High prevalence of G12P[8] rotavirus strains in Rio Branco, Acre, Western Amazon, in the post-rotavirus vaccine introduction period

Detalhes bibliográficos
Autor(a) principal: Neves, Mayara A. O
Data de Publicação: 2016
Outros Autores: Pinheiro, Helder H. C, Silva, Rita do Socorro Uchôa da, Linhares, Alexandre da Costa, Silva, Luciana Damascena da, Gabbay, Yvone Benchimol, Silva, Mônica Cristina de Moraes, Loureiro, Edvaldo Carlos Brito, Soares, Luana da Silva, Mascarenhas, Joana D'Arc Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: http://patua.iec.gov.br/handle/iec/2429
Resumo: The present study aimed to provide a molecular characterization of circulating rotavirus (RVA) strains in Rio Branco, Acre, in the post-rotavirus vaccination period, particularly with regard to the emerging, increasingly prevalent G12P[8] genotype. A total of 488 fecal specimens from diarrheic and non-diarrheic children were obtained between January and December 2012. RVA detection was initially performed using enzyme-linked immunosorbent assay (ELISA) method, followed by reverse-transcription polymerase chain reaction (RT-PCR) using specific primers. RVA was detected in 18.3 per cent (44/241) of the children with acute diarrhea and in 1.2 per cent (3/247) of the non-diarrheic children (P  0.001), with overall RVA-positivity of 9.6 per cent (47/488). The most common genotype was G2P[4] with 43.2 per cent (19/44) of the diarrheic cases, followed by G12P[8] (27.3 per cent, 12/44), G3P[6] (18.2 per cent, 8/44), G3P[8] (4.5 per cent, 2/44), and G12P[6] (2.3 per cent, 1/44). G12 samples belonged to lineage III and were from children aged 4û52 months. All of these children had acute diarrhea associated with fever (83.3 per cent, 10/12) and vomiting (66.7 per cent, 8/12). Most of the cases occurred in August (58.3 per cent, 7/12), 75 per cent (9/12) of which having received the full vaccination scheme with RotarixÖ. For the first time G12 was reported at relative high prevalence in Brazil. Our findings warrant further monitoring studies on the molecular characterization of circulating RVA strains after rotavirus vaccine introduction in Brazil and elsewhere, since the occurrence of either unusual our emerging genotypes may pose a challenge to vaccination strategies.
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spelling Neves, Mayara A. OPinheiro, Helder H. CSilva, Rita do Socorro Uchôa daLinhares, Alexandre da CostaSilva, Luciana Damascena daGabbay, Yvone BenchimolSilva, Mônica Cristina de MoraesLoureiro, Edvaldo Carlos BritoSoares, Luana da SilvaMascarenhas, Joana D'Arc Pereira2017-03-24T17:47:24Z2017-03-24T17:47:24Z2016NEVES, Mayara A. O. et al. High prevalence of G12P[8] rotavirus strains in Rio Branco, Acre, Western Amazon, in the post-rotavirus vaccine introduction period. Journal of Medical Virology, v. 88, n. 5, p. 782-789, May 2016. DOI: https://doi.org/10.1002/jmv.244041096-9071http://patua.iec.gov.br/handle/iec/242910.1002/jmv.24404The present study aimed to provide a molecular characterization of circulating rotavirus (RVA) strains in Rio Branco, Acre, in the post-rotavirus vaccination period, particularly with regard to the emerging, increasingly prevalent G12P[8] genotype. A total of 488 fecal specimens from diarrheic and non-diarrheic children were obtained between January and December 2012. RVA detection was initially performed using enzyme-linked immunosorbent assay (ELISA) method, followed by reverse-transcription polymerase chain reaction (RT-PCR) using specific primers. RVA was detected in 18.3 per cent (44/241) of the children with acute diarrhea and in 1.2 per cent (3/247) of the non-diarrheic children (P  0.001), with overall RVA-positivity of 9.6 per cent (47/488). The most common genotype was G2P[4] with 43.2 per cent (19/44) of the diarrheic cases, followed by G12P[8] (27.3 per cent, 12/44), G3P[6] (18.2 per cent, 8/44), G3P[8] (4.5 per cent, 2/44), and G12P[6] (2.3 per cent, 1/44). G12 samples belonged to lineage III and were from children aged 4û52 months. All of these children had acute diarrhea associated with fever (83.3 per cent, 10/12) and vomiting (66.7 per cent, 8/12). Most of the cases occurred in August (58.3 per cent, 7/12), 75 per cent (9/12) of which having received the full vaccination scheme with RotarixÖ. For the first time G12 was reported at relative high prevalence in Brazil. Our findings warrant further monitoring studies on the molecular characterization of circulating RVA strains after rotavirus vaccine introduction in Brazil and elsewhere, since the occurrence of either unusual our emerging genotypes may pose a challenge to vaccination strategies.University of State of Pará. Center for Biological and Health Sciences. Belém, PA, Brazil.Federal University of Pará, Pará. Center for Tropical Medicine. Belém, PA, Brazil.Municipality Secretary of Health. Rio Branco, Acre, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. 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dc.title.pt_BR.fl_str_mv High prevalence of G12P[8] rotavirus strains in Rio Branco, Acre, Western Amazon, in the post-rotavirus vaccine introduction period
title High prevalence of G12P[8] rotavirus strains in Rio Branco, Acre, Western Amazon, in the post-rotavirus vaccine introduction period
