High prevalence of G12P[8] rotavirus strains in Rio Branco, Acre, Western Amazon, in the post-rotavirus vaccine introduction period
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | http://patua.iec.gov.br/handle/iec/2429 |
Resumo: | The present study aimed to provide a molecular characterization of circulating rotavirus (RVA) strains in Rio Branco, Acre, in the post-rotavirus vaccination period, particularly with regard to the emerging, increasingly prevalent G12P[8] genotype. A total of 488 fecal specimens from diarrheic and non-diarrheic children were obtained between January and December 2012. RVA detection was initially performed using enzyme-linked immunosorbent assay (ELISA) method, followed by reverse-transcription polymerase chain reaction (RT-PCR) using specific primers. RVA was detected in 18.3 per cent (44/241) of the children with acute diarrhea and in 1.2 per cent (3/247) of the non-diarrheic children (P  0.001), with overall RVA-positivity of 9.6 per cent (47/488). The most common genotype was G2P[4] with 43.2 per cent (19/44) of the diarrheic cases, followed by G12P[8] (27.3 per cent, 12/44), G3P[6] (18.2 per cent, 8/44), G3P[8] (4.5 per cent, 2/44), and G12P[6] (2.3 per cent, 1/44). G12 samples belonged to lineage III and were from children aged 4û52 months. All of these children had acute diarrhea associated with fever (83.3 per cent, 10/12) and vomiting (66.7 per cent, 8/12). Most of the cases occurred in August (58.3 per cent, 7/12), 75 per cent (9/12) of which having received the full vaccination scheme with RotarixÖ. For the first time G12 was reported at relative high prevalence in Brazil. Our findings warrant further monitoring studies on the molecular characterization of circulating RVA strains after rotavirus vaccine introduction in Brazil and elsewhere, since the occurrence of either unusual our emerging genotypes may pose a challenge to vaccination strategies. |
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Neves, Mayara A. OPinheiro, Helder H. CSilva, Rita do Socorro Uchôa daLinhares, Alexandre da CostaSilva, Luciana Damascena daGabbay, Yvone BenchimolSilva, Mônica Cristina de MoraesLoureiro, Edvaldo Carlos BritoSoares, Luana da SilvaMascarenhas, Joana D'Arc Pereira2017-03-24T17:47:24Z2017-03-24T17:47:24Z2016NEVES, Mayara A. O. et al. High prevalence of G12P[8] rotavirus strains in Rio Branco, Acre, Western Amazon, in the post-rotavirus vaccine introduction period. Journal of Medical Virology, v. 88, n. 5, p. 782-789, May 2016. DOI: https://doi.org/10.1002/jmv.244041096-9071http://patua.iec.gov.br/handle/iec/242910.1002/jmv.24404The present study aimed to provide a molecular characterization of circulating rotavirus (RVA) strains in Rio Branco, Acre, in the post-rotavirus vaccination period, particularly with regard to the emerging, increasingly prevalent G12P[8] genotype. A total of 488 fecal specimens from diarrheic and non-diarrheic children were obtained between January and December 2012. RVA detection was initially performed using enzyme-linked immunosorbent assay (ELISA) method, followed by reverse-transcription polymerase chain reaction (RT-PCR) using specific primers. RVA was detected in 18.3 per cent (44/241) of the children with acute diarrhea and in 1.2 per cent (3/247) of the non-diarrheic children (P  0.001), with overall RVA-positivity of 9.6 per cent (47/488). The most common genotype was G2P[4] with 43.2 per cent (19/44) of the diarrheic cases, followed by G12P[8] (27.3 per cent, 12/44), G3P[6] (18.2 per cent, 8/44), G3P[8] (4.5 per cent, 2/44), and G12P[6] (2.3 per cent, 1/44). G12 samples belonged to lineage III and were from children aged 4û52 months. All of these children had acute diarrhea associated with fever (83.3 per cent, 10/12) and vomiting (66.7 per cent, 8/12). Most of the cases occurred in August (58.3 per cent, 7/12), 75 per cent (9/12) of which having received the full vaccination scheme with RotarixÖ. For the first time G12 was reported at relative high prevalence in Brazil. Our findings warrant further monitoring studies on the molecular characterization of circulating RVA strains after rotavirus