Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys

Detalhes bibliográficos
Autor(a) principal: Alcantara, Bianca Nascimento de
Data de Publicação: 2021
Outros Autores: Imbeloni, Aline Amaral, Durans, Darlene de Brito Simith, Araújo, Marialva Tereza Ferreira de, Cruz, Ermelinda do Rosário Moutinho da, Carvalho, Carlos Alberto Marques de, Mendonça, Maria Helena Rodrigues de, Sousa, Jorge Rodrigues de, Moraes, Adriana Freitas, Martins Filho, Arnaldo Jorge, Lima, Maria de Lourdes Gomes de, Amador Neto, Orlando Pereira, Chiang, Jannifer Oliveira, Scalercio, Sarah Raphaella Rocha de Azevedo, Carneiro, Liliane Almeida, Quaresma, Juarez Antônio Simões, Vasconcelos, Pedro Fernando da Costa, Medeiros, Daniele Barbosa de Almeida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/4271
https://doi.org/10.1038/s41598-021-85571-1
Resumo: The absence of an adequate animal model for studies has limited the understanding of congenital Zika syndrome (CZS) in humans during the outbreak in America. In this study, we used squirrel monkeys (Saimiri collinsi), a neotropical primate (which mimics the stages of human pregnancy), as a model of Zika virus (ZIKV) infection. Seven pregnant female squirrel monkeys were experimentally infected at three different gestational stages, and we were able reproduce a broad range of clinical manifestations of ZIKV lesions observed in newborn humans. Histopathological and immunohistochemical analyses of early-infected newborns (2/4) revealed damage to various areas of the brain and ZIKV antigens in the cytoplasm of neurons and glial cells, indicative of CZS. The changes caused by ZIKV infection were intrauterine developmental delay, ventriculomegaly, simplified brain gyri, vascular impairment and neuroprogenitor cell dysfunction. Our data show that the ZIKV infection outcome in squirrel monkeys is similar to that in humans, indicating that this model can be used to help answer questions about the effect of ZIKV infection on neuroembryonic development and the morphological changes induced by CZS.
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spelling Alcantara, Bianca Nascimento deImbeloni, Aline AmaralDurans, Darlene de Brito SimithAraújo, Marialva Tereza Ferreira deCruz, Ermelinda do Rosário Moutinho daCarvalho, Carlos Alberto Marques deMendonça, Maria Helena Rodrigues deSousa, Jorge Rodrigues deMoraes, Adriana FreitasMartins Filho, Arnaldo JorgeLima, Maria de Lourdes Gomes deAmador Neto, Orlando PereiraChiang, Jannifer OliveiraScalercio, Sarah Raphaella Rocha de AzevedoCarneiro, Liliane AlmeidaQuaresma, Juarez Antônio SimõesVasconcelos, Pedro Fernando da CostaMedeiros, Daniele Barbosa de Almeida2021-03-29T14:01:37Z2021-03-29T14:01:37Z2021ALCANTARA, Bianca Nascimento de et al. Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys. Scientific Reports, v. 11, n. 6099, 2021. DOI: https://doi.org/10.1038/s41598-021-85571-1. Disponível em: https://www.nature.com/articles/s41598-021-85571-1.pdf. Acesso em: 29 mar. 2021.2045-2322https://patua.iec.gov.br/handle/iec/4271https://doi.org/10.1038/s41598-021-85571-1The absence of an adequate animal model for studies has limited the understanding of congenital Zika syndrome (CZS) in humans during the outbreak in America. In this study, we used squirrel monkeys (Saimiri collinsi), a neotropical primate (which mimics the stages of human pregnancy), as a model of Zika virus (ZIKV) infection. Seven pregnant female squirrel monkeys were experimentally infected at three different gestational stages, and we were able reproduce a broad range of clinical manifestations of ZIKV lesions observed in newborn humans. Histopathological and immunohistochemical analyses of early-infected newborns (2/4) revealed damage to various areas of the brain and ZIKV antigens in the cytoplasm of neurons and glial cells, indicative of CZS. The changes caused by ZIKV infection were intrauterine developmental delay, ventriculomegaly, simplified brain gyri, vascular impairment and neuroprogenitor cell dysfunction. Our data show that the ZIKV infection outcome in squirrel monkeys is similar to that in humans, indicating that this model can be used to help answer questions about the effect of ZIKV infection on neuroembryonic development and the morphological changes induced by CZS.This study was supported by grants from CNPq, CAPES (Zika Fast-Track, 440405/2016-5) and FINEP agencies to P.F.C.V. (CNPq: 303999/2016-0) and D.B.A.M. (CNPq: 306581/2016-7).Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Programa de Pós-Graduação em Virologia. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro Nacional de Primatas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Pará State University. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro Nacional de Primatas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro Nacional de Primatas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Pará State University. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.engNature ResearchHistopathological lesions of congenital Zika syndrome in newborn squirrel monkeysinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePrimatas / anatomia & histologiaSaimiri / anatomia & histologiaPrimatas / lesõesInfecção por Zika virus / veterináriaCérebro / lesõesExame Neurológico / veterináriainfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALHistopathological lesions of congenital Zika syndrome in newborn squirrel monkeys.pdfHistopathological lesions of congenital Zika syndrome in newborn squirrel monkeys.pdfapplication/pdf4485769https://patua.iec.gov.br/bitstreams/b3a9ce7f-5765-438e-8ba3-70341738fba7/download1b779ab29e717582eaab55a82759fc3cMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/704e1eac-8bd5-4659-a1b2-c38ebadb6682/download11832eea31b16df8613079d742d61793MD52TEXTHistopathological lesions of congenital Zika syndrome in newborn squirrel monkeys.pdf.txtHistopathological lesions of congenital Zika syndrome in newborn squirrel monkeys.pdf.txtExtracted texttext/plain57720https://patua.iec.gov.br/bitstreams/13fccbfd-fa41-41fe-ad94-4f699a9a9d80/download7972f6b06ecd4e7b60616b94d054aad0MD55THUMBNAILHistopathological lesions of congenital Zika syndrome in newborn squirrel monkeys.pdf.jpgHistopathological lesions of congenital Zika syndrome in newborn squirrel monkeys.pdf.jpgGenerated Thumbnailimage/jpeg6277https://patua.iec.gov.br/bitstreams/19e84057-c839-42d9-be69-2c3ffc2e852b/downloadcab9cbf46736b1ca85ca6ddbed04476cMD56iec/42712022-10-20 22:23:13.644oai:patua.iec.gov.br:iec/4271https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T22:23:13Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)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
dc.title.pt_BR.fl_str_mv Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys
title Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys
spellingShingle Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys
Alcantara, Bianca Nascimento de
Primatas / anatomia & histologia
Saimiri / anatomia & histologia
Primatas / lesões
Infecção por Zika virus / veterinária
Cérebro / lesões
Exame Neurológico / veterinária
title_short Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys
title_full Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys
title_fullStr Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys
title_full_unstemmed Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys
title_sort Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys
author Alcantara, Bianca Nascimento de
author_facet Alcantara, Bianca Nascimento de
Imbeloni, Aline Amaral
Durans, Darlene de Brito Simith
Araújo, Marialva Tereza Ferreira de
Cruz, Ermelinda do Rosário Moutinho da
Carvalho, Carlos Alberto Marques de
Mendonça, Maria Helena Rodrigues de
Sousa, Jorge Rodrigues de
Moraes, Adriana Freitas
Martins Filho, Arnaldo Jorge
Lima, Maria de Lourdes Gomes de
Amador Neto, Orlando Pereira
Chiang, Jannifer Oliveira
Scalercio, Sarah Raphaella Rocha de Azevedo
Carneiro, Liliane Almeida
Quaresma, Juarez Antônio Simões
Vasconcelos, Pedro Fernando da Costa
