Polymorphism in the circumsporozoite protein of the human malaria parasite Plasmodium vivax
Autor(a) principal: | |
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Data de Publicação: | 1992 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/2593 |
Resumo: | The circumsporozoite (CS) protein that covers the surface of infectious sporozoites is a candidate antigen in malaria vaccine development. To determine the extent of B- and T-epitope polymorphism and to understand the mechanisms of antigenic variability, we have characterized the CS protein gene of Plasmodium vivax from field isolates representing geographically distant regions of Papua New Guinea (PNG) and Brazil. In the central repeat region of the CS protein, in addition to variation in the number of repeats, an array of mutations was observed which suggests that point mutations have led to the emergence of the variant CS repeat sequence ANGA(G/D)(N/D)QPG from GDRA(D/A)GQPA. Outside the repeat region of the protein, the nonsilent nucleotide substitutions of independent origin are localized in three domains of the protein that either harbor known T-cell determinants or are analogous to the Plasmodium falciparum immunodominant determinants, Th2R and Th3R. We have found that, with the exception of one CS clone sequence that was shared by one P. vivax isolate each from PNG and Brazil, the P. vivax CS protein types can be grouped into Papuan and Brazilian types. These results suggest that an in-depth study of parasite population dynamics is required before field trials for vaccine formulations based on polymorphic immunodominant determinants are conducted. |
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Qari, Shoukat HGoldman, Ira FPóvoa, Marinete MarinsDi Santi, SylviaAlpers, Michael PLal, Altaf A2017-06-09T12:55:54Z2017-06-09T12:55:54Z1992QARI, Shoukat H. et al. Polymorphism in the circumsporozoite protein of the human malaria parasite Plasmodium vivax. Molecular and Biochemical Parasitology, v. 55, p. 105-114, 1992.0166-6851https://patua.iec.gov.br/handle/iec/2593The circumsporozoite (CS) protein that covers the surface of infectious sporozoites is a candidate antigen in malaria vaccine development. To determine the extent of B- and T-epitope polymorphism and to understand the mechanisms of antigenic variability, we have characterized the CS protein gene of Plasmodium vivax from field isolates representing geographically distant regions of Papua New Guinea (PNG) and Brazil. In the central repeat region of the CS protein, in addition to variation in the number of repeats, an array of mutations was observed which suggests that point mutations have led to the emergence of the variant CS repeat sequence ANGA(G/D)(N/D)QPG from GDRA(D/A)GQPA. Outside the repeat region of the protein, the nonsilent nucleotide substitutions of independent origin are localized in three domains of the protein that either harbor known T-cell determinants or are analogous to the Plasmodium falciparum immunodominant determinants, Th2R and Th3R. We have found that, with the exception of one CS clone sequence that was shared by one P. vivax isolate each from PNG and Brazil, the P. vivax CS protein types can be grouped into Papuan and Brazilian types. These results suggest that an in-depth study of parasite population dynamics is required before field trials for vaccine formulations based on polymorphic immunodominant determinants are conducted.Centers for Disease Control, Public Health Services. U.S. Department of Health and Human Services. Division of Parasitic Diseases, National Center for Infectious Diseases. Malaria Branch. Atlanta GA, USA.Centers for Disease Control, Public Health Services. U.S. Department of Health and Human Services. Division of Parasitic Diseases, National Center for Infectious Diseases. Malaria Branch. Atlanta GA, USA.Ministério da Saúde. Fundação Nacional de Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Governo do Estado de São Paulo. Superintendência de Controle de Endemias. Malaria Department. São Paulo, SP, Brazil.Papua New Guinea Institute of Medical Research. Goroka, PNG.engElsevierPolymorphism in the circumsporozoite protein of the human malaria parasite Plasmodium vivaxinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMalária VivaxPlasmodium vivaxTécnicas de Genotipageminfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECTHUMBNAILPolymorphism in the circumsporozoite protein of the human malaria parasite Plasmodium vivax.pdf.jpgPolymorphism in the circumsporozoite protein of the human malaria parasite Plasmodium vivax.pdf.jpgGenerated Thumbnailimage/jpeg5900https://patua.iec.gov.br/bitstreams/0c4481ab-1985-41be-adb9-a494ee171293/downloadc4c3534af5fca970f3a446b8184cd468MD56ORIGINALPolymorphism in the circumsporozoite protein of the human malaria parasite Plasmodium vivax.pdfPolymorphism in the circumsporozoite protein of the