vaccine introduction in Brazil and elsewhere, since the occurrence of either unusual our emerging genotypes may pose a challenge to vaccination strategies.University of State of Pará. Center for Biological and Health Sciences. Belém, PA, Brazil.Federal University of Pará, Pará. Center for Tropical Medicine. Belém, PA, Brazil.Municipality Secretary of Health. Rio Branco, Acre, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.application/pdfengWileyHigh prevalence of G12P[8] rotavirus strains in Rio Branco, Acre, Western Amazon, in the post-rotavirus vaccine introduction periodinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRotavirus / classificaçãoRotavirus / genéticaInfecções por Rotavirus / epidemiologiaInfecções por Rotavirus / virologiaDiarreia InfantilGenótipoEnsaio de Imunoadsorção Enzimática / métodosReação em Cadeia da Polimerase Via Transcriptase Reversa / métodosinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECTHUMBNAILHigh prevalence of G12P[8] rotavirus strains in Rio Branco, Acre, Western Amazon, in the post-rotavirus vaccine introduction period.pdf.jpgHigh prevalence of G12P[8] rotavirus strains in Rio Branco, Acre, Western Amazon, in the post-rotavirus vaccine introduction period.pdf.jpgGenerated Thumbnailimage/jpeg3095https://patua.iec.gov.br/bitstreams/f9e20ba2-baba-424c-8742-ecf6b535fb04/download71859d578212107f7f8c49a4ce09d9eeMD57Acesso Embargado.pdf.jpgAcesso Embargado.pdf.jpgGenerated Thumbnailimage/jpeg3095https://patua.iec.gov.br/bitstreams/9983822b-6165-4abe-b27c-be26f2bdef7a/download71859d578212107f7f8c49a4ce09d9eeMD59ORIGINALAcesso Embargado.pdfAcesso Embargado.pdfapplication/pdf551083https://patua.iec.gov.br/bitstreams/a1039ea5-247e-46ff-b00e-fb90d8169741/downloadc9a9c128e29cac82a5d7fdf3f4e6da73MD54TEXTHigh prevalence of G12P[8] rotavirus strains in Rio Branco, Acre, Western Amazon, in the post-rotavirus vaccine introduction period.pdf.txtHigh prevalence of G12P[8] rotavirus strains in Rio Branco, Acre, Western Amazon, in the post-rotavirus vaccine introduction period.pdf.txtExtracted texttext/plain2https://patua.iec.gov.br/bitstreams/6d2c2acd-f3e9-4576-bc6c-8114e0fe3af9/downloade1c06d85ae7b8b032bef47e42e4c08f9MD56Acesso Embargado.pdf.txtAcesso Embargado.pdf.txtExtracted texttext/plain2https://patua.iec.gov.br/bitstreams/365ff7fa-927e-48b9-83ba-349eecbac7ef/downloade1c06d85ae7b8b032bef47e42e4c08f9MD58LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv |
High prevalence of G12P[8] rotavirus strains in Rio Branco, Acre, Western Amazon, in the post-rotavirus vaccine introduction period |
title |
High prevalence of G12P[8] rotavirus strains in Rio Branco, Acre, Western Amazon, in the post-rotavirus vaccine introduction period |
spellingShingle |
High prevalence of G12P[8] rotavirus strains in Rio Branco, Acre, Western Amazon, in the post-rotavirus vaccine introduction period Neves, Mayara A. O Rotavirus / classificação Rotavirus / genética Infecções por Rotavirus / epidemiologia Infecções por Rotavirus / virologia Diarreia Infantil Genótipo Ensaio de Imunoadsorção Enzimática / métodos Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos |
title_short |
High prevalence of G12P[8] rotavirus strains in Rio Branco, Acre, Western Amazon, in the post-rotavirus vaccine introduction period |
title_full |
High prevalence of G12P[8] rotavirus strains in Rio Branco, Acre, Western Amazon, in the post-rotavirus vaccine introduction period |
title_fullStr |
High prevalence of G12P[8] rotavirus strains in Rio Branco, Acre, Western Amazon, in the post-rotavirus vaccine introduction period |
title_full_unstemmed |
High prevalence of G12P[8] rotavirus strains in Rio Branco, Acre, Western Amazon, in the post-rotavirus vaccine introduction period |
title_sort |
High prevalence of G12P[8] rotavirus strains in Rio Branco, Acre, Western Amazon, in the post-rotavirus vaccine introduction period |
author |
Neves, Mayara A. O |
author_facet |
Neves, Mayara A. O Pinheiro, Helder H. C Silva, Rita do Socorro Uchôa da Linhares, Alexandre da Costa Silva, Luciana Damascena da Gabbay, Yvone Benchimol Silva, Mônica Cristina de Moraes Loureiro, Edvaldo Carlos Brito Soares, Luana da Silva Mascarenhas, Joana D'Arc Pereira |
