The isotype composition and avidity of naturally acquired anti-plasmodium falciparum antibodies: differential patterns in clinically immune africans and amazonian patients

• Autor(a) principal: Ferreira, Marcelo Urbano
• Data de Publicação: 1996
• Outros Autores: Kimura, Emilia Akemi Shiraishi, Souza, José Maria de, Katzin, Alejandro Miguel
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/3563
• topic: Malária; Plasmodium falciparum / parasitologia; Técnicas Imunoenzimáticas / métodos

A critical role has been proposed for cytophilic IgOl and IgOJ subclass antibodies and monocytes and macrophages in antimalarial immunity. Here we compared lhe isotype composition and avidity of naturally acquired antibodies, as measured by enzyme immunoassay against a detergent-soluble extract of Plasmodium falciparum schizonts, in clinically immune Senegalese adults (n = 33) and semi-immune, adult Amazonian patients (n = 25). Plasma were collected during an acute symptomatic P. falciparum attack and two months later, and in the absence of recrudescence or reinfection. Specific IgO, IgM, IgA, and IgO subclass antibodies were assessed. The results are summarized as follows: 1) high-avidity cytophilic antibodies predorninated in clinically immune Senegalese subjects; 2) acutely ill Amazonian patients produced high levels of low-avidity cytophilic antibody; 3) such a response was shonlived, since two months later, the concentrations of cytophilic antibodies were significantly lower; 4) however, affinity maturation of IgO antibodies was observed in Amazonian patients two months after the acute malaria attack. A considerable proponion (35-46%) of anti-P. falciparum IgOI antibodies produced by African and Amazonian patients was shown to recognize periodate-sensitive carbohydrate epitopes. The potential impact of these findings on the design and evaluation of antimalarial vaccines is discussed.