Clinical and molecular diagnosis of Chlamydophila in captive parrots in Pará State, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/3966 |
Resumo: | Parrots (Order Psittaciformes) are globally distributed birds that, together with members of the Columbiformes, represent the most susceptible animals, in regards to infection by Chlamydophila psittaci, which is an obligate, zoonotic, intracellular bacterium that causes chlamydiosis in domestic and wild birds and psittacosis in humans. The aim of the present study was to assess the occurrence of C. psittaci in Brazilian psittacids kept in conservation breeding sites in Pará State, Brazil. Cloacal and oropharynx swab samples were collected from 201 psittacids that were distributed among four breeding sites: Metropolitan Area of Belém (C1 and C2), Northeastern Pará (C3), and Low Amazon (C4). The samples were screened for C. psittaci using semi-nested PCR, and the resulting incidence data were analyzed using proportion and chi-square tests. Chlamydophila infection was confirmed for all the breeding sites, with an overall prevalence of 31.84%, and no species-specific predisposition was observed. Furthermore, 13.93% of the sampled birds eliminated the infectious agent by the cloaca, whereas 11.44% eliminated the agent by the oropharynx, and 6.47% eliminated the agent by both routes. Moreover, there was a significant difference between the incidence of Chlamydophila infection of breeding sites C2 and C3 (p=0.029), which yielded the smallest and largest number of diagnosed cases, respectively. In the present study, most of the birds (27.86%) were considered unapparent carriers of Chlamydophila infection, and only 3.98% of the birds yielded both a positive diagnosis and clinical signs of chlamydiosis. |
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Araújo, Simone Aparecida AlmeidaPereira, Washington Luiz AssunçãoSilva, Sandro Patroca daCardoso, Jedson FerreiraSilva Filho, Ednaldo daBernal, Marcella Katheryne MarquesMendes, Fernanda FigueiredoNunes, Márcio Roberto Teixeira2019-11-12T12:25:14Z2019-11-12T12:25:14Z2019ARAÚJO, Simone Aparecida Almeida et al. Clinical and molecular diagnosis of Chlamydophila in captive parrots in Pará State, Brazil. Semina: Ciências Agrárias, v. 40, n. 6, p. 2603-2612, nov./dez. 2019.1679-0359https://patua.iec.gov.br/handle/iec/396610.5433/1679-0359.2019v40n6p2603Parrots (Order Psittaciformes) are globally distributed birds that, together with members of the Columbiformes, represent the most susceptible animals, in regards to infection by Chlamydophila psittaci, which is an obligate, zoonotic, intracellular bacterium that causes chlamydiosis in domestic and wild birds and psittacosis in humans. The aim of the present study was to assess the occurrence of C. psittaci in Brazilian psittacids kept in conservation breeding sites in Pará State, Brazil. Cloacal and oropharynx swab samples were collected from 201 psittacids that were distributed among four breeding sites: Metropolitan Area of Belém (C1 and C2), Northeastern Pará (C3), and Low Amazon (C4). The samples were screened for C. psittaci using semi-nested PCR, and the resulting incidence data were analyzed using proportion and chi-square tests. Chlamydophila infection was confirmed for all the breeding sites, with an overall prevalence of 31.84%, and no species-specific predisposition was observed. Furthermore, 13.93% of the sampled birds eliminated the infectious agent by the cloaca, whereas 11.44% eliminated the agent by the oropharynx, and 6.47% eliminated the agent by both routes. Moreover, there was a significant difference between the incidence of Chlamydophila infection of breeding sites C2 and C3 (p=0.029), which yielded the smallest and largest number of diagnosed cases, respectively. In the present study, most of the birds (27.86%) were considered unapparent carriers of Chlamydophila infection, and only 3.98% of the birds yielded both a positive diagnosis and clinical signs of chlamydiosis.Instituto Federal de Educação, Ciência e Tecnologia do Pará. Marabá, PA, Brasil.Universidade Federal Rural da Amazônia. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Federal Rural da Amazônia. Belém, PA, Brasil.Universidade Federal Rural da Amazônia. Belém, PA, Brasil.Universidade Federal Rural da Amazônia. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.engUniversidade Estadual de LondrinaClinical and molecular diagnosis of Chlamydophila in captive parrots in Pará State, BrazilEstudo clínico e diagnóstico molecular de Chlamydophila sp. em psitaciformes mantidos em cativeiro no Estado do Pará, Brasilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePapagaios / anatomia & histologiaInfecções por Chlamydophila / veterináriaChlamydophila psittaciInfecções por Chlamydia / veterináriaReação em Cadeia da Polimerase / métodosinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALClinical and molecular diagnosis of Chlamydophila in captive parrots in Pará State, Brazil.pdfClinical and molecular diagnosis of Chlamydophila in captive parrots in Pará State, Brazil.pdfapplication/pdf640843https://patua.iec.gov.br/bitstreams/8f621a53-8c21-4f28-8c91-91a03d3338c8/downloada88e15da0d7fb8fea9358524e30308c7MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/f176c565-ac5c-435b-abd8-ce3f189ac8ee/download11832eea31b16df8613079d742d61793MD52TEXTClinical and molecular diagnosis of Chlamydophila in captive parrots in Pará State, Brazil.pdf.txtClinical and molecular diagnosis of Chlamydophila in captive parrots in Pará State, Brazil.pdf.txtExtracted texttext/plain36904https://patua.iec.gov.br/bitstreams/55866f52-efbe-4cfd-a979-d051fbc04fd0/download319a825cd0218c4c52c47ab66302ac43MD55THUMBNAILClinical and molecular diagnosis of Chlamydophila in captive parrots in Pará State, Brazil.pdf.jpgClinical and molecular diagnosis of Chlamydophila in captive parrots in Pará State, Brazil.pdf.jpgGenerated Thumbnailimage/jpeg5636https://patua.iec.gov.br/bitstreams/4c1d9a52-ca2f-4678-8008-de2bd93c7d87/download0cae82b7d19638f463fdc011ee4a87e3MD56iec/39662022-10-20 22:47:04.452oai:patua.iec.gov.br:iec/3966https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T22:47:04Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)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 |
dc.title.pt_BR.fl_str_mv |
Clinical and molecular diagnosis of Chlamydophila in captive parrots in Pará State, Brazil |
dc.title.alternative.pt_BR.fl_str_mv |
Estudo clínico e diagnóstico molecular de Chlamydophila sp. em psitaciformes mantidos em cativeiro no Estado do Pará, Brasil |
title |
Clinical and molecular diagnosis of Chlamydophila in captive parrots in Pará State, Brazil |
spellingShingle |
Clinical and molecular diagnosis of Chlamydophila in captive parrots in Pará State, Brazil Araújo, Simone Aparecida Almeida Papagaios / anatomia & histologia Infecções por Chlamydophila / veterinária Chlamydophila psittaci Infecções por Chlamydia / veterinária Reação em Cadeia da Polimerase / métodos |
title_short |
Clinical and molecular diagnosis of Chlamydophila in captive parrots in Pará State, Brazil |
title_full |
Clinical and molecular diagnosis of Chlamydophila in captive parrots in Pará State, Brazil |
title_fullStr |
Clinical and molecular diagnosis of Chlamydophila in captive parrots in Pará State, Brazil |
title_full_unstemmed |
Clinical and molecular diagnosis of Chlamydophila in captive parrots in Pará State, Brazil |
title_sort |
Clinical and molecular diagnosis of Chlamydophila in captive parrots in Pará State, Brazil |
author |
Araújo, Simone Aparecida Almeida |
author_facet |
Araújo, Simone Aparecida Almeida Pereira, Washington Luiz Assunção Silva, Sandro Patroca da Cardoso, Jedson Ferreira Silva Filho, Ednaldo da Bernal, Marcella Katheryne Marques Mendes, Fernanda Figueiredo Nunes, Márcio Roberto Teixeira |
author_role |
author |
author2 |
