Molecular epidemiology of human bocavirus in children with acute gastroenteritis from North Region of Brazil

Detalhes bibliográficos
Autor(a) principal: Soares, Luana da Silva
Data de Publicação: 2019
Outros Autores: Lima, Ana Beatriz F, Pantoja, Kamilla C, Lobo, Patrícia dos Santos, Cruz, Jonas F, Guerra, Sylvia de Fátima dos Santos, Bezerra, Delana Andreza Melo, Bandeira, Renato S, Mascarenhas, Joana D'Arc Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/3852
Resumo: PURPOSE: Human bocavirus (HBoV) is a DNA virus that is mostly associated with respiratory infections. However, because it has been found in stool samples, it has been suggested that it may be a causative agent for human enteric conditions. This underpins the continuous search for HBoVs, especially after the introduction of the rotavirus vaccine due to acute gastroenteritis cases related to emergent viruses, as HBoVs are more likely to be found in this post-vaccine scenario. Therefore, the aim of this study is to demonstrate the prevalence of HBoV in children aged less than 10 years with acute gastroenteritis in Brazil from November 2011 to November 2012. METHODOLOGY: Stool samples from hospitalized children ≤10 years old who presented symptoms of acute gastroenteritis were analysed for the presence of rotavirus A (RVA) by an enzyme-linked immunosorbent assay (ELISA), and for HBoV DNA by nested PCR. RESULTS: HBoV positivity was detected in 24.0 % (54/225) of samples. Two peaks of HBoV detection were observed in November 2011 and from July to September 2012. Co-infections between HBoV and rotavirus A were identified in 50.0 % (27/54) of specimens. Phylogenetic analysis identified the presence of HBoV-1 (94.8 %), HBoV-2 (2.6 %) and HBoV-3 (2.6 %) species, with only minor variations among them. CONCLUSION: Our findings provide evidence for the circulation of most HBoV genotypes (except HBoV-4) in the North Region of Brazil at a considerable rate and further investigations are necessary to improve our knowledge in the context of HBoV infections and their role in gastrointestinal diseases.
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spelling Soares, Luana da SilvaLima, Ana Beatriz FPantoja, Kamilla CLobo, Patrícia dos SantosCruz, Jonas FGuerra, Sylvia de Fátima dos SantosBezerra, Delana Andreza MeloBandeira, Renato SMascarenhas, Joana D'Arc Pereira2019-08-21T17:57:01Z2019-08-21T17:57:01Z2019SOARES, Luana da Silva et al. Molecular epidemiology of human bocavirus in children with acute gastroenteritis from North Region of Brazil. Journal of Medical Microbiology, v. 68, n. 8, p. 1233-1239, Aug. 2019. DOI: https://doi.org/ 10.1099/jmm.0.001026. Disponível em: https://www.microbiologyresearch.org/docserver/fulltext/jmm/68/8/1233_jmm001026.pdfexpires=1671043406&id=id&accname=guest&checksum=67347576F825EFD6D50AC0E1649A02A5.0022-2615https://patua.iec.gov.br/handle/iec/385210.1099/jmm.0.001026PURPOSE: Human bocavirus (HBoV) is a DNA virus that is mostly associated with respiratory infections. However, because it has been found in stool samples, it has been suggested that it may be a causative agent for human enteric conditions. This underpins the continuous search for HBoVs, especially after the introduction of the rotavirus vaccine due to acute gastroenteritis cases related to emergent viruses, as HBoVs are more likely to be found in this post-vaccine scenario. Therefore, the aim of this study is to demonstrate the prevalence of HBoV in children aged less than 10 years with acute gastroenteritis in Brazil from November 2011 to November 2012. METHODOLOGY: Stool samples from hospitalized children ≤10 years old who presented symptoms of acute gastroenteritis were analysed for the presence of rotavirus A (RVA) by an enzyme-linked immunosorbent assay (ELISA), and for HBoV DNA by nested PCR. RESULTS: HBoV positivity was detected in 24.0 % (54/225) of samples. Two peaks of HBoV detection were observed in November 2011 