Distinct evolutionary origins of G12P[8] and G12P[9] group A rotavirus strains circulating in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/981 |
Resumo: | G12 group A rotavirus (RVA) are currently recognized as a globally emerging genotype and have been described in combination with several P-types. In Brazil, G12 RVA strains have been described in the Southern (2003) and Northern (2008 2010) regions, in combination with the P[9] and P[6] genotype, respectively. To date, few complete genomes of G12 RVA strains have been described (none from Brazilian strains), considering G12P[9] genotype just one strain, RVA/Human-tc/THA/T152/1998/G12P[9], has their 11 gene segments characterized. This study aims to determine the genomic constellation of G12P[9] and G12P[8] RVA strains detected in Brazil between 2006 and 2011. Therefore, the eleven gene segments of five Brazilian G12 RVA strains were amplified and sequenced, and the genotype of each gene segment was assigned using phylogenetic analysis. Complete genome analyses of G12 RVA strain circulating between 2006 and 2011 in Brazil revealed a conserved Wa-like genomic constellation for three G12P[8] RVA strains; whereas the two G12P[9] strains possessed distinct reassorted AU-1-like genomic constellations, closely related to the reference strain RVA/Human-tc/THA/T152/1998/G12P [9] in most genes. The results obtained in the current study suggest that G12P [9] (AU-1-like) and G12P[8] (Wa-like) strains detected in different regions of Brazil do not share a common origin. Moreover, while Brazilian G12P[8] RVA strains showed a complete Wa-like human constellation, both G12P[9] strains possessed an NSP1 gene of bovine origin (NSP1), and RVA/Human-wt/BRA/PE18974/2010/G12P[9] also possessed a VP3 gene of canine/feline origin. |
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Gómez, Mariela MResque, Hugo ReisVolotão, Eduardo de MelloRose, Tatiana LundgrenSilva, Marcelle Figueira Marques daHeylen, ElisabethZeller, MarkMatthijnssens, JelleLeite, José Paulo Gagliardi2016-01-26T11:39:53Z2016-01-26T11:39:53Z2014GÓMEZ, Mariela M. et al. Distinct evolutionary origins of G12P[8] and G12P[9] group A rotavirus strains circulating in Brazil. Infection Genetics Evolution, v. 28, p. 385-388, 2014.1567-7257https://patua.iec.gov.br/handle/iec/98110.1016/j.meegid.2014.04.007G12 group A rotavirus (RVA) are currently recognized as a globally emerging genotype and have been described in combination with several P-types. In Brazil, G12 RVA strains have been described in the Southern (2003) and Northern (2008 2010) regions, in combination with the P[9] and P[6] genotype, respectively. To date, few complete genomes of G12 RVA strains have been described (none from Brazilian strains), considering G12P[9] genotype just one strain, RVA/Human-tc/THA/T152/1998/G12P[9], has their 11 gene segments characterized. This study aims to determine the genomic constellation of G12P[9] and G12P[8] RVA strains detected in Brazil between 2006 and 2011. Therefore, the eleven gene segments of five Brazilian G12 RVA strains were amplified and sequenced, and the genotype of each gene segment was assigned using phylogenetic analysis. Complete genome analyses of G12 RVA strain circulating between 2006 and 2011 in Brazil revealed a conserved Wa-like genomic constellation for three G12P[8] RVA strains; whereas the two G12P[9] strains possessed distinct reassorted AU-1-like genomic constellations, closely related to the reference strain RVA/Human-tc/THA/T152/1998/G12P [9] in most genes. The results obtained in the current study suggest that G12P [9] (AU-1-like) and G12P[8] (Wa-like) strains detected in different regions of Brazil do not share a common origin. Moreover, while Brazilian G12P[8] RVA strains showed a complete Wa-like human constellation, both G12P[9] strains possessed an NSP1 gene of bovine origin (NSP1), and RVA/Human-wt/BRA/PE18974/2010/G12P[9] also possessed a VP3 gene of canine/feline origin.Program of Research Excellence (PROEP – IOC/Fiocruz/CNPq), the National Council for Scientific and Technological Development (CNPq), project PAPES VI/FIOCRUZ – CNPq, Oswaldo Cruz Institute (IOC/FIOCRUZ), Brazilian Federal Agency for Support and Evaluation of Graduate Education (CAPES) – project CAPES-MERCOSUL PPCP 023/2011, the General Coordination of Public Health Laboratories – Secretary of Health Surveillance (CGLAB/SVS), and Carlos Chagas Filho Foundation for Research Support of Rio de Janeiro State (FAPERJ). M.Z. was supported by the Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT Vlaanderen).Oswaldo Cruz Foundation. Oswaldo Cruz Institute. Laboratory of Comparative and Environmental Virology. Rio de Janeiro, RJ, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Oswaldo Cruz Foundation. Oswaldo Cruz Institute. Laboratory of Comparative and Environmental Virology. Rio de Janeiro, RJ, Brazil.Oswaldo Cruz Foundation. Oswaldo Cruz Institute. Laboratory of Comparative and Environmental Virology. Rio de Janeiro, RJ, Brazil.Oswaldo Cruz Foundation. Oswaldo Cruz Institute. Laboratory of Comparative and Environmental Virology. Rio de Janeiro, RJ, Brazil.University of Leuven. Rega Institute for Medical Research. Department of Microbiology and Immunology. Laboratory of Clinical and Epidemiological. Leuven, Belgium.University of Leuven. Rega Institute for Medical Research. Department of Microbiology and Immunology. Laboratory of Clinical and Epidemiological. Leuven, Belgium.University of Leuven. Rega Institute for Medical Research. Department of Microbiology and Immunology. Laboratory of Clinical and Epidemiological. Leuven, Belgium.Oswaldo Cruz Foundation. Oswaldo Cruz Institute. Laboratory of Comparative and Environmental Virology. Rio de Janeiro, RJ, Brazil.application/pdfengElsevierDistinct evolutionary origins of G12P[8] and G12P[9] group A rotavirus strains circulating in Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRotavirus / genéticaGenoma Viralinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALDistinct evolutionary origins of G12P[8] and G12P[9] group A rotavirus strains circulating in Brazil .pdfapplication/pdf1146804https://patua.iec.gov.br/bitstreams/bf1244f8-66e4-496f-984c-51debadf172f/download8530da0db506d1ed2a13b1360523a10cMD51TEXTfile_1.pdf.txtfile_1.pdf.txtExtracted texttext/plain19562https://patua.iec.gov.br/bitstreams/3326e294-bf34-4d9a-b211-5b56282678aa/download12299c78033d9db9108356a00a6cad89MD52Distinct evolutionary origins of G12P[8] and G12P[9] group A rotavirus strains circulating in Brazil .pdf.txtDistinct evolutionary origins of G12P[8] and G12P[9] group A rotavirus strains circulating in Brazil .pdf.txtExtracted texttext/plain23531https://patua.iec.gov.br/bitstreams/25fc32f5-0522-4667-90af-bc11363f23cb/download58e6941f71d3d2c3b0d00b1920ab8ccbMD56THUMBNAILDistinct evolutionary origins of G12P[8] and G12P[9] group A rotavirus strains circulating in Brazil .pdf.jpgDistinct evolutionary origins of G12P[8] and G12P[9] group A rotavirus strains circulating in Brazil .pdf.jpgGenerated Thumbnailimage/jpeg5978https://patua.iec.gov.br/bitstreams/69e7c232-a418-4550-aa86-6e5c91e2c7c4/download5756fc6d8dd3b2a0039f4c94df80562bMD57LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/0a91f55a-7d43-480d-861f-549ea7c67cf6/download11832eea31b16df8613079d742d61793MD55iec/9812022-10-20 21:29:05.19oai:patua.iec.gov.br:iec/981https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T21:29:05Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)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 |
dc.title.pt_BR.fl_str_mv |
Distinct evolutionary origins of G12P[8] and G12P[9] group A rotavirus strains circulating in Brazil |
title |
Distinct evolutionary origins of G12P[8] and G12P[9] group A rotavirus strains circulating in Brazil |
spellingShingle |
Distinct evolutionary origins of G12P[8] and G12P[9] group A rotavirus strains circulating in Brazil Gómez, Mariela M Rotavirus / genética Genoma Viral |
title_short |
