Melia azedarach L. extract induced the production of phytocystatin in passion fruit leaves

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Alyne Bispo
Data de Publicação: 2021
Outros Autores: Siqueira Junior , César Luis
Tipo de documento: preprint
Idioma: por
Título da fonte: SciELO Preprints
Texto Completo: https://preprints.scielo.org/index.php/scielo/preprint/view/2363
Resumo: Phytocystatins belong to the family of cysteine proteinases inhibitors. These proteinase inhibitors have crucial functions in plant defense against insects and pathogens attack. In the present study Melia azedarach L. extract acts as an elicitor of plant defense responses in passion fruit seedlings inducing the increase of a phytocystatin levels in of these plants. The leaf protein content of the seedlings was extracted 24 hours after treatment with plant extract and analyzed for the induction of proteinase inhibitor activity. The results demonstrated the induction of a ~ 60 kD phytocystatin (Pfcyst) that has in vitro inhibitory effects against papain.  These findings suggest that M. azedarach plant extracts may act as a potential activator of induced resistance against insects and pathogens by upregulating cystatin proteins synthesis in passion fruits plants. Thus, the use of plant extracts as biodefensives could reduce the use of pesticides in economically important agricultural crops.
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spelling Melia azedarach L. extract induced the production of phytocystatin in passion fruit leavesExtrato de Melia azedarach L. induz a produção de uma fitocistatina em folhas de maracujácinamomocistatinadefesa vegetalextrato vegetalcinamomocystatinplant defenseplant extractPhytocystatins belong to the family of cysteine proteinases inhibitors. These proteinase inhibitors have crucial functions in plant defense against insects and pathogens attack. In the present study Melia azedarach L. extract acts as an elicitor of plant defense responses in passion fruit seedlings inducing the increase of a phytocystatin levels in of these plants. The leaf protein content of the seedlings was extracted 24 hours after treatment with plant extract and analyzed for the induction of proteinase inhibitor activity. The results demonstrated the induction of a ~ 60 kD phytocystatin (Pfcyst) that has in vitro inhibitory effects against papain.  These findings suggest that M. azedarach plant extracts may act as a potential activator of induced resistance against insects and pathogens by upregulating cystatin proteins synthesis in passion fruits plants. Thus, the use of plant extracts as biodefensives could reduce the use of pesticides in economically important agricultural crops.As fitocistatinas pertencem à familia de inibidores de proteinase cisteínica. Esses inibidores de proteinase têm função crucial na defesa de plantas contra o ataque de insetos e patógenos. No presente estudo, extrato de Melia azedarach L. atuam como um eliciador da resposta de defesa em plântulas de maracujá induzindo o aumento dos níveis de fitocistatinas nessas plantas. O conteúdo proteico foliar dessas plântulas foi extraído 24 horas após o tratamento com o extrato vegetal e analisado quanto a indução da atividade do inibidor de proteinase. Os resultados demonstraram a indução de uma fitocistatina (Pfcyst) de ~60 kDa que possui efeito inibitório in vitro contra a papaína. Essas descobertas sugerem que o extrato da planta M. azedarach pode atuar como um potencial ativador da resistência induzida contra insetos e patógenos por regular positivamente a síntese de proteínas cistatinas em plantas de maracuja. Dessa forma, o uso de extratos vegetais como biodefensivos poderia reduzir o uso de agrotóxicos em culturas agrícolas economicamente importantes.SciELO PreprintsSciELO PreprintsSciELO Preprints2021-05-25info:eu-repo/semantics/preprintinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://preprints.scielo.org/index.php/scielo/preprint/view/236310.1590/2236-8906-98/2019porhttps://preprints.scielo.org/index.php/scielo/article/view/2363/4015Copyright (c) 2021 Alyne Bispo Rodrigues, César Luis Siqueira Junior https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessRodrigues, Alyne Bispo Siqueira Junior , César Luis reponame:SciELO Preprintsinstname:SciELOinstacron:SCI2021-05-24T23:31:19Zoai:ops.preprints.scielo.org:preprint/2363Servidor de preprintshttps://preprints.scielo.org/index.php/scieloONGhttps://preprints.scielo.org/index.php/scielo/oaiscielo.submission@scielo.orgopendoar:2021-05-24T23:31:19SciELO Preprints - SciELOfalse
