Experimental Dengue Virus type 4 infection increases the expression of MicroRNAs-15/16, triggering a caspase-induced apoptosis pathway
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/6847 |
Resumo: | The World Health Organization has estimated the annual occurrence of approximately 392 million dengue virus (DENV) infections in more than 100 countries where the virus is endemic, which represents a serious threat to humanity. DENV is a serologic group with four distinct serotypes (DENV-1, DENV-2, DENV-3, and DENV-4) belonging to the genus Flavivirus, in the family Flaviviridae. Dengue is the most widespread mosquito-borne disease in the world. The ~10.7 kb DENV genome encodes three structural proteins (capsid (C), pre-membrane (prM), and envelope (E)) and seven non-structural (NS) proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5). The NS1 protein is a membrane-associated dimer and a secreted, lipid-associated hexamer. Dimeric NS1 is found on membranes both in cellular compartments and cell surfaces. Secreted NS1 (sNS1) is often present in patient serum at very high levels, which correlates with severe dengue symptoms. This study was conducted to discover how the NS1 protein, microRNAs-15/16 (miRNAs-15/16), and apoptosis are related during DENV-4 infection in human liver cell lines. Huh 7.5 and HepG2 cells were infected with DENV-4, and miRNAs-15/16, viral load, NS1 protein, and caspases-3/7 were quantified after different durations of infection. This study demonstrated that miRNAs-15/16 were overexpressed during the infection of HepG2 and Huh 7.5 cells with DENV-4 and had a relationship with NS1 protein expression, viral load, and the activity of caspases-3/7, thus making these miRNAs potential injury markers during DENV infection in human hepatocytes. |
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Casseb, Samir Mansour MoraesMelo, Karla Fabiane Lopes deCarvalho, Carlos Alberto Marques deSantos, Carolina Ramos dosFranco, Edna Crisitna SantosVasconcelos, Pedro Fernando da Costa2023-07-17T17:25:47Z2023-07-17T17:25:47Z2023CASSEB, Samir Mansour Moraes et al. Experimental Dengue Virus type 4 infection increases the expression of MicroRNAs-15/16, triggering a caspase-induced apoptosis pathway. Current Issues in Molecular Biology, v. 45, n. 6, p. 4589-4599, 2023. DOI: https://doi.org/10.3390/cimb45060291.1467-3045https://patua.iec.gov.br/handle/iec/684710.3390/cimb45060291The World Health Organization has estimated the annual occurrence of approximately 392 million dengue virus (DENV) infections in more than 100 countries where the virus is endemic, which represents a serious threat to humanity. DENV is a serologic group with four distinct serotypes (DENV-1, DENV-2, DENV-3, and DENV-4) belonging to the genus Flavivirus, in the family Flaviviridae. Dengue is the most widespread mosquito-borne disease in the world. The ~10.7 kb DENV genome encodes three structural proteins (capsid (C), pre-membrane (prM), and envelope (E)) and seven non-structural (NS) proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5). The NS1 protein is a membrane-associated dimer and a secreted, lipid-associated hexamer. Dimeric NS1 is found on membranes both in cellular compartments and cell surfaces. Secreted NS1 (sNS1) is often present in patient serum at very high levels, which correlates with severe dengue symptoms. This study was conducted to discover how the NS1 protein, microRNAs-15/16 (miRNAs-15/16), and apoptosis are related during DENV-4 infection in human liver cell lines. Huh 7.5 and HepG2 cells were infected with DENV-4, and miRNAs-15/16, viral load, NS1 protein, and caspases-3/7 were quantified after different durations of infection. This study demonstrated that miRNAs-15/16 were overexpressed during the infection of HepG2 and Huh 7.5 cells with DENV-4 and had a relationship with NS1 protein expression, viral load, and the activity of caspases-3/7, thus making these miRNAs potential injury markers during DENV infection in human hepatocytes.CNPq INCT-VER 406360/2022-7.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Federal University of Pará. Oncology Research Center. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.University of Pará State. Department of Pathology. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.University of Pará State. Department of Pathology. Belém, PA, Brazil.engMDPIExperimental Dengue Virus type 4 infection increases the expression of MicroRNAs-15/16, triggering a caspase-induced apoptosis pathwayinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleDengueVírus da Dengue / patogenicidadeProteínas não Estruturais Virais / genéticaMicroRNAs / genéticainfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALExperimental Dengue Virus Type 4 Infection Increases the Expression of MicroRNAs1516, Triggering a CaspaseInduced Apoptosis Pathway.pdfExperimental Dengue Virus Type 4 Infection Increases the Expression of MicroRNAs1516, Triggering a CaspaseInduced Apoptosis Pathway.pdfapplication/pdf2549145https://patua.iec.gov.br/bitstreams/20b400ff-1902-423b-8cb6-d4df971dbf2a/download61e1510dd797613510f91c6099d36875MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/3beb0f58-3d91-41b9-9ebd-90d93702b1b3/download11832eea31b16df8613079d742d61793MD52TEXTExperimental Dengue Virus Type 4 Infection Increases the Expression of MicroRNAs1516, Triggering a CaspaseInduced Apoptosis Pathway.pdf.txtExperimental Dengue Virus Type 4 Infection Increases the Expression of MicroRNAs1516, Triggering a CaspaseInduced Apoptosis Pathway.pdf.txtExtracted texttext/plain50805https://patua.iec.gov.br/bitstreams/0dcda9af-dda0-4aea-88bc-a98b7ac57457/downloada48fb9e8dc213a73085c55f46000712dMD53THUMBNAILExperimental Dengue Virus Type 4 Infection Increases the Expression of MicroRNAs1516, Triggering a CaspaseInduced Apoptosis Pathway.pdf.jpgExperimental Dengue Virus Type 4 Infection Increases the Expression of MicroRNAs1516, Triggering a CaspaseInduced Apoptosis Pathway.pdf.jpgGenerated Thumbnailimage/jpeg6028https://patua.iec.gov.br/bitstreams/860faf73-e591-4520-92c6-3ceff1809e73/download58e6cb6f0dad998d0ed030efefa56734MD54iec/68472023-07-17 17:43:01.899oai:patua.iec.gov.br:iec/6847https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2023-07-17T17:43:01Repositório Digital do Instituto Evandro Chagas (Patuá) - 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dc.title.pt_BR.fl_str_mv |
