Neurological infection by chikungunya and a triple Arbovirus co-infection in Mato Grosso, Central Western Brazil during 2019
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/4547 |
Resumo: | Background: Neurological viral infection is frequently associated to enterovirus, herpesvirus and arboviruses. These infections may cause severe clinical outcomes, long lasting sequelae or death. Few studies have addressed viral neurological infections etiology in Brazil. Objectives: Identification of viruses in the cerebral spinal fluid (CSF) of human neurological infections suspected of viral etiology during January and May 2019 in Midwestern Brazil. Materials and methods: Clinical, laboratory and epidemiological information was gathered from medical records. In addition, an aliquot of the sampled CSF was subjected to viral RNA/DNA extraction, randomic dscDNA amplification by PCR, DNA purification and Ilumina HiSeq 2500 sequencing. Results: Six viral genomes belonging to Chikungunya virus (CHIKV) East-Central-South African (ECSA) genotype (10.834-11.804 nt in length) confirmed lately by RT-PCR for CHIKV envelope were present in all six liquor samples. These genomes present two mutations, nsP2:T31I and nsP3:A388V, shared with other Mato Grosso State strains from 2019, not present in sequences of the virus from previous years obtained in the State. One case was a triple co-infection also confirmed through RT-PCR, with Dengue virus serotype 4 genotype II (NS5; 874 nt) and Oropouche virus genotype IA (segment S; 302 nt). CSF was clear and colorless (5/6 patients), with >10% of lymphomononuclear cells (6/6), 1-99 erythrocytes/mm3 (5/6), glucose levels >50 mg/dl (4/5) e > 10 mg/dl of proteins (4/4). One patient evolved to death, and another, a newborn, presented sequelae after recovery. Conclusions: Despite herpesviruses and enteroviruses are frequent etiologies of neurological infections, the casuistic here reported was associated to arboviruses already known to be responsible for acute febrile illness outbreaks in the state of Mato Grosso, Midwestern Brazil. |
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Pavon, Janeth Aracely RamirezNeves, Nilvanei Aparecido da SilvaSilva, Ludmilla Campos FernandesAzevedo, Francisco Kennedy deFigueiredo Junior, José Alexander B. deNunes, Márcio Roberto TeixeiraSlhessarenko, Renata Dezengrini2022-06-03T14:40:57Z2022-06-03T14:40:57Z2022PAVON, Janeth Aracely Ramirez et al. Neurological infection by chikungunya and a triple Arbovirus co-infection in Mato Grosso, Central Western Brazil during 2019. Journal of Clinical Virology, v. 146, n. 105056, p. xx, Jan. 2022.1386-6532https://patua.iec.gov.br/handle/iec/454710.1016/j.jcv.2021.105056Background: Neurological viral infection is frequently associated to enterovirus, herpesvirus and arboviruses. These infections may cause severe clinical outcomes, long lasting sequelae or death. Few studies have addressed viral neurological infections etiology in Brazil. Objectives: Identification of viruses in the cerebral spinal fluid (CSF) of human neurological infections suspected of viral etiology during January and May 2019 in Midwestern Brazil. Materials and methods: Clinical, laboratory and epidemiological information was gathered from medical records. In addition, an aliquot of the sampled CSF was subjected to viral RNA/DNA extraction, randomic dscDNA amplification by PCR, DNA purification and Ilumina HiSeq 2500 sequencing. Results: Six viral genomes belonging to Chikungunya virus (CHIKV) East-Central-South African (ECSA) genotype (10.834-11.804 nt in length) confirmed lately by RT-PCR for CHIKV envelope were present in all six liquor samples. These genomes present two mutations, nsP2:T31I and nsP3:A388V, shared with other Mato Grosso State strains from 2019, not present in sequences of the virus from previous years obtained in the State. One case was a triple co-infection also confirmed through RT-PCR, with Dengue virus serotype 4 genotype II (NS5; 874 nt) and Oropouche virus genotype IA (segment S; 302 nt). CSF was clear and colorless (5/6 patients), with >10% of lymphomononuclear cells (6/6), 1-99 erythrocytes/mm3 (5/6), glucose levels >50 mg/dl (4/5) e > 10 mg/dl of proteins (4/4). One patient evolved to death, and another, a newborn, presented sequelae after recovery. Conclusions: Despite herpesviruses and enteroviruses are frequent etiologies of neurological infections, the casuistic