dc.title.none.fl_str_mv Melia azedarach L. extract induced the production of phytocystatin in passion fruit leaves
Extrato de Melia azedarach L. induz a produção de uma fitocistatina em folhas de maracujá
title Melia azedarach L. extract induced the production of phytocystatin in passion fruit leaves
spellingShingle Melia azedarach L. extract induced the production of phytocystatin in passion fruit leaves
Rodrigues, Alyne Bispo
cinamomo
cistatina
defesa vegetal
extrato vegetal
cinamomo
cystatin
plant defense
plant extract
title_short Melia azedarach L. extract induced the production of phytocystatin in passion fruit leaves
title_full Melia azedarach L. extract induced the production of phytocystatin in passion fruit leaves
title_fullStr Melia azedarach L. extract induced the production of phytocystatin in passion fruit leaves
title_full_unstemmed Melia azedarach L. extract induced the production of phytocystatin in passion fruit leaves
title_sort Melia azedarach L. extract induced the production of phytocystatin in passion fruit leaves
author Rodrigues, Alyne Bispo
author_facet Rodrigues, Alyne Bispo
Siqueira Junior , César Luis
author_role author
author2 Siqueira Junior , César Luis
author2_role author
dc.contributor.author.fl_str_mv Rodrigues, Alyne Bispo
Siqueira Junior , César Luis
dc.subject.por.fl_str_mv cinamomo
cistatina
defesa vegetal
extrato vegetal
cinamomo
cystatin
plant defense
plant extract
topic cinamomo
cistatina
defesa vegetal
extrato vegetal
cinamomo
cystatin
plant defense
plant extract
description Phytocystatins belong to the family of cysteine proteinases inhibitors. These proteinase inhibitors have crucial functions in plant defense against insects and pathogens attack. In the present study Melia azedarach L. extract acts as an elicitor of plant defense responses in passion fruit seedlings inducing the increase of a phytocystatin levels in of these plants. The leaf protein content of the seedlings was extracted 24 hours after treatment with plant extract and analyzed for the induction of proteinase inhibitor activity. The results demonstrated the induction of a ~ 60 kD phytocystatin (Pfcyst) that has in vitro inhibitory effects against papain.  These findings suggest that M. azedarach plant extracts may act as a potential activator of induced resistance against insects and pathogens by upregulating cystatin proteins synthesis in passion fruits plants. Thus, the use of plant extracts as biodefensives could reduce the use of pesticides in economically important agricultural crops.
publishDate 2021
dc.date.none.fl_str_mv 2021-05-25
dc.type.driver.fl_str_mv info:eu-repo/semantics/preprint
info:eu-repo/semantics/publishedVersion
format preprint
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://preprints.scielo.org/index.php/scielo/preprint/view/2363
10.1590/2236-8906-98/2019
url https://preprints.scielo.org/index.php/scielo/preprint/view/2363
identifier_str_mv 10.1590/2236-8906-98/2019
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://preprints.scielo.org/index.php/scielo/article/view/2363/4015
dc.rights.driver.fl_str_mv Copyright (c) 2021 Alyne Bispo Rodrigues, César Luis Siqueira Junior
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2021 Alyne Bispo Rodrigues, César Luis Siqueira Junior
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv SciELO Preprints
SciELO Preprints
SciELO Preprints
publisher.none.fl_str_mv SciELO Preprints
SciELO Preprints
SciELO Preprints
dc.source.none.fl_str_mv reponame:SciELO Preprints
instname:SciELO
instacron:SCI
instname_str SciELO
instacron_str SCI
institution SCI
reponame_str SciELO Preprints
collection SciELO Preprints
repository.name.fl_str_mv SciELO Preprints - SciELO
repository.mail.fl_str_mv scielo.submission@scielo.org
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