Experimental Dengue Virus type 4 infection increases the expression of MicroRNAs-15/16, triggering a caspase-induced apoptosis pathway |
title |
Experimental Dengue Virus type 4 infection increases the expression of MicroRNAs-15/16, triggering a caspase-induced apoptosis pathway |
spellingShingle |
Experimental Dengue Virus type 4 infection increases the expression of MicroRNAs-15/16, triggering a caspase-induced apoptosis pathway Casseb, Samir Mansour Moraes Dengue Vírus da Dengue / patogenicidade Proteínas não Estruturais Virais / genética MicroRNAs / genética |
title_short |
Experimental Dengue Virus type 4 infection increases the expression of MicroRNAs-15/16, triggering a caspase-induced apoptosis pathway |
title_full |
Experimental Dengue Virus type 4 infection increases the expression of MicroRNAs-15/16, triggering a caspase-induced apoptosis pathway |
title_fullStr |
Experimental Dengue Virus type 4 infection increases the expression of MicroRNAs-15/16, triggering a caspase-induced apoptosis pathway |
title_full_unstemmed |
Experimental Dengue Virus type 4 infection increases the expression of MicroRNAs-15/16, triggering a caspase-induced apoptosis pathway |
title_sort |
Experimental Dengue Virus type 4 infection increases the expression of MicroRNAs-15/16, triggering a caspase-induced apoptosis pathway |
author |
Casseb, Samir Mansour Moraes |
author_facet |
Casseb, Samir Mansour Moraes Melo, Karla Fabiane Lopes de Carvalho, Carlos Alberto Marques de Santos, Carolina Ramos dos Franco, Edna Crisitna Santos Vasconcelos, Pedro Fernando da Costa |
author_role |
author |
author2 |
Melo, Karla Fabiane Lopes de Carvalho, Carlos Alberto Marques de Santos, Carolina Ramos dos Franco, Edna Crisitna Santos Vasconcelos, Pedro Fernando da Costa |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Casseb, Samir Mansour Moraes Melo, Karla Fabiane Lopes de Carvalho, Carlos Alberto Marques de Santos, Carolina Ramos dos Franco, Edna Crisitna Santos Vasconcelos, Pedro Fernando da Costa |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Dengue Vírus da Dengue / patogenicidade Proteínas não Estruturais Virais / genética MicroRNAs / genética |
topic |
Dengue Vírus da Dengue / patogenicidade Proteínas não Estruturais Virais / genética MicroRNAs / genética |
description |
The World Health Organization has estimated the annual occurrence of approximately 392 million dengue virus (DENV) infections in more than 100 countries where the virus is endemic, which represents a serious threat to humanity. DENV is a serologic group with four distinct serotypes (DENV-1, DENV-2, DENV-3, and DENV-4) belonging to the genus Flavivirus, in the family Flaviviridae. Dengue is the most widespread mosquito-borne disease in the world. The ~10.7 kb DENV genome encodes three structural proteins (capsid (C), pre-membrane (prM), and envelope (E)) and seven non-structural (NS) proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5). The NS1 protein is a membrane-associated dimer and a secreted, lipid-associated hexamer. Dimeric NS1 is found on membranes both in cellular compartments and cell surfaces. Secreted NS1 (sNS1) is often present in patient serum at very high levels, which correlates with severe dengue symptoms. This study was conducted to discover how the NS1 protein, microRNAs-15/16 (miRNAs-15/16), and apoptosis are related during DENV-4 infection in human liver cell lines. Huh 7.5 and HepG2 cells were infected with DENV-4, and miRNAs-15/16, viral load, NS1 protein, and caspases-3/7 were quantified after different durations of infection. This study demonstrated that miRNAs-15/16 were overexpressed during the infection of HepG2 and Huh 7.5 cells with DENV-4 and had a relationship with NS1 protein expression, viral load, and the activity of caspases-3/7, thus making these miRNAs potential injury markers during DENV infection in human hepatocytes. |
publishDate |
2023 |
dc.date.accessioned.fl_str_mv |
2023-07-17T17:25:47Z |
dc.date.available.fl_str_mv |
2023-07-17T17:25:47Z |
dc.date.issued.fl_str_mv |
2023 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CASSEB, Samir Mansour Moraes et al. Experimental Dengue Virus type 4 infection increases the expression of MicroRNAs-15/16, triggering a caspase-induced apoptosis pathway. Current Issues in Molecular Biology, v. 45, n. 6, p. 4589-4599, 2023. DOI: https://doi.org/10.3390/cimb45060291. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/6847 |
dc.identifier.issn.-.fl_str_mv |
1467-3045 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.3390/cimb45060291 |
identifier_str_mv |
CASSEB, Samir Mansour Moraes et al. Experimental Dengue Virus type 4 infection increases the expression of MicroRNAs-15/16, triggering a caspase-induced apoptosis pathway. Current Issues in Molecular Biology, v. 45, n. 6, p. 4589-4599, 2023. DOI: https://doi.org/10.3390/cimb45060291. 1467-3045 10.3390/cimb45060291 |
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https://patua.iec.gov.br/handle/iec/6847 |
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MDPI |
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