Distinct evolutionary origins of G12P[8] and G12P[9] group A rotavirus strains circulating in Brazil |
title_full |
Distinct evolutionary origins of G12P[8] and G12P[9] group A rotavirus strains circulating in Brazil |
title_fullStr |
Distinct evolutionary origins of G12P[8] and G12P[9] group A rotavirus strains circulating in Brazil |
title_full_unstemmed |
Distinct evolutionary origins of G12P[8] and G12P[9] group A rotavirus strains circulating in Brazil |
title_sort |
Distinct evolutionary origins of G12P[8] and G12P[9] group A rotavirus strains circulating in Brazil |
author |
Gómez, Mariela M |
author_facet |
Gómez, Mariela M Resque, Hugo Reis Volotão, Eduardo de Mello Rose, Tatiana Lundgren Silva, Marcelle Figueira Marques da Heylen, Elisabeth Zeller, Mark Matthijnssens, Jelle Leite, José Paulo Gagliardi |
author_role |
author |
author2 |
Resque, Hugo Reis Volotão, Eduardo de Mello Rose, Tatiana Lundgren Silva, Marcelle Figueira Marques da Heylen, Elisabeth Zeller, Mark Matthijnssens, Jelle Leite, José Paulo Gagliardi |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Gómez, Mariela M Resque, Hugo Reis Volotão, Eduardo de Mello Rose, Tatiana Lundgren Silva, Marcelle Figueira Marques da Heylen, Elisabeth Zeller, Mark Matthijnssens, Jelle Leite, José Paulo Gagliardi |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Rotavirus / genética Genoma Viral |
topic |
Rotavirus / genética Genoma Viral |
description |
G12 group A rotavirus (RVA) are currently recognized as a globally emerging genotype and have been described in combination with several P-types. In Brazil, G12 RVA strains have been described in the Southern (2003) and Northern (2008 2010) regions, in combination with the P[9] and P[6] genotype, respectively. To date, few complete genomes of G12 RVA strains have been described (none from Brazilian strains), considering G12P[9] genotype just one strain, RVA/Human-tc/THA/T152/1998/G12P[9], has their 11 gene segments characterized. This study aims to determine the genomic constellation of G12P[9] and G12P[8] RVA strains detected in Brazil between 2006 and 2011. Therefore, the eleven gene segments of five Brazilian G12 RVA strains were amplified and sequenced, and the genotype of each gene segment was assigned using phylogenetic analysis. Complete genome analyses of G12 RVA strain circulating between 2006 and 2011 in Brazil revealed a conserved Wa-like genomic constellation for three G12P[8] RVA strains; whereas the two G12P[9] strains possessed distinct reassorted AU-1-like genomic constellations, closely related to the reference strain RVA/Human-tc/THA/T152/1998/G12P [9] in most genes. The results obtained in the current study suggest that G12P [9] (AU-1-like) and G12P[8] (Wa-like) strains detected in different regions of Brazil do not share a common origin. Moreover, while Brazilian G12P[8] RVA strains showed a complete Wa-like human constellation, both G12P[9] strains possessed an NSP1 gene of bovine origin (NSP1), and RVA/Human-wt/BRA/PE18974/2010/G12P[9] also possessed a VP3 gene of canine/feline origin. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014 |
dc.date.accessioned.fl_str_mv |
2016-01-26T11:39:53Z |
dc.date.available.fl_str_mv |
2016-01-26T11:39:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
GÓMEZ, Mariela M. et al. Distinct evolutionary origins of G12P[8] and G12P[9] group A rotavirus strains circulating in Brazil. Infection Genetics Evolution, v. 28, p. 385-388, 2014. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/981 |
dc.identifier.issn.-.fl_str_mv |
1567-7257 |
dc.identifier.doi.-.fl_str_mv |
10.1016/j.meegid.2014.04.007 |
identifier_str_mv |
GÓMEZ, Mariela M. et al. Distinct evolutionary origins of G12P[8] and G12P[9] group A rotavirus strains circulating in Brazil. Infection Genetics Evolution, v. 28, p. 385-388, 2014. 1567-7257 10.1016/j.meegid.2014.04.007 |
url |
https://patua.iec.gov.br/handle/iec/981 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
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