Medeiros, Daniele Barbosa de Almeida
author_role author
author2 Imbeloni, Aline Amaral
Durans, Darlene de Brito Simith
Araújo, Marialva Tereza Ferreira de
Cruz, Ermelinda do Rosário Moutinho da
Carvalho, Carlos Alberto Marques de
Mendonça, Maria Helena Rodrigues de
Sousa, Jorge Rodrigues de
Moraes, Adriana Freitas
Martins Filho, Arnaldo Jorge
Lima, Maria de Lourdes Gomes de
Amador Neto, Orlando Pereira
Chiang, Jannifer Oliveira
Scalercio, Sarah Raphaella Rocha de Azevedo
Carneiro, Liliane Almeida
Quaresma, Juarez Antônio Simões
Vasconcelos, Pedro Fernando da Costa
Medeiros, Daniele Barbosa de Almeida
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Alcantara, Bianca Nascimento de
Imbeloni, Aline Amaral
Durans, Darlene de Brito Simith
Araújo, Marialva Tereza Ferreira de
Cruz, Ermelinda do Rosário Moutinho da
Carvalho, Carlos Alberto Marques de
Mendonça, Maria Helena Rodrigues de
Sousa, Jorge Rodrigues de
Moraes, Adriana Freitas
Martins Filho, Arnaldo Jorge
Lima, Maria de Lourdes Gomes de
Amador Neto, Orlando Pereira
Chiang, Jannifer Oliveira
Scalercio, Sarah Raphaella Rocha de Azevedo
Carneiro, Liliane Almeida
Quaresma, Juarez Antônio Simões
Vasconcelos, Pedro Fernando da Costa
Medeiros, Daniele Barbosa de Almeida
dc.subject.decsPrimary.pt_BR.fl_str_mv Primatas / anatomia & histologia
Saimiri / anatomia & histologia
Primatas / lesões
Infecção por Zika virus / veterinária
Cérebro / lesões
Exame Neurológico / veterinária
topic Primatas / anatomia & histologia
Saimiri / anatomia & histologia
Primatas / lesões
Infecção por Zika virus / veterinária
Cérebro / lesões
Exame Neurológico / veterinária
description The absence of an adequate animal model for studies has limited the understanding of congenital Zika syndrome (CZS) in humans during the outbreak in America. In this study, we used squirrel monkeys (Saimiri collinsi), a neotropical primate (which mimics the stages of human pregnancy), as a model of Zika virus (ZIKV) infection. Seven pregnant female squirrel monkeys were experimentally infected at three different gestational stages, and we were able reproduce a broad range of clinical manifestations of ZIKV lesions observed in newborn humans. Histopathological and immunohistochemical analyses of early-infected newborns (2/4) revealed damage to various areas of the brain and ZIKV antigens in the cytoplasm of neurons and glial cells, indicative of CZS. The changes caused by ZIKV infection were intrauterine developmental delay, ventriculomegaly, simplified brain gyri, vascular impairment and neuroprogenitor cell dysfunction. Our data show that the ZIKV infection outcome in squirrel monkeys is similar to that in humans, indicating that this model can be used to help answer questions about the effect of ZIKV infection on neuroembryonic development and the morphological changes induced by CZS.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-03-29T14:01:37Z
dc.date.available.fl_str_mv 2021-03-29T14:01:37Z
dc.date.issued.fl_str_mv 2021
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status_str publishedVersion
dc.identifier.citation.fl_str_mv ALCANTARA, Bianca Nascimento de et al. Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys. Scientific Reports, v. 11, n. 6099, 2021. DOI: https://doi.org/10.1038/s41598-021-85571-1. Disponível em: https://www.nature.com/articles/s41598-021-85571-1.pdf. Acesso em: 29 mar. 2021.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/4271
dc.identifier.issn.-.fl_str_mv 2045-2322
dc.identifier.doi.-.fl_str_mv https://doi.org/10.1038/s41598-021-85571-1
identifier_str_mv ALCANTARA, Bianca Nascimento de et al. Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys. Scientific Reports, v. 11, n. 6099, 2021. DOI: https://doi.org/10.1038/s41598-021-85571-1. Disponível em: https://www.nature.com/articles/s41598-021-85571-1.pdf. Acesso em: 29 mar. 2021.
2045-2322
url https://patua.iec.gov.br/handle/iec/4271
https://doi.org/10.1038/s41598-021-85571-1
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