016
reponame:Revista brasileira de plantas medicinais (Online)
instname:Universidade Estadual Paulista (UNESP)
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collection Revista brasileira de plantas medicinais (Online)
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