Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species

Detalhes bibliográficos
Autor(a) principal: Silva, Bruno José Martins da
Data de Publicação: 2016
Outros Autores: Silva, Raquel Raick P. da, Farias, Ana Paula Drummond Rodrigues de, Farias, Luis Henrique Seabra, Nascimento, José Luiz Martins do, Silva, Edilene Oliveira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/3816
Resumo: Leishmaniasis are a neglected group of emerging diseases that have been found in 98 countries and are caused by protozoa of the genus Leishmania. The therapy for leishmaniasis causes several side effects and leads to drug-resistant strains. Natural products from plants have exhibited activities against Leishmania in various experimental models. Physalis angulata is a widely used plant in popular medicine, and in the literature it has well-documented leishmanicidal activity. However, its mechanism of action is still unknown. Thus, this study aims to evaluate the mechanism driving the leishmanicidal activity of an aqueous extract of P. angulata root (AEPa). AEPa was effective against both promastigotes and intracellular amastigote forms of Leishmania amazonensis. This effect was mediated by an increase of reactive oxygen species (ROS), but not of nitric oxide (NO). The increased production of ROS induces cell death by phenotypes seems by apoptosis cell death in Leishmania, but not autophagy or necrosis. In addition, morphological analysis of macrophages showed that AEPa induced a high number of cytoplasmic projections, increased the volume of cytoplasm and number of vacuoles, caused cytoskeleton alterations and resulted in high spreading ability. AEPa also promoted superoxide anion (O2(-)) production in both uninfected macrophages and those infected with Leishmania. Therefore, these results revealed that AEPa causes cell death by phenotypes seems by apoptosis cell death in L. amazonensis and modulates macrophage activation through morphofunctional alterations and O2(-) generation to induce Leishmania death.
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spelling Silva, Bruno José Martins daSilva, Raquel Raick P. daFarias, Ana Paula Drummond Rodrigues deFarias, Luis Henrique SeabraNascimento, José Luiz Martins doSilva, Edilene Oliveira2019-07-08T14:04:06Z2019-07-08T14:04:06Z2016SILVA, Bruno José Martins da et al. Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species. Micron, v. 82, n. x, p. 25-32, Mar. 2016.0968-4328https://patua.iec.gov.br/handle/iec/381610.1016/j.micron.2015.12.001Leishmaniasis are a neglected group of emerging diseases that have been found in 98 countries and are caused by protozoa of the genus Leishmania. The therapy for leishmaniasis causes several side effects and leads to drug-resistant strains. Natural products from plants have exhibited activities against Leishmania in various experimental models. Physalis angulata is a widely used plant in popular medicine, and in the literature it has well-documented leishmanicidal activity. However, its mechanism of action is still unknown. Thus, this study aims to evaluate the mechanism driving the leishmanicidal activity of an aqueous extract of P. angulata root (AEPa). AEPa was effective against both promastigotes and intracellular amastigote forms of Leishmania amazonensis. This effect was mediated by an increase of reactive oxygen species (ROS), but not of nitric oxide (NO). The increased production of ROS induces cell death by phenotypes seems by apoptosis cell death in Leishmania, but not autophagy or necrosis. In addition, morphological analysis of macrophages showed that AEPa induced a high number of cytoplasmic projections, increased the volume of cytoplasm and number of vacuoles, caused cytoskeleton alterations and resulted in high spreading ability. AEPa also promoted superoxide anion (O2(-)) production in both uninfected macrophages and those infected with Leishmania. Therefore, these results revealed that AEPa causes cell death by phenotypes seems by apoptosis cell death in L. amazonensis and modulates macrophage activation through morphofunctional alterations and O2(-) generation to induce Leishmania death.Federal University of Pará. Institute of Biological Sciences. Laboratory of Parasitology and Laboratory of Structural Biology. Belém, PA, Brazil / National Institute of Science and Technology in Structural Biology and Bioimaging. Rio de Janeiro, RJ, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Parasitology and Laboratory of Structural Biology. Belém, PA, Brazil / National Institute of Science and Technology in Structural Biology and Bioimaging. Rio de Janeiro, RJ, Brazil.National Institute of Science and Technology in Structural Biology and Bioimaging. Rio de Janeiro, RJ, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Microcopia Eletrônica. Ananindeua, PA, Brasil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Parasitology and Laboratory of Structural Biology. Belém, PA, Brazil / National Institute of Science and Technology in Structural Biology and Bioimaging. Rio de Janeiro, RJ, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Neurochemistry Molecular and Cellular. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Parasitology and Laboratory of Structural Biology. Belém, PA, Brazil / National Institute of Science and Technology in Structural Biology and Bioimaging. Rio de Janeiro, RJ, Brazil.engElsevierPhysalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen speciesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePhysalis / químicaLeishmaniose / parasitologiaLeishmaniose / tratamento farmacológicoPlantas Medicinais / efeitos de drogasAtivação de Macrófagos / efeitos dos fármacosEspécies Reativas de Oxigênioinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALPhysalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species.pdfPhysalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species.pdfapplication/pdf551083https://patua.iec.gov.br/bitstreams/3e6ad10d-3c56-4653-9a37-08be3f56768c/downloadc9a9c128e29cac82a5d7fdf3f4e6da73MD51LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species
title Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species
spellingShingle Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species
Silva, Bruno José Martins da
Physalis / química
Leishmaniose / parasitologia
Leishmaniose / tratamento farmacológico
Plantas Medicinais / efeitos de drogas
Ativação de Macrófagos / efeitos dos fármacos
Espécies Reativas de Oxigênio
title_short Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species
title_full Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species
title_fullStr Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species
title_full_unstemmed Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species
title_sort Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species
author Silva, Bruno José Martins da
author_facet Silva, Bruno José Martins da
Silva, Raquel Raick P. da
Farias, Ana Paula Drummond Rodrigues de
Farias, Luis Henrique Seabra
Nascimento, José Luiz Martins do
Silva, Edilene Oliveira
author_role author
author2 Silva, Raquel Raick P. da
Farias, Ana Paula Drummond Rodrigues de
Farias, Luis Henrique Seabra
Nascimento, José Luiz Martins do
Silva, Edilene Oliveira
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Bruno José Martins da
Silva, Raquel Raick P. da
Farias, Ana Paula Drummond Rodrigues de
Farias, Luis Henrique Seabra
Nascimento, José Luiz Martins do
Silva, Edilene Oliveira
dc.subject.decsPrimary.pt_BR.fl_str_mv Physalis / química
Leishmaniose / parasitologia
Leishmaniose / tratamento farmacológico
Plantas Medicinais / efeitos de drogas
Ativação de Macrófagos / efeitos dos fármacos
Espécies Reativas de Oxigênio
topic Physalis / química
Leishmaniose / parasitologia
Leishmaniose / tratamento farmacológico
Plantas Medicinais / efeitos de drogas
Ativação de Macrófagos / efeitos dos fármacos
Espécies Reativas de Oxigênio
description Leishmaniasis are a neglected group of emerging diseases that have been found in 98 countries and are caused by protozoa of the genus Leishmania. The therapy for leishmaniasis causes several side effects and leads to drug-resistant strains. Natural products from plants have exhibited activities against Leishmania in various experimental models. Physalis angulata is a widely used plant in popular medicine, and in the literature it has well-documented leishmanicidal activity. However, its mechanism of action is still unknown. Thus, this study aims to evaluate the mechanism driving the leishmanicidal activity of an aqueous extract of P. angulata root (AEPa). AEPa was effective against both promastigotes and intracellular amastigote forms of Leishmania amazonensis. This effect was mediated by an increase of reactive oxygen species (ROS), but not of nitric oxide (NO). The increased production of ROS induces cell death by phenotypes seems by apoptosis cell death in Leishmania, but not autophagy or necrosis. In addition, morphological analysis of macrophages showed that AEPa induced a high number of cytoplasmic projections, increased the volume of cytoplasm and number of vacuoles, caused cytoskeleton alterations and resulted in high spreading ability. AEPa also promoted superoxide anion (O2(-)) production in both uninfected macrophages and those infected with Leishmania. Therefore, these results revealed that AEPa causes cell death by phenotypes seems by apoptosis cell death in L. amazonensis and modulates macrophage activation through morphofunctional alterations and O2(-) generation to induce Leishmania death.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2019-07-08T14:04:06Z
dc.date.available.fl_str_mv 2019-07-08T14:04:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, Bruno José Martins da et al. Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species. Micron, v. 82, n. x, p. 25-32, Mar. 2016.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/3816
dc.identifier.issn.-.fl_str_mv 0968-4328
dc.identifier.doi.-.fl_str_mv 10.1016/j.micron.2015.12.001
identifier_str_mv SILVA, Bruno José Martins da et al. Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species. Micron, v. 82, n. x, p. 25-32, Mar. 2016.
0968-4328
10.1016/j.micron.2015.12.001
url https://patua.iec.gov.br/handle/iec/3816
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