Drosha, DGCR8, and Dicer mRNAs are down-regulated in human cells infected with dengue virus 4, and play a role in viral pathogenesis
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/2097 |
Resumo: | Dengue virus (DENV) and its four serotypes (DENV1- 4) belong to the Flavivirus genus of the Flaviviridae family. DENV infection is a life-threatening disease, which results in up to 20,000 deaths each year. Viruses have been shown to encode trans-regulatory small RNAs, or microRNAs (miRNAs), which bind to messenger RNA and negatively regulate host or viral gene expression. During DENV infections, miRNAs interact with proteins in the RNAi pathway, and are processed by ribonucleases such as Dicer and Drosha. This study aims to investigate Drosha, DGCR8, and Dicer expression levels in human A-549 cells following DENV4 infection. DENV4 infected A-549 cells were collected daily for 5 days, and RNA was extracted to quantify viral load. Gene expression of Drosha, Dicer, and DGCR8 was determined using quantitative PCR (RT-qPCR). We found that DENV4 infection exhibited the highest viral load 3 days post-infection. Dicer, Drosha, and DGCR8 showed reduced expression following S.M.M. Casseb et al. 2 Genetics and Molecular Research 15 (2): gmr.15027891 ©FUNPEC-RP www.funpecrp.com.br DENV4 infection as compared with negative controls. In addition, we hypothesize that reduced expression of DGCR8 may not only be related to miRNA biogenesis, but also other small RNAs. This study may change our understanding regarding the relationship between host cells and the dengue virus. |
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Casseb, Samir Mansour MoraesSimith, Darlene de BritoMelo, Karla Fabiane Lopes deMendonça, M. HSantos, A. C. MCarvalho, Valéria LimaCruz, Ana Cecília RibeiroVasconcelos, Pedro Fernando da Costa2016-06-09T12:01:10Z2016-06-09T12:01:10Z2016CASSEB, Samir Mansour Moraes et al. Drosha, DGCR8, and Dicer mRNAs are down-regulated in human cells infected with dengue virus 4, and play a role in viral pathogenesis. Genetics and Molecular Research, v. 15, n. 2, p. 1-8, May 2016.1676-5680https://patua.iec.gov.br/handle/iec/209710.4238/GMR.15027891Dengue virus (DENV) and its four serotypes (DENV1- 4) belong to the Flavivirus genus of the Flaviviridae family. DENV infection is a life-threatening disease, which results in up to 20,000 deaths each year. Viruses have been shown to encode trans-regulatory small RNAs, or microRNAs (miRNAs), which bind to messenger RNA and negatively regulate host or viral gene expression. During DENV infections, miRNAs interact with proteins in the RNAi pathway, and are processed by ribonucleases such as Dicer and Drosha. This study aims to investigate Drosha, DGCR8, and Dicer expression levels in human A-549 cells following DENV4 infection. DENV4 infected A-549 cells were collected daily for 5 days, and RNA was extracted to quantify viral load. Gene expression of Drosha, Dicer, and DGCR8 was determined using quantitative PCR (RT-qPCR). We found that DENV4 infection exhibited the highest viral load 3 days post-infection. Dicer, Drosha, and DGCR8 showed reduced expression following S.M.M. Casseb et al. 2 Genetics and Molecular Research 15 (2): gmr.15027891 ©FUNPEC-RP www.funpecrp.com.br DENV4 infection as compared with negative controls. In addition, we hypothesize that reduced expression of DGCR8 may not only be related to miRNA biogenesis, but also other small RNAs. This study may change our understanding regarding the relationship between host cells and the dengue virus.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.application/pdfengFundação de Ribeirão Preto para PesquisaDrosha, DGCR8, and Dicer mRNAs are down-regulated in human cells infected with dengue virus 4, and play a role in viral pathogenesisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleDengueVírus da Dengue / genéticaRNA Viral / genéticaMicroRNAs / metabolismoReplicação ViralProteínas de Ligação a RNAArbovirusDGCR8Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodosinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALDrosha, DGCR8, and Dicer mRNAs are down-regulated in human cells infected with dengue virus 4, and play a role in viral pathogenesis.pdfapplication/pdf1999943https://patua.iec.gov.br/bitstreams/d41a991c-103d-4059-8f1c-6d91c01da33e/download54a00beef585a7866d44507f34e06e02MD51TEXTfile_1.pdf.txtfile_1.pdf.txtExtracted texttext/plain19066https://patua.iec.gov.br/bitstreams/915d0652-f231-42c8-9aeb-83a4225d8ffc/download9b52e7ad6e3c958ae4a868cb3ccbcd46MD52Drosha, DGCR8, and Dicer mRNAs are down-regulated in human cells infected with dengue virus 4, and play a role in viral pathogenesis.pdf.txtDrosha, DGCR8, and Dicer mRNAs are down-regulated in human cells infected with dengue virus 4, and play a role in viral pathogenesis.pdf.txtExtracted texttext/plain19184https://patua.iec.gov.br/bitstreams/c10ca832-1ec4-4d3a-bc2f-3878ee660043/download132942696861ba67cd2c3741314dac5bMD57THUMBNAILfile_1.pdf.jpgfile_1.pdf.jpgIM Thumbnailimage/jpeg4298https://patua.iec.gov.br/bitstreams/1b65d3d7-f3f1-4d6a-8cc0-13ecebeea37c/download7cb236282c573dc9e55a362c3cb37b93MD53Drosha, DGCR8, and Dicer mRNAs are down-regulated in human cells infected with dengue virus 4, and play a role in viral pathogenesis.pdf.jpgDrosha, DGCR8, and Dicer mRNAs are down-regulated in human cells infected with dengue virus 4, and play a role in viral pathogenesis.pdf.jpgGenerated Thumbnailimage/jpeg4385https://patua.iec.gov.br/bitstreams/c0cb442c-2d25-4733-96c6-559b26d194d6/download83bd63c858ae4ae8a4ade3258bca389bMD58LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv |
