Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/4271 https://doi.org/10.1038/s41598-021-85571-1 |
Resumo: | The absence of an adequate animal model for studies has limited the understanding of congenital Zika syndrome (CZS) in humans during the outbreak in America. In this study, we used squirrel monkeys (Saimiri collinsi), a neotropical primate (which mimics the stages of human pregnancy), as a model of Zika virus (ZIKV) infection. Seven pregnant female squirrel monkeys were experimentally infected at three different gestational stages, and we were able reproduce a broad range of clinical manifestations of ZIKV lesions observed in newborn humans. Histopathological and immunohistochemical analyses of early-infected newborns (2/4) revealed damage to various areas of the brain and ZIKV antigens in the cytoplasm of neurons and glial cells, indicative of CZS. The changes caused by ZIKV infection were intrauterine developmental delay, ventriculomegaly, simplified brain gyri, vascular impairment and neuroprogenitor cell dysfunction. Our data show that the ZIKV infection outcome in squirrel monkeys is similar to that in humans, indicating that this model can be used to help answer questions about the effect of ZIKV infection on neuroembryonic development and the morphological changes induced by CZS. |
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Alcantara, Bianca Nascimento deImbeloni, Aline AmaralDurans, Darlene de Brito SimithAraújo, Marialva Tereza Ferreira deCruz, Ermelinda do Rosário Moutinho daCarvalho, Carlos Alberto Marques deMendonça, Maria Helena Rodrigues deSousa, Jorge Rodrigues deMoraes, Adriana FreitasMartins Filho, Arnaldo JorgeLima, Maria de Lourdes Gomes deAmador Neto, Orlando PereiraChiang, Jannifer OliveiraScalercio, Sarah Raphaella Rocha de AzevedoCarneiro, Liliane AlmeidaQuaresma, Juarez Antônio SimõesVasconcelos, Pedro Fernando da CostaMedeiros, Daniele Barbosa de Almeida2021-03-29T14:01:37Z2021-03-29T14:01:37Z2021ALCANTARA, Bianca Nascimento de et al. Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys. Scientific Reports, v. 11, n. 6099, 2021. DOI: https://doi.org/10.1038/s41598-021-85571-1. Disponível em: https://www.nature.com/articles/s41598-021-85571-1.pdf. Acesso em: 29 mar. 2021.2045-2322https://patua.iec.gov.br/handle/iec/4271https://doi.org/10.1038/s41598-021-85571-1The absence of an adequate animal model for studies has limited the understanding of congenital Zika syndrome (CZS) in humans during the outbreak in America. In this study, we used squirrel monkeys (Saimiri collinsi), a neotropical primate (which mimics the stages of human pregnancy), as a model of Zika virus (ZIKV) infection. Seven pregnant female squirrel monkeys were experimentally infected at three different gestational stages, and we were able reproduce a broad range of clinical manifestations of ZIKV lesions observed in newborn humans. Histopathological and immunohistochemical analyses of early-infected newborns (2/4) revealed damage to various areas of the brain and ZIKV antigens in the cytoplasm of neurons and glial cells, indicative of CZS. The changes caused by ZIKV infection were intrauterine developmental delay, ventriculomegaly, simplified brain gyri, vascular impairment and neuroprogenitor cell dysfunction. Our data show that the ZIKV infection outcome in squirrel monkeys is similar to that in humans, indicating that this model can be used to help answer questions about the effect of ZIKV infection on neuroembryonic development and the morphological changes induced by CZS.This study was supported by grants from CNPq, CAPES (Zika Fast-Track, 440405/2016-5) and FINEP agencies to P.F.C.V. (CNPq: 303999/2016-0) and D.B.A.M. (CNPq: 306581/2016-7).Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Programa de Pós-Graduação em Virologia. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro Nacional de Primatas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Pará State University. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro Nacional de Primatas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro Nacional de Primatas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Pará State University. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.engNature ResearchHistopathological lesions of congenital Zika syndrome in newborn squirrel monkeysinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePrimatas / anatomia & histologiaSaimiri / anatomia & histologiaPrimatas / lesõesInfecção por Zika virus / veterináriaCérebro / lesõesExame Neurológico / veterináriainfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALHistopathological lesions of congenital Zika syndrome in newborn squirrel monkeys.pdfHistopathological lesions of congenital Zika syndrome in newborn squirrel monkeys.pdfapplication/pdf4485769https://patua.iec.gov.br/bitstreams/b3a9ce7f-5765-438e-8ba3-70341738fba7/download1b779ab29e717582eaab55a82759fc3cMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/704e1eac-8bd5-4659-a1b2-c38ebadb6682/download11832eea31b16df8613079d742d61793MD52TEXTHistopathological lesions of congenital Zika syndrome in newborn squirrel monkeys.pdf.txtHistopathological lesions of congenital Zika syndrome in newborn squirrel monkeys.pdf.txtExtracted texttext/plain57720https://patua.iec.gov.br/bitstreams/13fccbfd-fa41-41fe-ad94-4f699a9a9d80/download7972f6b06ecd4e7b60616b94d054aad0MD55THUMBNAILHistopathological lesions of congenital Zika syndrome in newborn squirrel monkeys.pdf.jpgHistopathological lesions of congenital Zika syndrome in newborn squirrel monkeys.pdf.jpgGenerated Thumbnailimage/jpeg6277https://patua.iec.gov.br/bitstreams/19e84057-c839-42d9-be69-2c3ffc2e852b/downloadcab9cbf46736b1ca85ca6ddbed04476cMD56iec/42712022-10-20 22:23:13.644oai:patua.iec.gov.br:iec/4271https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T22:23:13Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)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 |
