Effectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Marques, Fernanda A
Data de Publicação: 2017
Outros Autores: Soares, Rodrigo P, Almeida, Gregório G, Souza, Carolina Carvalho de, Melo, Maria Norma, Pinto, Sebastião Alves, Quixabeira, Valéria Bernadete Leite, Pereira, Ledice I, Dorta, Miriam L, Dias, Fatima Ribeiro, Silveira, Fernando Tobias, Silva, Sydnei Magno da, Gontijo, Célia Maria Ferreira, Tafuri, Wagner L
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/2528
Resumo: American tegumentary leishmaniasis (ATL) is a neglected disease widely distributed in Latin America. In Brazil, it is caused by different Leishmania species belonging to the Subgenera Viannia and Leishmania. ATL diagnosis is routinely based on clinical, epidemiological, parasitological and immunological (delayed-type hypersensitivity skin test-DTH) evidences. The main objective of this work was to determine the efficacy of a previous immunohistochemical (IHC) method developed by our group. Seventy eight skin biopsies from patients with different ATL clinical forms and origins were evaluated. The method was previously standardized in ATL patients from the municipality of Caratinga, Minas Gerais, Brazil, all infected with Leishmania (V.) braziliensis. Here, it is evaluated in patients from the North, Southeast and Midwest regions of Brazil. Clinical, parasitological (biopsy PCR) and immunological (Montenegro skin test-MST) diagnosis were performed prior to IHC procedure. The IHC procedure detected 70.5% of the cases having a high agreement with MST diagnosis (kappa = 0.84). A distinguished contribution of this work is that IHC succeed in diagnosing some negative DTH patients. Those were infected with Leishmania (L.) amazonensis, commonly causing the anergic form of the disease. In conclusion, IHC succeed in detecting ATL caused by different Leishmania species from various geographic regions and clinical status. Although it was not able to detect ATL in all patients, it was better than MST providing an additional tool for the diagnosis of ATL patients. There was no significant correlation between clinical forms and histological features including the presence of necrosis.
id IEC-2_0c4e9614f8598cfc6c82c03df059db0a
oai_identifier_str oai:patua.iec.gov.br:iec/2528
network_acronym_str IEC-2
network_name_str Repositório Digital do Instituto Evandro Chagas (Patuá)
repository_id_str
spelling Marques, Fernanda ASoares, Rodrigo PAlmeida, Gregório GSouza, Carolina Carvalho deMelo, Maria NormaPinto, Sebastião AlvesQuixabeira, Valéria Bernadete LeitePereira, Ledice IDorta, Miriam LDias, Fatima RibeiroSilveira, Fernando TobiasSilva, Sydnei Magno daGontijo, Célia Maria FerreiraTafuri, Wagner L2017-02-15T18:39:13Z2017-02-15T18:39:13Z2017MARQUES, Fernanda A. et al. Effectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis. Parasitology International, v. 66, p. 884–888, 2017. DOI: https://doi.org/10.1016/j.parint.2016.10.003.1873-0329https://patua.iec.gov.br/handle/iec/252810.1016/j.parint.2016.10.003American tegumentary leishmaniasis (ATL) is a neglected disease widely distributed in Latin America. In Brazil, it is caused by different Leishmania species belonging to the Subgenera Viannia and Leishmania. ATL diagnosis is routinely based on clinical, epidemiological, parasitological and immunological (delayed-type hypersensitivity skin test-DTH) evidences. The main objective of this work was to determine the efficacy of a previous immunohistochemical (IHC) method developed by our group. Seventy eight skin biopsies from patients with different ATL clinical forms and origins were evaluated. The method was previously standardized in ATL patients from the municipality of Caratinga, Minas Gerais, Brazil, all infected with Leishmania (V.) braziliensis. Here, it is evaluated in patients from the North, Southeast and Midwest regions of Brazil. Clinical, parasitological (biopsy PCR) and immunological (Montenegro skin test-MST) diagnosis were performed prior to IHC procedure. The IHC procedure detected 70.5% of the cases having a high agreement with MST diagnosis (kappa = 0.84). A distinguished contribution of this work is that IHC succeed in diagnosing some negative DTH patients. Those were infected with Leishmania (L.) amazonensis, commonly causing the anergic form of the disease. In conclusion, IHC succeed in detecting ATL caused by different Leishmania species from various geographic regions and clinical status. Although it was not able to detect ATL in all patients, it was better than MST providing an additional tool for the diagnosis of ATL patients. There was no significant correlation between clinical forms and histological features including the presence of necrosis.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisas Renê Rachou. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil.Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia, GO, Brazil.Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia, GO, Brazil.Universidade Federal de Goiás. Faculdade de Medicina. Instituto Goiano de Oncologia e Hematologia. Goiânia, GO, Brazil.Universidade Federal de Goiás. Faculdade de Medicina. Instituto Goiano de Oncologia e Hematologia. Goiânia, GO, Brazil.Universidade Federal de Goiás. Faculdade de Medicina. Instituto Goiano de Oncologia e Hematologia. Goiânia, GO, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Departamento de Imunologia, Microbiologia e Parasitologia. Minas Gerais, MG, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisas Renê Rachou. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Belo Horizonte, MG, Brazil.engElsevierEffectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLeishmania / imunologiaLeishmaniose Tegumentar AmericanaLeishmania / isolamento & purificaçãoLeishmania braziliensis / imunologiaLeishmania braziliensis / isolamento & purificaçãoLeishmaniose Cutânea / diagnósticoLeishmaniose Cutânea / epidemiologiaLeishmaniose Cutânea / parasitologiaPele / parasitologiaPele / patologiaImuno-Histoquímica / métodosProtocolo Imuno-HistoquímicoBrasil / epidemiologiainfo:eu-repo/semantics/embargoedAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECTHUMBNAILEffectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis.pdf.jpgEffectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis.pdf.jpgGenerated Thumbnailimage/jpeg1649https://patua.iec.gov.br/bitstreams/4bf7870d-330c-4c6d-b1fa-bb4de5993224/downloadc29fd858e97c0a736dcb08c2d9eb4effMD55Acesso Embargado.pdf.jpgAcesso Embargado.pdf.jpgGenerated Thumbnailimage/jpeg3095https://patua.iec.gov.br/bitstreams/195077a3-7bf8-42d0-8f5f-0275a759b52b/download71859d578212107f7f8c49a4ce09d9eeMD510LICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/53bd36f7-cadb-4a23-92a3-ae589b3adda4/download52f1732ea66fbd1123abe39f5373b797MD52TEXTEffectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis.pdf.txtEffectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis.pdf.txtExtracted texttext/plain23826https://patua.iec.gov.br/bitstreams/c25f01c3-18f1-4ce4-afca-96cfa8b1dfc8/download5f82746364d1d493798624d653066baeMD53Acesso Embargado.pdf.txtAcesso Embargado.pdf.txtExtracted texttext/plain2https://patua.iec.gov.br/bitstreams/3f252f2c-d8e9-4b00-a15e-730a314f26cb/downloade1c06d85ae7b8b032bef47e42e4c08f9MD59ORIGINALAcesso Embargado.pdfAcesso Embargado.pdfapplication/pdf551083https://patua.iec.gov.br/bitstreams/049ae75f-a08e-4b54-8db3-ed2a43267c85/downloadc9a9c128e29cac82a5d7fdf3f4e6da73MD56iec/25282022-10-21 00:02:34.925oai:patua.iec.gov.br:iec/2528https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-21T00:02:34Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4=
dc.title.pt_BR.fl_str_mv Effectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis
title Effectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis
spellingShingle Effectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis
Marques, Fernanda A
Leishmania / imunologia
Leishmaniose Tegumentar Americana
Leishmania / isolamento & purificação
Leishmania braziliensis / imunologia
Leishmania braziliensis / isolamento & purificação
Leishmaniose Cutânea / diagnóstico
Leishmaniose Cutânea / epidemiologia
Leishmaniose Cutânea / parasitologia
Pele / parasitologia
Pele / patologia
Imuno-Histoquímica / métodos
Protocolo Imuno-Histoquímico
Brasil / epidemiologia
title_short Effectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis
title_full Effectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis
title_fullStr Effectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis
title_full_unstemmed Effectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis
title_sort Effectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis
author Marques, Fernanda A
author_facet Marques, Fernanda A
Soares, Rodrigo P
Almeida, Gregório G
Souza, Carolina Carvalho de
Melo, Maria Norma
Pinto, Sebastião Alves
Quixabeira, Valéria Bernadete Leite
Pereira, Ledice I
Dorta, Miriam L
Dias, Fatima Ribeiro
Silveira, Fernando Tobias
Silva, Sydnei Magno da
Gontijo, Célia Maria Ferreira
Tafuri, Wagner L
author_role author
author2 Soares, Rodrigo P
Almeida, Gregório G
Souza, Carolina Carvalho de
Melo, Maria Norma
Pinto, Sebastião Alves
Quixabeira, Valéria Bernadete Leite
Pereira, Ledice I
Dorta, Miriam L
Dias, Fatima Ribeiro
Silveira, Fernando Tobias
Silva, Sydnei Magno da
Gontijo, Célia Maria Ferreira
Tafuri, Wagner L
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Marques, Fernanda A
Soares, Rodrigo P
Almeida, Gregório G
Souza, Carolina Carvalho de
Melo, Maria Norma
Pinto, Sebastião Alves
Quixabeira, Valéria Bernadete Leite
Pereira, Ledice I
Dorta, Miriam L
