Effectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/2844 |
Resumo: | American tegumentary leishmaniasis (ATL) is a neglected disease widely distributed in Latin America. In Brazil, it is caused by different Leishmania species belonging to the Subgenera Viannia and Leishmania. ATL diagnosis is routinely based on clinical, epidemiological, parasitological and immunological (delayed-type hypersensitivity skin test-DTH) evidences. The main objective of this work was to determine the efficacy of a previous immunohistochemical (IHC) method developed by our group. Seventy eight skin biopsies from patients with different ATL clinical forms and origins were evaluated. The method was previously standardized in ATL patients from the municipality of Caratinga, Minas Gerais, Brazil, all infected with Leishmania (V.) braziliensis. Here, it is evaluated in patients from the North, Southeast and Midwest regions of Brazil. Clinical, parasitological (biopsy PCR) and immunological (Montenegro skin test-MST) diagnosis were performed prior to IHC procedure. The IHC procedure detected 70.5% of the cases having a high agreement with MST diagnosis (kappa=0.84). A distinguished contribution of this work is that IHC succeed in diagnosing some negative DTH patients. Those were infected with Leishmania (L.) amazonensis, commonly causing the anergic form of the disease. In conclusion, IHC succeed in detecting ATL caused by different Leishmania species from various geographic regions and clinical status. Although it was not able to detect ATL in all patients, it was better than MST providing an additional tool for the diagnosis of ATL patients. There was no significant correlation between clinical forms and histological features including the presence of necrosis |
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Marques, Fernanda ASoares, Rodrigo PAlmeida, Gregório GSouza, Carolina CMelo, Maria NPinto, Sebastião AQuixabeira, Valeria BPereira, Ledice IDorta, Miriam LRibeiro-Dias, FatimaSilveira, Fernando TobiasSilva, Sydnei MGontijo, Celia MTafuri, Wagner L2017-11-14T11:46:59Z2017-11-14T11:46:59Z2017MARQUES, Fernanda A. et al. Effectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis. Parasitology International, v. 66, n. 1, p. 884-888, 2017.1383-5769https://patua.iec.gov.br/handle/iec/284410.1016/j.parint.2016.10.003American tegumentary leishmaniasis (ATL) is a neglected disease widely distributed in Latin America. In Brazil, it is caused by different Leishmania species belonging to the Subgenera Viannia and Leishmania. ATL diagnosis is routinely based on clinical, epidemiological, parasitological and immunological (delayed-type hypersensitivity skin test-DTH) evidences. The main objective of this work was to determine the efficacy of a previous immunohistochemical (IHC) method developed by our group. Seventy eight skin biopsies from patients with different ATL clinical forms and origins were evaluated. The method was previously standardized in ATL patients from the municipality of Caratinga, Minas Gerais, Brazil, all infected with Leishmania (V.) braziliensis. Here, it is evaluated in patients from the North, Southeast and Midwest regions of Brazil. Clinical, parasitological (biopsy PCR) and immunological (Montenegro skin test-MST) diagnosis were performed prior to IHC procedure. The IHC procedure detected 70.5% of the cases having a high agreement with MST diagnosis (kappa=0.84). A distinguished contribution of this work is that IHC succeed in diagnosing some negative DTH patients. Those were infected with Leishmania (L.) amazonensis, commonly causing the anergic form of the disease. In conclusion, IHC succeed in detecting ATL caused by different Leishmania species from various geographic regions and clinical status. Although it was not able to detect ATL in all patients, it was better than MST providing an additional tool for the diagnosis of ATL patients. There was no significant correlation between clinical forms and histological features including the presence of necrosisUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil.Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia, GO, Brazil.Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia, GO, Brazil.Universidade Federal de Goiás. Faculdade de Medicina. Instituto Goiano de Oncologia e Hematologia. Goiânia, GO, Brazil.Universidade Federal de Goiás. Faculdade de Medicina. Instituto Goiano de Oncologia e Hematologia. Goiânia, GO, Brazil.Universidade Federal de Goiás. Faculdade de Medicina. Instituto Goiano de Oncologia e Hematologia. Goiânia, GO, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brazil.Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Departamento de Imunologia, Microbiologia e Parasitologia. Uberlândia, MG, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Belo Horizonte, MG, Brazil.engElsevierEffectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLeishmania / imunologiaLeishmania / isolamento & purificaçãoLeishmania braziliensis / imunologiaLeishmania braziliensis / isolamento & purificaçãoLeishmaniose Cutânea / diagnósticoLeishmaniose Cutânea / epidemiologiaLeishmaniose Cutânea / parasitologiaPele / parasitologiaPele / patologiaImuno-Histoquímica / métodosBrasil / epidemiologiaEstados Unidosinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALEffectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis.pdfEffectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis.pdfapplication/pdf1911055https://patua.iec.gov.br/bitstreams/adb67f40-e0ed-4869-af59-19677a423af5/download3dda3167149df2358b5e0d5d21b5bc38MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/089c9fc6-ec78-407b-9cb0-2f6b2e8c7736/download52f1732ea66fbd1123abe39f5373b797MD52TEXTEffectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis.pdf.txtEffectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis.pdf.txtExtracted texttext/plain28713https://patua.iec.gov.br/bitstreams/e1922fce-b3df-42c6-8178-ee410912753f/downloada17e4c007e26e15b878d483ea6b378e2MD55THUMBNAILEffectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis.pdf.jpgEffectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis.pdf.jpgGenerated Thumbnailimage/jpeg5441https://patua.iec.gov.br/bitstreams/76bf75ee-544c-40c0-a0e6-2c572da812a5/download8b10cd205329f5d6da225fef2fe11761MD56iec/28442023-05-10 11:42:07.08oai:patua.iec.gov.br:iec/2844https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2023-05-10T11:42:07Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4= |
