Melia azedarach L. extract induced the production of phytocystatin in passion fruit leaves

Detalhes bibliográficos
Autor(a) principal: Rodrigues,Alyne Bispo
Data de Publicação: 2021
Outros Autores: Siqueira Junior,César Luis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Hoehnea
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2236-89062021000100402
Resumo: ABSTRACT Phytocystatins belong to the family of cysteine proteinases inhibitors. These proteinase inhibitors have crucial functions in plant defense against insects and pathogens attack. In the present study Melia azedarach L. extract acts as an elicitor of plant defense responses in passion fruit seedlings inducing the increase of a phytocystatin levels in of these plants. The leaf protein content of the seedlings was extracted 24 hours after treatment with plant extract and analyzed for the induction of proteinase inhibitor activity. The results demonstrated the induction of a ~ 60 kD phytocystatin (Pfcyst) that has in vitro inhibitory effects against papain. These findings suggest that M. azedarach plant extracts may act as a potential activator of induced resistance against insects and pathogens by upregulating cystatin proteins synthesis in passion fruits plants. Thus, the use of plant extracts as biodefensives could reduce the use of pesticides in economically important agricultural crops.
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spelling Melia azedarach L. extract induced the production of phytocystatin in passion fruit leavescinamomocystatinplant defenseplant extractABSTRACT Phytocystatins belong to the family of cysteine proteinases inhibitors. These proteinase inhibitors have crucial functions in plant defense against insects and pathogens attack. In the present study Melia azedarach L. extract acts as an elicitor of plant defense responses in passion fruit seedlings inducing the increase of a phytocystatin levels in of these plants. The leaf protein content of the seedlings was extracted 24 hours after treatment with plant extract and analyzed for the induction of proteinase inhibitor activity. The results demonstrated the induction of a ~ 60 kD phytocystatin (Pfcyst) that has in vitro inhibitory effects against papain. These findings suggest that M. azedarach plant extracts may act as a potential activator of induced resistance against insects and pathogens by upregulating cystatin proteins synthesis in passion fruits plants. Thus, the use of plant extracts as biodefensives could reduce the use of pesticides in economically important agricultural crops.Instituto de Pesquisas Ambientais2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2236-89062021000100402Hoehnea v.48 2021reponame:Hoehneainstname:Instituto de Botânica (IBT)instacron:IBT10.1590/2236-8906-98/2019info:eu-repo/semantics/openAccessRodrigues,Alyne BispoSiqueira Junior,César Luiseng2021-12-15T00:00:00Zoai:scielo:S2236-89062021000100402Revistahttp://www.ibot.sp.gov.br/publicacoes/hoehnea/sobre_hoehnea.phpPUBhttps://old.scielo.br/oai/scielo-oai.php||hoehneaibt@gmail.com2236-89060073-2877opendoar:2021-12-15T00:00Hoehnea - Instituto de Botânica (IBT)false
dc.title.none.fl_str_mv Melia azedarach L. extract induced the production of phytocystatin in passion fruit leaves
title Melia azedarach L. extract induced the production of phytocystatin in passion fruit leaves
spellingShingle Melia azedarach L. extract induced the production of phytocystatin in passion fruit leaves
Rodrigues,Alyne Bispo
cinamomo
cystatin
plant defense
plant extract
title_short Melia azedarach L. extract induced the production of phytocystatin in passion fruit leaves
title_full Melia azedarach L. extract induced the production of phytocystatin in passion fruit leaves
title_fullStr Melia azedarach L. extract induced the production of phytocystatin in passion fruit leaves
title_full_unstemmed Melia azedarach L. extract induced the production of phytocystatin in passion fruit leaves
title_sort Melia azedarach L. extract induced the production of phytocystatin in passion fruit leaves
author Rodrigues,Alyne Bispo
author_facet Rodrigues,Alyne Bispo
Siqueira Junior,César Luis
author_role author
author2 Siqueira Junior,César Luis
author2_role author
dc.contributor.author.fl_str_mv Rodrigues,Alyne Bispo
Siqueira Junior,César Luis
dc.subject.por.fl_str_mv cinamomo
cystatin
plant defense
plant extract
topic cinamomo
cystatin
plant defense
plant extract
description ABSTRACT Phytocystatins belong to the family of cysteine proteinases inhibitors. These proteinase inhibitors have crucial functions in plant defense against insects and pathogens attack. In the present study Melia azedarach L. extract acts as an elicitor of plant defense responses in passion fruit seedlings inducing the increase of a phytocystatin levels in of these plants. The leaf protein content of the seedlings was extracted 24 hours after treatment with plant extract and analyzed for the induction of proteinase inhibitor activity. The results demonstrated the induction of a ~ 60 kD phytocystatin (Pfcyst) that has in vitro inhibitory effects against papain. These findings suggest that M. azedarach plant extracts may act as a potential activator of induced resistance against insects and pathogens by upregulating cystatin proteins synthesis in passion fruits plants. Thus, the use of plant extracts as biodefensives could reduce the use of pesticides in economically important agricultural crops.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2236-89062021000100402
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2236-89062021000100402
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/2236-8906-98/2019
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Pesquisas Ambientais
publisher.none.fl_str_mv Instituto de Pesquisas Ambientais
dc.source.none.fl_str_mv Hoehnea v.48 2021
reponame:Hoehnea
instname:Instituto de Botânica (IBT)
instacron:IBT
instname_str Instituto de Botânica (IBT)
instacron_str IBT
institution IBT
reponame_str Hoehnea
collection Hoehnea
repository.name.fl_str_mv Hoehnea - Instituto de Botânica (IBT)
repository.mail.fl_str_mv ||hoehneaibt@gmail.com
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