author_role |
author |
author2 |
Pinheiro, Helder H. C Silva, Rita do Socorro Uchôa da Linhares, Alexandre da Costa Silva, Luciana Damascena da Gabbay, Yvone Benchimol Silva, Mônica Cristina de Moraes Loureiro, Edvaldo Carlos Brito Soares, Luana da Silva Mascarenhas, Joana D'Arc Pereira |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Neves, Mayara A. O Pinheiro, Helder H. C Silva, Rita do Socorro Uchôa da Linhares, Alexandre da Costa Silva, Luciana Damascena da Gabbay, Yvone Benchimol Silva, Mônica Cristina de Moraes Loureiro, Edvaldo Carlos Brito Soares, Luana da Silva Mascarenhas, Joana D'Arc Pereira |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Rotavirus / classificação Rotavirus / genética Infecções por Rotavirus / epidemiologia Infecções por Rotavirus / virologia Diarreia Infantil Genótipo Ensaio de Imunoadsorção Enzimática / métodos Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos |
topic |
Rotavirus / classificação Rotavirus / genética Infecções por Rotavirus / epidemiologia Infecções por Rotavirus / virologia Diarreia Infantil Genótipo Ensaio de Imunoadsorção Enzimática / métodos Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos |
description |
The present study aimed to provide a molecular characterization of circulating rotavirus (RVA) strains in Rio Branco, Acre, in the post-rotavirus vaccination period, particularly with regard to the emerging, increasingly prevalent G12P[8] genotype. A total of 488 fecal specimens from diarrheic and non-diarrheic children were obtained between January and December 2012. RVA detection was initially performed using enzyme-linked immunosorbent assay (ELISA) method, followed by reverse-transcription polymerase chain reaction (RT-PCR) using specific primers. RVA was detected in 18.3 per cent (44/241) of the children with acute diarrhea and in 1.2 per cent (3/247) of the non-diarrheic children (P  0.001), with overall RVA-positivity of 9.6 per cent (47/488). The most common genotype was G2P[4] with 43.2 per cent (19/44) of the diarrheic cases, followed by G12P[8] (27.3 per cent, 12/44), G3P[6] (18.2 per cent, 8/44), G3P[8] (4.5 per cent, 2/44), and G12P[6] (2.3 per cent, 1/44). G12 samples belonged to lineage III and were from children aged 4û52 months. All of these children had acute diarrhea associated with fever (83.3 per cent, 10/12) and vomiting (66.7 per cent, 8/12). Most of the cases occurred in August (58.3 per cent, 7/12), 75 per cent (9/12) of which having received the full vaccination scheme with RotarixÖ. For the first time G12 was reported at relative high prevalence in Brazil. Our findings warrant further monitoring studies on the molecular characterization of circulating RVA strains after rotavirus vaccine introduction in Brazil and elsewhere, since the occurrence of either unusual our emerging genotypes may pose a challenge to vaccination strategies. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
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2017-03-24T17:47:24Z |
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2017-03-24T17:47:24Z |
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dc.identifier.citation.fl_str_mv |
NEVES, Mayara A. O. et al. High prevalence of G12P[8] rotavirus strains in Rio Branco, Acre, Western Amazon, in the post-rotavirus vaccine introduction period. Journal of Medical Virology, v. 88, n. 5, p. 782-789, May 2016. DOI: https://doi.org/10.1002/jmv.24404 |
dc.identifier.uri.fl_str_mv |
http://patua.iec.gov.br/handle/iec/2429 |
dc.identifier.issn.-.fl_str_mv |
1096-9071 |
dc.identifier.doi.-.fl_str_mv |
10.1002/jmv.24404 |
identifier_str_mv |
NEVES, Mayara A. O. et al. High prevalence of G12P[8] rotavirus strains in Rio Branco, Acre, Western Amazon, in the post-rotavirus vaccine introduction period. Journal of Medical Virology, v. 88, n. 5, p. 782-789, May 2016. DOI: https://doi.org/10.1002/jmv.24404 1096-9071 10.1002/jmv.24404 |
url |
http://patua.iec.gov.br/handle/iec/2429 |
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Wiley |
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