Can domestic dogs be considered a good reservoir of Leishmania (L.) infantum chagasi in an endemic area of nonulcerated cutaneous leishmaniasis in Southern Honduras?
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/6800 |
Resumo: | Dogs are considered to be the main domestic reservoir associated with the transmission of Leishmania (L.) infantum chagasi to humans in endemic areas of visceral leishmaniasis in America. However, little is known about the role of canines as a source of infection in endemic areas of nonulcerated cutaneous leishmaniasis (NUCL). Therefore, the objective of the present study was to investigate the role of dogs as a possible reservoir of the parasite in Southern Honduras. Dogs (n = 107) living with individuals affected by NUCL were clinically examined and biological material was collected for parasitological and immunological diagnosis. Most animals showed a healthy appearance and a few presented slight weight loss (64%), alopecia (7%), onychogryphosis (5%) and skin lesions (1%). The overall seroprevalence of Leishmania infection based on the DDP® quick test and/or in-house ELISA serological test was 41%. The presence of the parasite’s DNA was confirmed in 94% of the dogs; however, the average parasite load in the buffy coat was low at 6.09 parasites/µL, ranging between 0.221 and 50.2. The skin of seropositive dogs examined by histopathology using paraffin sections stained by hematoxylin and immunohistochemistry did not show cutaneous lesions or parasite amastigotes. Based on the absence of parasites in the skin and the low parasite load detected in the buffy coat, it seems that the dog does not represent a good source of infection for the vector in the endemic area of NUCL transmission in Southern Honduras. Other domestic and/or wild animals should be investigated. |
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Segura, Gabriela Beatriz RodriguezOchoa, Wilfredo Humberto SosaMatta, Vânia Lúcia Ribeiro daMartínez, MercedesTercero, Carol RodriguezGonzalez, Raquel RomeroPacheco, Carmen M. SandovalFlores, Gabriela V. AraújoSilveira, Fernando TobiasHenriquez, Maria Mercedes RuedaLaurenti, Márcia Dalastra2023-04-17T19:26:58Z2023-04-17T19:26:58Z2023SEGURA, Gabriela Beatriz Rodriguez et al. Can domestic dogs be considered a good reservoir of Leishmania (L.) infantum chagasi in an endemic area of nonulcerated cutaneous leishmaniasis in Southern Honduras? Revista do Instituto de Medicina Tropical de São Paulo, v. 65, n. e24, p. 1-8, 2023. DOI: https://doi.org/10.1590/S1678-9946202365024. Disponível em: https://www.scielo.br/j/rimtsp/a/JvGW9HtnMmdKmtNK9fNQ95C/1678-9946https://patua.iec.gov.br/handle/iec/680010.1590/S1678-9946202365024Dogs are considered to be the main domestic reservoir associated with the transmission of Leishmania (L.) infantum chagasi to humans in endemic areas of visceral leishmaniasis in America. However, little is known about the role of canines as a source of infection in endemic areas of nonulcerated cutaneous leishmaniasis (NUCL). Therefore, the objective of the present study was to investigate the role of dogs as a possible reservoir of the parasite in Southern Honduras. Dogs (n = 107) living with individuals affected by NUCL were clinically examined and biological material was collected for parasitological and immunological diagnosis. Most animals showed a healthy appearance and a few presented slight weight loss (64%), alopecia (7%), onychogryphosis (5%) and skin lesions (1%). The overall seroprevalence of Leishmania infection based on the DDP® quick test and/or in-house ELISA serological test was 41%. The presence of the parasite’s DNA was confirmed in 94% of the dogs; however, the average parasite load in the buffy coat was low at 6.09 parasites/µL, ranging between 0.221 and 50.2. The skin of seropositive dogs examined by histopathology using paraffin sections stained by hematoxylin and immunohistochemistry did not show cutaneous lesions or parasite amastigotes. Based on the absence of parasites in the skin and the low parasite load detected in the buffy coat, it seems that the dog does not represent a good source of infection for the vector in the endemic area of NUCL transmission in Southern Honduras. Other domestic and/or wild animals should be investigated.This study was supported by the FAPESP, grant Nº 2014/50315-0; CNPq grant Nº 308817/2021-4; DICIHTUNAH grant Nº PI-195-DICIHT; DSEP-UNAH and LIM50 HC-FMUSPUniversidad Nacional Autónoma de Honduras. Instituto de Investigaciones en Microbiologia. Tegucigalpa, Honduras / Universidad Nacional Autónoma de Honduras. Posgrado en Salud Pública. Tegucigalpa, Honduras.Universidad Nacional Autónoma de Honduras. Instituto de Investigaciones en Microbiologia. Tegucigalpa, Honduras.Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. Laboratório de Investigação Médica. São Paulo, SP, Brazil.Universidad Nacional Autónoma de Honduras. Posgrado en Salud Pública. Tegucigalpa, Honduras.Universidad Nacional Autónoma de Honduras. Instituto de Investigaciones en Microbiologia. Tegucigalpa, HondurasUniversidad Nacional Autónoma de Honduras. Instituto de Investigaciones en Microbiologia. Tegucigalpa, HondurasUniversidade de São Paulo. Faculdade de Medicina. Laboratório de Patologia de Moléstias Infecciosas. São Paulo, SP, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Laboratório de Leishmanioses. Ananindeua, PA, Brasil / Universidade Federal do Pará. Núcleo de Medicina Tropical. Belém, PA, Brazil.Universidad Nacional Autónoma de Honduras. Instituto de Investigaciones en Microbiologia. Tegucigalpa, Honduras.Universidade de São Paulo. Faculdade de Medicina. Laboratório de Patologia de Moléstias Infecciosas. São Paulo, SP, Brazil.Universidade de São Paulo. Faculdade de Medicina. Laboratório de Patologia de Moléstias Infecciosas. São Paulo, SP, Brazil.engSociedade Brasileira de Medicina TropicalCan domestic dogs be considered a good reservoir of Leishmania (L.) infantum chagasi in an endemic area of nonulcerated cutaneous leishmaniasis in Southern Honduras?info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLeishmaniose / parasitologiaLeishmania infantum / patogenicidadeCães / parasitologiaCães / lesõesReservatórios de DoençasVetores de DoençasHonduras (HN)info:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALCan domestic dogs be considered a good reservoir of Leishmania (L.) infantum chagasi in an endemic area of nonulcerated cutaneous leishmaniasis in Southern Honduras?.pdfCan domestic dogs be considered a good reservoir of Leishmania (L.) infantum chagasi in an endemic area of nonulcerated cutaneous leishmaniasis in Southern Honduras?.pdfapplication/pdf200897https://patua.iec.gov.br/bitstreams/5c7a1735-b8d9-4cc3-8a0b-d2ed89079d65/download9afdf946601c9ddb14101d3d0fa016efMD51LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv |
Can domestic dogs be considered a good reservoir of Leishmania (L.) infantum chagasi in an endemic area of nonulcerated cutaneous leishmaniasis in Southern Honduras? |
title |
Can domestic dogs be considered a good reservoir of Leishmania (L.) infantum chagasi in an endemic area of nonulcerated cutaneous leishmaniasis in Southern Honduras? |
spellingShingle |
Can domestic dogs be considered a good reservoir of Leishmania (L.) infantum chagasi in an endemic area of nonulcerated cutaneous leishmaniasis in Southern Honduras? Segura, Gabriela Beatriz Rodriguez Leishmaniose / parasitologia Leishmania infantum / patogenicidade Cães / parasitologia Cães / lesões Reservatórios de Doenças Vetores de Doenças Honduras (HN) |
title_short |
Can domestic dogs be considered a good reservoir of Leishmania (L.) infantum chagasi in an endemic area of nonulcerated cutaneous leishmaniasis in Southern Honduras? |
title_full |
Can domestic dogs be considered a good reservoir of Leishmania (L.) infantum chagasi in an endemic area of nonulcerated cutaneous leishmaniasis in Southern Honduras? |
title_fullStr |
Can domestic dogs be considered a good reservoir of Leishmania (L.) infantum chagasi in an endemic area of nonulcerated cutaneous leishmaniasis in Southern Honduras? |
title_full_unstemmed |
Can domestic dogs be considered a good reservoir of Leishmania (L.) infantum chagasi in an endemic area of nonulcerated cutaneous leishmaniasis in Southern Honduras? |
title_sort |
Can domestic dogs be considered a good reservoir of Leishmania (L.) infantum chagasi in an endemic area of nonulcerated cutaneous leishmaniasis in Southern Honduras? |
author |
Segura, Gabriela Beatriz Rodriguez |
author_facet |
Segura, Gabriela Beatriz Rodriguez Ochoa, Wilfredo Humberto Sosa Matta, Vânia Lúcia Ribeiro da Martínez, Mercedes Tercero, Carol Rodriguez Gonzalez, Raquel Romero Pacheco, Carmen M. Sandoval Flores, Gabriela V. Araújo Silveira, Fernando Tobias Henriquez, Maria Mercedes Rueda Laurenti, Márcia Dalastra |
