Clonality and antimicrobial resistance gene profiles of multidrug- resistant Salmonella enterica serovar infantis isolates from four public hospitals in Rio de Janeiro, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/1018 |
Resumo: | In Brazil, Salmonella enterica serovar Infantis resistant to various antimicrobials, including cephalosporins, has been identified as an etiological agent of severe gastroenteritis in hospitalized children since 1994. In this study, 35 serovar Infantis strains, isolated from children admitted to four different Rio de Janeiro, Brazil, hospitals between 1996 and 2001, were characterized by pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptibility testing in order to determine their genetic relatedness and antimicrobial resistance profiles. Thirty-four serovar Infantis strains were resistant to at least two antibiotic classes, and all 35 strains were susceptible to fluoroquinolones, cephamycin, and carbapenem. Extended-spectrum beta-lactamase (ESBL) screening by double-disk diffusion indicated that 32 serovar Infantis strains (91.4%) produced betalactamases that were inhibited by clavulanic acid. Antimicrobial resistance gene profiles were determined by PCR for a subset of 11 multidrug-resistant serovar Infantis strains, and putative ESBLs were detected by isoelectric focusing. Ten serovar Infantis strains carried blaTEM, catI, ant(3 )Ia and/or ant(3 )Ib, sulI and/or sulII, and tet(D) genes as well as an integron-associated aac(6)-Iq cassette. Eight strains possessed at least four different beta-lactamases with pI profiles that confirmed the presence of both ESBLs and non-ESBLs. Our PFGE profiles indicated that 33 serovar Infantis strains isolated from Rio de Janeiro hospitals came from the same genetic lineage. |
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Fonseca, E. LMykytczuk, O. LAsensi, M. DReis, E. M. FFerraz, L. RRamos, Francisco Lúzio de PaulaNg, L. KRodrigues, D. P2016-01-26T11:41:03Z2016-01-26T11:41:03Z2006FONSECA, E. L et al. Clonality and antimicrobial resistance gene profiles of multidrug- resistant Salmonella enterica serovar infantis isolates from four public hospitals in Rio de Janeiro, Brazil. Journal of Clinical Microbiology, v. 44, n.8, p. 2767-2772, Aug. 20060095-1137https://patua.iec.gov.br/handle/iec/101810.1128/JCM.01916-05In Brazil, Salmonella enterica serovar Infantis resistant to various antimicrobials, including cephalosporins, has been identified as an etiological agent of severe gastroenteritis in hospitalized children since 1994. In this study, 35 serovar Infantis strains, isolated from children admitted to four different Rio de Janeiro, Brazil, hospitals between 1996 and 2001, were characterized by pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptibility testing in order to determine their genetic relatedness and antimicrobial resistance profiles. Thirty-four serovar Infantis strains were resistant to at least two antibiotic classes, and all 35 strains were susceptible to fluoroquinolones, cephamycin, and carbapenem. Extended-spectrum beta-lactamase (ESBL) screening by double-disk diffusion indicated that 32 serovar Infantis strains (91.4%) produced betalactamases that were inhibited by clavulanic acid. Antimicrobial resistance gene profiles were determined by PCR for a subset of 11 multidrug-resistant serovar Infantis strains, and putative ESBLs were detected by isoelectric focusing. Ten serovar Infantis strains carried blaTEM, catI, ant(3 )Ia and/or ant(3 )Ib, sulI and/or sulII, and tet(D) genes as well as an integron-associated aac(6)-Iq cassette. Eight strains possessed at least four different beta-lactamases with pI profiles that confirmed the presence of both ESBLs and non-ESBLs. Our PFGE profiles indicated that 33 serovar Infantis strains isolated from Rio de Janeiro hospitals came from the same genetic lineage.Foundation Oswaldo Cruz . Oswaldo Cruz Institute. Bacteriology Department. Rio de Janeiro, RJ, Brazil.Public Health Agency of Canada. National Microbiology Laboratory. Manitoba, Canada / University of Manitoba. Faculty of Medicine. Departament of Medical Microbiology. Manitoba, Canada.Foundation Oswaldo Cruz . Oswaldo Cruz Institute. Bacteriology Department. Rio de Janeiro, RJ, Brazil.Foundation Oswaldo Cruz . Oswaldo Cruz Institute. Bacteriology Department. Rio de Janeiro, RJ, Brazil.Public Health Laboratory. Brasília, DF, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Public Health Agency of Canada. National Microbiology Laboratory. Manitoba, Canada / University of Manitoba. Faculty of Medicine. Departament of Medical Microbiology. Manitoba, Canada.Foundation Oswaldo Cruz . Oswaldo Cruz Institute. Bacteriology Department. Rio de Janeiro, RJ, Brazil.application/pdfengAmerican Society for MicrobiologyClonality and antimicrobial resistance gene profiles of multidrug- resistant Salmonella enterica serovar infantis isolates from four public hospitals in Rio de Janeiro, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenes MDRHospitais MunicipaisSalmonella Enterica / isolamento & purificaçãoRio de Janeiro (RJ)info:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALClonality and antimicrobial resistance gene profiles of multidrug- resistant Salmonella enterica serovar infantis isolates from four public hospitals in Rio de Janeiro, Brazil .pdfapplication/pdf133771https://patua.iec.gov.br/bitstreams/61e29a5f-8332-4694-89b3-308789038270/download76753e7b477ce939829b3e8a24f4e4caMD51TEXTfile_1.pdf.txtfile_1.pdf.txtExtracted texttext/plain38945https://patua.iec.gov.br/bitstreams/53689e43-8131-4e92-bf39-7bd65c2a16f9/download4bab6eabcdcde43b0cc7c1fb51864ecaMD52Clonality and antimicrobial resistance gene profiles of multidrug- resistant Salmonella enterica serovar infantis isolates from four public hospitals in Rio de Janeiro, Brazil .pdf.txtClonality and antimicrobial resistance gene profiles of multidrug- resistant Salmonella enterica serovar infantis isolates from four public hospitals in Rio de Janeiro, Brazil .pdf.txtExtracted texttext/plain39254https://patua.iec.gov.br/bitstreams/f73c1061-d30f-4bb3-a07b-8f405425a1cb/download064c7ab4b5af2e0af197b646e5cbfca0MD56THUMBNAILClonality and antimicrobial resistance gene profiles of multidrug- resistant Salmonella enterica serovar infantis isolates from four public hospitals in Rio de Janeiro, Brazil .pdf.jpgClonality and antimicrobial resistance gene profiles of multidrug- resistant Salmonella enterica serovar infantis isolates from four public hospitals in Rio de Janeiro, Brazil .pdf.jpgGenerated Thumbnailimage/jpeg6166https://patua.iec.gov.br/bitstreams/9802fbac-8edc-4bf3-8e9c-e1cc9bc5b827/downloadf1d4ffaf2c7078082b128772dbf7c9efMD57LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv |
Clonality and antimicrobial resistance gene profiles of multidrug- resistant Salmonella enterica serovar infantis isolates from four public hospitals in Rio de Janeiro, Brazil |
title |
Clonality and antimicrobial resistance gene profiles of multidrug- resistant Salmonella enterica serovar infantis isolates from four public hospitals in Rio de Janeiro, Brazil |
spellingShingle |
Clonality and antimicrobial resistance gene profiles of multidrug- resistant Salmonella enterica serovar infantis isolates from four public hospitals in Rio de Janeiro, Brazil Fonseca, E. L Genes MDR Hospitais Municipais Salmonella Enterica / isolamento & purificação Rio de Janeiro (RJ) |
title_short |
Clonality and antimicrobial resistance gene profiles of multidrug- resistant Salmonella enterica serovar infantis isolates from four public hospitals in Rio de Janeiro, Brazil |
title_full |
Clonality and antimicrobial resistance gene profiles of multidrug- resistant Salmonella enterica serovar infantis isolates from four public hospitals in Rio de Janeiro, Brazil |
title_fullStr |
Clonality and antimicrobial resistance gene profiles of multidrug- resistant Salmonella enterica serovar infantis isolates from four public hospitals in Rio de Janeiro, Brazil |
title_full_unstemmed |
