Sequencing and description of the complete mitochondrial genome of Limatus durhamii (Diptera: Culicidae)

• Autor(a) principal: Silva, Fábio Silva da
• Data de Publicação: 2023
• Outros Autores: Nascimento, Bruna Laís Sena do, Cruz, Ana Cecília Ribeiro, Silva, Sandro Patroca da, Aragão, Andressa de Oliveira, Dias, Daniel Damous, Silva, Lucas Henrique da Silva e, Reis, Lúcia Aline Moura, Rosa, José Wilson, Vieira, Durval Bertram Rodrigues, Medeiros, Daniele Barbosa de Almeida, Nunes Neto, Joaquim Pinto
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/6738
• topic: Culicidae / crescimento & desenvolvimento; Culicidae / classificação; Limatus durhamii / classificação; Genoma Mitocondrial / genética; Sequenciamento Completo do Genoma / métodos

The genus Limatus (Diptera: Culicidae) are wild mosquitoes belonging to the Sabethini tribe that occurs in tropical countries and is related to transmission cycles of Orthobunyavirus (Bunyaviridae), particularly in the Amazon region. Given the unavailability of information related to evolutionary biology and molecular taxonomy aspects of this genus, we report here the first complete sequencing of the mitochondrial genome of Limatus durhamii Theobald, 1901. The NextSeq 500 platform was used for sample sequencing, and the mitochondrial sequence obtained was 14,875 bp long, comprising 37 functional subunits (13 PCGs, 22 tRNA and 02 rRNA). The phylogeny reconstructed by maximum likelihood based on the concatenation of all 13 PCGs corroborated the known taxonomic classification based most on aspects of the external morphology and few molecular studies. The data and information produced here may be useful in the future development of taxonomic and evolutionary studies for the genus, as well as the Culicidae family itself.