Phylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensin

Detalhes bibliográficos
Autor(a) principal: Santos, Erian de Almeida
Data de Publicação: 2022
Outros Autores: Santos, Ana Cecília Feio dos, Silva, Fábio Silva da, Queiroz, Alice Louize Nunes, Pires, Luciana Letícia da Costa, Casseb, Samir Mansour Moraes, Holanda, Gustavo Moraes, Sucupira, Izis Mônica Carvalho, Cruz, Ana Cecília Ribeiro, Santos, Eduardo José Melo dos, Póvoa, Marinete Marins
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: http://patua.iec.gov.br//handle/iec/4548
Resumo: Cecropins and defensins are the main classes of antimicrobial peptides in the mosquito innate immune system, acting against bacteria, fungi and protozoa. There is a knowledge gap concerning these peptide genes in anopheline mosquitoes from the Brazilian Amazon. Thus, this work aimed to describe molecular techniques for detecting the genes encoding the antimicrobial peptides cecropin A (CecA) and defensin in Anopheles darlingi mosquitoes and to perform molecular phylogeny of the sequenced genes using the maximum likelihood method and Bayesian inference with other species from different geographic areas. Our results show, for the first time, a molecular biology method for detecting CecA and defensin in Anopheles darlingi that allows for the use of these molecular markers for phylogenetic analysis in anopheline species, separating the species into single and monophyletic clades.
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spelling Santos, Erian de AlmeidaSantos, Ana Cecília Feio dosSilva, Fábio Silva daQueiroz, Alice Louize NunesPires, Luciana Letícia da CostaCasseb, Samir Mansour MoraesHolanda, Gustavo MoraesSucupira, Izis Mônica CarvalhoCruz, Ana Cecília RibeiroSantos, Eduardo José Melo dosPóvoa, Marinete Marins2022-06-03T17:18:35Z2022-06-03T17:18:35Z2022SANTOS, Erian de Almeida et al. Phylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensin. Acta Tropica, v. 227, n. 106285, Mar. 2022. DOI: https://doi.org/10.1016/j.actatropica.2021.106285. Acesso em: 03 jun. 2022.0001-706Xhttp://patua.iec.gov.br//handle/iec/454810.1016/j.actatropica.2021.106285Cecropins and defensins are the main classes of antimicrobial peptides in the mosquito innate immune system, acting against bacteria, fungi and protozoa. There is a knowledge gap concerning these peptide genes in anopheline mosquitoes from the Brazilian Amazon. Thus, this work aimed to describe molecular techniques for detecting the genes encoding the antimicrobial peptides cecropin A (CecA) and defensin in Anopheles darlingi mosquitoes and to perform molecular phylogeny of the sequenced genes using the maximum likelihood method and Bayesian inference with other species from different geographic areas. Our results show, for the first time, a molecular biology method for detecting CecA and defensin in Anopheles darlingi that allows for the use of these molecular markers for phylogenetic analysis in anopheline species, separating the species into single and monophyletic clades.This study was financially supported by the National Council for Scientific and Technological Development (CNPq n° 302292/2017–9), the Coordination for the Improvement of Higher Education Personnel (CAPES n° 88882.183965/2018–01), Instituto Evandro Chagas/SVS/MS and Norte Energia SA.Universidade Federal do Pará. Programa de Pós-graduação em Biologia de Agentes Infecciosos e Parasitários. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Entomologia de Malária. Ananindeua, PA, Brasil / Fundação de Vigilância em Saúde do Amazonas - Dra. Rosemary Costa Pinto. Manaus, AM, BrazilUniversidade Federal do Pará. Programa de Pós-graduação em Biologia de Agentes Infecciosos e Parasitários. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Entomologia de Malária. Ananindeua, PA, BrasillMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Entomologia de Malária. Ananindeua, PA, BrasilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Entomologia de Malária. Ananindeua, PA, BrasilUniversidade Federal do Pará. Programa de Pós-graduação em Biologia de Agentes Infecciosos e Parasitários. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Universidade do Estado do Pará. Belém, PA, BrazilUniversidade Federal do Pará. Programa de Pós-graduação em Biologia de Agentes Infecciosos e Parasitários. Belém, PA, BrazilUniversidade Federal do Pará. Programa de Pós-graduação em Biologia de Agentes Infecciosos e Parasitários. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Entomologia de Malária. 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dc.title.pt_BR.fl_str_mv Phylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensin
title Phylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensin
spellingShingle Phylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensin
Santos, Erian de Almeida
Anopheles / anatomia & histologia
Anopheles / classificação
Biologia Molecular / métodos
Filogenia
Peptídeos Antimicrobianos
Defensinas
Cecropinas
title_short Phylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensin
title_full Phylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensin
title_fullStr Phylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensin
title_full_unstemmed Phylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensin
title_sort Phylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensin
author Santos, Erian de Almeida
author_facet Santos, Erian de Almeida
Santos, Ana Cecília Feio dos
Silva, Fábio Silva da
Queiroz, Alice Louize Nunes
Pires, Luciana Letícia da Costa
Casseb, Samir Mansour Moraes
Holanda, Gustavo Moraes
Sucupira, Izis Mônica Carvalho
Cruz, Ana Cecília Ribeiro
Santos, Eduardo José Melo dos
Póvoa, Marinete Marins
author_role author
author2 Santos, Ana Cecília Feio dos
Silva, Fábio Silva da
Queiroz, Alice Louize Nunes
Pires, Luciana Letícia da Costa
Casseb, Samir Mansour Moraes
Holanda, Gustavo Moraes
Sucupira, Izis Mônica Carvalho
Cruz, Ana Cecília Ribeiro
Santos, Eduardo José Melo dos
Póvoa, Marinete Marins
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Erian de Almeida
Santos, Ana Cecília Feio dos
Silva, Fábio Silva da
Queiroz, Alice Louize Nunes
Pires, Luciana Letícia da Costa
Casseb, Samir Mansour Moraes
Holanda, Gustavo Moraes
Sucupira, Izis Mônica Carvalho
Cruz, Ana Cecília Ribeiro
Santos, Eduardo José Melo dos
Póvoa, Marinete Marins
dc.subject.decsPrimary.pt_BR.fl_str_mv Anopheles / anatomia & histologia
Anopheles / classificação
Biologia Molecular / métodos
Filogenia
Peptídeos Antimicrobianos
Defensinas
Cecropinas
topic Anopheles / anatomia & histologia
Anopheles / classificação
Biologia Molecular / métodos
Filogenia
Peptídeos Antimicrobianos
Defensinas
Cecropinas
description Cecropins and defensins are the main classes of antimicrobial peptides in the mosquito innate immune system, acting against bacteria, fungi and protozoa. There is a knowledge gap concerning these peptide genes in anopheline mosquitoes from the Brazilian Amazon. Thus, this work aimed to describe molecular techniques for detecting the genes encoding the antimicrobial peptides cecropin A (CecA) and defensin in Anopheles darlingi mosquitoes and to perform molecular phylogeny of the sequenced genes using the maximum likelihood method and Bayesian inference with other species from different geographic areas. Our results show, for the first time, a molecular biology method for detecting CecA and defensin in Anopheles darlingi that allows for the use of these molecular markers for phylogenetic analysis in anopheline species, separating the species into single and monophyletic clades.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-06-03T17:18:35Z
dc.date.available.fl_str_mv 2022-06-03T17:18:35Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, Erian de Almeida et al. Phylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensin. Acta Tropica, v. 227, n. 106285, Mar. 2022. DOI: https://doi.org/10.1016/j.actatropica.2021.106285. Acesso em: 03 jun. 2022.
dc.identifier.uri.fl_str_mv http://patua.iec.gov.br//handle/iec/4548
dc.identifier.issn.pt_BR.fl_str_mv 0001-706X
dc.identifier.doi.pt_BR.fl_str_mv 10.1016/j.actatropica.2021.106285
identifier_str_mv SANTOS, Erian de Almeida et al. Phylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensin. Acta Tropica, v. 227, n. 106285, Mar. 2022. DOI: https://doi.org/10.1016/j.actatropica.2021.106285. Acesso em: 03 jun. 2022.
0001-706X
10.1016/j.actatropica.2021.106285
url http://patua.iec.gov.br//handle/iec/4548
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
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