Induction and growth pattern of callus from Piper permucronatum leaves
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista brasileira de plantas medicinais (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-05722016000100142 |
Resumo: | ABSTRACT Piper permucronatum is a perennial shrub, a medicinal plant native to the Amazon Rainforest. Traditionally, the tea of its leaves is used to combat menstrual and intestinal cramps, stomach pain, digestive problems, diarrhea, hemorrhage, and nausea. Its leaf’s essential oil is effective against Aedes aegypti larvae; its flavones and flavanones have a fungicidal effect against Clamidosporium cladosporioides and C. sphaerospermum; its hexanic extract is effective against Leishmania amazonensis. The objective of this study was to provide a protocol for callus induction from P. permucronatum leaves and an identification of the callus growth pattern, focusing on the deceleration phase, when the callus cells must be subcultured into liquid medium in order to produce a cell suspension cultures. Leaf explants were inoculated in a solid MS medium supplemented with factorial combinations of 2,4-D, BA, NAA and GA3. Callus formation was evaluated weekly until the 49th day. Subsequently, new explants were inoculated at the hormonal combination that resulted in the highest callus cell proliferation and, every seven days during a period of 70 days, samples were dried and weighed to determine the callus growth pattern. NAA and GA3 were not effective for callus induction. Combinations of 2,4-D and BA resulted in callus induction and proliferation. The highest percentage of callus induction was observed with the combination of 4.52 µM 2,4-D and 4.44 µM BA. The calluses thereby produced were friable and whitish. The callus growth pattern followed a sigmoid shape. The deceleration phase started on the 56th day of culture. |
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Induction and growth pattern of callus from Piper permucronatum leavesPiperaceaegrowth regulatorscallogenesisABSTRACT Piper permucronatum is a perennial shrub, a medicinal plant native to the Amazon Rainforest. Traditionally, the tea of its leaves is used to combat menstrual and intestinal cramps, stomach pain, digestive problems, diarrhea, hemorrhage, and nausea. Its leaf’s essential oil is effective against Aedes aegypti larvae; its flavones and flavanones have a fungicidal effect against Clamidosporium cladosporioides and C. sphaerospermum; its hexanic extract is effective against Leishmania amazonensis. The objective of this study was to provide a protocol for callus induction from P. permucronatum leaves and an identification of the callus growth pattern, focusing on the deceleration phase, when the callus cells must be subcultured into liquid medium in order to produce a cell suspension cultures. Leaf explants were inoculated in a solid MS medium supplemented with factorial combinations of 2,4-D, BA, NAA and GA3. Callus formation was evaluated weekly until the 49th day. Subsequently, new explants were inoculated at the hormonal combination that resulted in the highest callus cell proliferation and, every seven days during a period of 70 days, samples were dried and weighed to determine the callus growth pattern. NAA and GA3 were not effective for callus induction. Combinations of 2,4-D and BA resulted in callus induction and proliferation. The highest percentage of callus induction was observed with the combination of 4.52 µM 2,4-D and 4.44 µM BA. The calluses thereby produced were friable and whitish. The callus growth pattern followed a sigmoid shape. The deceleration phase started on the 56th day of culture.Sociedade Brasileira de Plantas Medicinais2016-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-05722016000100142Revista Brasileira de Plantas Medicinais v.18 n.1 2016reponame:Revista brasileira de plantas medicinais (Online)instname:Universidade Estadual Paulista (UNESP)instacron:SBPM10.1590/1983-084X/15_098info:eu-repo/semantics/openAccessSANTOS,M.R.A.GUIMARÃES,M.C.M.PAZ,E.S.MAGALHÃES,G.M.O.SOUZA,C.A.SMOZINSKI,C.V.NOGUEIRA,W.O.eng2016-05-03T00:00:00Zoai:scielo:S1516-05722016000100142Revistahttp://www.scielo.br/scielo.php?script=sci_serial&pid=1516-0572&lng=en&nrm=isoPUBhttps://old.scielo.br/oai/scielo-oai.php||rbpm.sbpm@gmail.com1983-084X1516-0572opendoar:2016-05-03T00:00Revista brasileira de plantas medicinais (Online) - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Induction and growth pattern of callus from Piper permucronatum leaves |
