SIPNs polymeric scaffold for use in cartilaginous tissue engineering: physical-chemical evaluation and biological behavior
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/4263 |
Resumo: | Despite the many advances in the field of regenerative medicine, the use of biomaterials to repair the cartilaginous tissue's traumatic injuries is still a challenge. Based on that, this research focuses on the semi-interpenetrating polymeric scaffolds (SIPNs) based PLLA and PHEMA. The material was produced in the solvent absence as a strategy to outline the critical limitations of hydrophilicity and the lack of biological interaction of biomaterials with the surrounding tissues. To this end, the thermal properties and chemical structure were characterized using thermogravimetric analysis and spectroscopy in the Fourier transform infrared. Besides, we assessed porosity and tissue interactions by scanning electron microscopy, and the influence of this in the swelling capacity tests. In vitro and in vivo assays and histological analysis were developed to assess the biocompatibility of SIPNs scaffolds. The results showed good thermal stability, porosity in the range of 53.73 to 94.34 μm, absence of toxicity, and excellent in vitro cell adhesion and growth. The biomaterial also promoted a great template for cell migration and spreading in vivo, with high cartilaginous tissue remodeling. SIPNs scaffolds demonstrated its biocompatibility and potential use in tissue engineering. |
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Dias Junior, Elcio MalcherDias, Dayane dos Reis CostaRodrigues, Ana Paula DrummondDias, Carmen Gilda Barroso TavaresBastos, Gilmara de Nazareth TavaresOliveira, Johnatt Allan Rocha deMaciel Filho, RubensPassos, Marcele Fonseca2021-03-02T16:53:00Z2021-03-02T16:53:00Z2021DIAS JUNIOR, Elcio Malcher et al. SIPNs polymeric scaffold for use in cartilaginous tissue engineering: physical-chemical evaluation and biological behavior. Material Today Communications, v. 26, n. 102111, Mar. 2021.2352-4928https://patua.iec.gov.br/handle/iec/426310.1016/j.mtcomm.2021.102111Despite the many advances in the field of regenerative medicine, the use of biomaterials to repair the cartilaginous tissue's traumatic injuries is still a challenge. Based on that, this research focuses on the semi-interpenetrating polymeric scaffolds (SIPNs) based PLLA and PHEMA. The material was produced in the solvent absence as a strategy to outline the critical limitations of hydrophilicity and the lack of biological interaction of biomaterials with the surrounding tissues. To this end, the thermal properties and chemical structure were characterized using thermogravimetric analysis and spectroscopy in the Fourier transform infrared. Besides, we assessed porosity and tissue interactions by scanning electron microscopy, and the influence of this in the swelling capacity tests. In vitro and in vivo assays and histological analysis were developed to assess the biocompatibility of SIPNs scaffolds. The results showed good thermal stability, porosity in the range of 53.73 to 94.34 μm, absence of toxicity, and excellent in vitro cell adhesion and growth. The biomaterial also promoted a great template for cell migration and spreading in vivo, with high cartilaginous tissue remodeling. SIPNs scaffolds demonstrated its biocompatibility and potential use in tissue engineering.Federal University of Pará. Institute of Biological Sciences. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Microscopia. Ananindeua, PA, Brasil.Federal University of Pará. Department of Mechanical Engineering. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Belém, PA, Brazil.Federal University of Pará. Department of Nutrition. Belém, PA, Brazil.Campinas State University. National Institute of Science and Technology in Biofabrication. Chemical Engineering Department. Campinas, SP, Brazil.Federal University of Pará. Institute of Biological Sciences. Belém, PA, Brazil / Campinas State University. National Institute of Science and Technology in Biofabrication. Chemical Engineering Department. Campinas, SP, Brazil.engElsevierSIPNs polymeric scaffold for use in cartilaginous tissue engineering: physical-chemical evaluation and biological behaviorinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCartilagem / ultraestruturaMateriais BiocompatíveisMedicina RegenerativaPoli-Hidroxietil MetacrilatoEngenharia Tecidualinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALSIPNs polymeric scaffold for use in cartilaginous tissue engineering: physical-chemical evaluation and biological behavior.pdfSIPNs polymeric scaffold for use in cartilaginous tissue engineering: physical-chemical evaluation and biological behavior.pdfapplication/pdf551083https://patua.iec.gov.br/bitstreams/ba937344-04fc-4841-ab6f-439de34aee64/downloadc9a9c128e29cac82a5d7fdf3f4e6da73MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/88ee57e9-ff53-451a-b0c6-6aa8bafa2c96/download11832eea31b16df8613079d742d61793MD52TEXTSIPNs polymeric scaffold for use in cartilaginous tissue engineering: physical-chemical evaluation and biological behavior.pdf.txtSIPNs polymeric scaffold for use in cartilaginous tissue engineering: physical-chemical evaluation and biological behavior.pdf.txtExtracted texttext/plain2https://patua.iec.gov.br/bitstreams/39286094-bf04-4381-b54b-402107565cba/downloade1c06d85ae7b8b032bef47e42e4c08f9MD55THUMBNAILSIPNs polymeric scaffold for use in cartilaginous tissue engineering: physical-chemical evaluation and biological behavior.pdf.jpgSIPNs polymeric scaffold for use in cartilaginous tissue engineering: physical-chemical evaluation and biological behavior.pdf.jpgGenerated Thumbnailimage/jpeg3095https://patua.iec.gov.br/bitstreams/36cba2b5-e26b-4afb-8ed2-9f47e3a824e8/download71859d578212107f7f8c49a4ce09d9eeMD56iec/42632022-10-20 22:55:02.245oai:patua.iec.gov.br:iec/4263https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T22:55:02Repositório Digital do Instituto Evandro Chagas (Patuá) - 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dc.title.pt_BR.fl_str_mv |
