Brazilian Plasmodium falciparum isolates: investigation of candidate polymorphisms for artemisinin resistance before introduction of artemisinin-based combination therapy
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/3559 |
Resumo: | Background: This study was performed to better understand the genetic diversity of known polymorphisms in pfatpase6 and pfmdr1 genes before the introduction of ACT in Brazil, in order to get a genotypic snapshot of Plasmodium falciparum parasites that may be used as baseline reference for future studies. Methods: Parasites from P. falciparum samples collected in 2002, 2004 and 2006-2007 were genotyped using PCR and DNA sequencing at codons 86, 130, 184, 1034, 1042, 1109 and 1246 for pfmdr1 gene, and 243, 263, 402, 431, 623, 630, 639, 683, 716, 776, 769 and 771 for pfatpase6 gene. Results: A pfmdr1 haplotype NEF/CDVY was found in 97% of the samples. In the case of pfatpase6, four haplotypes, wild-type (37%), 630 S (35%), 402 V (5%) and double-mutant 630 S + 402 V (23%), were detected. Conclusion: Although some polymorphism in pfmdr1 and pfatpase6 were verified, no reported haplotypes in both genes that may mediate altered response to ACT was detected before the introduction of this therapy in Brazil. Thus, the haplotypes herein described can be very useful as a baseline reference of P. falciparum populations without ACT drug pressure. |
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Gama, Bianca EOliveira, Natália K Almeida deSouza, José Maria deSantos, FátimaCarvalho, Leonardo José de MouraMelo, Yonne F. CRosenthal, Philip JDaniel-Ribeiro, Cláudio TadeuCruz, Maria de Fátima Ferreira da2019-01-21T11:33:56Z2019-01-21T11:33:56Z2010GAMA, Bianca E. et al. Brazilian Plasmodium falciparum isolates: investigation of candidate polymorphisms for artemisinin resistance before introduction of artemisinin-based combination therapy. Malaria Journal, v. 9, n. 355, p. 1-5, 2010.1475-2875https://patua.iec.gov.br/handle/iec/355910.1186/1475-2875-9-355Background: This study was performed to better understand the genetic diversity of known polymorphisms in pfatpase6 and pfmdr1 genes before the introduction of ACT in Brazil, in order to get a genotypic snapshot of Plasmodium falciparum parasites that may be used as baseline reference for future studies. Methods: Parasites from P. falciparum samples collected in 2002, 2004 and 2006-2007 were genotyped using PCR and DNA sequencing at codons 86, 130, 184, 1034, 1042, 1109 and 1246 for pfmdr1 gene, and 243, 263, 402, 431, 623, 630, 639, 683, 716, 776, 769 and 771 for pfatpase6 gene. Results: A pfmdr1 haplotype NEF/CDVY was found in 97% of the samples. In the case of pfatpase6, four haplotypes, wild-type (37%), 630 S (35%), 402 V (5%) and double-mutant 630 S + 402 V (23%), were detected. Conclusion: Although some polymorphism in pfmdr1 and pfatpase6 were verified, no reported haplotypes in both genes that may mediate altered response to ACT was detected before the introduction of this therapy in Brazil. Thus, the haplotypes herein described can be very useful as a baseline reference of P. falciparum populations without ACT drug pressure.This work was supported by POM/PEF/ Instituto Oswaldo Cruz, PAPES-Fiocruz, and CNPq-PRONEX/DECIT/FAPERJ programs (Brazil).Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratory of Malaria Research. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratory of Malaria Research. Rio de Janeiro, RJ, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ensaios Clínicos Ambulatoriais e Laboratoriais de Malária. Ananindeua, PA, Brasil.Laboratório Central. Laboratory of Entomology. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratory of Malaria Research. Rio de Janeiro, RJ, Brazil / La Jolla Bioengineering Institute. California, USA.Fundação de Medicina Tropical do Amazonas. Manaus, AM, Brazil.University of California. San Francisco General Hospital. Department of Medicine. San Francisco, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratory of Malaria Research. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratory of Malaria Research. Rio de Janeiro, RJ, Brazil.engBMCBrazilian Plasmodium falciparum isolates: investigation of candidate polymorphisms for artemisinin resistance before introduction of artemisinin-based combination therapyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePlasmodium falciparum / genéticaPlasmodium falciparum / isolamento & purificaçãoAdenosina Trifosfatases / genéticaDNA de Protozoário / genéticaResistência a MedicamentosArtemisininas / farmacologiaVariação GenéticaReação em Cadeia da Polimeraseinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALBrazilian Plasmodium falciparum isolates: investigation of candidate polymorphisms for artemisinin resistance before introduction of artemisinin-based combination therapy.pdfBrazilian Plasmodium falciparum isolates: investigation of candidate polymorphisms for artemisinin resistance before introduction of artemisinin-based combination therapy.pdfapplication/pdf253884https://patua.iec.gov.br/bitstreams/0099078b-9f1f-4808-a9ac-82c1337d5bbb/download9d44aa06effb923e521058d742f7b88eMD51LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv |
Brazilian Plasmodium falciparum isolates: investigation of candidate polymorphisms for artemisinin resistance before introduction of artemisinin-based combination therapy |
title |
