Occurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | http://patua.iec.gov.br//handle/iec/3642 |
Resumo: | This study aimed to investigate the presence of norovirus (NoV) in recreational waters of four estuarine beaches located in Mosqueiro Island, Belém city, Brazilian Amazon, during two years of monitoring (2012 and 2013). NoV particles were concentrated on filtering membrane by the adsorption-elution method and detected by semi-nested RT-PCR and sequencing. NoV positivity was observed in 37.5% (39/104) of the surface water samples, with genogroup GI (69.2%) occurring at a higher frequency than GII (25.7%), with a cocirculation of both genogroups in two samples (5.1%). This virus was detected in all sampling points analyzed, showing the highest detection rate at the Paraíso Beach (46.2%). Statistically, there was a dependence relationship between tide levels and positive detection, with a higher frequency at high tide (46.7%) than at low tide (25%) periods. Months with the highest detection rates (April 2012 and April/May 2013) were preceded by periods of higher precipitation (March 2012 and February/March 2013). Phylogenetic analysis showed the circulation of the old pandemic variant (GII.4-US_95-96) and GI.8. The NoV detection demonstrated viral contamination on the beaches and evidenced the health risk to bathers, mainly through recreational activities such as bathing, and highlighted the importance of including enteric viruses research in the recreational water quality monitoring. |
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Deus, Danielle Rodrigues deTeixeira, Dielle MonteiroAlves, Jainara Cristina dos SantosSmith, Vanessa CavaleiroBandeira, Renato da SilvaSiqueira, Jones Anderson MonteiroMoraes, Lena Líllian Canto de SáResque, Hugo ReisGabbay, Yvone Benchimol2019-04-12T16:45:15Z2019-04-12T16:45:15Z2019DEUS, Danielle Rodrigues de et al. Occurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region. Journal of Water & Health, v. 17, n. 3, p. 442-454, 2019. DOI: https://doi.org/10.2166/wh.2019.304. 1477-8920http://patua.iec.gov.br//handle/iec/364210.2166/wh.2019.304This study aimed to investigate the presence of norovirus (NoV) in recreational waters of four estuarine beaches located in Mosqueiro Island, Belém city, Brazilian Amazon, during two years of monitoring (2012 and 2013). NoV particles were concentrated on filtering membrane by the adsorption-elution method and detected by semi-nested RT-PCR and sequencing. NoV positivity was observed in 37.5% (39/104) of the surface water samples, with genogroup GI (69.2%) occurring at a higher frequency than GII (25.7%), with a cocirculation of both genogroups in two samples (5.1%). This virus was detected in all sampling points analyzed, showing the highest detection rate at the Paraíso Beach (46.2%). Statistically, there was a dependence relationship between tide levels and positive detection, with a higher frequency at high tide (46.7%) than at low tide (25%) periods. Months with the highest detection rates (April 2012 and April/May 2013) were preceded by periods of higher precipitation (March 2012 and February/March 2013). Phylogenetic analysis showed the circulation of the old pandemic variant (GII.4-US_95-96) and GI.8. The NoV detection demonstrated viral contamination on the beaches and evidenced the health risk to bathers, mainly through recreational activities such as bathing, and highlighted the importance of including enteric viruses research in the recreational water quality monitoring.State University of Pará. Program in Parasitary Biology in the Amazon. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Programa de Pós-graduação em Virologia. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.engIWA PublishingOccurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon regioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNorovirus / isolamento & purificaçãoNorovirus / virologiaÁgua Doce / virologiaMonitoramento Ambiental / métodosPraiasPoluição das Praias / análiseIlha do Mosqueiro (PA)info:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECLICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/18fba08e-9752-4e12-8244-81fca17eb889/download11832eea31b16df8613079d742d61793MD52TEXTOccurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region.pdf.txtOccurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region.pdf.txtExtracted texttext/plain63352https://patua.iec.gov.br/bitstreams/c757c20f-0add-4392-b172-53a65f924136/downloadb87ac4952bdafc886e9ec1b4ff27dc73MD56THUMBNAILOccurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region.pdf.jpgOccurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region.pdf.jpgGenerated Thumbnailimage/jpeg5177https://patua.iec.gov.br/bitstreams/d0971a7c-abb4-48b5-87c5-1381b75df91c/download97dafa267f85d70a44a53a06ac1faeaeMD57ORIGINALOccurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region.pdfOccurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region.pdfapplication/pdf579778https://patua.iec.gov.br/bitstreams/154d29eb-fb7d-4b59-9b56-18f52ff2ffb8/download81f1ee66f3ed9b824553280e41532cb1MD55iec/36422023-06-13 14:10:30.038oai:patua.iec.gov.br:iec/3642https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2023-06-13T14:10:30Repositório Digital do Instituto Evandro Chagas (Patuá) - 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dc.title.pt_BR.fl_str_mv |
Occurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region |
title |
Occurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region |
spellingShingle |
Occurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region Deus, Danielle Rodrigues de Norovirus / isolamento & purificação Norovirus / virologia Água Doce / virologia Monitoramento Ambiental / métodos Praias Poluição das Praias / análise Ilha do Mosqueiro (PA) |
title_short |
Occurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region |
title_full |
Occurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region |
title_fullStr |
Occurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region |
title_full_unstemmed |
Occurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region |
title_sort |
Occurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region |
author |
Deus, Danielle Rodrigues de |
author_facet |
Deus, Danielle Rodrigues de Teixeira, Dielle Monteiro Alves, Jainara Cristina dos Santos Smith, Vanessa Cavaleiro Bandeira, Renato da Silva Siqueira, Jones Anderson Monteiro Moraes, Lena Líllian Canto de Sá Resque, Hugo Reis Gabbay, Yvone Benchimol |
author_role |
author |
author2 |
Teixeira, Dielle Monteiro Alves, Jainara Cristina dos Santos Smith, Vanessa Cavaleiro Bandeira, Renato da Silva Siqueira, Jones Anderson Monteiro Moraes, Lena Líllian Canto de Sá Resque, Hugo Reis Gabbay, Yvone Benchimol |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Deus, Danielle Rodrigues de Teixeira, Dielle Monteiro Alves, Jainara Cristina dos Santos Smith, Vanessa Cavaleiro Bandeira, Renato da Silva Siqueira, Jones Anderson Monteiro Moraes, Lena Líllian Canto de Sá Resque, Hugo Reis Gabbay, Yvone Benchimol |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Norovirus / isolamento & purificação Norovirus / virologia Água Doce / virologia Monitoramento Ambiental / métodos Praias Poluição das Praias / análise Ilha do Mosqueiro (PA) |
topic |
Norovirus / isolamento & purificação Norovirus / virologia Água Doce / virologia Monitoramento Ambiental / métodos Praias Poluição das Praias / análise Ilha do Mosqueiro (PA) |
description |
This study aimed to investigate the presence of norovirus (NoV) in recreational waters of four estuarine beaches located in Mosqueiro Island, Belém city, Brazilian Amazon, during two years of monitoring (2012 and 2013). NoV particles were concentrated on filtering membrane by the adsorption-elution method and detected by semi-nested RT-PCR and sequencing. NoV positivity was observed in 37.5% (39/104) of the surface water samples, with genogroup GI (69.2%) occurring at a higher frequency than GII (25.7%), with a cocirculation of both genogroups in two samples (5.1%). This virus was detected in all sampling points analyzed, showing the highest detection rate at the Paraíso Beach (46.2%). Statistically, there was a dependence relationship between tide levels and positive detection, with a higher frequency at high tide (46.7%) than at low tide (25%) periods. Months with the highest detection rates (April 2012 and April/May 2013) were preceded by periods of higher precipitation (March 2012 and February/March 2013). Phylogenetic analysis showed the circulation of the old pandemic variant (GII.4-US_95-96) and GI.8. The NoV detection demonstrated viral contamination on the beaches and evidenced the health risk to bathers, mainly through recreational activities such as bathing, and highlighted the importance of including enteric viruses research in the recreational water quality monitoring. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-04-12T16:45:15Z |
dc.date.available.fl_str_mv |
2019-04-12T16:45:15Z |
dc.date.issued.fl_str_mv |
2019 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
DEUS, Danielle Rodrigues de et al. Occurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region. Journal of Water & Health, v. 17, n. 3, p. 442-454, 2019. DOI: https://doi.org/10.2166/wh.2019.304. |
dc.identifier.uri.fl_str_mv |
http://patua.iec.gov.br//handle/iec/3642 |
dc.identifier.issn.-.fl_str_mv |
1477-8920 |
dc.identifier.doi.-.fl_str_mv |
10.2166/wh.2019.304 |
identifier_str_mv |
DEUS, Danielle Rodrigues de et al. Occurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region. Journal of Water & Health, v. 17, n. 3, p. 442-454, 2019. DOI: https://doi.org/10.2166/wh.2019.304. 1477-8920 10.2166/wh.2019.304 |
url |
http://patua.iec.gov.br//handle/iec/3642 |
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eng |
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eng |
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IWA Publishing |
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IWA Publishing |
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