An outbreak of group C rotavirus gastroenteritis among children attending a day-care centre in Belém, Brazil

• Autor(a) principal: Gabbay, Yvone Benchimol
• Data de Publicação: 1999
• Outros Autores: Jiang, Baoming, Oliveira, Consuelo Silva de, Mascarenhas, Joana D'Arc Pereira, Leite, José Paulo Gagliardi, Glass, Roger I, Linhares, Alexandre da Costa
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/3284
• topic: Infecções por Rotavirus / complicações; Rotavirus / isolamento & purificação; Rotavirus / patogenicidade; Infecções por Rotavirus / epidemiologia; Serviços Médicos de Emergência / utilização; Gastroenterite / diagnóstico

In August 1993, an outbreak of group C rotavirus-associated gastroenteritis occurred among children attending a day-care centre in Belém, Brazil. Of the 64 children, 21 (33 per cent) became ill. Group C rotavirus was identified in faecal specimens from 8 (38 per cent) children with diarrhoea by electron microscopy (EM) and an enzyme immunoassay (EIA), using antibodies specific to the Cowden strain of porcine group C rotavirus. By polyacrylamide gel electrophoresis (PAGE), a pattern similar to that of group C rotavirus was observed in 5 (62.5 per cent) of the 8 EM- and EIA-positive samples. These 5 faecal samples were confirmed to be positive for group C rotavirus by reverse transcriptase-polymerase chain reaction, using specific VP6 and VP7 primers. This is the first report of an outbreak of diarrhoea in North Brazil associated with group C rotavirus. These findings suggest that group C rotavirus may be an important aetiological agent of diarrhoea in this region, which requires further study