here reported was associated to arboviruses already known to be responsible for acute febrile illness outbreaks in the state of Mato Grosso, Midwestern Brazil.Universidade Federal de Mato Grosso - Campus Cuiabá. Faculdade de Medicina. Departamento de Ciências Básicas em Saúde. Programa de Pós-graduação em Ciências da Saúde. Cuiabá, MT, BrazilUniversidade Federal de Mato Grosso - Campus Cuiabá. Faculdade de Medicina. Departamento de Ciências Básicas em Saúde. Programa de Pós-graduação em Ciências da Saúde. Cuiabá, MT, BrazilUniversidade Federal de Mato Grosso - Campus Cuiabá. Faculdade de Medicina. Departamento de Ciências Básicas em Saúde. Programa de Pós-graduação em Ciências da Saúde. Cuiabá, MT, BrazilUniversidade Federal de Mato Grosso - Campus Cuiabá. Faculdade de Medicina. Departamento de Ciências Básicas em Saúde. Programa de Pós-graduação em Ciências da Saúde. Cuiabá, MT, Brazil / Hospital Universitário Júlio Muller. Cuiabá, MT, BrazilHospital Universitário Júlio Muller. Cuiabá, MT, BrazilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilUniversidade Federal de Mato Grosso, Campus Cuiabá. Faculdade de Medicina. Departamento de Ciências Básicas em Saúde. Programa de Pós-graduação em Ciências da Saúde. Cuiabá, MT, BrazilengElsevierNeurological infection by chikungunya and a triple Arbovirus co-infection in Mato Grosso, Central Western Brazil during 2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleVírus Chikungunya / patogenicidadeFebre de Chikungunya / líquido cefalorraquidianoinfecções neurológicasinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALNeurological infection by chikungunya and a triple Arbovirus co-infection in Mato Grosso, Central Western Brazil during 2019.pdfNeurological infection by chikungunya and a triple Arbovirus co-infection in Mato Grosso, Central Western Brazil during 2019.pdfapplication/pdf551083https://patua.iec.gov.br/bitstreams/34609569-8dc9-4197-b0ff-4a6c9a144ac8/downloadc9a9c128e29cac82a5d7fdf3f4e6da73MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/ed6e722e-f8b0-4049-8aed-67d1f00168c5/download11832eea31b16df8613079d742d61793MD52TEXTNeurological infection by chikungunya and a triple Arbovirus co-infection in Mato Grosso, Central Western Brazil during 2019.pdf.txtNeurological infection by chikungunya and a triple Arbovirus co-infection in Mato Grosso, Central Western Brazil during 2019.pdf.txtExtracted texttext/plain2https://patua.iec.gov.br/bitstreams/77b9d5e7-1575-4917-8522-63e8512d5f94/downloade1c06d85ae7b8b032bef47e42e4c08f9MD55THUMBNAILNeurological infection by chikungunya and a triple Arbovirus co-infection in Mato Grosso, Central Western Brazil during 2019.pdf.jpgNeurological infection by chikungunya and a triple Arbovirus co-infection in Mato Grosso, Central Western Brazil during 2019.pdf.jpgGenerated Thumbnailimage/jpeg3095https://patua.iec.gov.br/bitstreams/6a2038f1-763a-4ec8-9e2b-589446e93dff/download71859d578212107f7f8c49a4ce09d9eeMD56iec/45472022-10-20 22:40:46.981oai:patua.iec.gov.br:iec/4547https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T22:40:46Repositório Digital do Instituto Evandro Chagas (Patuá) - 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dc.title.pt_BR.fl_str_mv |
Neurological infection by chikungunya and a triple Arbovirus co-infection in Mato Grosso, Central Western Brazil during 2019 |
title |
Neurological infection by chikungunya and a triple Arbovirus co-infection in Mato Grosso, Central Western Brazil during 2019 |
spellingShingle |
Neurological infection by chikungunya and a triple Arbovirus co-infection in Mato Grosso, Central Western Brazil during 2019 Pavon, Janeth Aracely Ramirez Vírus Chikungunya / patogenicidade Febre de Chikungunya / líquido cefalorraquidiano infecções neurológicas |
title_short |
Neurological infection by chikungunya and a triple Arbovirus co-infection in Mato Grosso, Central Western Brazil during 2019 |
title_full |
Neurological infection by chikungunya and a triple Arbovirus co-infection in Mato Grosso, Central Western Brazil during 2019 |
title_fullStr |
Neurological infection by chikungunya and a triple Arbovirus co-infection in Mato Grosso, Central Western Brazil during 2019 |
title_full_unstemmed |
Neurological infection by chikungunya and a triple Arbovirus co-infection in Mato Grosso, Central Western Brazil during 2019 |
title_sort |
Neurological infection by chikungunya and a triple Arbovirus co-infection in Mato Grosso, Central Western Brazil during 2019 |