and from July to September 2012. Co-infections between HBoV and rotavirus A were identified in 50.0 % (27/54) of specimens. Phylogenetic analysis identified the presence of HBoV-1 (94.8 %), HBoV-2 (2.6 %) and HBoV-3 (2.6 %) species, with only minor variations among them. CONCLUSION: Our findings provide evidence for the circulation of most HBoV genotypes (except HBoV-4) in the North Region of Brazil at a considerable rate and further investigations are necessary to improve our knowledge in the context of HBoV infections and their role in gastrointestinal diseases.National Council for Scientific and Technological Development (CNPq)Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.engMicrobiology SocietyMolecular epidemiology of human bocavirus in children with acute gastroenteritis from North Region of Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBocavirus Humano / patogenicidadeGastroenterite / virologiaFezes / virologiaEnsaio de Imunoadsorção Enzimática / métodosReação em Cadeia da Polimerase / métodosPrevalênciainfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECLICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/ec72c207-abeb-4ea7-9113-837535dbe326/download11832eea31b16df8613079d742d61793MD52TEXTMolecular epidemiology of human bocavirus in children with acute gastroenteritis from North Region of Brazil.pdf.txtMolecular epidemiology of human bocavirus in children with acute gastroenteritis from North Region of Brazil.pdf.txtExtracted texttext/plain25837https://patua.iec.gov.br/bitstreams/92191df3-208a-41c6-8113-2128d9507037/download418abda106c2875ac5a22c6bab097b15MD56THUMBNAILMolecular epidemiology of human bocavirus in children with acute gastroenteritis from North Region of Brazil.pdf.jpgMolecular epidemiology of human bocavirus in children with acute gastroenteritis from North Region of Brazil.pdf.jpgGenerated Thumbnailimage/jpeg5919https://patua.iec.gov.br/bitstreams/25b824e3-84e9-446f-aa9c-28c3dd12a79f/downloadbbc01af27768a3510c30486397dea8b3MD57ORIGINALMolecular epidemiology of human bocavirus in children with acute gastroenteritis from North Region of Brazil.pdfMolecular epidemiology of human bocavirus in children with acute gastroenteritis from North Region of Brazil.pdfapplication/pdf1723125https://patua.iec.gov.br/bitstreams/0874fd3d-f505-4a7b-9840-84d3d0cf84cd/download1461105ad5c6b52b8e6d1fa335918aa7MD55iec/38522022-12-14 18:29:40.447oai:patua.iec.gov.br:iec/3852https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-12-14T18:29:40Repositório Digital do Instituto Evandro Chagas (Patuá) - 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dc.title.pt_BR.fl_str_mv Molecular epidemiology of human bocavirus in children with acute gastroenteritis from North Region of Brazil
title Molecular epidemiology of human bocavirus in children with acute gastroenteritis from North Region of Brazil
spellingShingle Molecular epidemiology of human bocavirus in children with acute gastroenteritis from North Region of Brazil
Soares, Luana da Silva
Bocavirus Humano / patogenicidade
Gastroenterite / virologia
Fezes / virologia
Ensaio de Imunoadsorção Enzimática / métodos
Reação em Cadeia da Polimerase / métodos
Prevalência
title_short Molecular epidemiology of human bocavirus in children with acute gastroenteritis from North Region of Brazil
title_full Molecular epidemiology of human bocavirus in children with acute gastroenteritis from North Region of Brazil
title_fullStr Molecular epidemiology of human bocavirus in children with acute gastroenteritis from North Region of Brazil
title_full_unstemmed Molecular epidemiology of human bocavirus in children with acute gastroenteritis from North Region of Brazil
title_sort Molecular epidemiology of human bocavirus in children with acute gastroenteritis from North Region of Brazil
author Soares, Luana da Silva
author_facet Soares, Luana da Silva
Lima, Ana Beatriz F
Pantoja, Kamilla C
Lobo, Patrícia dos Santos
Cruz, Jonas F