Clonality and antimicrobial resistance gene profiles of multidrug- resistant Salmonella enterica serovar infantis isolates from four public hospitals in Rio de Janeiro, Brazil |
title_sort |
Clonality and antimicrobial resistance gene profiles of multidrug- resistant Salmonella enterica serovar infantis isolates from four public hospitals in Rio de Janeiro, Brazil |
author |
Fonseca, E. L |
author_facet |
Fonseca, E. L Mykytczuk, O. L Asensi, M. D Reis, E. M. F Ferraz, L. R Ramos, Francisco Lúzio de Paula Ng, L. K Rodrigues, D. P |
author_role |
author |
author2 |
Mykytczuk, O. L Asensi, M. D Reis, E. M. F Ferraz, L. R Ramos, Francisco Lúzio de Paula Ng, L. K Rodrigues, D. P |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Fonseca, E. L Mykytczuk, O. L Asensi, M. D Reis, E. M. F Ferraz, L. R Ramos, Francisco Lúzio de Paula Ng, L. K Rodrigues, D. P |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Genes MDR Hospitais Municipais Salmonella Enterica / isolamento & purificação Rio de Janeiro (RJ) |
topic |
Genes MDR Hospitais Municipais Salmonella Enterica / isolamento & purificação Rio de Janeiro (RJ) |
description |
In Brazil, Salmonella enterica serovar Infantis resistant to various antimicrobials, including cephalosporins, has been identified as an etiological agent of severe gastroenteritis in hospitalized children since 1994. In this study, 35 serovar Infantis strains, isolated from children admitted to four different Rio de Janeiro, Brazil, hospitals between 1996 and 2001, were characterized by pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptibility testing in order to determine their genetic relatedness and antimicrobial resistance profiles. Thirty-four serovar Infantis strains were resistant to at least two antibiotic classes, and all 35 strains were susceptible to fluoroquinolones, cephamycin, and carbapenem. Extended-spectrum beta-lactamase (ESBL) screening by double-disk diffusion indicated that 32 serovar Infantis strains (91.4%) produced betalactamases that were inhibited by clavulanic acid. Antimicrobial resistance gene profiles were determined by PCR for a subset of 11 multidrug-resistant serovar Infantis strains, and putative ESBLs were detected by isoelectric focusing. Ten serovar Infantis strains carried blaTEM, catI, ant(3 )Ia and/or ant(3 )Ib, sulI and/or sulII, and tet(D) genes as well as an integron-associated aac(6)-Iq cassette. Eight strains possessed at least four different beta-lactamases with pI profiles that confirmed the presence of both ESBLs and non-ESBLs. Our PFGE profiles indicated that 33 serovar Infantis strains isolated from Rio de Janeiro hospitals came from the same genetic lineage. |
publishDate |
2006 |
dc.date.issued.fl_str_mv |
2006 |
dc.date.accessioned.fl_str_mv |
2016-01-26T11:41:03Z |
dc.date.available.fl_str_mv |
2016-01-26T11:41:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
FONSECA, E. L et al. Clonality and antimicrobial resistance gene profiles of multidrug- resistant Salmonella enterica serovar infantis isolates from four public hospitals in Rio de Janeiro, Brazil. Journal of Clinical Microbiology, v. 44, n.8, p. 2767-2772, Aug. 2006 |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/1018 |
dc.identifier.issn.-.fl_str_mv |
0095-1137 |
dc.identifier.doi.-.fl_str_mv |
10.1128/JCM.01916-05 |
identifier_str_mv |
FONSECA, E. L et al. Clonality and antimicrobial resistance gene profiles of multidrug- resistant Salmonella enterica serovar infantis isolates from four public hospitals in Rio de Janeiro, Brazil. Journal of Clinical Microbiology, v. 44, n.8, p. 2767-2772, Aug. 2006 0095-1137 10.1128/JCM.01916-05 |
url |
https://patua.iec.gov.br/handle/iec/1018 |
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eng |
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eng |
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openAccess |
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American Society for Microbiology |
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American Society for Microbiology |
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