SIPNs polymeric scaffold for use in cartilaginous tissue engineering: physical-chemical evaluation and biological behavior |
title |
SIPNs polymeric scaffold for use in cartilaginous tissue engineering: physical-chemical evaluation and biological behavior |
spellingShingle |
SIPNs polymeric scaffold for use in cartilaginous tissue engineering: physical-chemical evaluation and biological behavior Dias Junior, Elcio Malcher Cartilagem / ultraestrutura Materiais Biocompatíveis Medicina Regenerativa Poli-Hidroxietil Metacrilato Engenharia Tecidual |
title_short |
SIPNs polymeric scaffold for use in cartilaginous tissue engineering: physical-chemical evaluation and biological behavior |
title_full |
SIPNs polymeric scaffold for use in cartilaginous tissue engineering: physical-chemical evaluation and biological behavior |
title_fullStr |
SIPNs polymeric scaffold for use in cartilaginous tissue engineering: physical-chemical evaluation and biological behavior |
title_full_unstemmed |
SIPNs polymeric scaffold for use in cartilaginous tissue engineering: physical-chemical evaluation and biological behavior |
title_sort |
SIPNs polymeric scaffold for use in cartilaginous tissue engineering: physical-chemical evaluation and biological behavior |
author |
Dias Junior, Elcio Malcher |
author_facet |
Dias Junior, Elcio Malcher Dias, Dayane dos Reis Costa Rodrigues, Ana Paula Drummond Dias, Carmen Gilda Barroso Tavares Bastos, Gilmara de Nazareth Tavares Oliveira, Johnatt Allan Rocha de Maciel Filho, Rubens Passos, Marcele Fonseca |
author_role |
author |
author2 |
Dias, Dayane dos Reis Costa Rodrigues, Ana Paula Drummond Dias, Carmen Gilda Barroso Tavares Bastos, Gilmara de Nazareth Tavares Oliveira, Johnatt Allan Rocha de Maciel Filho, Rubens Passos, Marcele Fonseca |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Dias Junior, Elcio Malcher Dias, Dayane dos Reis Costa Rodrigues, Ana Paula Drummond Dias, Carmen Gilda Barroso Tavares Bastos, Gilmara de Nazareth Tavares Oliveira, Johnatt Allan Rocha de Maciel Filho, Rubens Passos, Marcele Fonseca |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Cartilagem / ultraestrutura Materiais Biocompatíveis Medicina Regenerativa Poli-Hidroxietil Metacrilato Engenharia Tecidual |
topic |
Cartilagem / ultraestrutura Materiais Biocompatíveis Medicina Regenerativa Poli-Hidroxietil Metacrilato Engenharia Tecidual |
description |
Despite the many advances in the field of regenerative medicine, the use of biomaterials to repair the cartilaginous tissue's traumatic injuries is still a challenge. Based on that, this research focuses on the semi-interpenetrating polymeric scaffolds (SIPNs) based PLLA and PHEMA. The material was produced in the solvent absence as a strategy to outline the critical limitations of hydrophilicity and the lack of biological interaction of biomaterials with the surrounding tissues. To this end, the thermal properties and chemical structure were characterized using thermogravimetric analysis and spectroscopy in the Fourier transform infrared. Besides, we assessed porosity and tissue interactions by scanning electron microscopy, and the influence of this in the swelling capacity tests. In vitro and in vivo assays and histological analysis were developed to assess the biocompatibility of SIPNs scaffolds. The results showed good thermal stability, porosity in the range of 53.73 to 94.34 μm, absence of toxicity, and excellent in vitro cell adhesion and growth. The biomaterial also promoted a great template for cell migration and spreading in vivo, with high cartilaginous tissue remodeling. SIPNs scaffolds demonstrated its biocompatibility and potential use in tissue engineering. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-03-02T16:53:00Z |
dc.date.available.fl_str_mv |
2021-03-02T16:53:00Z |
dc.date.issued.fl_str_mv |
2021 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
DIAS JUNIOR, Elcio Malcher et al. SIPNs polymeric scaffold for use in cartilaginous tissue engineering: physical-chemical evaluation and biological behavior. Material Today Communications, v. 26, n. 102111, Mar. 2021. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/4263 |
dc.identifier.issn.-.fl_str_mv |
2352-4928 |
dc.identifier.doi.-.fl_str_mv |
10.1016/j.mtcomm.2021.102111 |
identifier_str_mv |
DIAS JUNIOR, Elcio Malcher et al. SIPNs polymeric scaffold for use in cartilaginous tissue engineering: physical-chemical evaluation and biological behavior. Material Today Communications, v. 26, n. 102111, Mar. 2021. 2352-4928 10.1016/j.mtcomm.2021.102111 |
url |
https://patua.iec.gov.br/handle/iec/4263 |
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eng |
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eng |
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info:eu-repo/semantics/embargoedAccess |
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Elsevier |
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Elsevier |
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Instituto Evandro Chagas (IEC) |
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IEC |
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IEC |
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Repositório Digital do Instituto Evandro Chagas (Patuá) |
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Repositório Digital do Instituto Evandro Chagas (Patuá) |
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