Guerra, Sylvia de Fátima dos Santos
Bezerra, Delana Andreza Melo
Bandeira, Renato S
Mascarenhas, Joana D'Arc Pereira
author_role author
author2 Lima, Ana Beatriz F
Pantoja, Kamilla C
Lobo, Patrícia dos Santos
Cruz, Jonas F
Guerra, Sylvia de Fátima dos Santos
Bezerra, Delana Andreza Melo
Bandeira, Renato S
Mascarenhas, Joana D'Arc Pereira
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Soares, Luana da Silva
Lima, Ana Beatriz F
Pantoja, Kamilla C
Lobo, Patrícia dos Santos
Cruz, Jonas F
Guerra, Sylvia de Fátima dos Santos
Bezerra, Delana Andreza Melo
Bandeira, Renato S
Mascarenhas, Joana D'Arc Pereira
dc.subject.decsPrimary.pt_BR.fl_str_mv Bocavirus Humano / patogenicidade
Gastroenterite / virologia
Fezes / virologia
Ensaio de Imunoadsorção Enzimática / métodos
Reação em Cadeia da Polimerase / métodos
Prevalência
topic Bocavirus Humano / patogenicidade
Gastroenterite / virologia
Fezes / virologia
Ensaio de Imunoadsorção Enzimática / métodos
Reação em Cadeia da Polimerase / métodos
Prevalência
description PURPOSE: Human bocavirus (HBoV) is a DNA virus that is mostly associated with respiratory infections. However, because it has been found in stool samples, it has been suggested that it may be a causative agent for human enteric conditions. This underpins the continuous search for HBoVs, especially after the introduction of the rotavirus vaccine due to acute gastroenteritis cases related to emergent viruses, as HBoVs are more likely to be found in this post-vaccine scenario. Therefore, the aim of this study is to demonstrate the prevalence of HBoV in children aged less than 10 years with acute gastroenteritis in Brazil from November 2011 to November 2012. METHODOLOGY: Stool samples from hospitalized children ≤10 years old who presented symptoms of acute gastroenteritis were analysed for the presence of rotavirus A (RVA) by an enzyme-linked immunosorbent assay (ELISA), and for HBoV DNA by nested PCR. RESULTS: HBoV positivity was detected in 24.0 % (54/225) of samples. Two peaks of HBoV detection were observed in November 2011 and from July to September 2012. Co-infections between HBoV and rotavirus A were identified in 50.0 % (27/54) of specimens. Phylogenetic analysis identified the presence of HBoV-1 (94.8 %), HBoV-2 (2.6 %) and HBoV-3 (2.6 %) species, with only minor variations among them. CONCLUSION: Our findings provide evidence for the circulation of most HBoV genotypes (except HBoV-4) in the North Region of Brazil at a considerable rate and further investigations are necessary to improve our knowledge in the context of HBoV infections and their role in gastrointestinal diseases.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-08-21T17:57:01Z
dc.date.available.fl_str_mv 2019-08-21T17:57:01Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.citation.fl_str_mv SOARES, Luana da Silva et al. Molecular epidemiology of human bocavirus in children with acute gastroenteritis from North Region of Brazil. Journal of Medical Microbiology, v. 68, n. 8, p. 1233-1239, Aug. 2019. DOI: https://doi.org/ 10.1099/jmm.0.001026. Disponível em: https://www.microbiologyresearch.org/docserver/fulltext/jmm/68/8/1233_jmm001026.pdfexpires=1671043406&id=id&accname=guest&checksum=67347576F825EFD6D50AC0E1649A02A5.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/3852
dc.identifier.issn.-.fl_str_mv 0022-2615
dc.identifier.doi.-.fl_str_mv 10.1099/jmm.0.001026
identifier_str_mv SOARES, Luana da Silva et al. Molecular epidemiology of human bocavirus in children with acute gastroenteritis from North Region of Brazil. Journal of Medical Microbiology, v. 68, n. 8, p. 1233-1239, Aug. 2019. DOI: https://doi.org/ 10.1099/jmm.0.001026. Disponível em: https://www.microbiologyresearch.org/docserver/fulltext/jmm/68/8/1233_jmm001026.pdfexpires=1671043406&id=id&accname=guest&checksum=67347576F825EFD6D50AC0E1649A02A5.
0022-2615
10.1099/jmm.0.001026
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