author_role |
author |
author2 |
Ochoa, Wilfredo Humberto Sosa Matta, Vânia Lúcia Ribeiro da Martínez, Mercedes Tercero, Carol Rodriguez Gonzalez, Raquel Romero Pacheco, Carmen M. Sandoval Flores, Gabriela V. Araújo Silveira, Fernando Tobias Henriquez, Maria Mercedes Rueda Laurenti, Márcia Dalastra |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Segura, Gabriela Beatriz Rodriguez Ochoa, Wilfredo Humberto Sosa Matta, Vânia Lúcia Ribeiro da Martínez, Mercedes Tercero, Carol Rodriguez Gonzalez, Raquel Romero Pacheco, Carmen M. Sandoval Flores, Gabriela V. Araújo Silveira, Fernando Tobias Henriquez, Maria Mercedes Rueda Laurenti, Márcia Dalastra |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Leishmaniose / parasitologia Leishmania infantum / patogenicidade Cães / parasitologia Cães / lesões Reservatórios de Doenças Vetores de Doenças Honduras (HN) |
topic |
Leishmaniose / parasitologia Leishmania infantum / patogenicidade Cães / parasitologia Cães / lesões Reservatórios de Doenças Vetores de Doenças Honduras (HN) |
description |
Dogs are considered to be the main domestic reservoir associated with the transmission of Leishmania (L.) infantum chagasi to humans in endemic areas of visceral leishmaniasis in America. However, little is known about the role of canines as a source of infection in endemic areas of nonulcerated cutaneous leishmaniasis (NUCL). Therefore, the objective of the present study was to investigate the role of dogs as a possible reservoir of the parasite in Southern Honduras. Dogs (n = 107) living with individuals affected by NUCL were clinically examined and biological material was collected for parasitological and immunological diagnosis. Most animals showed a healthy appearance and a few presented slight weight loss (64%), alopecia (7%), onychogryphosis (5%) and skin lesions (1%). The overall seroprevalence of Leishmania infection based on the DDP® quick test and/or in-house ELISA serological test was 41%. The presence of the parasite’s DNA was confirmed in 94% of the dogs; however, the average parasite load in the buffy coat was low at 6.09 parasites/µL, ranging between 0.221 and 50.2. The skin of seropositive dogs examined by histopathology using paraffin sections stained by hematoxylin and immunohistochemistry did not show cutaneous lesions or parasite amastigotes. Based on the absence of parasites in the skin and the low parasite load detected in the buffy coat, it seems that the dog does not represent a good source of infection for the vector in the endemic area of NUCL transmission in Southern Honduras. Other domestic and/or wild animals should be investigated. |
publishDate |
2023 |
dc.date.accessioned.fl_str_mv |
2023-04-17T19:26:58Z |
dc.date.available.fl_str_mv |
2023-04-17T19:26:58Z |
dc.date.issued.fl_str_mv |
2023 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
SEGURA, Gabriela Beatriz Rodriguez et al. Can domestic dogs be considered a good reservoir of Leishmania (L.) infantum chagasi in an endemic area of nonulcerated cutaneous leishmaniasis in Southern Honduras? Revista do Instituto de Medicina Tropical de São Paulo, v. 65, n. e24, p. 1-8, 2023. DOI: https://doi.org/10.1590/S1678-9946202365024. Disponível em: https://www.scielo.br/j/rimtsp/a/JvGW9HtnMmdKmtNK9fNQ95C/ |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/6800 |
dc.identifier.issn.-.fl_str_mv |
1678-9946 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1590/S1678-9946202365024 |
identifier_str_mv |
SEGURA, Gabriela Beatriz Rodriguez et al. Can domestic dogs be considered a good reservoir of Leishmania (L.) infantum chagasi in an endemic area of nonulcerated cutaneous leishmaniasis in Southern Honduras? Revista do Instituto de Medicina Tropical de São Paulo, v. 65, n. e24, p. 1-8, 2023. DOI: https://doi.org/10.1590/S1678-9946202365024. Disponível em: https://www.scielo.br/j/rimtsp/a/JvGW9HtnMmdKmtNK9fNQ95C/ 1678-9946 10.1590/S1678-9946202365024 |
url |
https://patua.iec.gov.br/handle/iec/6800 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Sociedade Brasileira de Medicina Tropical |
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Sociedade Brasileira de Medicina Tropical |
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