dc.title.pt_BR.fl_str_mv |
Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys |
title |
Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys |
spellingShingle |
Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys Alcantara, Bianca Nascimento de Primatas / anatomia & histologia Saimiri / anatomia & histologia Primatas / lesões Infecção por Zika virus / veterinária Cérebro / lesões Exame Neurológico / veterinária |
title_short |
Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys |
title_full |
Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys |
title_fullStr |
Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys |
title_full_unstemmed |
Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys |
title_sort |
Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys |
author |
Alcantara, Bianca Nascimento de |
author_facet |
Alcantara, Bianca Nascimento de Imbeloni, Aline Amaral Durans, Darlene de Brito Simith Araújo, Marialva Tereza Ferreira de Cruz, Ermelinda do Rosário Moutinho da Carvalho, Carlos Alberto Marques de Mendonça, Maria Helena Rodrigues de Sousa, Jorge Rodrigues de Moraes, Adriana Freitas Martins Filho, Arnaldo Jorge Lima, Maria de Lourdes Gomes de Amador Neto, Orlando Pereira Chiang, Jannifer Oliveira Scalercio, Sarah Raphaella Rocha de Azevedo Carneiro, Liliane Almeida Quaresma, Juarez Antônio Simões Vasconcelos, Pedro Fernando da Costa Medeiros, Daniele Barbosa de Almeida |
author_role |
author |
author2 |
Imbeloni, Aline Amaral Durans, Darlene de Brito Simith Araújo, Marialva Tereza Ferreira de Cruz, Ermelinda do Rosário Moutinho da Carvalho, Carlos Alberto Marques de Mendonça, Maria Helena Rodrigues de Sousa, Jorge Rodrigues de Moraes, Adriana Freitas Martins Filho, Arnaldo Jorge Lima, Maria de Lourdes Gomes de Amador Neto, Orlando Pereira Chiang, Jannifer Oliveira Scalercio, Sarah Raphaella Rocha de Azevedo Carneiro, Liliane Almeida Quaresma, Juarez Antônio Simões Vasconcelos, Pedro Fernando da Costa Medeiros, Daniele Barbosa de Almeida |
author2_role |
author author author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Alcantara, Bianca Nascimento de Imbeloni, Aline Amaral Durans, Darlene de Brito Simith Araújo, Marialva Tereza Ferreira de Cruz, Ermelinda do Rosário Moutinho da Carvalho, Carlos Alberto Marques de Mendonça, Maria Helena Rodrigues de Sousa, Jorge Rodrigues de Moraes, Adriana Freitas Martins Filho, Arnaldo Jorge Lima, Maria de Lourdes Gomes de Amador Neto, Orlando Pereira Chiang, Jannifer Oliveira Scalercio, Sarah Raphaella Rocha de Azevedo Carneiro, Liliane Almeida Quaresma, Juarez Antônio Simões Vasconcelos, Pedro Fernando da Costa Medeiros, Daniele Barbosa de Almeida |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Primatas / anatomia & histologia Saimiri / anatomia & histologia Primatas / lesões Infecção por Zika virus / veterinária Cérebro / lesões Exame Neurológico / veterinária |
topic |
Primatas / anatomia & histologia Saimiri / anatomia & histologia Primatas / lesões Infecção por Zika virus / veterinária Cérebro / lesões Exame Neurológico / veterinária |
description |
The absence of an adequate animal model for studies has limited the understanding of congenital Zika syndrome (CZS) in humans during the outbreak in America. In this study, we used squirrel monkeys (Saimiri collinsi), a neotropical primate (which mimics the stages of human pregnancy), as a model of Zika virus (ZIKV) infection. Seven pregnant female squirrel monkeys were experimentally infected at three different gestational stages, and we were able reproduce a broad range of clinical manifestations of ZIKV lesions observed in newborn humans. Histopathological and immunohistochemical analyses of early-infected newborns (2/4) revealed damage to various areas of the brain and ZIKV antigens in the cytoplasm of neurons and glial cells, indicative of CZS. The changes caused by ZIKV infection were intrauterine developmental delay, ventriculomegaly, simplified brain gyri, vascular impairment and neuroprogenitor cell dysfunction. Our data show that the ZIKV infection outcome in squirrel monkeys is similar to that in humans, indicating that this model can be used to help answer questions about the effect of ZIKV infection on neuroembryonic development and the morphological changes induced by CZS. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-03-29T14:01:37Z |
dc.date.available.fl_str_mv |
2021-03-29T14:01:37Z |
dc.date.issued.fl_str_mv |
2021 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ALCANTARA, Bianca Nascimento de et al. Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys. Scientific Reports, v. 11, n. 6099, 2021. DOI: https://doi.org/10.1038/s41598-021-85571-1. Disponível em: https://www.nature.com/articles/s41598-021-85571-1.pdf. Acesso em: 29 mar. 2021. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/4271 |
dc.identifier.issn.-.fl_str_mv |
2045-2322 |
dc.identifier.doi.-.fl_str_mv |
https://doi.org/10.1038/s41598-021-85571-1 |
identifier_str_mv |
ALCANTARA, Bianca Nascimento de et al. Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys. Scientific Reports, v. 11, n. 6099, 2021. DOI: https://doi.org/10.1038/s41598-021-85571-1. Disponível em: https://www.nature.com/articles/s41598-021-85571-1.pdf. Acesso em: 29 mar. 2021. 2045-2322 |
url |
https://patua.iec.gov.br/handle/iec/4271 https://doi.org/10.1038/s41598-021-85571-1 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Nature Research |
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Nature Research |
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