dc.title.en_US.fl_str_mv |
Effectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis |
title |
Effectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis |
spellingShingle |
Effectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis Marques, Fernanda A Leishmania / imunologia Leishmania / isolamento & purificação Leishmania braziliensis / imunologia Leishmania braziliensis / isolamento & purificação Leishmaniose Cutânea / diagnóstico Leishmaniose Cutânea / epidemiologia Leishmaniose Cutânea / parasitologia Pele / parasitologia Pele / patologia Imuno-Histoquímica / métodos Brasil / epidemiologia Estados Unidos |
title_short |
Effectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis |
title_full |
Effectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis |
title_fullStr |
Effectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis |
title_full_unstemmed |
Effectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis |
title_sort |
Effectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis |
author |
Marques, Fernanda A |
author_facet |
Marques, Fernanda A Soares, Rodrigo P Almeida, Gregório G Souza, Carolina C Melo, Maria N Pinto, Sebastião A Quixabeira, Valeria B Pereira, Ledice I Dorta, Miriam L Ribeiro-Dias, Fatima Silveira, Fernando Tobias Silva, Sydnei M Gontijo, Celia M Tafuri, Wagner L |
author_role |
author |
author2 |
Soares, Rodrigo P Almeida, Gregório G Souza, Carolina C Melo, Maria N Pinto, Sebastião A Quixabeira, Valeria B Pereira, Ledice I Dorta, Miriam L Ribeiro-Dias, Fatima Silveira, Fernando Tobias Silva, Sydnei M Gontijo, Celia M Tafuri, Wagner L |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Marques, Fernanda A Soares, Rodrigo P Almeida, Gregório G Souza, Carolina C Melo, Maria N Pinto, Sebastião A Quixabeira, Valeria B Pereira, Ledice I Dorta, Miriam L Ribeiro-Dias, Fatima Silveira, Fernando Tobias Silva, Sydnei M Gontijo, Celia M Tafuri, Wagner L |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Leishmania / imunologia Leishmania / isolamento & purificação Leishmania braziliensis / imunologia Leishmania braziliensis / isolamento & purificação Leishmaniose Cutânea / diagnóstico Leishmaniose Cutânea / epidemiologia Leishmaniose Cutânea / parasitologia Pele / parasitologia Pele / patologia Imuno-Histoquímica / métodos Brasil / epidemiologia |
topic |
Leishmania / imunologia Leishmania / isolamento & purificação Leishmania braziliensis / imunologia Leishmania braziliensis / isolamento & purificação Leishmaniose Cutânea / diagnóstico Leishmaniose Cutânea / epidemiologia Leishmaniose Cutânea / parasitologia Pele / parasitologia Pele / patologia Imuno-Histoquímica / métodos Brasil / epidemiologia Estados Unidos |
dc.subject.decsPrimary.en_US.fl_str_mv |
Estados Unidos |
description |
American tegumentary leishmaniasis (ATL) is a neglected disease widely distributed in Latin America. In Brazil, it is caused by different Leishmania species belonging to the Subgenera Viannia and Leishmania. ATL diagnosis is routinely based on clinical, epidemiological, parasitological and immunological (delayed-type hypersensitivity skin test-DTH) evidences. The main objective of this work was to determine the efficacy of a previous immunohistochemical (IHC) method developed by our group. Seventy eight skin biopsies from patients with different ATL clinical forms and origins were evaluated. The method was previously standardized in ATL patients from the municipality of Caratinga, Minas Gerais, Brazil, all infected with Leishmania (V.) braziliensis. Here, it is evaluated in patients from the North, Southeast and Midwest regions of Brazil. Clinical, parasitological (biopsy PCR) and immunological (Montenegro skin test-MST) diagnosis were performed prior to IHC procedure. The IHC procedure detected 70.5% of the cases having a high agreement with MST diagnosis (kappa=0.84). A distinguished contribution of this work is that IHC succeed in diagnosing some negative DTH patients. Those were infected with Leishmania (L.) amazonensis, commonly causing the anergic form of the disease. In conclusion, IHC succeed in detecting ATL caused by different Leishmania species from various geographic regions and clinical status. Although it was not able to detect ATL in all patients, it was better than MST providing an additional tool for the diagnosis of ATL patients. There was no significant correlation between clinical forms and histological features including the presence of necrosis |
publishDate |
2017 |
dc.date.accessioned.fl_str_mv |
2017-11-14T11:46:59Z |
dc.date.available.fl_str_mv |
2017-11-14T11:46:59Z |
dc.date.issued.fl_str_mv |
2017 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MARQUES, Fernanda A. et al. Effectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis. Parasitology International, v. 66, n. 1, p. 884-888, 2017. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/2844 |
dc.identifier.issn.none.fl_str_mv |
1383-5769 |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.parint.2016.10.003 |
identifier_str_mv |
MARQUES, Fernanda A. et al. Effectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis. Parasitology International, v. 66, n. 1, p. 884-888, 2017. 1383-5769 10.1016/j.parint.2016.10.003 |
url |
https://patua.iec.gov.br/handle/iec/2844 |
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eng |
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eng |
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info:eu-repo/semantics/embargoedAccess |
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Elsevier |
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Elsevier |
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