Dias, Fatima Ribeiro
Silveira, Fernando Tobias
Silva, Sydnei Magno da
Gontijo, Célia Maria Ferreira
Tafuri, Wagner L
dc.subject.decsPrimary.pt_BR.fl_str_mv Leishmania / imunologia
Leishmaniose Tegumentar Americana
Leishmania / isolamento & purificação
Leishmania braziliensis / imunologia
Leishmania braziliensis / isolamento & purificação
Leishmaniose Cutânea / diagnóstico
Leishmaniose Cutânea / epidemiologia
Leishmaniose Cutânea / parasitologia
Pele / parasitologia
Pele / patologia
Imuno-Histoquímica / métodos
Protocolo Imuno-Histoquímico
Brasil / epidemiologia
topic Leishmania / imunologia
Leishmaniose Tegumentar Americana
Leishmania / isolamento & purificação
Leishmania braziliensis / imunologia
Leishmania braziliensis / isolamento & purificação
Leishmaniose Cutânea / diagnóstico
Leishmaniose Cutânea / epidemiologia
Leishmaniose Cutânea / parasitologia
Pele / parasitologia
Pele / patologia
Imuno-Histoquímica / métodos
Protocolo Imuno-Histoquímico
Brasil / epidemiologia
description American tegumentary leishmaniasis (ATL) is a neglected disease widely distributed in Latin America. In Brazil, it is caused by different Leishmania species belonging to the Subgenera Viannia and Leishmania. ATL diagnosis is routinely based on clinical, epidemiological, parasitological and immunological (delayed-type hypersensitivity skin test-DTH) evidences. The main objective of this work was to determine the efficacy of a previous immunohistochemical (IHC) method developed by our group. Seventy eight skin biopsies from patients with different ATL clinical forms and origins were evaluated. The method was previously standardized in ATL patients from the municipality of Caratinga, Minas Gerais, Brazil, all infected with Leishmania (V.) braziliensis. Here, it is evaluated in patients from the North, Southeast and Midwest regions of Brazil. Clinical, parasitological (biopsy PCR) and immunological (Montenegro skin test-MST) diagnosis were performed prior to IHC procedure. The IHC procedure detected 70.5% of the cases having a high agreement with MST diagnosis (kappa = 0.84). A distinguished contribution of this work is that IHC succeed in diagnosing some negative DTH patients. Those were infected with Leishmania (L.) amazonensis, commonly causing the anergic form of the disease. In conclusion, IHC succeed in detecting ATL caused by different Leishmania species from various geographic regions and clinical status. Although it was not able to detect ATL in all patients, it was better than MST providing an additional tool for the diagnosis of ATL patients. There was no significant correlation between clinical forms and histological features including the presence of necrosis.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-02-15T18:39:13Z
dc.date.available.fl_str_mv 2017-02-15T18:39:13Z
dc.date.issued.fl_str_mv 2017
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MARQUES, Fernanda A. et al. Effectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis. Parasitology International, v. 66, p. 884–888, 2017. DOI: https://doi.org/10.1016/j.parint.2016.10.003.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/2528
dc.identifier.issn.-.fl_str_mv 1873-0329
dc.identifier.doi.pt_BR.fl_str_mv 10.1016/j.parint.2016.10.003
identifier_str_mv MARQUES, Fernanda A. et al. Effectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis. Parasitology International, v. 66, p. 884–888, 2017. DOI: https://doi.org/10.1016/j.parint.2016.10.003.
1873-0329
10.1016/j.parint.2016.10.003
url https://patua.iec.gov.br/handle/iec/2528
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Digital do Instituto Evandro Chagas (Patuá)
instname:Instituto Evandro Chagas (IEC)
instacron:IEC
instname_str Instituto Evandro Chagas (IEC)
instacron_str IEC
institution IEC
reponame_str Repositório Digital do Instituto Evandro Chagas (Patuá)
collection Repositório Digital do Instituto Evandro Chagas (Patuá)
bitstream.url.fl_str_mv https://patua.iec.gov.br/bitstreams/4bf7870d-330c-4c6d-b1fa-bb4de5993224/download
https://patua.iec.gov.br/bitstreams/195077a3-7bf8-42d0-8f5f-0275a759b52b/download
https://patua.iec.gov.br/bitstreams/53bd36f7-cadb-4a23-92a3-ae589b3adda4/download
https://patua.iec.gov.br/bitstreams/c25f01c3-18f1-4ce4-afca-96cfa8b1dfc8/download
https://patua.iec.gov.br/bitstreams/3f252f2c-d8e9-4b00-a15e-730a314f26cb/download
https://patua.iec.gov.br/bitstreams/049ae75f-a08e-4b54-8db3-ed2a43267c85/download
bitstream.checksum.fl_str_mv c29fd858e97c0a736dcb08c2d9eb4eff
71859d578212107f7f8c49a4ce09d9ee
52f1732ea66fbd1123abe39f5373b797
5f82746364d1d493798624d653066bae
e1c06d85ae7b8b032bef47e42e4c08f9
c9a9c128e29cac82a5d7fdf3f4e6da73
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)
repository.mail.fl_str_mv clariceneta@iec.gov.br || Biblioteca@iec.gov.br
_version_ 1787533077064450048