Occurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region

Detalhes bibliográficos
Autor(a) principal: Deus, Danielle Rodrigues de
Data de Publicação: 2019
Outros Autores: Teixeira, Dielle Monteiro, Alves, Jainara Cristina dos Santos, Smith, Vanessa Cavaleiro, Bandeira, Renato da Silva, Siqueira, Jones Anderson Monteiro, Moraes, Lena Líllian Canto de Sá, Resque, Hugo Reis, Gabbay, Yvone Benchimol
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: http://patua.iec.gov.br//handle/iec/3642
Resumo: This study aimed to investigate the presence of norovirus (NoV) in recreational waters of four estuarine beaches located in Mosqueiro Island, Belém city, Brazilian Amazon, during two years of monitoring (2012 and 2013). NoV particles were concentrated on filtering membrane by the adsorption-elution method and detected by semi-nested RT-PCR and sequencing. NoV positivity was observed in 37.5% (39/104) of the surface water samples, with genogroup GI (69.2%) occurring at a higher frequency than GII (25.7%), with a cocirculation of both genogroups in two samples (5.1%). This virus was detected in all sampling points analyzed, showing the highest detection rate at the Paraíso Beach (46.2%). Statistically, there was a dependence relationship between tide levels and positive detection, with a higher frequency at high tide (46.7%) than at low tide (25%) periods. Months with the highest detection rates (April 2012 and April/May 2013) were preceded by periods of higher precipitation (March 2012 and February/March 2013). Phylogenetic analysis showed the circulation of the old pandemic variant (GII.4-US_95-96) and GI.8. The NoV detection demonstrated viral contamination on the beaches and evidenced the health risk to bathers, mainly through recreational activities such as bathing, and highlighted the importance of including enteric viruses research in the recreational water quality monitoring.
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spelling Deus, Danielle Rodrigues deTeixeira, Dielle MonteiroAlves, Jainara Cristina dos SantosSmith, Vanessa CavaleiroBandeira, Renato da SilvaSiqueira, Jones Anderson MonteiroMoraes, Lena Líllian Canto de SáResque, Hugo ReisGabbay, Yvone Benchimol2019-04-12T16:45:15Z2019-04-12T16:45:15Z2019DEUS, Danielle Rodrigues de et al. Occurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region. Journal of Water & Health, v. 17, n. 3, p. 442-454, 2019. DOI: https://doi.org/10.2166/wh.2019.304. 1477-8920http://patua.iec.gov.br//handle/iec/364210.2166/wh.2019.304This study aimed to investigate the presence of norovirus (NoV) in recreational waters of four estuarine beaches located in Mosqueiro Island, Belém city, Brazilian Amazon, during two years of monitoring (2012 and 2013). NoV particles were concentrated on filtering membrane by the adsorption-elution method and detected by semi-nested RT-PCR and sequencing. NoV positivity was observed in 37.5% (39/104) of the surface water samples, with genogroup GI (69.2%) occurring at a higher frequency than GII (25.7%), with a cocirculation of both genogroups in two samples (5.1%). This virus was detected in all sampling points analyzed, showing the highest detection rate at the Paraíso Beach (46.2%). Statistically, there was a dependence relationship between tide levels and positive detection, with a higher frequency at high tide (46.7%) than at low tide (25%) periods. Months with the highest detection rates (April 2012 and April/May 2013) were preceded by periods of higher precipitation (March 2012 and February/March 2013). Phylogenetic analysis showed the circulation of the old pandemic variant (GII.4-US_95-96) and GI.8. The NoV detection demonstrated viral contamination on the beaches and evidenced the health risk to bathers, mainly through recreational activities such as bathing, and highlighted the importance of including enteric viruses research in the recreational water quality monitoring.State University of Pará. Program in Parasitary Biology in the Amazon. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Programa de Pós-graduação em Virologia. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.engIWA PublishingOccurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon regioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNorovirus / isolamento & purificaçãoNorovirus / virologiaÁgua Doce / virologiaMonitoramento Ambiental / métodosPraiasPoluição das Praias / análiseIlha do Mosqueiro (PA)info:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECLICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/18fba08e-9752-4e12-8244-81fca17eb889/download11832eea31b16df8613079d742d61793MD52TEXTOccurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region.pdf.txtOccurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region.pdf.txtExtracted texttext/plain63352https://patua.iec.gov.br/bitstreams/c757c20f-0add-4392-b172-53a65f924136/downloadb87ac4952bdafc886e9ec1b4ff27dc73MD56THUMBNAILOccurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region.pdf.jpgOccurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region.pdf.jpgGenerated Thumbnailimage/jpeg5177https://patua.iec.gov.br/bitstreams/d0971a7c-abb4-48b5-87c5-1381b75df91c/download97dafa267f85d70a44a53a06ac1faeaeMD57ORIGINALOccurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region.pdfOccurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region.pdfapplication/pdf579778https://patua.iec.gov.br/bitstreams/154d29eb-fb7d-4b59-9b56-18f52ff2ffb8/download81f1ee66f3ed9b824553280e41532cb1MD55iec/36422023-06-13 14:10:30.038oai:patua.iec.gov.br:iec/3642https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2023-06-13T14:10:30Repositório Digital do Instituto Evandro Chagas (Patuá) - 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dc.title.pt_BR.fl_str_mv Occurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region
title Occurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region
spellingShingle Occurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region
Deus, Danielle Rodrigues de
Norovirus / isolamento & purificação
Norovirus / virologia
Água Doce / virologia
Monitoramento Ambiental / métodos
Praias
Poluição das Praias / análise
Ilha do Mosqueiro (PA)
title_short Occurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region
title_full Occurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region
title_fullStr Occurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region
title_full_unstemmed Occurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region
title_sort Occurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region
author Deus, Danielle Rodrigues de
author_facet Deus, Danielle Rodrigues de
Teixeira, Dielle Monteiro
Alves, Jainara Cristina dos Santos
Smith, Vanessa Cavaleiro
Bandeira, Renato da Silva
Siqueira, Jones Anderson Monteiro
Moraes, Lena Líllian Canto de Sá
Resque, Hugo Reis
Gabbay, Yvone Benchimol
author_role author
author2 Teixeira, Dielle Monteiro
Alves, Jainara Cristina dos Santos
Smith, Vanessa Cavaleiro
Bandeira, Renato da Silva
Siqueira, Jones Anderson Monteiro
Moraes, Lena Líllian Canto de Sá
Resque, Hugo Reis
Gabbay, Yvone Benchimol
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Deus, Danielle Rodrigues de
Teixeira, Dielle Monteiro
Alves, Jainara Cristina dos Santos
Smith, Vanessa Cavaleiro
Bandeira, Renato da Silva
Siqueira, Jones Anderson Monteiro
Moraes, Lena Líllian Canto de Sá
Resque, Hugo Reis
Gabbay, Yvone Benchimol
dc.subject.decsPrimary.pt_BR.fl_str_mv Norovirus / isolamento & purificação
Norovirus / virologia
Água Doce / virologia
Monitoramento Ambiental / métodos
Praias
Poluição das Praias / análise
Ilha do Mosqueiro (PA)
topic Norovirus / isolamento & purificação
Norovirus / virologia
Água Doce / virologia
Monitoramento Ambiental / métodos
Praias
Poluição das Praias / análise
Ilha do Mosqueiro (PA)
description This study aimed to investigate the presence of norovirus (NoV) in recreational waters of four estuarine beaches located in Mosqueiro Island, Belém city, Brazilian Amazon, during two years of monitoring (2012 and 2013). NoV particles were concentrated on filtering membrane by the adsorption-elution method and detected by semi-nested RT-PCR and sequencing. NoV positivity was observed in 37.5% (39/104) of the surface water samples, with genogroup GI (69.2%) occurring at a higher frequency than GII (25.7%), with a cocirculation of both genogroups in two samples (5.1%). This virus was detected in all sampling points analyzed, showing the highest detection rate at the Paraíso Beach (46.2%). Statistically, there was a dependence relationship between tide levels and positive detection, with a higher frequency at high tide (46.7%) than at low tide (25%) periods. Months with the highest detection rates (April 2012 and April/May 2013) were preceded by periods of higher precipitation (March 2012 and February/March 2013). Phylogenetic analysis showed the circulation of the old pandemic variant (GII.4-US_95-96) and GI.8. The NoV detection demonstrated viral contamination on the beaches and evidenced the health risk to bathers, mainly through recreational activities such as bathing, and highlighted the importance of including enteric viruses research in the recreational water quality monitoring.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-04-12T16:45:15Z
dc.date.available.fl_str_mv 2019-04-12T16:45:15Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv DEUS, Danielle Rodrigues de et al. Occurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region. Journal of Water & Health, v. 17, n. 3, p. 442-454, 2019. DOI: https://doi.org/10.2166/wh.2019.304.
dc.identifier.uri.fl_str_mv http://patua.iec.gov.br//handle/iec/3642
dc.identifier.issn.-.fl_str_mv 1477-8920
dc.identifier.doi.-.fl_str_mv 10.2166/wh.2019.304
identifier_str_mv DEUS, Danielle Rodrigues de et al. Occurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region. Journal of Water & Health, v. 17, n. 3, p. 442-454, 2019. DOI: https://doi.org/10.2166/wh.2019.304.
1477-8920
10.2166/wh.2019.304
url http://patua.iec.gov.br//handle/iec/3642
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collection Repositório Digital do Instituto Evandro Chagas (Patuá)
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