spellingShingle High prevalence of G12P[8] rotavirus strains in Rio Branco, Acre, Western Amazon, in the post-rotavirus vaccine introduction period
Neves, Mayara A. O
Rotavirus / classificação
Rotavirus / genética
Infecções por Rotavirus / epidemiologia
Infecções por Rotavirus / virologia
Diarreia Infantil
Genótipo
Ensaio de Imunoadsorção Enzimática / métodos
Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos
title_short High prevalence of G12P[8] rotavirus strains in Rio Branco, Acre, Western Amazon, in the post-rotavirus vaccine introduction period
title_full High prevalence of G12P[8] rotavirus strains in Rio Branco, Acre, Western Amazon, in the post-rotavirus vaccine introduction period
title_fullStr High prevalence of G12P[8] rotavirus strains in Rio Branco, Acre, Western Amazon, in the post-rotavirus vaccine introduction period
title_full_unstemmed High prevalence of G12P[8] rotavirus strains in Rio Branco, Acre, Western Amazon, in the post-rotavirus vaccine introduction period
title_sort High prevalence of G12P[8] rotavirus strains in Rio Branco, Acre, Western Amazon, in the post-rotavirus vaccine introduction period
author Neves, Mayara A. O
author_facet Neves, Mayara A. O
Pinheiro, Helder H. C
Silva, Rita do Socorro Uchôa da
Linhares, Alexandre da Costa
Silva, Luciana Damascena da
Gabbay, Yvone Benchimol
Silva, Mônica Cristina de Moraes
Loureiro, Edvaldo Carlos Brito
Soares, Luana da Silva
Mascarenhas, Joana D'Arc Pereira
author_role author
author2 Pinheiro, Helder H. C
Silva, Rita do Socorro Uchôa da
Linhares, Alexandre da Costa
Silva, Luciana Damascena da
Gabbay, Yvone Benchimol
Silva, Mônica Cristina de Moraes
Loureiro, Edvaldo Carlos Brito
Soares, Luana da Silva
Mascarenhas, Joana D'Arc Pereira
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Neves, Mayara A. O
Pinheiro, Helder H. C
Silva, Rita do Socorro Uchôa da
Linhares, Alexandre da Costa
Silva, Luciana Damascena da
Gabbay, Yvone Benchimol
Silva, Mônica Cristina de Moraes
Loureiro, Edvaldo Carlos Brito
Soares, Luana da Silva
Mascarenhas, Joana D'Arc Pereira
dc.subject.decsPrimary.pt_BR.fl_str_mv Rotavirus / classificação
Rotavirus / genética
Infecções por Rotavirus / epidemiologia
Infecções por Rotavirus / virologia
Diarreia Infantil
Genótipo
Ensaio de Imunoadsorção Enzimática / métodos
Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos
topic Rotavirus / classificação
Rotavirus / genética
Infecções por Rotavirus / epidemiologia
Infecções por Rotavirus / virologia
Diarreia Infantil
Genótipo
Ensaio de Imunoadsorção Enzimática / métodos
Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos
description The present study aimed to provide a molecular characterization of circulating rotavirus (RVA) strains in Rio Branco, Acre, in the post-rotavirus vaccination period, particularly with regard to the emerging, increasingly prevalent G12P[8] genotype. A total of 488 fecal specimens from diarrheic and non-diarrheic children were obtained between January and December 2012. RVA detection was initially performed using enzyme-linked immunosorbent assay (ELISA) method, followed by reverse-transcription polymerase chain reaction (RT-PCR) using specific primers. RVA was detected in 18.3 per cent (44/241) of the children with acute diarrhea and in 1.2 per cent (3/247) of the non-diarrheic children (P  0.001), with overall RVA-positivity of 9.6 per cent (47/488). The most common genotype was G2P[4] with 43.2 per cent (19/44) of the diarrheic cases, followed by G12P[8] (27.3 per cent, 12/44), G3P[6] (18.2 per cent, 8/44), G3P[8] (4.5 per cent, 2/44), and G12P[6] (2.3 per cent, 1/44). G12 samples belonged to lineage III and were from children aged 4û52 months. All of these children had acute diarrhea associated with fever (83.3 per cent, 10/12) and vomiting (66.7 per cent, 8/12). Most of the cases occurred in August (58.3 per cent, 7/12), 75 per cent (9/12) of which having received the full vaccination scheme with RotarixÖ. For the first time G12 was reported at relative high prevalence in Brazil. Our findings warrant further monitoring studies on the molecular characterization of circulating RVA strains after rotavirus vaccine introduction in Brazil and elsewhere, since the occurrence of either unusual our emerging genotypes may pose a challenge to vaccination strategies.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-03-24T17:47:24Z
dc.date.available.fl_str_mv 2017-03-24T17:47:24Z
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dc.identifier.citation.fl_str_mv NEVES, Mayara A. O. et al. High prevalence of G12P[8] rotavirus strains in Rio Branco, Acre, Western Amazon, in the post-rotavirus vaccine introduction period. Journal of Medical Virology, v. 88, n. 5, p. 782-789, May 2016. DOI: https://doi.org/10.1002/jmv.24404
dc.identifier.uri.fl_str_mv http://patua.iec.gov.br/handle/iec/2429
dc.identifier.issn.-.fl_str_mv 1096-9071
dc.identifier.doi.-.fl_str_mv 10.1002/jmv.24404
identifier_str_mv NEVES, Mayara A. O. et al. High prevalence of G12P[8] rotavirus strains in Rio Branco, Acre, Western Amazon, in the post-rotavirus vaccine introduction period. Journal of Medical Virology, v. 88, n. 5, p. 782-789, May 2016. DOI: https://doi.org/10.1002/jmv.24404
1096-9071
10.1002/jmv.24404
url http://patua.iec.gov.br/handle/iec/2429
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