Drosha, DGCR8, and Dicer mRNAs are down-regulated in human cells infected with dengue virus 4, and play a role in viral pathogenesis |
title |
Drosha, DGCR8, and Dicer mRNAs are down-regulated in human cells infected with dengue virus 4, and play a role in viral pathogenesis |
spellingShingle |
Drosha, DGCR8, and Dicer mRNAs are down-regulated in human cells infected with dengue virus 4, and play a role in viral pathogenesis Casseb, Samir Mansour Moraes Dengue Vírus da Dengue / genética RNA Viral / genética MicroRNAs / metabolismo Replicação Viral Proteínas de Ligação a RNA Arbovirus DGCR8 Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos |
title_short |
Drosha, DGCR8, and Dicer mRNAs are down-regulated in human cells infected with dengue virus 4, and play a role in viral pathogenesis |
title_full |
Drosha, DGCR8, and Dicer mRNAs are down-regulated in human cells infected with dengue virus 4, and play a role in viral pathogenesis |
title_fullStr |
Drosha, DGCR8, and Dicer mRNAs are down-regulated in human cells infected with dengue virus 4, and play a role in viral pathogenesis |
title_full_unstemmed |
Drosha, DGCR8, and Dicer mRNAs are down-regulated in human cells infected with dengue virus 4, and play a role in viral pathogenesis |
title_sort |
Drosha, DGCR8, and Dicer mRNAs are down-regulated in human cells infected with dengue virus 4, and play a role in viral pathogenesis |
author |
Casseb, Samir Mansour Moraes |
author_facet |
Casseb, Samir Mansour Moraes Simith, Darlene de Brito Melo, Karla Fabiane Lopes de Mendonça, M. H Santos, A. C. M Carvalho, Valéria Lima Cruz, Ana Cecília Ribeiro Vasconcelos, Pedro Fernando da Costa |
author_role |
author |
author2 |
Simith, Darlene de Brito Melo, Karla Fabiane Lopes de Mendonça, M. H Santos, A. C. M Carvalho, Valéria Lima Cruz, Ana Cecília Ribeiro Vasconcelos, Pedro Fernando da Costa |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Casseb, Samir Mansour Moraes Simith, Darlene de Brito Melo, Karla Fabiane Lopes de Mendonça, M. H Santos, A. C. M Carvalho, Valéria Lima Cruz, Ana Cecília Ribeiro Vasconcelos, Pedro Fernando da Costa |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Dengue Vírus da Dengue / genética RNA Viral / genética MicroRNAs / metabolismo Replicação Viral Proteínas de Ligação a RNA Arbovirus DGCR8 Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos |
topic |
Dengue Vírus da Dengue / genética RNA Viral / genética MicroRNAs / metabolismo Replicação Viral Proteínas de Ligação a RNA Arbovirus DGCR8 Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos |
description |
Dengue virus (DENV) and its four serotypes (DENV1- 4) belong to the Flavivirus genus of the Flaviviridae family. DENV infection is a life-threatening disease, which results in up to 20,000 deaths each year. Viruses have been shown to encode trans-regulatory small RNAs, or microRNAs (miRNAs), which bind to messenger RNA and negatively regulate host or viral gene expression. During DENV infections, miRNAs interact with proteins in the RNAi pathway, and are processed by ribonucleases such as Dicer and Drosha. This study aims to investigate Drosha, DGCR8, and Dicer expression levels in human A-549 cells following DENV4 infection. DENV4 infected A-549 cells were collected daily for 5 days, and RNA was extracted to quantify viral load. Gene expression of Drosha, Dicer, and DGCR8 was determined using quantitative PCR (RT-qPCR). We found that DENV4 infection exhibited the highest viral load 3 days post-infection. Dicer, Drosha, and DGCR8 showed reduced expression following S.M.M. Casseb et al. 2 Genetics and Molecular Research 15 (2): gmr.15027891 ©FUNPEC-RP www.funpecrp.com.br DENV4 infection as compared with negative controls. In addition, we hypothesize that reduced expression of DGCR8 may not only be related to miRNA biogenesis, but also other small RNAs. This study may change our understanding regarding the relationship between host cells and the dengue virus. |
publishDate |
2016 |
dc.date.accessioned.fl_str_mv |
2016-06-09T12:01:10Z |
dc.date.available.fl_str_mv |
2016-06-09T12:01:10Z |
dc.date.issued.fl_str_mv |
2016 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CASSEB, Samir Mansour Moraes et al. Drosha, DGCR8, and Dicer mRNAs are down-regulated in human cells infected with dengue virus 4, and play a role in viral pathogenesis. Genetics and Molecular Research, v. 15, n. 2, p. 1-8, May 2016. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/2097 |
dc.identifier.issn.-.fl_str_mv |
1676-5680 |
dc.identifier.doi.-.fl_str_mv |
10.4238/GMR.15027891 |
identifier_str_mv |
CASSEB, Samir Mansour Moraes et al. Drosha, DGCR8, and Dicer mRNAs are down-regulated in human cells infected with dengue virus 4, and play a role in viral pathogenesis. Genetics and Molecular Research, v. 15, n. 2, p. 1-8, May 2016. 1676-5680 10.4238/GMR.15027891 |
url |
https://patua.iec.gov.br/handle/iec/2097 |
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eng |
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eng |
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openAccess |
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Fundação de Ribeirão Preto para Pesquisa |
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Fundação de Ribeirão Preto para Pesquisa |
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