title |
Induction and growth pattern of callus from Piper permucronatum leaves |
spellingShingle |
Induction and growth pattern of callus from Piper permucronatum leaves SANTOS,M.R.A. Piperaceae growth regulators callogenesis |
title_short |
Induction and growth pattern of callus from Piper permucronatum leaves |
title_full |
Induction and growth pattern of callus from Piper permucronatum leaves |
title_fullStr |
Induction and growth pattern of callus from Piper permucronatum leaves |
title_full_unstemmed |
Induction and growth pattern of callus from Piper permucronatum leaves |
title_sort |
Induction and growth pattern of callus from Piper permucronatum leaves |
author |
SANTOS,M.R.A. |
author_facet |
SANTOS,M.R.A. GUIMARÃES,M.C.M. PAZ,E.S. MAGALHÃES,G.M.O. SOUZA,C.A. SMOZINSKI,C.V. NOGUEIRA,W.O. |
author_role |
author |
author2 |
GUIMARÃES,M.C.M. PAZ,E.S. MAGALHÃES,G.M.O. SOUZA,C.A. SMOZINSKI,C.V. NOGUEIRA,W.O. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
SANTOS,M.R.A. GUIMARÃES,M.C.M. PAZ,E.S. MAGALHÃES,G.M.O. SOUZA,C.A. SMOZINSKI,C.V. NOGUEIRA,W.O. |
dc.subject.por.fl_str_mv |
Piperaceae growth regulators callogenesis |
topic |
Piperaceae growth regulators callogenesis |
description |
ABSTRACT Piper permucronatum is a perennial shrub, a medicinal plant native to the Amazon Rainforest. Traditionally, the tea of its leaves is used to combat menstrual and intestinal cramps, stomach pain, digestive problems, diarrhea, hemorrhage, and nausea. Its leaf’s essential oil is effective against Aedes aegypti larvae; its flavones and flavanones have a fungicidal effect against Clamidosporium cladosporioides and C. sphaerospermum; its hexanic extract is effective against Leishmania amazonensis. The objective of this study was to provide a protocol for callus induction from P. permucronatum leaves and an identification of the callus growth pattern, focusing on the deceleration phase, when the callus cells must be subcultured into liquid medium in order to produce a cell suspension cultures. Leaf explants were inoculated in a solid MS medium supplemented with factorial combinations of 2,4-D, BA, NAA and GA3. Callus formation was evaluated weekly until the 49th day. Subsequently, new explants were inoculated at the hormonal combination that resulted in the highest callus cell proliferation and, every seven days during a period of 70 days, samples were dried and weighed to determine the callus growth pattern. NAA and GA3 were not effective for callus induction. Combinations of 2,4-D and BA resulted in callus induction and proliferation. The highest percentage of callus induction was observed with the combination of 4.52 µM 2,4-D and 4.44 µM BA. The calluses thereby produced were friable and whitish. The callus growth pattern followed a sigmoid shape. The deceleration phase started on the 56th day of culture. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-05722016000100142 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-05722016000100142 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1983-084X/15_098 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Plantas Medicinais |
publisher.none.fl_str_mv |
Sociedade Brasileira de Plantas Medicinais |
dc.source.none.fl_str_mv |
Revista Brasileira de Plantas Medicinais v.18 n.1 2016 reponame:Revista brasileira de plantas medicinais (Online) instname:Universidade Estadual Paulista (UNESP) instacron:SBPM |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
SBPM |
institution |
SBPM |
reponame_str |
Revista brasileira de plantas medicinais (Online) |
collection |
Revista brasileira de plantas medicinais (Online) |
repository.name.fl_str_mv |
Revista brasileira de plantas medicinais (Online) - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
||rbpm.sbpm@gmail.com |
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1750318029709770752 |