Phylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensin
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | http://patua.iec.gov.br//handle/iec/4548 |
Resumo: | Cecropins and defensins are the main classes of antimicrobial peptides in the mosquito innate immune system, acting against bacteria, fungi and protozoa. There is a knowledge gap concerning these peptide genes in anopheline mosquitoes from the Brazilian Amazon. Thus, this work aimed to describe molecular techniques for detecting the genes encoding the antimicrobial peptides cecropin A (CecA) and defensin in Anopheles darlingi mosquitoes and to perform molecular phylogeny of the sequenced genes using the maximum likelihood method and Bayesian inference with other species from different geographic areas. Our results show, for the first time, a molecular biology method for detecting CecA and defensin in Anopheles darlingi that allows for the use of these molecular markers for phylogenetic analysis in anopheline species, separating the species into single and monophyletic clades. |
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Santos, Erian de AlmeidaSantos, Ana Cecília Feio dosSilva, Fábio Silva daQueiroz, Alice Louize NunesPires, Luciana Letícia da CostaCasseb, Samir Mansour MoraesHolanda, Gustavo MoraesSucupira, Izis Mônica CarvalhoCruz, Ana Cecília RibeiroSantos, Eduardo José Melo dosPóvoa, Marinete Marins2022-06-03T17:18:35Z2022-06-03T17:18:35Z2022SANTOS, Erian de Almeida et al. Phylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensin. Acta Tropica, v. 227, n. 106285, Mar. 2022. DOI: https://doi.org/10.1016/j.actatropica.2021.106285. Acesso em: 03 jun. 2022.0001-706Xhttp://patua.iec.gov.br//handle/iec/454810.1016/j.actatropica.2021.106285Cecropins and defensins are the main classes of antimicrobial peptides in the mosquito innate immune system, acting against bacteria, fungi and protozoa. There is a knowledge gap concerning these peptide genes in anopheline mosquitoes from the Brazilian Amazon. Thus, this work aimed to describe molecular techniques for detecting the genes encoding the antimicrobial peptides cecropin A (CecA) and defensin in Anopheles darlingi mosquitoes and to perform molecular phylogeny of the sequenced genes using the maximum likelihood method and Bayesian inference with other species from different geographic areas. Our results show, for the first time, a molecular biology method for detecting CecA and defensin in Anopheles darlingi that allows for the use of these molecular markers for phylogenetic analysis in anopheline species, separating the species into single and monophyletic clades.This study was financially supported by the National Council for Scientific and Technological Development (CNPq n° 302292/2017–9), the Coordination for the Improvement of Higher Education Personnel (CAPES n° 88882.183965/2018–01), Instituto Evandro Chagas/SVS/MS and Norte Energia SA.Universidade Federal do Pará. Programa de Pós-graduação em Biologia de Agentes Infecciosos e Parasitários. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Entomologia de Malária. Ananindeua, PA, Brasil / Fundação de Vigilância em Saúde do Amazonas - Dra. Rosemary Costa Pinto. Manaus, AM, BrazilUniversidade Federal do Pará. Programa de Pós-graduação em Biologia de Agentes Infecciosos e Parasitários. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Entomologia de Malária. Ananindeua, PA, BrasillMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Entomologia de Malária. Ananindeua, PA, BrasilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Entomologia de Malária. Ananindeua, PA, BrasilUniversidade Federal do Pará. Programa de Pós-graduação em Biologia de Agentes Infecciosos e Parasitários. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Universidade do Estado do Pará. Belém, PA, BrazilUniversidade Federal do Pará. Programa de Pós-graduação em Biologia de Agentes Infecciosos e Parasitários. Belém, PA, BrazilUniversidade Federal do Pará. Programa de Pós-graduação em Biologia de Agentes Infecciosos e Parasitários. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Entomologia de Malária. Ananindeua, PA, BrasilengElsevierPhylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAnopheles / anatomia & histologiaAnopheles / classificaçãoBiologia Molecular / métodosFilogeniaPeptídeos AntimicrobianosDefensinasCecropinasinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALPhylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensin.pdfPhylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensin.pdfapplication/pdf7142986https://patua.iec.gov.br/bitstreams/4314c230-1d7c-4e56-9a18-5076fe5ebbcf/downloadf719bbf3037de9db9834a5e7350587daMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/3c269294-e898-48aa-b77c-9fda28fd4980/download11832eea31b16df8613079d742d61793MD52TEXTPhylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensin.pdf.txtPhylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensin.pdf.txtExtracted texttext/plain42828https://patua.iec.gov.br/bitstreams/d7d71cb8-841e-4d0e-b0f6-23020529094b/downloadd444743bedbe9c068ee4497c40f6aae3MD55THUMBNAILPhylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensin.pdf.jpgPhylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensin.pdf.jpgGenerated Thumbnailimage/jpeg5824https://patua.iec.gov.br/bitstreams/ccd2447a-a1cf-4b47-8ae9-6bc5fd649fe2/downloadaa51365c1a63e854c3ee77261f44bd0aMD56iec/45482022-10-20 23:25:07.342oai:patua.iec.gov.br:iec/4548https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T23:25:07Repositório Digital do Instituto Evandro Chagas (Patuá) - 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dc.title.pt_BR.fl_str_mv |
Phylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensin |
title |
Phylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensin |
spellingShingle |
Phylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensin Santos, Erian de Almeida Anopheles / anatomia & histologia Anopheles / classificação Biologia Molecular / métodos Filogenia Peptídeos Antimicrobianos Defensinas Cecropinas |
title_short |
Phylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensin |
title_full |
Phylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensin |
title_fullStr |
Phylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensin |
title_full_unstemmed |
Phylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensin |
title_sort |
Phylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensin |
author |
Santos, Erian de Almeida |
author_facet |
Santos, Erian de Almeida Santos, Ana Cecília Feio dos Silva, Fábio Silva da Queiroz, Alice Louize Nunes Pires, Luciana Letícia da Costa Casseb, Samir Mansour Moraes Holanda, Gustavo Moraes Sucupira, Izis Mônica Carvalho Cruz, Ana Cecília Ribeiro Santos, Eduardo José Melo dos Póvoa, Marinete Marins |
author_role |
author |
author2 |
Santos, Ana Cecília Feio dos Silva, Fábio Silva da Queiroz, Alice Louize Nunes Pires, Luciana Letícia da Costa Casseb, Samir Mansour Moraes Holanda, Gustavo Moraes Sucupira, Izis Mônica Carvalho Cruz, Ana Cecília Ribeiro Santos, Eduardo José Melo dos Póvoa, Marinete Marins |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Santos, Erian de Almeida Santos, Ana Cecília Feio dos Silva, Fábio Silva da Queiroz, Alice Louize Nunes Pires, Luciana Letícia da Costa Casseb, Samir Mansour Moraes Holanda, Gustavo Moraes Sucupira, Izis Mônica Carvalho Cruz, Ana Cecília Ribeiro Santos, Eduardo José Melo dos Póvoa, Marinete Marins |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Anopheles / anatomia & histologia Anopheles / classificação Biologia Molecular / métodos Filogenia Peptídeos Antimicrobianos Defensinas Cecropinas |
topic |
Anopheles / anatomia & histologia Anopheles / classificação Biologia Molecular / métodos Filogenia Peptídeos Antimicrobianos Defensinas Cecropinas |
description |
Cecropins and defensins are the main classes of antimicrobial peptides in the mosquito innate immune system, acting against bacteria, fungi and protozoa. There is a knowledge gap concerning these peptide genes in anopheline mosquitoes from the Brazilian Amazon. Thus, this work aimed to describe molecular techniques for detecting the genes encoding the antimicrobial peptides cecropin A (CecA) and defensin in Anopheles darlingi mosquitoes and to perform molecular phylogeny of the sequenced genes using the maximum likelihood method and Bayesian inference with other species from different geographic areas. Our results show, for the first time, a molecular biology method for detecting CecA and defensin in Anopheles darlingi that allows for the use of these molecular markers for phylogenetic analysis in anopheline species, separating the species into single and monophyletic clades. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-06-03T17:18:35Z |
dc.date.available.fl_str_mv |
2022-06-03T17:18:35Z |
dc.date.issued.fl_str_mv |
2022 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SANTOS, Erian de Almeida et al. Phylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensin. Acta Tropica, v. 227, n. 106285, Mar. 2022. DOI: https://doi.org/10.1016/j.actatropica.2021.106285. Acesso em: 03 jun. 2022. |
dc.identifier.uri.fl_str_mv |
http://patua.iec.gov.br//handle/iec/4548 |
dc.identifier.issn.pt_BR.fl_str_mv |
0001-706X |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1016/j.actatropica.2021.106285 |
identifier_str_mv |
SANTOS, Erian de Almeida et al. Phylogeny of Anopheles darlingi (Diptera:Culicidae) based on the antimicrobial peptide genes cecropin and defensin. Acta Tropica, v. 227, n. 106285, Mar. 2022. DOI: https://doi.org/10.1016/j.actatropica.2021.106285. Acesso em: 03 jun. 2022. 0001-706X 10.1016/j.actatropica.2021.106285 |
url |
http://patua.iec.gov.br//handle/iec/4548 |
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eng |
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eng |
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Elsevier |
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Elsevier |
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