Pereira, Washington Luiz Assunção Silva, Sandro Patroca da Cardoso, Jedson Ferreira Silva Filho, Ednaldo da Bernal, Marcella Katheryne Marques Mendes, Fernanda Figueiredo Nunes, Márcio Roberto Teixeira |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Araújo, Simone Aparecida Almeida Pereira, Washington Luiz Assunção Silva, Sandro Patroca da Cardoso, Jedson Ferreira Silva Filho, Ednaldo da Bernal, Marcella Katheryne Marques Mendes, Fernanda Figueiredo Nunes, Márcio Roberto Teixeira |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Papagaios / anatomia & histologia Infecções por Chlamydophila / veterinária Chlamydophila psittaci Infecções por Chlamydia / veterinária Reação em Cadeia da Polimerase / métodos |
topic |
Papagaios / anatomia & histologia Infecções por Chlamydophila / veterinária Chlamydophila psittaci Infecções por Chlamydia / veterinária Reação em Cadeia da Polimerase / métodos |
description |
Parrots (Order Psittaciformes) are globally distributed birds that, together with members of the Columbiformes, represent the most susceptible animals, in regards to infection by Chlamydophila psittaci, which is an obligate, zoonotic, intracellular bacterium that causes chlamydiosis in domestic and wild birds and psittacosis in humans. The aim of the present study was to assess the occurrence of C. psittaci in Brazilian psittacids kept in conservation breeding sites in Pará State, Brazil. Cloacal and oropharynx swab samples were collected from 201 psittacids that were distributed among four breeding sites: Metropolitan Area of Belém (C1 and C2), Northeastern Pará (C3), and Low Amazon (C4). The samples were screened for C. psittaci using semi-nested PCR, and the resulting incidence data were analyzed using proportion and chi-square tests. Chlamydophila infection was confirmed for all the breeding sites, with an overall prevalence of 31.84%, and no species-specific predisposition was observed. Furthermore, 13.93% of the sampled birds eliminated the infectious agent by the cloaca, whereas 11.44% eliminated the agent by the oropharynx, and 6.47% eliminated the agent by both routes. Moreover, there was a significant difference between the incidence of Chlamydophila infection of breeding sites C2 and C3 (p=0.029), which yielded the smallest and largest number of diagnosed cases, respectively. In the present study, most of the birds (27.86%) were considered unapparent carriers of Chlamydophila infection, and only 3.98% of the birds yielded both a positive diagnosis and clinical signs of chlamydiosis. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-11-12T12:25:14Z |
dc.date.available.fl_str_mv |
2019-11-12T12:25:14Z |
dc.date.issued.fl_str_mv |
2019 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ARAÚJO, Simone Aparecida Almeida et al. Clinical and molecular diagnosis of Chlamydophila in captive parrots in Pará State, Brazil. Semina: Ciências Agrárias, v. 40, n. 6, p. 2603-2612, nov./dez. 2019. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/3966 |
dc.identifier.issn.-.fl_str_mv |
1679-0359 |
dc.identifier.doi.-.fl_str_mv |
10.5433/1679-0359.2019v40n6p2603 |
identifier_str_mv |
ARAÚJO, Simone Aparecida Almeida et al. Clinical and molecular diagnosis of Chlamydophila in captive parrots in Pará State, Brazil. Semina: Ciências Agrárias, v. 40, n. 6, p. 2603-2612, nov./dez. 2019. 1679-0359 10.5433/1679-0359.2019v40n6p2603 |
url |
https://patua.iec.gov.br/handle/iec/3966 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Londrina |
publisher.none.fl_str_mv |
Universidade Estadual de Londrina |
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reponame:Repositório Digital do Instituto Evandro Chagas (Patuá) instname:Instituto Evandro Chagas (IEC) instacron:IEC |
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Instituto Evandro Chagas (IEC) |
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IEC |
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IEC |
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Repositório Digital do Instituto Evandro Chagas (Patuá) |
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Repositório Digital do Instituto Evandro Chagas (Patuá) |
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