author |
Pavon, Janeth Aracely Ramirez |
author_facet |
Pavon, Janeth Aracely Ramirez Neves, Nilvanei Aparecido da Silva Silva, Ludmilla Campos Fernandes Azevedo, Francisco Kennedy de Figueiredo Junior, José Alexander B. de Nunes, Márcio Roberto Teixeira Slhessarenko, Renata Dezengrini |
author_role |
author |
author2 |
Neves, Nilvanei Aparecido da Silva Silva, Ludmilla Campos Fernandes Azevedo, Francisco Kennedy de Figueiredo Junior, José Alexander B. de Nunes, Márcio Roberto Teixeira Slhessarenko, Renata Dezengrini |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Pavon, Janeth Aracely Ramirez Neves, Nilvanei Aparecido da Silva Silva, Ludmilla Campos Fernandes Azevedo, Francisco Kennedy de Figueiredo Junior, José Alexander B. de Nunes, Márcio Roberto Teixeira Slhessarenko, Renata Dezengrini |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Vírus Chikungunya / patogenicidade Febre de Chikungunya / líquido cefalorraquidiano infecções neurológicas |
topic |
Vírus Chikungunya / patogenicidade Febre de Chikungunya / líquido cefalorraquidiano infecções neurológicas |
description |
Background: Neurological viral infection is frequently associated to enterovirus, herpesvirus and arboviruses. These infections may cause severe clinical outcomes, long lasting sequelae or death. Few studies have addressed viral neurological infections etiology in Brazil. Objectives: Identification of viruses in the cerebral spinal fluid (CSF) of human neurological infections suspected of viral etiology during January and May 2019 in Midwestern Brazil. Materials and methods: Clinical, laboratory and epidemiological information was gathered from medical records. In addition, an aliquot of the sampled CSF was subjected to viral RNA/DNA extraction, randomic dscDNA amplification by PCR, DNA purification and Ilumina HiSeq 2500 sequencing. Results: Six viral genomes belonging to Chikungunya virus (CHIKV) East-Central-South African (ECSA) genotype (10.834-11.804 nt in length) confirmed lately by RT-PCR for CHIKV envelope were present in all six liquor samples. These genomes present two mutations, nsP2:T31I and nsP3:A388V, shared with other Mato Grosso State strains from 2019, not present in sequences of the virus from previous years obtained in the State. One case was a triple co-infection also confirmed through RT-PCR, with Dengue virus serotype 4 genotype II (NS5; 874 nt) and Oropouche virus genotype IA (segment S; 302 nt). CSF was clear and colorless (5/6 patients), with >10% of lymphomononuclear cells (6/6), 1-99 erythrocytes/mm3 (5/6), glucose levels >50 mg/dl (4/5) e > 10 mg/dl of proteins (4/4). One patient evolved to death, and another, a newborn, presented sequelae after recovery. Conclusions: Despite herpesviruses and enteroviruses are frequent etiologies of neurological infections, the casuistic here reported was associated to arboviruses already known to be responsible for acute febrile illness outbreaks in the state of Mato Grosso, Midwestern Brazil. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-06-03T14:40:57Z |
dc.date.available.fl_str_mv |
2022-06-03T14:40:57Z |
dc.date.issued.fl_str_mv |
2022 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
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article |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
PAVON, Janeth Aracely Ramirez et al. Neurological infection by chikungunya and a triple Arbovirus co-infection in Mato Grosso, Central Western Brazil during 2019. Journal of Clinical Virology, v. 146, n. 105056, p. xx, Jan. 2022. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/4547 |
dc.identifier.issn.-.fl_str_mv |
1386-6532 |
dc.identifier.doi.-.fl_str_mv |
10.1016/j.jcv.2021.105056 |
identifier_str_mv |
PAVON, Janeth Aracely Ramirez et al. Neurological infection by chikungunya and a triple Arbovirus co-infection in Mato Grosso, Central Western Brazil during 2019. Journal of Clinical Virology, v. 146, n. 105056, p. xx, Jan. 2022. 1386-6532 10.1016/j.jcv.2021.105056 |
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https://patua.iec.gov.br/handle/iec/4547 |
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eng |
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eng |
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Elsevier |
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Elsevier |
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