human malaria parasite Plasmodium vivax.pdfapplication/pdf700064https://patua.iec.gov.br/bitstreams/a27e937b-40aa-4f66-980e-e079257d8e96/download5c78657577ef2ca9ea98cffdf53ec25cMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/cb307076-129a-4ce9-b63a-fa0bbbf5bef3/download52f1732ea66fbd1123abe39f5373b797MD52TEXTPolymorphism in the circumsporozoite protein of the human malaria parasite Plasmodium vivax.pdf.txtPolymorphism in the circumsporozoite protein of the human malaria parasite Plasmodium vivax.pdf.txtExtracted texttext/plain33889https://patua.iec.gov.br/bitstreams/2d583303-7775-4953-a5df-69cd37e346b9/download6212678af14eab2e997e08428c6b2609MD55iec/25932022-10-20 21:25:23.499oai:patua.iec.gov.br:iec/2593https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T21:25:23Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4= |
dc.title.pt_BR.fl_str_mv |
Polymorphism in the circumsporozoite protein of the human malaria parasite Plasmodium vivax |
title |
Polymorphism in the circumsporozoite protein of the human malaria parasite Plasmodium vivax |
spellingShingle |
Polymorphism in the circumsporozoite protein of the human malaria parasite Plasmodium vivax Qari, Shoukat H Malária Vivax Plasmodium vivax Técnicas de Genotipagem |
title_short |
Polymorphism in the circumsporozoite protein of the human malaria parasite Plasmodium vivax |
title_full |
Polymorphism in the circumsporozoite protein of the human malaria parasite Plasmodium vivax |
title_fullStr |
Polymorphism in the circumsporozoite protein of the human malaria parasite Plasmodium vivax |
title_full_unstemmed |
Polymorphism in the circumsporozoite protein of the human malaria parasite Plasmodium vivax |
title_sort |
Polymorphism in the circumsporozoite protein of the human malaria parasite Plasmodium vivax |
author |
Qari, Shoukat H |
author_facet |
Qari, Shoukat H Goldman, Ira F Póvoa, Marinete Marins Di Santi, Sylvia Alpers, Michael P Lal, Altaf A |
author_role |
author |
author2 |
Goldman, Ira F Póvoa, Marinete Marins Di Santi, Sylvia Alpers, Michael P Lal, Altaf A |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Qari, Shoukat H Goldman, Ira F Póvoa, Marinete Marins Di Santi, Sylvia Alpers, Michael P Lal, Altaf A |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Malária Vivax Plasmodium vivax Técnicas de Genotipagem |
topic |
Malária Vivax Plasmodium vivax Técnicas de Genotipagem |
description |
The circumsporozoite (CS) protein that covers the surface of infectious sporozoites is a candidate antigen in malaria vaccine development. To determine the extent of B- and T-epitope polymorphism and to understand the mechanisms of antigenic variability, we have characterized the CS protein gene of Plasmodium vivax from field isolates representing geographically distant regions of Papua New Guinea (PNG) and Brazil. In the central repeat region of the CS protein, in addition to variation in the number of repeats, an array of mutations was observed which suggests that point mutations have led to the emergence of the variant CS repeat sequence ANGA(G/D)(N/D)QPG from GDRA(D/A)GQPA. Outside the repeat region of the protein, the nonsilent nucleotide substitutions of independent origin are localized in three domains of the protein that either harbor known T-cell determinants or are analogous to the Plasmodium falciparum immunodominant determinants, Th2R and Th3R. We have found that, with the exception of one CS clone sequence that was shared by one P. vivax isolate each from PNG and Brazil, the P. vivax CS protein types can be grouped into Papuan and Brazilian types. These results suggest that an in-depth study of parasite population dynamics is required before field trials for vaccine formulations based on polymorphic immunodominant determinants are conducted. |
publishDate |
1992 |
dc.date.issued.fl_str_mv |
1992 |
dc.date.accessioned.fl_str_mv |
2017-06-09T12:55:54Z |
dc.date.available.fl_str_mv |
2017-06-09T12:55:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
QARI, Shoukat H. et al. Polymorphism in the circumsporozoite protein of the human malaria parasite Plasmodium vivax. Molecular and Biochemical Parasitology, v. 55, p. 105-114, 1992. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/2593 |
dc.identifier.issn.-.fl_str_mv |
0166-6851 |
identifier_str_mv |
QARI, Shoukat H. et al. Polymorphism in the circumsporozoite protein of the human malaria parasite Plasmodium vivax. Molecular and Biochemical Parasitology, v. 55, p. 105-114, 1992. 0166-6851 |
url |
https://patua.iec.gov.br/handle/iec/2593 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
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IEC |
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IEC |
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collection |
Repositório Digital do Instituto Evandro Chagas (Patuá) |
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