Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/3816 |
Resumo: | Leishmaniasis are a neglected group of emerging diseases that have been found in 98 countries and are caused by protozoa of the genus Leishmania. The therapy for leishmaniasis causes several side effects and leads to drug-resistant strains. Natural products from plants have exhibited activities against Leishmania in various experimental models. Physalis angulata is a widely used plant in popular medicine, and in the literature it has well-documented leishmanicidal activity. However, its mechanism of action is still unknown. Thus, this study aims to evaluate the mechanism driving the leishmanicidal activity of an aqueous extract of P. angulata root (AEPa). AEPa was effective against both promastigotes and intracellular amastigote forms of Leishmania amazonensis. This effect was mediated by an increase of reactive oxygen species (ROS), but not of nitric oxide (NO). The increased production of ROS induces cell death by phenotypes seems by apoptosis cell death in Leishmania, but not autophagy or necrosis. In addition, morphological analysis of macrophages showed that AEPa induced a high number of cytoplasmic projections, increased the volume of cytoplasm and number of vacuoles, caused cytoskeleton alterations and resulted in high spreading ability. AEPa also promoted superoxide anion (O2(-)) production in both uninfected macrophages and those infected with Leishmania. Therefore, these results revealed that AEPa causes cell death by phenotypes seems by apoptosis cell death in L. amazonensis and modulates macrophage activation through morphofunctional alterations and O2(-) generation to induce Leishmania death. |
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Silva, Bruno José Martins daSilva, Raquel Raick P. daFarias, Ana Paula Drummond Rodrigues deFarias, Luis Henrique SeabraNascimento, José Luiz Martins doSilva, Edilene Oliveira2019-07-08T14:04:06Z2019-07-08T14:04:06Z2016SILVA, Bruno José Martins da et al. Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species. Micron, v. 82, n. x, p. 25-32, Mar. 2016.0968-4328https://patua.iec.gov.br/handle/iec/381610.1016/j.micron.2015.12.001Leishmaniasis are a neglected group of emerging diseases that have been found in 98 countries and are caused by protozoa of the genus Leishmania. The therapy for leishmaniasis causes several side effects and leads to drug-resistant strains. Natural products from plants have exhibited activities against Leishmania in various experimental models. Physalis angulata is a widely used plant in popular medicine, and in the literature it has well-documented leishmanicidal activity. However, its mechanism of action is still unknown. Thus, this study aims to evaluate the mechanism driving the leishmanicidal activity of an aqueous extract of P. angulata root (AEPa). AEPa was effective against both promastigotes and intracellular amastigote forms of Leishmania amazonensis. This effect was mediated by an increase of reactive oxygen species (ROS), but not of nitric oxide (NO). The increased production of ROS induces cell death by phenotypes seems by apoptosis cell death in Leishmania, but not autophagy or necrosis. In addition, morphological analysis of macrophages showed that AEPa induced a high number of cytoplasmic projections, increased the volume of cytoplasm and number of vacuoles, caused cytoskeleton alterations and resulted in high spreading ability. AEPa also promoted superoxide anion (O2(-)) production in both uninfected macrophages and those infected with Leishmania. Therefore, these results revealed that AEPa causes cell death by phenotypes seems by apoptosis cell death in L. amazonensis and modulates macrophage activation through morphofunctional alterations and O2(-) generation to induce Leishmania death.Federal University of Pará. Institute of Biological Sciences. Laboratory of Parasitology and Laboratory of Structural Biology. Belém, PA, Brazil / National Institute of Science and Technology in Structural Biology and Bioimaging. Rio de Janeiro, RJ, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Parasitology and Laboratory of Structural Biology. Belém, PA, Brazil / National Institute of Science and Technology in Structural Biology and Bioimaging. Rio de Janeiro, RJ, Brazil.National Institute of Science and Technology in Structural Biology and Bioimaging. Rio de Janeiro, RJ, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Microcopia Eletrônica. Ananindeua, PA, Brasil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Parasitology and Laboratory of Structural Biology. Belém, PA, Brazil / National Institute of Science and Technology in Structural Biology and Bioimaging. Rio de Janeiro, RJ, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Neurochemistry Molecular and Cellular. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Parasitology and Laboratory of Structural Biology. Belém, PA, Brazil / National Institute of Science and Technology in Structural Biology and Bioimaging. Rio de Janeiro, RJ, Brazil.engElsevierPhysalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen speciesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePhysalis / químicaLeishmaniose / parasitologiaLeishmaniose / tratamento farmacológicoPlantas Medicinais / efeitos de drogasAtivação de Macrófagos / efeitos dos fármacosEspécies Reativas de Oxigênioinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALPhysalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species.pdfPhysalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species.pdfapplication/pdf551083https://patua.iec.gov.br/bitstreams/3e6ad10d-3c56-4653-9a37-08be3f56768c/downloadc9a9c128e29cac82a5d7fdf3f4e6da73MD51LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv |
Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species |
title |
Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species |
spellingShingle |
Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species Silva, Bruno José Martins da Physalis / química Leishmaniose / parasitologia Leishmaniose / tratamento farmacológico Plantas Medicinais / efeitos de drogas Ativação de Macrófagos / efeitos dos fármacos Espécies Reativas de Oxigênio |
title_short |
Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species |
title_full |
Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species |
title_fullStr |
Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species |
title_full_unstemmed |
Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species |
title_sort |
Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species |
author |
Silva, Bruno José Martins da |
author_facet |
Silva, Bruno José Martins da Silva, Raquel Raick P. da Farias, Ana Paula Drummond Rodrigues de Farias, Luis Henrique Seabra Nascimento, José Luiz Martins do Silva, Edilene Oliveira |
author_role |
author |
author2 |
Silva, Raquel Raick P. da Farias, Ana Paula Drummond Rodrigues de Farias, Luis Henrique Seabra Nascimento, José Luiz Martins do Silva, Edilene Oliveira |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Bruno José Martins da Silva, Raquel Raick P. da Farias, Ana Paula Drummond Rodrigues de Farias, Luis Henrique Seabra Nascimento, José Luiz Martins do Silva, Edilene Oliveira |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Physalis / química Leishmaniose / parasitologia Leishmaniose / tratamento farmacológico Plantas Medicinais / efeitos de drogas Ativação de Macrófagos / efeitos dos fármacos Espécies Reativas de Oxigênio |
topic |
Physalis / química Leishmaniose / parasitologia Leishmaniose / tratamento farmacológico Plantas Medicinais / efeitos de drogas Ativação de Macrófagos / efeitos dos fármacos Espécies Reativas de Oxigênio |
description |
Leishmaniasis are a neglected group of emerging diseases that have been found in 98 countries and are caused by protozoa of the genus Leishmania. The therapy for leishmaniasis causes several side effects and leads to drug-resistant strains. Natural products from plants have exhibited activities against Leishmania in various experimental models. Physalis angulata is a widely used plant in popular medicine, and in the literature it has well-documented leishmanicidal activity. However, its mechanism of action is still unknown. Thus, this study aims to evaluate the mechanism driving the leishmanicidal activity of an aqueous extract of P. angulata root (AEPa). AEPa was effective against both promastigotes and intracellular amastigote forms of Leishmania amazonensis. This effect was mediated by an increase of reactive oxygen species (ROS), but not of nitric oxide (NO). The increased production of ROS induces cell death by phenotypes seems by apoptosis cell death in Leishmania, but not autophagy or necrosis. In addition, morphological analysis of macrophages showed that AEPa induced a high number of cytoplasmic projections, increased the volume of cytoplasm and number of vacuoles, caused cytoskeleton alterations and resulted in high spreading ability. AEPa also promoted superoxide anion (O2(-)) production in both uninfected macrophages and those infected with Leishmania. Therefore, these results revealed that AEPa causes cell death by phenotypes seems by apoptosis cell death in L. amazonensis and modulates macrophage activation through morphofunctional alterations and O2(-) generation to induce Leishmania death. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2019-07-08T14:04:06Z |
dc.date.available.fl_str_mv |
2019-07-08T14:04:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, Bruno José Martins da et al. Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species. Micron, v. 82, n. x, p. 25-32, Mar. 2016. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/3816 |
dc.identifier.issn.-.fl_str_mv |
0968-4328 |
dc.identifier.doi.-.fl_str_mv |
10.1016/j.micron.2015.12.001 |
identifier_str_mv |
SILVA, Bruno José Martins da et al. Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species. Micron, v. 82, n. x, p. 25-32, Mar. 2016. 0968-4328 10.1016/j.micron.2015.12.001 |
url |
https://patua.iec.gov.br/handle/iec/3816 |
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eng |
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eng |
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Elsevier |
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Elsevier |
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