Brazilian Plasmodium falciparum isolates: investigation of candidate polymorphisms for artemisinin resistance before introduction of artemisinin-based combination therapy |
spellingShingle |
Brazilian Plasmodium falciparum isolates: investigation of candidate polymorphisms for artemisinin resistance before introduction of artemisinin-based combination therapy Gama, Bianca E Plasmodium falciparum / genética Plasmodium falciparum / isolamento & purificação Adenosina Trifosfatases / genética DNA de Protozoário / genética Resistência a Medicamentos Artemisininas / farmacologia Variação Genética Reação em Cadeia da Polimerase |
title_short |
Brazilian Plasmodium falciparum isolates: investigation of candidate polymorphisms for artemisinin resistance before introduction of artemisinin-based combination therapy |
title_full |
Brazilian Plasmodium falciparum isolates: investigation of candidate polymorphisms for artemisinin resistance before introduction of artemisinin-based combination therapy |
title_fullStr |
Brazilian Plasmodium falciparum isolates: investigation of candidate polymorphisms for artemisinin resistance before introduction of artemisinin-based combination therapy |
title_full_unstemmed |
Brazilian Plasmodium falciparum isolates: investigation of candidate polymorphisms for artemisinin resistance before introduction of artemisinin-based combination therapy |
title_sort |
Brazilian Plasmodium falciparum isolates: investigation of candidate polymorphisms for artemisinin resistance before introduction of artemisinin-based combination therapy |
author |
Gama, Bianca E |
author_facet |
Gama, Bianca E Oliveira, Natália K Almeida de Souza, José Maria de Santos, Fátima Carvalho, Leonardo José de Moura Melo, Yonne F. C Rosenthal, Philip J Daniel-Ribeiro, Cláudio Tadeu Cruz, Maria de Fátima Ferreira da |
author_role |
author |
author2 |
Oliveira, Natália K Almeida de Souza, José Maria de Santos, Fátima Carvalho, Leonardo José de Moura Melo, Yonne F. C Rosenthal, Philip J Daniel-Ribeiro, Cláudio Tadeu Cruz, Maria de Fátima Ferreira da |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Gama, Bianca E Oliveira, Natália K Almeida de Souza, José Maria de Santos, Fátima Carvalho, Leonardo José de Moura Melo, Yonne F. C Rosenthal, Philip J Daniel-Ribeiro, Cláudio Tadeu Cruz, Maria de Fátima Ferreira da |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Plasmodium falciparum / genética Plasmodium falciparum / isolamento & purificação Adenosina Trifosfatases / genética DNA de Protozoário / genética Resistência a Medicamentos Artemisininas / farmacologia Variação Genética Reação em Cadeia da Polimerase |
topic |
Plasmodium falciparum / genética Plasmodium falciparum / isolamento & purificação Adenosina Trifosfatases / genética DNA de Protozoário / genética Resistência a Medicamentos Artemisininas / farmacologia Variação Genética Reação em Cadeia da Polimerase |
dc.subject.decsPrimary.none.fl_str_mv |
|
description |
Background: This study was performed to better understand the genetic diversity of known polymorphisms in pfatpase6 and pfmdr1 genes before the introduction of ACT in Brazil, in order to get a genotypic snapshot of Plasmodium falciparum parasites that may be used as baseline reference for future studies. Methods: Parasites from P. falciparum samples collected in 2002, 2004 and 2006-2007 were genotyped using PCR and DNA sequencing at codons 86, 130, 184, 1034, 1042, 1109 and 1246 for pfmdr1 gene, and 243, 263, 402, 431, 623, 630, 639, 683, 716, 776, 769 and 771 for pfatpase6 gene. Results: A pfmdr1 haplotype NEF/CDVY was found in 97% of the samples. In the case of pfatpase6, four haplotypes, wild-type (37%), 630 S (35%), 402 V (5%) and double-mutant 630 S + 402 V (23%), were detected. Conclusion: Although some polymorphism in pfmdr1 and pfatpase6 were verified, no reported haplotypes in both genes that may mediate altered response to ACT was detected before the introduction of this therapy in Brazil. Thus, the haplotypes herein described can be very useful as a baseline reference of P. falciparum populations without ACT drug pressure. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010 |
dc.date.accessioned.fl_str_mv |
2019-01-21T11:33:56Z |
dc.date.available.fl_str_mv |
2019-01-21T11:33:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
GAMA, Bianca E. et al. Brazilian Plasmodium falciparum isolates: investigation of candidate polymorphisms for artemisinin resistance before introduction of artemisinin-based combination therapy. Malaria Journal, v. 9, n. 355, p. 1-5, 2010. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/3559 |
dc.identifier.issn.-.fl_str_mv |
1475-2875 |
dc.identifier.doi.-.fl_str_mv |
10.1186/1475-2875-9-355 |
identifier_str_mv |
GAMA, Bianca E. et al. Brazilian Plasmodium falciparum isolates: investigation of candidate polymorphisms for artemisinin resistance before introduction of artemisinin-based combination therapy. Malaria Journal, v. 9, n. 355, p. 1-5, 2010. 1475-2875 10.1186/1475-2875-9-355 |
url |
https://patua.iec.gov.br/handle/iec/3559 |
dc.language.iso.fl_str_mv |
eng |
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eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
BMC |
publisher.none.fl_str_mv |
BMC |
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Repositório Digital do Instituto Evandro Chagas (Patuá) |
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